• Journal of the Korean Wood Science and Technology
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• v.30 no.1
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• pp.72-78
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• 2002

Cultivation of Pholiota adiposa on sawdust media and its mixed substrates with various agricultural by-products was attempted. The composition of each substrates were compared from the viewpoint of cultivation possibilities with the production of Pholiota adiposa. Populus deltoides showed the best production of the mushroom with 795 g, followed by Robinia pseudo-acacia and Quercus acutissima. The mixed sawdust media with 70% of Robinia pseudo-acacia and 30% of Populus deltoides showed the best production of the mushroom with 265 g followed by the mixture of Robinia pseudo-acacia and Populus deltoides with 50% each with 228 g, while the addition of Quercus acutissima showed the negative effect for the production of Pholiota adiposa. Thus, Robinia pseudo-acacia was considered to be an effective amendment for the production of the mushroom. In addition, various kinds of agricultural by-products showed additive effect for the production of the mushroom, too. Therefore, we could concluded that the sawdust of Robinia pseudo-acacia and agricultural by-products can be used to cultivate Pholiota adiposa.

• The Korean Journal of Mycology
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• v.24 no.3 s.78
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• pp.223-227
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• 1996

The effects of addition of dried apple pomace to sawdust substrate on the growth of Flammulina velutipes were investigated during the period of 2 years $(1994{\sim}1995)$. Dried apple pomace used in this study consisted of 44.6% C, 0.54% N, 0.16% CaO, 0.16% $P_2O_5$, 0.11% MgO and 1.29% $K_2O$ (pH 4.4). The addition of 15% apple pomace (v/v) increased the yield of the mushroom fruitbody by 9%, and period of primordia formation was similar regardless of the treatments. In economical analysis, the addition of 15% apple pomace (v/v) increased 29% compared to the control treatment in relative income.

• Journal of agriculture & life science
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• v.46 no.3
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• pp.59-68
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• 2012

This study was conducted to investigate the economic analysis of fermented diet made of agricultural and marine by-products such as, dropped apples, persimmon shells, mushroom (Flammulina velutipes) by-products, king oyster mushroom by-products and brewer's grain in Berkshire or crossed pigs. The daily diet cost were decreased by 2.16~3.44%, 3.83~13.35% and 7.47~41.61% when fermented dropped apples diet, fermented persimmon shells diet and fermented mushroom (F. velutipes) by-products diet were fed to Berkshire pigs. The daily feed cost was also decreased by 1.59~4.05% and 59.09~66.90% when fermented king oyster mushroom by-products diet and fermented brewer's grain diet were fed to crossed pigs. Carcass prices were increased by 3.09%, 1.20~3.40% and 1.13% when 2% fermented dropped apples diet, fermented persimmon shells diet and 0.5~1.0% fermented mushroom (F. velutipes) by-products diet were fed to Berkshire pigs. Carcass prices were increased by 2.87~4.19% and 41.31~49.14% when fermented king oyster mushroom by-products diet and fermented brewer's grain were fed to crossed pigs. Therefore, fermented agro by-products diet of dropped apples, persimmon shells, king oyster mushroom, mushroom (F. velutipes) and brewer's grain was expected to improve swine farms income due to decreased diet cost and increased carcass prices in Berkshire pigs or crossed pigs.

• Journal of Animal Science and Technology
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• v.50 no.6
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• pp.831-838
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• 2008

This study was conducted to evaluate effects of mixed microbes addition on physico-chemical, fermentative and microbial parameters of sawdust-based spent mushroom substrates(SMS). The SMS was inoculated with mixed microbes(Enterobacter ludwigii, Bacillus cereus, 2 strains of Bacillus subtillis, Saccharomyces cerevisiae and Lactobacillus plantarum) at 1% level(wet basis) and anaerobically fermented during the different periods(up to 8th week). Compared with the SMS before ensiling, the ensiled one had higher CP, NDF and ADF percentages and lower DM and NFC percentages. However, levels of change were very low. The in situ ruminal disappearance of SMS DM and NDF decreased with the ensiling period prolonged. For fermentative parameters, pH reduced and lactic acid contents increased after ensiling, compared with those after ensiling. At 8th week of ensiling, pH increased and lactic acid contents reduced again, compared with those at 4th week of ensiling; however, the silage still showed favorable fermentation status. Lactic acid bacteria counts did not change throughout 8 weeks of ensiling. Counts of total microbes and yeast reduced after 4th week of ensiling period. Counts of lactic acid bacteria and yeast at 8th week of ensiling were in the levels of 108cfu/g. These results indicate that anaerobic fermentation with microbial addition could be an effective way for the long term(8 weeks) storage of the SMS.

• The Korean Journal of Mycology
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• v.26 no.1 s.84
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• pp.47-50
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• 1998

The cheaper and high-quality media are necessary to produce the year-round mushroom. For low input and high productivity, this experiment was carried out to screen the proper supplementary materials on media among agricultural by-products; bean-curd residues, dried orange peel, chinese drug residues and celery cabbage leaf. Judging by chemico-physicals, the used agricultural by-products was possible for an additives on mushroom media. Bean-curd residues added to media raised the amount of organic matter, nitrogen and carbon. In Pleurotus ostreatus, the conventional media yielded 20 pilus cap, 72g per a bottle, but adding 10 percent bean-curd residues produced 12 pilus cap, 77g and an increase yielded 7%, and 10 percent orange peel took 12 pilus cap and an increase yieled 11%. In Agrocybe aegerita, added to media, except chinese drug residues, increased performance compared with conventional media, produced 98g per a bottle. Especially, the bean-curd residue increase 15 percent in productivity.

