• Journal of Life Science
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• v.17 no.5 s.85
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• pp.664-670
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• 2007

The propolis is natural product produced by honeybees and is known to have many biologically useful properties such as anti-microbial, anti-oxidative and anti-tumorigenic activity. However, its radio-protective property has not been well studied. To investigate radio-protective effect of propolis on mouse testis, mice were supplemented with propolis after 5 Gy irradiation. The histological changes of testis were detected by TEM. The results indicate that propolis may protect tissue deformation which is induced by 5 Gy of ionizing radiation. Furthermore, to elucidate the potential molecular mechanisms involved in radio-protective property of propolis, we performed microarray experiments using oligo DNA microarray. We found 65 up-regulated genes and 224 down-regulated genes, whose expression levels were affected more than 2-fold by propolis treatment in mice irradiated at 5 Gy. We confirmed microarray data with reverse transcription-PCR using gene specific primers. The results of RT-PCR are highly correlated with those of microarray. These results may help understanding molecular mechanisms of radioprotective effects by propolis in mouse model.

• Journal of Radiation Protection and Research
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• v.20 no.2
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• pp.71-78
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• 1995

To develop methods to reduce radiation risk and apply such knowledge to improvement of radiation protection, the effects of cobaltous chloride known as bioantimutagen on the function of E. coli RecA protein involved in the repair of DNA damage were examined. The results demonstrated two distinct effects of cobaltous chloride on the RecA protein function necessary for the strand exchange reaction. Cobaltous chloride enhanced the ability of RecA protein to displace SSB protein from single-stranded DNA and the duplex DNA-dependent ATPase activity. RecA protein was preferentially bound with UV-irradiated supercoiled DNA as compared with nonirradiated DNA The binding of RecA protein to UV-irradiated supercoiled DNA was enhanced in a dose-dependent manner. It is likely that studies on the factors affecting repair efficiency and the DNA repair proteins may provide information on the repair of ionizing radiation-induced DNA damage and the mechanism for DNA radioprotection.

• The Journal of the Korea Contents Association
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• v.9 no.7
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• pp.211-217
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• 2009

In order to find out the radioprotective effect of algin-oligosaccharide, this study, with a mouse of which whole body irradiated by 3 Gy radiation once, measured nitric oxide. In nitric oxide test for observing the reaction of cell inflammation, nitric oxide showed decreased in the irradiation control group, while 3 day's treatment group with algin-oligosaccharide before or after irradiation indicated higher than the irradiation control group, especially showed big difference in 3 day's treatment group before irradiation (P<0.001). Consequently, this study inquired into the fact that algin-oligosaccharide with superior antioxidant activity performed radiation protection by increasing promotion of nitric oxide generation and confirmed that natural product with less chemical toxicity was able to be applied as radioprotector.

• Journal of Radiation Protection and Research
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• v.22 no.3
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• pp.195-205
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• 1997

This paper is to assess the effect of Adaptagen as a radioprotector in which main component is alkaloid fraction of ginseng. Evaluation was made in vitro and in vivo study with NIGP(S) mouse by the measurement of regeneration of jejunal crypt cell and micronucleus assay to analyze radioprotective effect of ginseng alkaloid fraction in comparison with that of water fraction after whole body irradiation. The results were as follows, 1. The degree of radiation damage of mouse jejunal crypt cell was diminished in both of alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group. Regeneration of mouse jejunal crypt cell was higher both in alkaloid and water fraction groups than control group. 3. In vitro study, frequency of micronucleus was diminished in tendency for the treated groups than control group but statistically insignificant. 4. In vitro study, frequency of micronucleus was diminished in both alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group.

