• Journal of Veterinary Clinics
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• v.21 no.2
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• pp.87-92
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• 2004

This study was undertaken to compare the serological response of dogs to four commercially available combination vaccines and three different vaccination schedules to canine distemper virus (CDV). A total of 120 healthy puppies (20 puppies per group) at 6 weeks of age were randomly assigned to one of four vaccines [C, G, K, and V (or V3) groups] and one of vaccination schedules [V2 and V4 groups]. At six, nine, and 12 weeks of age, puppies in each group were vaccinated with one of four combination vaccines subcutaneously. And puppies in V2 and V4 groups were vaccinated with V vaccine every 2 weeks and 4 weeks, respectively. The serological responses to CDV component of the vaccines were determined by measuring SN titers. The immunogenicity of V vaccine was superior to the other vaccines and optimum vaccination schedule was 3 times vaccination with 3 weeks-interval starting vaccination at 6 weeks of age. Although puppies were vaccinated at 6 weeks of age, the geometric mean CDV titers of puppies in all groups by 9 weeks of age were under the protective level. Therefore, prophylactic measures should include isolation of young dogs from the dog population until vaccination can be expected to provide protection.

• Pediatric Infection and Vaccine
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• v.18 no.1
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• pp.68-79
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• 2011

Purpose : This post-marketing surveillance study (NCT00750360) assessed the safety and reactogenicity of an inactivated, trivalent split-virion influenza vaccine licensed for use in the Korea since 2002. Methods : Eight hundred and eighty three subjects aged 6 months received a single dose of the ${\geq}$ vaccine; an additional dose was administered to those aged <9 years and unprimed with an influenza vaccine. Four hundred and eleven subjects used diary cards to record safety information; this report presents data from these subjects. Incidence of solicited local, general and unsolicited adverse events (4-days and 21-days post-vaccination follow-up periods, respectively) were recorded. Serious adverse events (SAEs) were recorded throughout the study period. Results : Injection site pain (subjects aged <6 years: 12.6% of subjects, ${\geq}$6 years: 34.7%), fever (<6 years: 1.3%) and myalgia (${\geq}$6 years: 13.9%) were the most frequently recorded solicited local and general adverse events. Grade 3 solicited adverse events were reported by ${\leq}$4.0% subjects. No vaccine-related SAEs were recorded (KFDA criteria). Conclusion : Considering the vaccine's well-established immunogenicity and its favourable safety and reactogenicity profile across all age groups and its high coverage rate in Korea, it may be recommended as a candidate to facilitate annual seasonal influenza vaccination for all ages as part of the Korean National Immunization Program.

• Korean Journal of Poultry Science
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• v.33 no.4
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• pp.295-301
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• 2006

This study has been designed to evaluate the efficacy of MD in-ovo vaccination in broiler and to find the contamination level of MD virus in broiler farms. A nested PCR test was used to find the level of filed contamination of pathogenic MD virus in the commercial broiler farms. The positive rate of farms contaminated with pathogenic MD virus was 26.09%. And to evaluate the efficacy of MD vaccination, performance factors such as the mortality, feed conversion rate and body weight at market age has been recorded and compared among farms. There was no difference on these factors between vaccinated and non-vaccinated farms except performance. We need further investigation of the film with low performance index. The B/B ratio, which is common index for the evaluation of bursal changes, was calculated and compared between vaccinated and non-vaccinated group There were significant decreases of B/B ratio in the both group without any statistical difference between groups. The reason of decreased bursal size may be due to other infection such as IBD. From this study, we can know that the contamination rate of fm with pathogenic MD virus was very low and not different from previous study. However, the difference of the contamination rate in different locations recommends periodical monitoring on these areas.

• Pediatric Infection and Vaccine
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• v.25 no.1
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• pp.26-34
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• 2018

