• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.161-167
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• 2007

In this study, we constructed and tested retrovirus vectors designed to express the human thrombopoietin gene under the control of the tetracycline-inducible promoters. To increase the hTPO gene expression at him-on state, WPRE sequence was also introduced into retrovirus vector at downstream region of either the hTPO gene or the sequence encoding reverse tetracycline-controlled transactivator (rtTA). Primary culture cells (PFF, porcine fetal fibroblast; CEF, chicken embryonic fibroblast) infected with the recombinant retrovirus were cultured in the medium supplemented with or without doxycycline for 48hr, and induction efficiency was measured by comparing the hTPO gene expression level using RT-PCR, western blot and ELISA. Higher hPTO expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hTPO (in CEF) or rtTA(in PFF) gene. This resulting tetracycline inducible vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.5031-5041
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• 2014

Purpose: The influence of the hoping number of children, the policy of childbirth, and the causes of reduced childbirth to the perception of childbirth, which will be the subject of future childbirth, was surveyed by health-line college students. Results: In terms of the attitude to the perception of childbirth, the quality of personal life, childcare, insufficient support of childbirth, and breeding of descendants had the largest influence on childbirth, and in terms of the attitude to the hoping number of children, economic reasons, childcare expenses, and education expenses had the largest influences. Second, when the causes of social demographic properties influencing childbirth was analyzed, sex showed significance to all the factors on the hoping number of children in terms of growing areas, to all the factors excluding childbirth policy in terms of the economic level, and hoping number of children in terms of the parents' jobs. Third, when the correlation was analyzed, a correlation was found in every area excluding the hoping number of children in terms of the causes of childbirth reduction and economy, childbirth policy in terms of the personal causes, personal factor in terms of the causes of childbirth policy, and family factor in terms of the causes of childbirth reduction and economy. Fourth, when the influences of the causes of hoping number of children, childbirth policy, and childbirth reduction to perception of childbirth were analyzed, the case hoping no or one child, the case hoping 2 or more children, childbirth policy, and childbirth reduction had a positive significant influence on the perception of childbirth. Conclusion: The childbirth rate will be improved if the following occur: childbirth policy and laws are established at the level of government, education programs are developed and operated for the formation of the value of perception of childbirth, and a new support policy is established after analyzing the current childbirth support policies. To change the perception of childbirth in college students who are future subjects of childbirth, successive studies will be needed to verify the effect of aforesaid measures.

• Reproductive and Developmental Biology
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• v.39 no.1
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• pp.13-21
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• 2015

The importance of genetic resource preservation has been highlighted in the literature as a means of maintaining genetic diversity. Investigations for hematologic values and the differential count of white blood cell count (WBC) for Korean indigenous cattle (KIC) and endangered indigenous cattle (EIC) are rarely performed. Therefore, the objective of this study was to investigate the hematologic values of total 40 EIC (White, Black, Mini cattle) and 35 KIC as control by analysis of hematologic characteristics. As a result, the mean values of RBC and platelet of EIC were significantly decreased by age (p<0.05). The mean values of RBC, HCT, MCV and MCHC between EIC and KIC of the same age (2~3 years) showed the statistical significance (p<0.05). Also, in the WBC of EIC, the mean values were decreased according to the age from $13.9{\times}10^3/{\mu}L{\sim}12.7{\times}10^3/{\mu}L$ under 1 year to $9.1{\times}10^3/{\mu}L{\sim}11.5{\times}10^3/{\mu}L$ over 2 years respectively. In the differential count of WBC of EIC (White, Black, Mini cattle), it showed generally the rates of 40.2%, 52.2%, 49.0% lymphocyte and 27.2%, 33.9%, 32.0% segmented neutrophil from 2~3 years respectively. Result of this study will be used for establishing reference range for blood analysis in EIC such as white, black and mini cattle. This study reported hematological values which could serve as baseline information for comparison in conditions of nutrient deficiency, physiological and health status of endangered Korean native cattle. In addition, this study provides a valuable resource for further investigations of the preservation of rare genetic stocks underlying traits of interest in cattle.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.711-724
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• 2003

