• The Korean Journal of Pesticide Science
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• v.14 no.3
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• pp.280-283
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• 2010

Extracts of 33 medicinal herbs belonging to 28 different families used as precious resources in the Northeast Asia were tested for their antiviral activities against two major plant viruses. Twenty one methanol extracts from 19 different families were found to have a antiviral activity against tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) when tested on indicator plants under greenhouse conditions. Most of these extracts have weak activities at the concentration used. But the methanol extracts of Phellinus linteus exhibited potent ($98.7{\pm}1.3%$) antiviral activity against TMV infection and also showed $97.2{\pm}2.2%$ activity against CMV infection. The methanol extracts of the gall of Rhus javanica also showed strong inhibitory efficacy over $98.7{\pm}1.1%$ against TMV or CMV infection. Further research is needed to elucidate the active constituents of these medicinal herbs which may be useful in the development of new and effective antiviral agent against plant viruses.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.76-76
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• 2020

최근 안전한 먹거리 생산과 치유, 교육 등 다양한 목적으로 도시농업에 참여하는 인구가 늘어남에 따라 도시텃밭의 농약, 비료 사용 등으로 인한 환경오염과 해충 피해, 농산물의 건강성 등 다양한 문제가 제기되고 있다. 따라서 도시텃밭 작물의 생육 증진, 잔류농약 문제 해결 등 농산물 안정성 증가에 기여하고자 식물추출물을 활용한 엇갈이 배추의 생육특성을 조사하였다. 선행연구 결과를 바탕으로 자원식물류 5종(쇠비름, 소리쟁이, 명아주, 박주가리, 환삼덩굴)과 허브류 5종(메리골드, 서던우드, 캐모마일, 애플민트, 로즈마리), 식용작물류 5종(양파, 마늘, 부추, 차즈기, 들깨) 등 추출 대상 식물 15종을 60℃에서 3일간 건조시켜 마쇄하였다. 이후 각 식물체 100g을 35% 에틸알코올 1,000ml에서 7일간 추출하였다. 추출물은 30%로 희석하여 주 2회씩 총 3주간 배추에 엽면살포하였다. 엽면적, 엽수, 지상부 및 지하부 생체중 등 생육특성을 7일 간격으로 조사하였으며 그 결과는 다음과 같다. 엽면적과 엽폭은 환삼덩굴 처리구에서 104.58cm²와 9.80cm로 가장 넓었고, 엽장은 박주가리 처리구에서 25.24cm로 가장 길었으며, 지하부 생체중은 메리골드 처리구에서 6.24g으로 가장 높았다. 엽수는 애플민트와 마늘 처리구에서 6.4개로 가장 많았고, 지상부 생체중과 엽록소는 집단 간 유의한 차이가 나타나지 않았다. 전반적으로 무처리 대비 엽면적 22%, 엽폭 18%의 유의한 생장 차이를 보인 환삼덩굴 추출물과 엽면적 12%, 엽장 17%의 생장 차이를 보인 박주가리 추출물, 엽면적 10%, 엽폭 9%의 생장 차이를 보인 양파 추출물이 엇갈이 배추 생육에 효과적인 것으로 나타났다. 본 연구결과 환삼덩굴, 박주가리, 양파 등 일부 식물추출물이 엇갈이 배추의 생육을 증진시킬 수 있는 것으로 나타나 주말농장 등 도시텃밭에 활용할 수 있을 것으로 사료되었다. 향후 식물추출물의 효과성을 증진시키기 위해 추출 방법과 적정 농도, 보관 시기 등에 대한 연구와 함께 도시텃밭 해충방제 효과를 알아보기 위한 약해검정과 살충효과를 분석할 필요성이 있다.

• Research in Plant Disease
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• v.29 no.4
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• pp.459-463
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• 2023

This study was conducted to investigate the plant-parasitic nematodes of ornamental foliage plants, citrus orchards, and tea plantations from July to December 2022. As a result of the investigation of plant-parasitic nematodes in 415 foliage plants, root-lesion nematodes were detected most frequently, followed by root-knot nematodes, pin nematodes, and other nematodes. In tea plantations, spiral nematodes, cyst nematodes, and root-knot nematodes were detected. Citrus nematodes, ring nematodes, and root-knot nematodes were discovered in citrus orchards. In foliage plants, tea plantations, and citrus orchards, the detection rate of plant-parasitic nematodes was not high, but root-lesion nematodes were detected. Therefore, it is necessary to apply appropriate control methods to manage root-lesion nematodes during the cultivation of foliage, tea, and citrus plants.

