Browse > Article
http://dx.doi.org/10.5187/JAST.2008.50.6.831

Effect of Mixed Microbes Addition on Chemical Change and Silage Storage of Spent Mushroom Substrates  

Kim, Young-Il (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Seok, Joon-Sang (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Kwak, Wan-Sup (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Publication Information
Journal of Animal Science and Technology / v.50, no.6, 2008 , pp. 831-838 More about this Journal
Abstract
This study was conducted to evaluate effects of mixed microbes addition on physico-chemical, fermentative and microbial parameters of sawdust-based spent mushroom substrates(SMS). The SMS was inoculated with mixed microbes(Enterobacter ludwigii, Bacillus cereus, 2 strains of Bacillus subtillis, Saccharomyces cerevisiae and Lactobacillus plantarum) at 1% level(wet basis) and anaerobically fermented during the different periods(up to 8th week). Compared with the SMS before ensiling, the ensiled one had higher CP, NDF and ADF percentages and lower DM and NFC percentages. However, levels of change were very low. The in situ ruminal disappearance of SMS DM and NDF decreased with the ensiling period prolonged. For fermentative parameters, pH reduced and lactic acid contents increased after ensiling, compared with those after ensiling. At 8th week of ensiling, pH increased and lactic acid contents reduced again, compared with those at 4th week of ensiling; however, the silage still showed favorable fermentation status. Lactic acid bacteria counts did not change throughout 8 weeks of ensiling. Counts of total microbes and yeast reduced after 4th week of ensiling period. Counts of lactic acid bacteria and yeast at 8th week of ensiling were in the levels of 108cfu/g. These results indicate that anaerobic fermentation with microbial addition could be an effective way for the long term(8 weeks) storage of the SMS.
Keywords
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 정원형, 양시용, 송민동, 하종규, 김창원. 2003. Xylanase, Cellulase의 생산성이 높은 Bacillus sp.의 분리 및 효소생산을 위한 배지조건의 최적화. 한국미생물생명공학회지. 31(4):383-388
2 Yang, S. Y., Ji, K. S., Baik, Y. H., Kwak, W. S. and McCaskey, T. A. 2006. Lactic acid fermentation of food waste for swine feed. Bioresou. Technol. 97:1858-1864   DOI   ScienceOn
3 Yoon, I. K. and Stern, M. D. 1995. Influence of direct-fed microbials on ruminal microbial fermentation and performance of ruminants: a review. Asian Aust. J. Anim. Sci. 8(6):533-555   DOI
4 김영일, 배지선, 정세형, 안문환, 곽완섭. 2007a. 버섯폐배지의 발생량 조사 및 새송이, 느타리, 팽이 버섯 폐배지의 버섯종류별과 재배방식별의 물리화학적 특성평가. 한국동물자원과학회지. 49 (1):79-88   DOI
5 김영일, 배지선, 허정원, 곽완섭. 2007b. 버섯의 봉지재배 및 병재배 시 재배단계별 배지의 사료영양적 성분, 독성중금속 및 잔류농약 모니터링. 한국동물자원과학회지. 49(1):67-78   DOI
6 Kwak, W. S., Jung, S. H. and Kim, Y. I. 2008. Broiler litter supplementation improves storage and feed-nutritional value of sawdust-based spent mushroom substrates. Bioresou. Technol. 99(8): 2947-2955   DOI   ScienceOn
7 Lee, K. S., Lee, K. Y., Oh, C. S., Lee, D. G. and Kim. Y. J. 2004. Effect of aeration for the probiotic feed production from food wastes by Lactobacillus acidophilus and Saccharomyces cerevisiae. J. KOWREC. 11(4):114-119
8 Nocek, J. E. 1985. Evaluation of specific variable affecting in situ estimates of ruminal dry matter and protein digestion. J. Anim. Sci. 60:1347-1358   DOI
9 Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991. Methods of dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3582-3597
10 AOAC. 1990. Official Methods of Analysis, 14th Ed. Association of Official Analytical Chemists, Washington, D. C., USA
11 Armentano, L. E., Herrington, T. A., Polan, C. E., Moe, A. J., Herbein J. H. and Umstadt, P. 1986. Ruminal degradation of dried brewers grains, wet brewers granins and soybean meal. J. Dairy Sci. 69:2124-2133   DOI
12 Barker, S. B. and Summerson, W. H. 1941. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-554
13 Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356   DOI
14 Kwak, W. S., Kim, Y. I., Seok, J. S., Oh, Y. L., and Lee, S. M. 2009. Molasses and microbial inoculants improve fermentability and silage quality of cotton waste-based spent mushroom substrate. Bioresou. Technol. 100(3):1471-1473   DOI   ScienceOn
15 SAS Institute, Inc., 1990. SAS User's Guide, Version 6.08, Fourth Ed., SAS Inst., Inc., Cary, NC. USA
16 김영일, 정세형, 양시용, 허정원, 곽완섭. 2007c. 버섯부산물 퇴적발효 시 섬유소 분해균 접종이 섬유소 분해성 효소 활력과 면양의 영양소 이용성에 미치는 영향. 한국동물자원과학회지. 49(5): 667-676   DOI
17 양시용, 송민동, 김언현, 김창원. 2001. Probiotics용 복합효소 분비 Bacillus sp.의 분리 및 원료사료를 이용한 균주 생산을 위한 배지 조건의 최적화. 한국미생물생명공학회지. 29(2):110-114
18 Krehbiel, C. R., Rust, S. R., Zhang, G., and Gilliland, S. E. 2003. Bacterial direct-fed micro- bials in ruminant diets:Performance responses and mode of action. J. Anim. Sci. 81:E120-E132
19 Adamovic, M., Grubi, G., Milenkovic, I., Jovanovi, R., Proti, R., Sretenovi, L. and Stoievi, L. 1998. The biodegradation of wheat straw by Pleurotus ostreatus mushrooms and its use in cattle feeding. Animal Feed Science Technology 71:357-362   DOI   ScienceOn
20 Smith, L. W., Goering, H. K., Waldo, D. R. and Gordon, C. H. 1971. In vitro digestion rate of forage cell wall components, J. Dairy Sci. 54: 71-79   DOI
21 Caswell, L. E. 1990. Fungal additives. Feed Management. April. 41(4):9-13
22 Gao, L., Yang, H., Wang, X., Huang, Z., Ishii, M., Igarashi, Y. and Cui, Z. 2008. Rice straw fermentation using lactic acid bacteria. Bioresource Technology 99(8):2742-2748   DOI   ScienceOn
23 배지선, 김영일, 정세형, 오영균, 곽완섭. 2006. 느타리, 새송이, 팽이버섯 폐배지의 반추동물 조사료원으로서의 사료 영양적 가치평가. 한국동물자원과학회지. 48(2):237-246   DOI