Purpose: This study aimed to investigate the changes in the occurrence of rotavirus gastroenteritis (RGE) after the introduction of rotavirus vaccine and estimate rotavirus vaccine effectiveness in hospitalized children. Methods: We compared the retrospective data of 671 patients with acute gastroenteritis (AGE) admitted to the Department of Pediatrics, Hanyang University Seoul Hospital from January 1, 2014, to December 31, 2015, with retrospective data of 1,243 patients admitted to the same institution with AGE from January 1, 2004, to December 31, 2005. The vaccine effectiveness was estimated using a case-positive control test-negative study. Results: The proportion of RGE in AGE was significantly lower in 2014 to 2015 (9.0%, 48/531) than in 2004 to 2005 (22.7%, 282/1,243) (P<0.001). In particular, there was a significant decrease in the 6- to 11-, 12- to 23-, and 24- to 35-month-old groups (P<0.001), whose rotavirus vaccination rates were higher than the remaining age groups. The monthly distribution of patients with RGE in 2004 to 2005 was higher from November to May, peaked in January, followed by December and February. In 2014 to 2015, the monthly distribution of patients with RGE slightly peaked in January. In 2014 to 2015 study, the complete rotavirus vaccination rate was 66.0% (332/503) and incomplete vaccination rate was 6.2% (31/503). Presumed rotavirus vaccine effectiveness was 83.3% (95% confidence interval [CI], 60.5% to 92.9%) in the complete vaccination group and 27.4% (95% CI, -163.7% to 80.0%) in the incomplete group. Conclusions: The proportion of RGE in AGE has markedly decreased since the introduction of rotavirus vaccines, and improving vaccination coverage will further reduce the burden of RGE in Korea.

• Journal of Life Science
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• v.32 no.5
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• pp.375-390
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• 2022

Influenza viruses are zoonotic respiratory pathogens, and influenza infections have caused a substantial burden on public health systems and the livestock industry. Although currently approved seasonal influenza vaccines have shown potent protection efficacy against antigenically well-matched strains, there are considerable unmet needs for the efficient control of viral infections. Enormous efforts have been made to develop broadly protective universal influenza vaccines to tackle the huge levels of genetic diversity and variability of influenza viruses. In addition, antiviral drugs have been considered important interventions for the treatment of viral infections. The viral neuraminidase inhibitor oseltamivir is the most widely used antiviral medication to treat influenza A and influenza B viruses. However, unsatisfactory clinical outcomes resulting from side effects and the emergence of resistant variants have led to greater attention being paid to plants as a natural resource for anti-influenza drugs. In particular, the recent COVID-19 pandemic has underpinned the need for safe and effective antiviral drugs with a broad spectrum of antiviral activity to prevent the rapid spread of viruses among humans. This review outlines the results of the antiviral activities of various natural products isolated from plants against influenza viruses. Special focus is paid to the virucidal effects and the immune-enhancing effects of antiviral natural products, since the products have broad applications as inactivating agents for the preparation of inactivated vaccines and vaccine adjuvants.

• Journal of the Korean Applied Science and Technology
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• v.38 no.6
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• pp.1722-1734
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• 2021

The purpose of this study was that HPV prevention education based on the planned behavioral theory was designed to provide college students with HPV vaccine knowledge, cervical cancer knowledge, attitude toward HPV vaccination, subjective norms for HPV vaccination, perceived behavioral control for HPV vaccination, and HPV vaccination. This is an experimental study to confirm the effect on the intention to vaccinate and the behavior of HPV vaccination. The subjects were arbitrarily expressed as 32 subjects in the experimental group and 34 subjects in the control group. The collected data were analyzed by descriptive statistics, t-test, χ²-test, Fisher's exact test and repeated Measures ANOVA. As a result, the experimental group participating in HPV prevention education was compared to the control group who did not participate in HPV vaccine knowledge (t=5.66, p<.001), cervical cancer knowledge (t=4.13, p<.001), attitude (t=2.24, p=) .032 ), subjective norm (t=2.83, p=.008), perceived behavioral control (t=2.65, p=.013), and intention (t=3.91, p<.001) were significantly different. After 4 weeks of HPV prevention education intervention, there was a significant difference in the interaction between group and time course of HPV vaccination intention (F=6.95, p=.002). Therefore, it was confirmed that HPV prevention education is an educational program that can be applied to college students.

• Korean Journal of Poultry Science
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• v.19 no.3
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• pp.151-160
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• 1992

Control of coccidiosis depends on medication, management, immunity, or some combination of these methods. Prophylactic medication with anticoccidials remains the primary method of control, although the development of drug resistance is a major problem. Shuttle and rotation programs are increasingly used to delay the onset of drug resistance. Sensitivity testing has become an important tool in the identification of what specific medications will be effective in specific poultry operations. Management techniques for the control of coccidiosis have not been totally effective because most disinfectants do not kill the coccidial oocysts. Present methods for vaccination are currently limited to the use of controlled doses of live coccidia. New vaccination methods under investigation include vaccination with attenuated strains or parasite antigens produced either by fractionating the parasites or genetically engineered sub unit vaccines.