Some growth curve models were used to fit individual growth of 1,083 Hanwoo cows born from 1970 to 2001 in Daekwanryeong branch, National Livestock Research Institute(NLRI). The effects of year-season of birth and age of dam were analyzed. In analysis of variance for growth curve parameters, the effects of birth year-season were significant for mature weight(A), growth ratio(b) and maturing rate(k)(P〈.01). The effects of age of dam were significant for growth ratio(b) but not significant for mature weight(A) and maturing rate(k). The linear term of the covariate of age at the final weights was significant for the A(P〈.01) and k(P〈.01) of Gompertz model, von Bertalanffy model and Logistic model. For the growth curve parameters fitted on individual data using Gompertz model, von Bertalanffy model and Logistic model, resulting the linear contrasts(fall-spring), Least square means of A in three nonlinear models were higher cows born at fall and A of Logistic model was significant(P〈.05) between the seasons. According to the results of the least square means of growth curve parameters by age of dam, least square means of mature weight(A) in Gompertz model was largest in 6 year and smallest estimating for 3 and 8 years of age of dam. The growth ratio(b) was largest in 2 year of age of dam and smallest estimating in 8 year. The A and k were not different by age of dam(p〉.05), On the other hand, the b was different by age of dam(p〈.01). The estimate of A in von Bertalanffy model was largest in 6 year and smallest in 8 and 9 years of age of dam. The b was largest in 2 year and tend to decline as age of dam increased. The A and k were not different by age of dam(p〉.05), On the other hand, the b was highly significant by age of dam(p〈.01).

• Korean Journal of Environmental Agriculture
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• v.31 no.4
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• pp.313-317
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• 2012

BACKGROUND: Citrus is usually propagated by grafting onto a rootstock. In Korea, As trifoliate orange (Poncirus trifoliata) has dwarf and strong cold hardness, it is widely used as the rootstock of satsuma mandarin. Because 'Shiranuhi' ((Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata), a kind of citrus, also, generally is grafted onto a trifoliate orange, most of farmer has been recognized that 'Shiranuhi' root is naturally trifoliate orange. Meanwhile, reduction of flowering in 'Shiranuhi' orchard has been issued among the farmers and researchers over past few years and they guessed it was occurred by severe prune, oversupply of fertilization, overfruiting and temperature of growth period. However, a few researchers strongly assumed that it would be caused by scion rooting of 'Shiranuhi'. So, this study was carried out to identify the existence of scion rooting in 'Shiranuhi' tree in Korea. METHODS AND RESULTS: To identify the existence of scion rooting in 'Shiranuhi' tree, we randomly selected six 'Shiranuhi'orchards and we surveyed three to four trees, which flowering was not enough, from six 'Shiranuhi' orchards respectively. We took the root samples of 'Shiranuhi' mandarin, and then separated the two group which were non-scion rooting (Trifoliate orange), and scion rooting ('Shiranuhi' mandarin). To identity the scion rooting, we used primer set of three types which were a F2/R15, F4/R15 and F5/R15 primer set. As a result, when we conducted the DNA analysis, fourteen tree in less bloomed twenty tree was proved as tree with the scion rooting of 'Shiranuhi' mandarin. CONCLUSION(S): Scion roots of 'Shiranuhi'mandarin were usually observed in a deeply planted tree, and xylem of 'Shiranuhi' root indicated more white color than a case of trifoliata orange. 'Shiranuhi' tree by scion rooting was more vigorous but less flowering than trees grafted onto trifoliata orange. When we used F2/R15, F4/R15 and F5/R15 primer set for discriminance of 'Shiranuhi'mandarin root and trifoliate root, we identified the existence of scion rooting in 'Shiranuhi', From our results, it is suggested that the influence of scion root should be reviewed in 'Shiranuhi'orchards.

