• 한국수정란이식학회지
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• 제20권1호
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• pp.1-8
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• 2005

본 실험은 LEY에 다양한 당류를 첨가한 용액으로 동결보존한 돼지정자의 응해후 생존율과 정상 첨체율, 체외성숙한 난자와의 수정능력과 배 발달 능력 및 인공수정을 통한 수태율과 산자수에 미치는 효과를 조사하기 위해 실시하였다. 융해후의 정상 첨체율은 glucose 농도 증가와 함께 증가되었으나, 생존율에 있어서는 효과가 인정되지 않았다. LEY에 fructose를 단독 또는 glucose와 함께 첨가하면 융해후의 생존율이 유의하게 증가되었으며 (p<0.05), 정상 첨체율에 있어서는 LEY 보존액에 fructose와 glucose를 첨가한 구가 $81.4{\pm}2.3\%$로 control의 $41.6{\pm}0.6\%$에 비하여 유의하게 높았다(p<0.001). 체외성숙 난자와의 수정율, 분할율 및 배반포 발생율은 $70.8\~80.7\%$, $44.6\~45.7\%$ 및 $13.6\~16.0\%$로 glycerol과 fructose 농도 및 정자농도 간에 차이가 인정되지 않았다. 1회 발정당 2회의 인공수정을 하였을 때, 수태율은 $83.3{\pm}0.1\%$, 산자수는 $9.4{\pm}1.7\~10.4{\pm}0.7$두로서 SGI사의 동결정액의 $50.0{\pm}0.1\%(p<0.05)$과 $8.0{\pm}1.1$두에 비하여 높았다.

• 한국수정란이식학회지
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• 제25권3호
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• pp.133-139
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• 2010

The purpose of this study was to determine the relationship between conception rate and other parameters (body condition score; BCS, progesterone concentrations and follicle size) before estrus induction with $PGF_2{\alpha}$. The conception rate in cows with (2.75, 2.75 to 3.25 and 3.25), BCS regardless of AI (artificial insemination) time was 47.5, 67.5% and 48.5% at $PGF_2{\alpha}$ injection, respectively. The conception rate regardless of BCS was 59.0% in cows inseminated based on detected estrus, and 46.2% in cows inseminated at 72 to 80 hours (timed artificial insemination, TAI) after $PGF_2{\alpha}$ injection. The conception rate regardless of AI time was 43.0% in cows with low progesterone concentrations (less than 1.0 ng/ml), and 67.5% in cows with high progesterone concentrations (more than 1.0 ng/ml) at $PGF_2{\alpha}$ injection. The conception rate regardless of progesterone concentrations was 59.9% in cows inseminated based on detected estrus, and 48.1% in cows of TAI after $PGF_2{\alpha}$ injection. The conception rate regardless of AI time was 36.0% in cows with small dominant follicles (less than 5 mm), 56.0% in cows between 5 mm to 10 mm of follicle size, and 65.5% in cows with large dominant follicles (more than 10 mm) at $PGF_2{\alpha}$ injection, respectively. The conception rate regardless of follicle size was 57.3% in cows inseminated based on detected estrus, and 47.6% in cows of TAI after $PGF_2{\alpha}$ injection. These results indicated that if the cows with BCS 2.75 to 3.25, active corpus luteum, and/or large dominant follicle (more than 10 mm) are used for estrus induction, the conception rate will be greater.

• 한국수정란이식학회지
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• 제25권1호
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• pp.67-75
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• 2010

To investigate the change of vaginal epithelial cell in estrus-induced Shih-tzu bitch, estrus was induced by PMSG (50 IU/kg, for 10 days) and hCG (1,000 IU, on Day 10) in 12 anestrus Shih-tzu bitches. Day-changes of vaginal epithelial cells during the whole period of hormone treatment were investigated in each experimental bitches. The first day of vulvar bleeding and the first day of male acceptant was on $6.72{\pm}1.38$ days ($Mean{\pm}S.D.$), and $10.44{\pm}1.63$ days after the first PMSG treatment. The duration of proestrus and estrus was $4.36{\pm}1.20$ days, and $7.58{\pm}1.43$ days. Characteristic features of vaginal cytology during the estrous cycle were the high proportion of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell and anuclear cell in estrus, and parabasal cell, small and large intermediate cell, and leukocyte in diestrus, respectively. When it was timed from the first day of PMSG administration (Day 0), the cornification index (CI) was the high proportion in proestrus and estrus. The CI peak was maintained above 80% between Day 11~14 (4 days) and CI showed a peak at Day 12. These results indicated that the 12 Shih-tzu bitches showed positive estrus induction by vaginal smear test and observation of clinical estrus sign.

