• Development and Reproduction
• /
• v.2 no.1
• /
• pp.81-87
• /
• 1998

A scanning electron microscopic study on the glochidial encystment and excystment during the development of Anodonta arcaeformis flavotincta on Carassius auratus, a common natural host fish, was carried out. The glochidia were attached to the fins, buccal cavity and gills of the host fish within 30 minutes. In this study, the fins of host fish infected with the glochidia were examined in a time series. The attachment rates of the glochidia on the pectoral fins, caudal fin and pelvic fins of the host fish were 30%, 22%, and 17%, respectively. The glochidia which attached to the fish became encysted within 27 hrs. The process of encystment progressed slowly. Ti took 24 to 27 hours in the formation of the primary cyst, and after 5 to 6 days, the larvae was covered completely with the epithelial cels of the host tissues. The process of detachment of juvenile clam was observed on the 8th day after host infection. Most of the juvenile clams have sloughed from the cyst of the host within 15 days. No significant size difference was observed in the glochidia and the juvenile which were found before attachment and after detachment from the cyst of the host fish.

• Horticultural Science & Technology
• /
• v.33 no.4
• /
• pp.518-524
• /
• 2015

The objective of this research was to investigate the influence of various depths of expanded rice hull (ERH) medium and fixation dates of runner plantlets of 'Seolhyang' strawberry on the growths in nursery field and in plastic house soil after transplanting. The five treatments in medium depths (30, 50, 70, 90, and 110 mm) and four treatments in fixation dates (1st and 15th July and 1st and 15th August) were tested. The growths of runner plantlets were investigated before transplanting to plastic house soil. The early growth and inflorescence rates of crops after transplant to plastic house soil were also investigated. The plant height and fresh weight of runner plantlets were the highest in the medium depths of 50, 70, and 90 mm. The medium depth of 30 mm had higher numbers of first roots, but lower root fresh weight compared to those of 70, 90, and 110 mm. The treatment of 30 mm in medium depth showed poorer growth in all indexes except root length and root weight compared to those of 70, 90, and 110 mm. The runner plantlets fixed on July 1 and July 15 showed good root growth and the weights of ERH adhered to form root balls were 18.3 g and 13.9 g, respectively. The detached amount of ERH was less than 40% in the two treatments when root balls were shaken by a vibratory sieve shaker. The plant growth at 45 days after transplanting to plastic house soil were not significantly different when the runner plantlets were fixed in the period from July 1 to Aug. 1. The inflorescence rates of the first cluster were 93 to 100% when runner plantlets were fixed in the period from July 1 to Aug. 1. By contrast the runner plantlets fixed on the Aug. 15 had a 67% in florescence rate for the first cluster. These results indicate that optimum depth of ERH medium was 7 cm and the ranges of optimum fixation dates are from July 20 to 25.

• Applied Microscopy
• /
• v.39 no.2
• /
• pp.117-124
• /
• 2009

Clonorchis sinensis is the most important widely distributed parasite of the human bile duct in East Asia and the most prevalent parasitic helminth in Korea. The prevalence rate of human clonorchiasis has remained at about 2.9% in Korea. C. sinensis induces dilatation of the duct, hyperplasia of the mucosa, metaplasia or neoplasia of the mucosal epithelium, periductal inflammation and fibrosis, and thickening of the ductal wall. Fibroblast are the most common cells in connective tissue and are responsible for the synthesis of extracellular matrix components. The fibrosis associated with chronic inflammation and injury may also contribute to cholangiocarcinoma pathogenesis, particularly through an increase in extracellular matrix components, which participate in the regulation of bile duct differentiation during development. In this study, ultrastructural changes, the distribution of lectin receptors and actin protein in cultured SD rat bile duct fibroblast after infection of C. sinensis were observed. Experimental group had been divided into four groups: normal bile duct fibroblast cultured in basal media (G1); C. sinensis infected bile duct fibroblast cultured in basal media (G2); normal bile duct fibroblast cultured in basal media containing excretory-secretory product (ESP) (G1-1); C. sinensis infected bile duct fibroblast cultured in basal media containing ESP (G2-1). Overall, once a host is infected by C. sinensis, it affects the host to the extent that sialic acid of ductal fibroblast is increased. Number of cytoplasmic process of SD rat bile duct fibroblast was increased. Actin protein and sialic acid were located in cell surface. Fibroblast induced by C. sinensis was not recovered to normal fibroblast. The cytoplasm bulk and cytoplasmic process were increased whereas the growth rate of the fibroblast of infected SD rat was reduced rather than that of normal fibroblast. In result, it inhibits fibroblast proliferation and increases actin protein on fibroblast cytoplasm, and so causes fibroblast metamorphosis and cellular mutation.