• Korean Journal of Environment and Ecology
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• v.23 no.1
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• pp.30-40
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• 2009

Employing the Numata-type life form, the paper analyzed changes of plants for 4 years at the Human-made Wetlands along Boknaecheon of Juam Lake since its creation. The number of the species planted at the Human-made Wetlands along Boknaecheon of Juam Lake, which was completed in December 2002, were 15 in total including, 10 families, 13 genera, 12 species and 3 varieties. As for the three-featured life forms of the planted species, there were 6 perennial hydatophytes, recording the biggest number of species in dormancy form; species each of $R_5$, $R_3$, $R_{2-3}$ respectively in radicoid from; 20 species of geomantic dissenminule form ($D_1$) in disseminule form and erecred type(e) existed the most in growth form. With regard to the 3 features of life form identified during the final year of the monitoring that lasted 5 years after the completion of the Wetlands, the number of species and individuals was found to have increased but there was no significant change of tendency as against the composition ration(%) of life form. There were 43 species of therophytes (Th) that covered 24.29% in dormancy form, while $R_5$ was prevalent in radicoid form and $D_4$, $D_1$, and $D_{1,4}$ comprosed 77.39% of the whole disseminule form. Growth form was surveyed in the order of erected type (e), bunch type (t), temporal rosette type (pr), branch type (b) and straight rosette type (ps) and these species comprised 64.97% of the whole flora. Consequently, in case of the artificial wetlands along the Boknaecheon of Juam Lake, it turned out that the vegetation type in which pioneer species of succession, or gradually stabilized perennial vegetation favoring Wetlands because the higher dormancy form has its perennial plants' composition ratio getting, the more its succession is progressing. Even though single grained plants ($R_5$) belonging to radicoid in breeding form, succession is predicted to take place considering the fact that they actually belong to ~ plants like Phragmites japonica that form a connection on the surface of the earth. In addition, it is judged that geomantic disseminule form ($D_1$) conveyed by water and gravitational disseminule form favored by the development of waterside woody plants ($D_4$) seem to be better fit to this area in desseminule form. As for growth form, bunch type (t) is judged to become prevalent on the Wetlands while a good variety of phanerophytes will coexist on the earth due to artificial as well as natural disturbances.

• Journal of Bio-Environment Control
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• v.27 no.4
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• pp.341-348
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• 2018

To investigate the effect of light sources on the growth and photosynthesis of the dwarf apple rootstock M.9 for the production of standard seedlings, the plants were cultivated in a controlled environment for 6 weeks. The sources of light are six treatments [Red (R), Blue (B), White (W), RBUV (R7B3 containing UV-A), RBW (R3B1W1), SMF (high pressure sodium + metal halide + fluorescent lamp) under $154{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Growth characteristics of apple seedlings varied depending on artificial light source at 3 weeks and 6 weeks. The plant height of apple seedling was high in the R, RBUV, RBW, and SMF light sources at 3 weeks, and in the R light at 6 weeks. There was no significant difference on stem diameter among the treatments at 3 weeks, but showed high in RBUV and RBW light at 6 weeks. Leaf number was the highest in RBUV light at 3 and 6 weeks. The chlorophyll content (SPAD value) was high in the B and RBUV light at 3 weeks, but it was not significant at 6 weeks. The growth rate to height of the R light (1.12mm/day) was the highest among the treatments, followed by RBUV, RBW, SMF, W and then B. Leaf area was the highest in RBUV and RBW lowest in B. Specific leaf area was high in W and fresh and dry weight were high in RBUV. The photosynthetic rate at 6 weeks was highest in the B and lowest in the R. Stomatal conductivity and transpiration rate were higher in the B and W compared to the other light sources. Therefore, we are considered that light sources for growth of dwarf apple rootstock M.9 seedlings are suitable the R, RBUV, and RBW light sources with a high mixing ratio of Red and Red +Blue.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.3
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• pp.1-15
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• 2018