• Journal of Korean Society of Forest Science
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• v.112 no.3
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• pp.352-362
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• 2023

Harvest time is one of the most important determining factors of seed quality, especially for species that produce seeds over irregular and long-term periods, such as Larix kaempferi. A cone collection plan must be established to increase seed production efficiency and stable mass production. We investigated seed qualities such as seed efficiency, germination rate, and T₅₀ (germination speed), with 7 or 8 cone collection times at a clonal seed orchard of L. kaempferi in Chungju between 2021 and 2022. A multivariate analysis was then performed for the collected data. In early August, decreases in the moisture contents and browning of cones were observed. These were followed by a decrease in germination rate, with a peak at the end of September, but no clear trend was observed. The later the cones were harvested, the better the seed vigor (T₅₀). However, the seed yield and efficiency decreased owing to increases in seed scattering and the number of insect-damaged seeds. As a result, the optimal time of seed harvest for the seed orchard was in early August. To produce uniform seedlings, insect damage must be reduced through timely control and harvest cones in early September. This shows that the degree of browning and moisture content of cones can be used as indicators of the timing of cone collection in L. kaempferi seed orchards.

• Applied Biological Chemistry
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• v.48 no.2
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• pp.150-154
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• 2005

To overcome the problems associated with chemical pesticides, biological pest controls agent extracted from Ginkgo biloba was studied. Insecticidal activities components in Ginkgo biloba extracts were analyzed using high performance liquid chromatography (HPLC). This results of HPLC analysis, GG-W80 were included bilobalide $611\;{\mu}g/kg$, ginkgolide A $37\;{\mu}g/kg$ and ginkgolide B $243\;{\mu}g/kg$, while YG-W80 were included bilobalide $214\;{\mu}g/kg$ and ginkgolide B $46\;{\mu}g/kg$. The biological activity of Ginkgo biloba extracts were conducted to repellent and pesticidial effect of Tetranichus urticae, Aphis gossypii and Myzus persicae treated with Ginkgo biloba leaves extracts. Mortalities of adult T. urticae to green Ginkgo biloba extracts (GG-W80) and yellow Ginkgo biloba extracts (YG-W80) were shown 98.3% and 20.0%, respectively. From these results, terpenes components in Ginkgo biloba extracts could be use for biological controls for T. urticae.

• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.540-549
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• 2016

Plant residues serve as substrates for the proliferation and overwintering of plant pathogenic fungi in soil. Effects of the biocontrol fungus Trichoderma harzianum on the colonization of wheat straw by the plant pathogenic fungus Fusarium solani were investigated under different soil moisture regimes (-50 vs. -500 kPa) in non-sterile soil. T. harzianum ThzID1-M3 and/or F. solani were added along with wheat straw to non-sterile soils. ThzID1-M3, other Trichoderma species, and F. solani were monitored for a 21-day period using quantitative PCR. ThzID1-M3 reduced the colonization of F. solani on wheat straw (p < 0.05) under both moisture regimes, and F. solani reduced the colonization by ThzID1-M3 and other Trichoderma species (p < 0.05), thus suggesting competitive inhibition between ThzID1-M3 and F. solani. Colonization by ThzID1-M3 and generic Trichoderma was improved in the wet soil (p < 0.05), but colonization by F. solani did not differ between the two moisture conditions. Thus, the inhibitory effect of ThzID1-M3 was greater in the wet soil (p < 0.05). The growth competition between ThzID1-M3 and F. solani to colonize plant debris suggests that the biocontrol fungus T. harzianum may reduce the potential of the plant pathogen, F. solani, to survive and proliferate on crops.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.47-55
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• 2016

The purpose of this study was to control root-knot nematode, Meloidogyne incognita on pumpkin seedling with 10%, 20% coffee ground compost and 1% plant velvet bean powder (plain skin bean, leopard skin bean, hole plant, bean pod). Among the root-knot nematode insecticide treatments, the velvet bean plant powder was the highest mortality rate with 83.2% in greenhouse condition. In addition, the root-knot nematode second juvenile mortality significantly increased with the lapse of processing time for each concentration of leopard skin velvet bean extract in vivo. After 24 hours, the mortality rate of root-knot nematode showed the highest at 1.2% and 2.4% of leopard skin velvet bean extract. The growth promoting effect of seedlings pumpkin, 20% of coffee ground compost treatments inhibited the growth of pumpkin seedlings and 1% the tiger pattern velvet beans powder was the most to promote the growth of pumpkin seedlings. However, the results indicated that the organic materials of velvet beans and coffee ground compost are expected to be able to effectively control the root-knot nematode and further studies will be needed for the concentration and application methods.