• 대한수의학회지
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• 제29권3호
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• pp.373-381
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• 1989

The aim of the present study was to test the efficiency of estrous induction in the premature, metestrous and anestrous bitches. The estrus was induced with prostaglandin $F_{2{\alpha}}$, estradiol-$17{\beta}$, pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin(HCG) in the treatment A, and with PMSG and HCG in the treatment B. Day 0 was the first day of estrone injection in the treatment A and the day of PMSG injection in the treatment B. Twenty three of the twenty six bitches were laparotomized under general anesthesia between 11 and 18 days after onset of behavioral estrus, whereas three bitches were not laparotomized and remained until parturition. Ovarian responses were evaluated with the total number of corpora lutea or ovulation sites. The uterine horns were flushed with phosphate-buffered saline added heat treated canine serum(10%), the flushing media was collected into watch glass and the ova were examined under stereomicroscope. The results obtained were as follows: 1. Standing estrus was observed on the day $17.7{\pm}1.5$ after injection of estrone in the treament A, but ovarian responses were not detectable. 2. Standing estrus was observed on the day $12.2{\pm}0.2$ after injection of PMSG in the treament Band 14 of 17 bitches showed ovarian responses. Ova were recovered in 9 of the 14 bitches. 3. Ovarian responses were observed in one of the three premature bitches. two of the three metestrous bitches and all of the 11 anestrous bitches. The average number of the ova collected from 9 bitchs were $12.2{\pm}1.4$. 4. Three bitches in the treament B exhibited behavioural estrus and all of them were mated with fertile male dog, resulting the pregnancy in only one bitch. The pregnant bitch gave the birth of two pups.

• 한국수정란이식학회지
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• 제22권2호
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• pp.111-114
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• 2007

본 연구는 젖소의 산유 능력에 따른 번식 성적을 조사하기 위하여 축산과학원 시험우사에서 사육중인 젖소를 이용하여 분만 후 유즙중 P4농도를 분석하였고, 전문경영체농장 22개 소에서 사육중인 젖소에 대한 번식 기록을 이용하여 고능력우(305일 유량 10,000 kg 이상)와 평균 능력우(305일 유량 10,000 kg이하)의 번식 성적을 조사한 결과는 다음과 같다. 1. 젖소의 분만 후 progesterone peak는 고능력 및 평균 능력우의 경우에 각각 $38.8{\pm}11.1$ 및 $39.6{\pm}9.7$일로 거의 비슷한 결과를 나타내었다. 2. 분만 후 첫 발정까지의 일수는 평균 능력우 및 고능력우가 각각 $99.4{\pm}2.1$일 및 $117.7{\pm}4.2$일로 고능력우가 평균 능력우에 비하여 11.6일 늦어지는 결과를 나타내었고(p<0.01), 분만 후 수태까지의 일수도 평균 능력우 및 고능력우가 각각 $145.9{\pm}3.8$일 및 $165.9{\pm}6.5$일로 고능력우가 평균 능력우에 비하여 20일 늦어지는 결과를 나타내었다(p<0.01). 그리고 분만 간격은 평균 능력우 및 고능력 우가 각각 $421.5{\pm}4.0$일 및 $448.4{\pm}7.2$일로 고능력우가 평균 능력우에 비하여 26.9일 연장되었다(p<0.01).