• Proceedings of the Korea Technology Innovation Society Conference
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• 2013.10a
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• pp.798-834
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• 2013

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.410-419
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• 2018

It is urgently required to construct safety data on agricultural by-products imported for use as medium materials for domestic mushroom production. However, research on microorganisms is insufficient. This study was conducted to investigate the presence of bacteria that have the possibility of harmful effects on human, plants and mushroom in wheat straw, peatmoss, cottonseed hull, cottonseed meal, and beet pulp imported from Australia, Canada, China, Egypt, Germany. Bacteria were found in the range of $1.35{\times}10^2$ to $8.34{\times}10^6CFU/g$. As a result of 16S rDNA sequence analysis, total of 19 genera and 45 species of bacteria were identified. Bacillus genus was dominant, followed by Paenibacillus genus. At the species level, diverse species was in the order of Firmicute, Proteobacteria and Actinobacteria. Regarding the agricultural by-products, straw and peat moss had more diverse bacteria than other agricultural by-products. Among the indentified bacteria, 6 species of 5 genera (Enterobacter asburiae, Enterobacter ludwigii, Stenotrophomonas maltophilia, Pseudomonas monteilii, Bacillus anthracis, and Cellulosimicrobium funkei) were present as potent harmful bacteria to human. Surprisingly, both the human and plant pathogenic Klebsiella pneumoniae subsp. pneumonia was present. Bacillus altitudinis was present as a plant pathogen. Lysinibacillus sphaericus, an insect pathogen, and Ochrobactrum pseudogrignonense, a mushroom pathogen, were also present. The results of this study confirmed that several kinds of pathogenic bacteria were present in the agricultural by-products for the mushroom cultivation medium imported into Korea. Our work suggests that hygiene inspection and management is urgently needed for imported agricultural by-products to be safely used for mushroom production.

• Journal of Mushroom
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• v.6 no.3_4
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• pp.126-130
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• 2008

The combination ratio of oyster mushroom cultivation supplements have used poplar sawdust(PS) 50% : beet pulp(BP) 30% : cotton seeds meal(CS) 20%. we substituted cotton seeds meal and beet pulp for food by-product dried wastes(DW) 10, 20. 30% respectively. In case of mixing with DW 10% except from BP, mycelial growth was inhibited a little, but mycelial density was accelerated. The more DW contents increase, the more mycelial growth was inhibited, but mycelial growth was accelerated a little, respectively. In case of mixing with DW except from CS, mycelial growth was similar to control almost. In case of mixing with DW except from CS and BP, mycelial growth was inhibited remarkably. CHUNCHU variety developed resistance to developed than SUHAN variety.

• Journal of Mushroom
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• v.15 no.1
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• pp.21-24
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• 2017

In this study, the effect of different substrates of agricultural by-products on the mycelial growth rate and density of Trametes versicolor (Turkeytail mushroom) was analyzed. We found that pepper stem and rice bran with a mixing ratio of 9:1(v/v) produced the best mycelial growth of 101 mm in 10 days, while a mixing ratio of 8:2 resulted in mycelial growth of 83 mm in 10 days. The control group treated with a 9:1 mixing ratio of oak sawdust and rice bran (v/v) produced mycelial growth of 74 mm in 10 days. The following results are in the order of beanstalk, sesame stem, and perilla stem. After the harvest of the mushrooms, the mycelial growth rate and the density of T. versicolor in each substrate were as follows the group with waste substrate of Pleurotus eryngii and rice bran with a mixing ratio of 9:1(v/v) produced the best result of 76 mm in days, while a mixing ratio of 8:2 produced of 61 mm in 10 days. The control group with a 9:1 ratio of oak sawdust and rice bran produced mycelia of 74 mm in 10 days, while a mixing ratio of 8:2 resulted in mycelia of 59 mm in10 days.

• Journal of Mushroom
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• v.10 no.4
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• pp.236-243
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• 2012

Spent mushroom substrates is composted organic material remaining after a crop of mushroom is harvested. The raw materials of mushroom substrates are same feed ingredient as corncobs, rice brown, wheat brown, cotton seeds and beet pulp. During the mushroom cultivation process, the mushroom substrates was used 15-25% by mushroom and 75-85% of mushroom substrates was remained in the SMS. Among of the spent mushroom substrates, spent mushroom substrates of pleurotus eryngii, Pleurotus ostreatus and Flammuliua velutipes is can be use the energy feedstock of animal feed. The cellulose content of spent mushroom(pleurotus eryngii) substrates containing the sawdust was high and total digestible nutrients (TDN) values was low. The spent mushroom(pleurotus eryngii) substrates fermented with cellulase and xylanase producing bacteria is may be used as an ingredient of feed in TMR for Hanwoo steer.