• KOREAN POULTRY JOURNAL
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• v.36 no.8 s.418
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• pp.104-109
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• 2004

지난해 12월 국내 첫 HPAI발생 후, 방역당국의 발생지역 반경 3km내의 살처분에 대하여 우리는 처음부터 외국의 연구자료와 발생대처사례, 국제기구(FAO, OIE, WHO)의 권고 등을 참고하여 불행하게도 감염된 농장은 살처분 하더라도, 차단방역을 충실하게 실시한 미 감염농장만이라도 국가 관리하에 백신을 사용하여, 무차별 살처분으로 인한 억울한 농가피해와 매립으로 인한 인력동원, 환경오염, 막대한 국가재정보상부담을 줄일 수 있는 살처분과 백신병용(이하 백신 병용이라함)정책을 채택해 줄 것을 제의해왔다. 그러나 방역당국의 대책위에 참여하고 있는 교수(이하 방대위라 함)들은 이에 대해 지속적으로 반론을 제기하며, 심지어 관련 학자들의 인격문제까지 거론한다면서, 이에 대한 공청회를 요청(양계연구 4월호)하여 이에 답하고자 한다. 우선 우리는 공청회 요청에 대해 기꺼이 응할 수 있음을 전하고 싶다. 그러나 공청회는 정부가 정책을 추진하기 위해, 이해 당사자들의 의견을 수렴하기 위한 것이므로, 양계농가와 양계협회가 주관하여 관련 교수들과 소득 없는 논쟁에 그쳐서는 안되며, 이 중차대한 국가대사에 반드시 정부관련 부처가 주관하여, 가능하면 입법부 관련 의원들까지 함께 하는 자리에서 이해당사자인 백신병용주장농가와 살처분고수학자 및 관련당사자가 참여하여 충분한 토의를 거쳐, 향후 HPAI 재발시 반드시 방역정책에 반영될 수 있는 계기가 되어야 한다고 생각한다. 또 공청회 개최를 요구하면서, 이어 필자가 관련신문 및 월간지를 통해 백신병용을 요구한 기고문에 대하여, 장론의 반론을 제기함으로(양계연구 4월호), 이에 대해 덧붙여 답변하고자 한다.

• Journal of Life Science
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• v.32 no.12
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• pp.919-928
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• 2022

As seen in the COVID-19 pandemic, unexpected emergence of new viruses presents serious concern on public health. Especially, the absence of effective vaccines or antiviral drugs against emerging viruses significantly increases the severity of disease and duration of viral circulation among population. Natural products have served as a major source for safe and effective antiviral drugs. In this study, we examined the virucidal activity of medical herb extracts with a view to discover novel antiviral agents with desired levels of safety and antiviral efficacy. Ethanol extracts of ten selected medical herbs were tested for antioxidant activity and in-vitro cytotoxicity in various animal cell lines. Of note, the herbal extracts showed broad and potent virucidal activities against rotavirus, hepatitis A virus, and influenza A virus. The extracts of Sorbus commixta and Glycyrrhiza uralensis showed strong virucidal activities against influenza A virus. We also examined whether the extracts of Sorbus commixta and Glycyrrhiza uralensis can be used as inactivating agents to prepare an inactivated viral vaccine. In a mouse model, influenza A virus inactivated by the extracts elicited high levels of neutralizing antibodies, and the vaccination provided complete protection against lethal challenge. These results suggest that herb-derived natural products can be developed to antiviral drugs as well as inactivating agents for preparation of inactivated viral vaccines.

• The Science & Technology
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• v.33 no.2 s.369
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• pp.18-19
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• 2000

포항공대 성영철 교수는 최근 에이즈의 DNA백신을 개발, 원숭이에서 방어면역이 발생함을 증명해내 에이즈 완치에 청신호가 되고 있다. 세계 최초로 개발에 성공한 이 이에즈 DNA백신은 미국 식품의약국(FDA)에서도 안전하다고 인정하고 있는 순수 DNA만으로 제조된 것이어서 사람에 대한 임상실험이 신속히 이루어질 수 있는 것이다. 이번 연구결과는 이미 특허출원했으며 소동물(생쥐) 실험결과는 백신분야의 세계적 권위지 "vaccine"지에 발표되었다.