• 한국수정란이식학회지
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• 제17권1호
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• pp.33-44
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• 2002

본 연구는 기존의 수정란 이식기술을 다각적으로 분석하고 개선하여 한우 체내수정란의 생산 및 이식기술 체계를 확립하기 위하여 수행하였다 수정란의 생산 및 이식은 농협중앙회 가축개량사업소 한우개량부에서 사육하고 있는 공란우 232두와 수란우 434두를 이용하여 실시하였다. 본 연구에서 얻어진 결과를 요약하면 다음과 같다. 1. 수란우의 황체 위치와 등급에 따라 수정란이식을 실시한 결과는 오른쪽 난소의 A등급 황체, 왼쪽 난소의 B등급 황체가 존재할 경우가 유의적(P<0.1)으로 높은 수태율을 나타냈다. 2. 동결수정란을 이식했을 때의 수태율을 35.0% 로서 신선수정란의 수태율 56.2%에 비하여 유의(P<0.01)하게 낮았다. 3. 수란우의 발정일차(6.0∼9.0일)에 따라 수정란을 이식한 격과는 신선수정란에서 45.4∼65.7%, 동결수정란에서 22.0∼50.0%의 임신율을 나타내서 처리간에 유의성이 인정되지 않았다. 4. 수란우의 황체등급과 수정란의 동결 여부에 따른 임신율을 신선 및 동결수정란 모두에서 A등급의 황체일 경우가 40.8∼67.9%로 B 및 C등급 황체의 25.0∼56.0%보다 양호할 성적을 나타냈다. 5. 수정란의 발육단계 및 등급에 따라서 이식한 결과는 상실배의 이식이 수정란의 등급에 관계없이 평균 46.3%의 임신율을 나타내 어서 배반포배 이식의 34.1%보다 높게 나타났다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.59-59
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• 2003

재래산양은 우리나라 고유의 유전자원으로서 보존뿐만 아니라 개량이 절실히 요구됨에도 불구하고 첨단생명공학기술을 이용한 연구가 극히 미진한 실정이다. 본 연구는 재래산양에 있어서 과배란처리에 의한 미수정 난자의 회수율 개선과 최적의 quality를 확보하기 위하여 과배란 처리 후 회수시간이 난포의 발달과 난자의 회수율에 미치는 영향을 조사하였다. 공시동물은 체중 15kg 전후의 성숙한 미경산 재래산양으로서 발정동기화를 위하여 CIDR(Eazi Breed, InterAg, New Zealand)를 10일간 질내에 삽입하고 과배란 처리는 FSH를 CIDR 삽입 8, 9, 10일째에 12시간 간격으로 70mg을 감량법으로 투여하였으며, PGF2$\alpha$는 8일째에 FSH와 함께 투여하고 CIDR제거는 10일째에 제거와 동시에 hCG 400IU를 투여하였다. 수정란회수는 외과적인 방법으로 실시하였으며, 난관에서 관류방법으로 난자를 회수한 다음 난포에서 흡입방법으로 난포란을 회수하였다. hCG 투여 후 29~34시간과 46~50시간에 관찰된 난포수는 두당 10.29개(144/14) 및 7.83개(47/6)였다. 또한 난포로부터 난포란의 채란율은 각각 66.0%(95/144) 및 53.2%(25/47)였으며, 두당 6.79$\pm$0.9개 및 4.17$\pm$1.1개의 난자를 채란하였다. hCG 투여 후 회수시간에 따른 난관에서 회수한 난자 수는 29~34시간 및 46~50시간 후 채란시 배란점은 평균 4.36개(61/14) 및 7.33개 (44/6)였으며, 난관에서 난자 채란율은 39.3%(24/61) 및 29.5%(13/44)였으며, 재래산양 한마리당 평균 1.71$\pm$0.5개 및 2.17$\pm$1.4개의 난자를 채란하였다. 난관으로부터 회수한 난자의 회수율은 29~34시간째에는 61개의 배란점을 확인하였으나 회수한 난자수는 24개로서 39.3%의 회수율을 보였고 두당 회수율은 1.71$\pm$0.5개였으며, 46~50시간에서는 29.5%의 회수율과 2.17$\pm$1.4개의 두당 회수율을 보였다. 회수한 난포란의 등급에 있어서 29~34시간에 등급별 회수율이 각각 GI(27.4%), GII(14.7%), GIII(43.2%), GIV(14.7%)로 나타났으며, 46~50시간에는 GI(12.0%), GII(12.0%), GIII(28.0%), GIV(48.0%)로 나타났다. 이상의 결과로 볼 때 미성숙 난자의 회수는 hCG 투여 후 29~34시간이 적합한 것으로 생각된다.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.349-353
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• 2011