• Journal of radiological science and technology
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• v.33 no.4
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• pp.321-326
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• 2010

To examine the radioprotective effect of algin-oligosaccharide(AOS), radiation-induced IL(interleukin)-6 in mice treated with 3 Gy whole body irradiation once were examined. In the measurement of irradiation-induced IL-6, in comparison with the irradiation control group, in both small intestine and liver tissues of the group treated with algin-oligosaccharide for 7 days prior to irradiation, was suppressed IL-6 synthesis(p < 0.001). It is considered that the protection against radiation hazard by antioxydative reaction of algin-oligosaccharide results in down control of IL-6 value in experimental groups treated with algin-oligosaccharide. In conclusion, through our study, the fact that algin-oligosaccharide has irradiation protection effects was elucidated, and simultaneously, the possibility of the use of a natural product without chemical toxicity as an irradiation protection agent was confirmed.

• 대한방사선방어학회:학술대회논문집
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• 2011.04a
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• pp.72-73
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• 2011

• Journal of radiological science and technology
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• v.27 no.2
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• pp.35-43
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• 2004

Radioprotective effects of ginseng extracts on liver damage induced by high energy x-ray were studied. To one group of ICR male mice were given white(50 mg/kg/day for 7days, orally) and fermenta ginseng extracts(500 mg/kg/day for 7days, orally) before irrdiation. To another group were irradiated by 5 Gy dose of high energy x-ray. Contrast group were given with saline(0.1 ml). This study also investigated the radioprotective effect between SOD, CAT, hydrogen peroxide and ginseng extracts on hepatic damage. This study measured the level of superoxide dismutase(SOD), catalase(CAT), hydrogen peroxide($H_2O_2$) in liver tissue. Administrating orally white (50 mg/kg/day for 7days, orally) and fermenta ginseng extracts(500 mg/kg/day), the activity of SOD, CAT were generally increased and the hydrogen peroxide($H_2O_2$) was decreased. After irradiation, the activity of SOD, CAT were generally decreased and the hydrogen peroxide($H_2O_2$) was increased. Therefore, ginseng extracts increased antioxidative enzyme activity. And We know that the antioxidatant effect of extracts from white and fermenta ginseng protect radiation damage by direct antioxidant effect involving SOD, CAT. It was included that ginseng can protect against radiation damage through its antioxidatant properties.

• Journal of radiological science and technology
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• v.41 no.5
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• pp.471-478
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• 2018

Oogenesis process of ovary produces a lot of undifferentiated cells. Especially, the radiation exposure of early immature cells in the process of growth to oocyte causes serious disabilities. This study examined the radiation damage mechanism of undifferentiated cells and organelles in oogenesis process, and the radioprotection after injection of alliin. The ultrastructure after 7Gy X-ray irradiation on the white rat was observed in the experiment. The results is as follows. It was observed that the nucleus membrane of an oogonium was damaged and vacuolated in the several parts after 15 days of irradiation. The damage of mitochondria membrane and flow in cytoplasm after 20 and 30 days was found in the oogonium. After 40 days observation, peroxidation of fat droplets was found and organelles were tangled each other in ovary tissue. The partial damage of nuclear membrane in oogonium past 15 days after injection of alliin was found, but decreased remarkably. Mitochondria, Golgi body, and rough endoplasmic reticulum were also clearly observed, therefore, radioprotection effects in alliin was confirmed partially.

• The Journal of the Korea Contents Association
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• v.16 no.10
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• pp.563-572
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• 2016

The purpose of this study was to examine the knowledge, attitude and educational needs of radiation protection among nursing students and nurses, because it is needed to raise awareness of the importance of radiation protection. The participants in this study were 274 including 135 registered nurses on bachelor's degree course who had experience of the operating room practice and 139 nursing students. The questionnaire consisted of 45 items including knowledge, attitude, and educational needs of radiation protection. The findings of the study suggest that safe management education was insufficient for protection of radiation. Though, educational needs and intention of involvement were high, the respondents who were more knowledgeable about radiation protection took a better attitude of radiation protection. A radiation protection course development is needed according to educational needs. Nurses have to get a chance to receive education outside the hospital or be provided with a radiation protection program as internal education. Also, radiation educational experts and various visual and auditory materials are needed to promote effectiveness of education.

• 대한방사선방어학회:학술대회논문집
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• 2011.11a
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• pp.138-139
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• 2011