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http://dx.doi.org/10.5352/JLS.2022.32.12.919

Preparation of an Inactivated Influenza Vaccine Using the Ethanol Extracts of Medical Herbs  

Cho, Sehee (Department of Biological Science and Biotechnology, Andong National University)
Lee, Seung-Hoon (Gyeongbuk Institute for Bio Industry)
Kim, Seonjeong (Department of Biological Sciences and Biotechnology Major in Bio-Vaccine Engineering, Andong National University)
Cheong, Yucheol (Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University)
Kim, Yewon (Department of Biological Sciences and Biotechnology Major in Bio-Vaccine Engineering, Andong National University)
Kim, Ju Won (Department of Biological Sciences and Biotechnology Major in Bio-Vaccine Engineering, Andong National University)
Kim, Su Jeong (Department of Biological Sciences and Biotechnology Major in Bio-Vaccine Engineering, Andong National University)
Seo, Seungin (Gyeongbuk Institute for Bio Industry)
Seo, Dong-Won (Gyeongbuk Institute for Bio Industry)
Lim, Jae-Hwan (Department of Biological Science and Biotechnology, Andong National University)
Jeon, Sejin (Department of Biological Sciences and Biotechnology Major in Bio-Vaccine Engineering, Andong National University)
Jang, Yo Han (Department of Biological Sciences and Biotechnology Major in Bio-Vaccine Engineering, Andong National University)
Publication Information
Journal of Life Science / v.32, no.12, 2022 , pp. 919-928 More about this Journal
Abstract
As seen in the COVID-19 pandemic, unexpected emergence of new viruses presents serious concern on public health. Especially, the absence of effective vaccines or antiviral drugs against emerging viruses significantly increases the severity of disease and duration of viral circulation among population. Natural products have served as a major source for safe and effective antiviral drugs. In this study, we examined the virucidal activity of medical herb extracts with a view to discover novel antiviral agents with desired levels of safety and antiviral efficacy. Ethanol extracts of ten selected medical herbs were tested for antioxidant activity and in-vitro cytotoxicity in various animal cell lines. Of note, the herbal extracts showed broad and potent virucidal activities against rotavirus, hepatitis A virus, and influenza A virus. The extracts of Sorbus commixta and Glycyrrhiza uralensis showed strong virucidal activities against influenza A virus. We also examined whether the extracts of Sorbus commixta and Glycyrrhiza uralensis can be used as inactivating agents to prepare an inactivated viral vaccine. In a mouse model, influenza A virus inactivated by the extracts elicited high levels of neutralizing antibodies, and the vaccination provided complete protection against lethal challenge. These results suggest that herb-derived natural products can be developed to antiviral drugs as well as inactivating agents for preparation of inactivated viral vaccines.
Keywords
Antibody; antiviral drug; inactivated vaccine; medical herb; natural product;
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