The objective of this study was to investigate the relationship between estrous expression, body condition score (BCS), blood urea nitrogen (BUN) and number of transferable embryos for the purpose of improving reproductive performance in blood of Hanwoo donors. Sixty, at random stages of the estrous cycle, received a CIDR. Four days later, the animals were superovulated with a total of 28AU FSH (Antorin, 2AU=1 ml) administered twice daily in constant doses over 4 days. On the 3th administration of FSH, CIDR was withdrawn and 25 mg PGF2 ${\alpha}$ was administered. Cows were artificially inseminated twice after estrous detection at 12 hr intervals. The cows received 100 ${\mu}g$ GnRH at the time of 1nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. The estrous inducement rate and estrous expression rate were significantly lower for cows with BCS below 2.25 than for cows with BCS above 2.25. There was 50.0% of rate of mounting in cows with BCS below 2.25 whereas the rate of mounting was markedly increased in cows with BCS above 2.25 (94.1% and 89.5% for BCS 2.25~2.75 and BCS above 2.75 cows, respectively). Cows with BCS <2.25, 2.25~2.75 and ${\geq}$2.75 had number of transferable embryos of $4.5{\pm}0.7$, $5.9{\pm}1.8$ and $5.6{\pm}2.3$ respectively.

• Reproductive and Developmental Biology
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• 제36권3호
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• pp.213-217
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• 2012

The decreased fertility is frequently thought to be problem of cattle production. However, studies figure out that number of these problems is related to bull factors especially in artificial insemination setting. Therefore, this study was designed to investigate the fertility status of bull by their estimated relative conception rate of cows that were inseminated by frozen semen from Korean proven bulls. Here we use the non-return rate (NRR) to access the bull fertility whereas, the NRR was define as the proportion of bulls that semen were used to inseminate cows and the number of cows that did not return for another service within 60 days. The data from 54,388 artificial inseminations (AI) were analyzed from 88 KPN semen. The NRRs of highest and lowest fertile bull were 83.81 and 51.33%, respectively. And mean NRR was 68.27%. In comparison to previously reported study, our data shows 17.38% higher NRR and the absolute value of difference in 50%>NRR and 50%

• Reproductive and Developmental Biology
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• 제36권3호
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• pp.219-223
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• 2012

The present study was performed to identify the relationship between plasminogen activator (PA) and Heat Shock Protein-90 (HSP-90) in porcine uterus tissues during the estrous cycle. Porcine uterus tissues were obtained from preovulatory (Pre-Ov), post-ovulatory (Post-Ov) and early to mid-luteal (Early-mid L) stages. The protein was extracted from uterus tissue by using M-PER Mammalian Protein Extraction Reagent. Proteins were refined by RIPA Buffer and quantified by BCA methods. As results, t-PA expression was significantly (p<0.05) higher from pre-ovulatory(Epithelium tissue: $29,067{\mu}g/{\mu}l$, Myometrium tissue: $30,797{\mu}g/{\mu}l$) compared to the post-ovulatory stage(Epithelium tissue: $54,357{\mu}g/{\mu}l$, Myometrium tissue: $53,270{\mu}g/{\mu}l$) and early to mid-luteal stage(Epithelium tissue: $42,380{\mu}g/{\mu}l$, Myometrium tissue: $43,139{\mu}g/{\mu}l$). On the other hand, the uPA expression indicated higher from early to mid-luteal stage (Epithelium tissue: $0.02198{\mu}g/{\mu}l$, Myometrium tissue: $0.02412{\mu}g/{\mu}l$) than pre-ovulatory stage (Epithelium tissue: $0.01577{\mu}g/{\mu}l$, Myometrium tissue: $0.01531{\mu}g/{\mu}l$) and post-ovulatory stage(Epithelium tissue: $0.01414{\mu}g/{\mu}l$, Myometrium tissue: $0.01429{\mu}g/{\mu}l$). However, expression of u-PA did not differ from each estrous cycle in the epithelium tissue and myometrium tissue(p<0.05). Expression of HSP-90 was differ t-PA and u-PA from pre-ovulatory in Epithelium tissue($25,423{\mu}g/{\mu}l$) and early to mid-luteal stage in epithelium tissue($177,922{\mu}g/{\mu}l$) and myometrium tissue($26,664{\mu}g/{\mu}l$). These results suggest that HSP-90 and u-PA were related with change of uterus cycle according to the reformation of the tissues in porcine uterus.