This study aims to investigate an aspect of physical palace by reviewing the emergences of tigers and leopards in the palace in Hanyang from the environmental historical perspective. To do so, three topics in environmental history were reviewed including 'environment,' 'impact of human-beings on the environment,' and 'sense and attitude of human-beings on the environment' based on the historical records such as "'Wangjoshilok(王朝實錄)". The results of this study are as follows. First, It was confirmed that leopards and tigers were emerged not only in the palace but also near the City Wall of Hanyang from time to time in Joseon Dynasty. Moreover, tigers lived and propagated in the backyard. Second, This situation may be caused by the natural factors of palace in Hanyang including location, philosophy and technique of building, and so on. That means 'good location' placed by palace worked favorably not only for human-beings but also for the activities of tigers and leopards. Third, In addition to the locational environment, forest policies on the palace and capital may facilitate the emergences of leopards and tigers. With the managements of pine trees, prohibition of burials, prohibition of climbing, prohibition of mountain entrance, and so on, forests were developed in the mountainous territories, which might play a role of shelters or passages for the tigers and leopards. Fourth, It was confirmed that countermeasures on the emergences of tigers in the palace as well as the capital city were different by the kings. Out of these, Jeong-jo was highlighted to seek the realistic countermeasures with actual understanding on the emergences of tigers and leopards. Fifth, In summary, frequent emergences of tigers and leopards in the palace and capital city could be a practical evidence to show 'pro-naturalism' or 'eco-friendliness' of the palace in Joseon which was mentioned superficially, paradoxically speaking. Further studies with the related disciplines are required on the cultural or ecological history of the palace. In addition, it is considered to conduct more detailed scientific studies related to the trend of ecological changes in Joseon.

• Journal of Korean Society of Forest Science
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• v.108 no.3
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• pp.290-295
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• 2019

This study was carried out to investigate the possibility of establishing a reproductive system for the seed of Pedicularis hallaisanensis, which is in the endangered wild species II class in Korea. The seed of P. hallaisanensis is egg-shaped, and the seed coat is dark brown. The embryo was identified as a dwarf type by the seed section. The seed length was $0.47{\pm}0.07mm$, width $0.16{\pm}0.006mm$, and thickness $0.12{\pm}0.01mm$. The weight of one seed was $0.0003{\pm}0.0001mg$, and 1000 seeds weighed $4.59{\pm}0.02mg$. The degree of seed viability was 75.33% by the tetrazolium (TZ) assay. The highest germination rate of P. hallaisanensis seed was 71% after 4 weeks of storage at $4^{\circ}C$. However, the germination rate tended to decrease gradually over a longer storage period. The germination rates after 6 or 8 weeks of storage at $4^{\circ}C$ were 64% and 60%, respectively. We used two host plants, Artemisia princeps and Dendranthema zawadskii, to determine the effect of host plants on P. hallaisanensis seed germination. The germination of P. hallaisanensis mixed with A. princeps or D. zawadskii started at 53.5 and 62.5 days after sowing, respectively. We did not find any germination 164 days postsowing with both host plants. When A. princeps and D. zawadskii were used as host plants for P. hallaisanensis seed germination, P. hallaisanensis seed germination rates were 45.5% and 19.5%, respectively. The average time to germination was 70.2 days for A. princeps, and 46.8 days for D. zawadskii.

• Korean Journal of Food Science and Technology
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• v.53 no.3
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• pp.356-363
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• 2021

This study investigated the quality characteristics of anchovy sauce fermented using Nuruk to maintain a unique flavor, reduce fishy smell, and improve the fermentation rate. Six kinds of fermented fish sauces, including the control, fermentation using traditional Nuruk; SH Koji (Fs-A), JJ Koji (Fs-B), GJ Koji (Fs-C); and fermentation with improved Nuruk; Aspergillus luchuensis (Fs-D) and Aspergillus oryzae (Fs-E), were prepared. Samples were collected at 15 days intervals with 10% Nuruk added to raw anchovy and fermented at 25o C for 60 days. The free amino acids, especially glutamic acid content and amino nitrogen, were the highest in Fs-C, reflecting the high protease activity of Nuruk C (GJ). Regarding overall sensory evaluation, the control was the lowest, whereas Fs-C was highly evaluated among the sample groups. The addition of Nuruk not only shortened the fermentation period, but also increased the overall sensory level by adding umami and reducing fishy odor.