• Research in Plant Disease
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• v.29 no.2
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• pp.188-192
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• 2023

Abiotic and biotic stresses have been a serious threat to crop growth and productivity in the agricultural system. In this study, four strains (HS1, H30-3, KJ40, and BC42), which have biological activities related to disease suppression or alleviation of salinity and drought stresses, were tested for broad-spectrum biocontrol activity against anthracnose caused by Colletotrichum orbiculare, a bacterial fruit blotch caused by Acidovorax citrulli, and Fusarium wilt caused by Fusarium oxysporum in cucumber plants. As a result of test, when the four strains were drenched into the soil, anthracnose in cucumber leaves significantly decrease; strain KJ40 suppressed disease incidence by A. citrulli; strain BC42 significantly reduced bacterial fruit blotch and Fusarium wilt compared to control. Therefore, strain KJ40 could be a biocontrol candidate for controlling anthracnose through induced systemic resistance and the disease caused by A. citrulli as well as alleviating drought stress; strain BC42 has broad-spectrum biocontrol activity against anthracnose, Fusarium wilt, and bacterial fruit blotch.

• 대한상한금궤의학회지
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• v.14 no.1
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• pp.1-26
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• 2022

Objectives: We aimed to confirm the safety and effectiveness of the decoction of Sibjo-tang, which is a powder type purgative. In addition, we checked whether the '發作' of 『Shanghanlun』 can be interpreted to have the same meaning as "seizure" in English. By confirming these objectives, we intend to lead the progress in the application of Sibjo-tang and to expand the clinical application of the 152nd provision and Sibjo-tang. Methods: We analyzed the medical records of patients who visited Apgujeongjeongin Oriental Clinic and Almyeon Oriental Clinic for seizures. We confirmed side effects in patients who took Sibjo-tang for a long time. Sibjo-tang was prepared as a hot water extract by using 5 g each of Euphorbiae Pekinensis Radix, Eurphobiae Kansui Radix, and Genkwae Flos and 15 g of Zizyphi Fructus. The "seizure" recorded in the 152nd provision was interpreted through the shape analysis of Oracle bone scripts. The seizure frequency of patients diagnosed with epilepsy after taking Sibjo-tang was compared, and we analyzed other symptoms and psychosocial conditions associated with seizures at the first episode. Results: No side effects were found in 7 patients who took Sibjo-tang for 16.57 ± 14.10 months. Seizures were eliminated in 6 cases while taking Sibjo-tang and significantly decreased in 1 case. Frequent indigestion (in 7 cases), mild exhaustion (in 5 cases), and inferiority complex (in 5 cases) were identified as accompanying pathogenic conditions. The "seizure" in the 152nd provision was interpreted as "a state of being stabbed by a sharp substance in clothes", confirming a deep connection with epileptic seizures. Conclusions: The results suggest that using a decoction of Sibjo-tang is safe and efficacious. In addition, the necessity of conducting an advanced study on epileptic seizures accompanied by frequent indigestion using Sibjo-tang was confirmed.

• Research in Plant Disease
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• v.29 no.1
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• pp.23-38
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• 2023

The fire blight caused by Erwinia amylovora (Ea) is a devastating disease of Rosaceae plants, including commercially important apple and pear trees. Since the first report in Korea in May 2015, it has been spreading to neighboring regions gradually. Host plants can be infected by pollinators like bees, rainfall accompanied by wind, and cultural practices such as pruning. Many studies have revealed that wild Rosaceae plants such as Cotoneaster spp., Crataegus spp., Pyracantha spp., Prunus spp., and Sorbus spp. can be reservoirs of Ea in nature. However, wild Rosaceae plants in Korea have not been examined yet whether they are susceptible to fire blight. Therefore, the susceptibility to fire blight was examined with 25 species in 10 genera of wild Rosaceae plants, which were collected during 2020-2022, by artificial inoculation. Bacterial suspension (10⁸ cfu/ml) of Ea type strain TS3128 was inoculated artificially in flowers, leaves, stems, and fruits of each plant species, and development of disease symptoms were monitored. Moreover, the presence of Ea bacteria from inoculated samples were checked by conventional polymerase chain reaction. Total 14 species of wild Rosaceae plants showed disease symptoms of fire blight, and Ea bacteria were detected inside of inoculated plant parts. These results suggest that wild Rosaceae plants growing nearby commercial apple and pear orchards in Korea can be Ea reservoirs, and thus they should be monitored regularly to minimize the damage by Ea infection and spreading.