• 한국임상수의학회지
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• 제29권4호
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• pp.348-351
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• 2012

질을 경유한 반복된 인공수정 실패의 병력을 가진 17개월령 홀스타인 미경산우에서, 질경 검사로 바깥요도구멍 후방에 무공질판막의 존재를 확인하였으며, 이것은 질벽과 비슷한 외관을 보여주었다. 초음파검사 결과 질판막과 자궁경 사이 질강 내에 저에코성 액체의 존재, 즉 질수증의 동반을 확인하였다. 미경산우를 진정 및 경막외마취 후, 수술용 칼과 가위로 무공질판막을 절개 및 제거하였으며, 절개 부위는 단순결절봉합 하였다. 미경산우는 약 20일 간격의 3회 연속 발정에 따른 인공수정 후 임신되었으며, 수정일로부터 291일 경과 후 건강한 수컷 송아지를 순산하였다. 본 증례는 홀스타인 미경산우에서 질수증을 동반한 무공질판막의 발생과 질판막의 제거 수술 후 성공적인 인공수정, 임신 및 분만을 보고한다.

• 한국임상수의학회지
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• 제28권2호
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• pp.267-270
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• 2011

소에서의 난소종양은 흔치 않으며 발견되는 대부분의 난소종양은 과립막세포종양이다. 소의 난소섬유종은 매우 드물며 노령의 동물에서 발병하는 경향이 있다. 본 증례에서는 분만 후 발정정후를 나타내지 않은 초산 젖소에서 발견된 큰 난소종괴를 연속적으로 관찰한 결과이다. 종괴를 초음파로 6개월 동안 연속적으로 관찰한 결과 크기가 지속적으로 증가하였다. 난소절제술을 시행하여 육안검사 및 조직학적 검사소견으로 난소섬유종으로 진단하였다. 본 증례는 살아있는 젖소에서 연속적인 관찰과 진단을 내린 난소섬유종의 첫 사례이다.

• 한국임상수의학회지
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• 제26권5호
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• pp.486-489
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• 2009

An 8-year-old Thoroughbred mare showing anestrus for longer than 6 months with left ovarian mass was referred to Veterinary Medical Teaching Hospital of Seoul National University. For 6 months, the mare had received 2 times hormonal therapies with $PGF_2{\alpha}$ and progesterone. The mare had not responded to these therapies and not shown estrous behavior. On rectal palpation, the left ovary was enlarged and contralateral ovary was small and inactive. Transrectal ultrasonographic examination of the left ovary revealed a mass composed of multiple, irregularly shaped follicles. Granulosa cell tumor (GCT) was suspected according to her history. Ovariectomy was performed through the left flank laparotomy with a standing position in a simple chute located outside of horse farm. The mass was diagnosed as GCT on histopathology. The mare was recovered without any problem and became pregnant next spring.

• 한국임상수의학회지
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• 제30권4호
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• pp.305-309
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• 2013

개에서 자궁축농증은 발정휴지기에 주로 발생하는 질병으로서 급성으로 발병하여 만성 질병 경과를 나타낸다. 또한 장막성 낭포는 분만 후 자궁의 퇴축이 급속하게 일어나는 수복기 동안 흔히 발생한다. 10세 암컷 골든 리트리버견이 무기력, 식욕부진, 빈호흡, 복부팽만 및 비정상적인 외음부 분비물의 증상으로 내원하였다. 방사선 검사, 초음파 검사 및 실험실적 검사를 통하여 자궁축농증으로 진단되었고 난소자궁적출술이 시행되었다. 육안으로 관찰한 결과, 자궁은 팽만 되어 있었으며, 부분적으로 복벽과 유착되어있었다. 또한, 자궁 전반에 걸쳐 맑은 액체로 충만해 있는 낭포들이 관찰되었다. 세균배양검사를 통하여 자궁내강에 차있던 농에서 대장균이 감염되어있다는 것을 확인하였으며, enrofloxacin에 감수성이 있었다. 낭포에 대한 조직검사를 실시하여 자궁축농증과 장막성 낭포로 최종 진단하였다.

• 한국수정란이식학회지
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• 제13권3호
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• pp.213-217
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• 1998

본 연구는 한우 다배란처리시 노동력을 절감하고 공란우의 스트레스를 방지하여 정상수정란을 안정적으로 생산할 수 있는 1회 주사시 FSH의 용해제의 종류와 농도를 선정하기 위하여 다양한 용매제에 용해시킨 1회 주사와 기존의 다회주사에 의한 난소반응을 비교 검토하였다. 1. 공란우에 다배란 처리후 발정유기는 처리 1과 7에서 100% 유기되었다. 2. 채란수와 정상수정란 수는 처리 1과 처리 7에서 (3.5, 2.9와 3.8, 3.5) 다른 처리구에 비해 유의하게 많았다(p〈0.05). 3. FSH 주사후 7일째 혈중 progesterone농도 역시 처리 1과 처리 7에서(6.19ng/ml, 7.54ng/ml) 다른 처리구에 비해 유의하게 높았다(p〈0.05).

• 한국동물위생학회지
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• 제26권2호
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• pp.151-156
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• 2003

This study was carried out to get a fundamental information for improvement of reproductive performance in gilt. We investigated the effects of breeds on body weight, age, body length, wither's height, girth and backfat thickness, and the serum concentrations of estradiol-17${\beta}$, cortisol and progesterone at first estrus and mating of gilts. A total of 47 gilts of Duroc, Landrace and Yorkshire, produced at Livestock Experiment Station, Chungnam livestock sanitation research institute from 2000 through 2002, were used for this experiment. Body weight, age and girth of Duroc at frist estrus and mating were higher than those of Landrace and Yorkshire. There were no differences on body length among the three breeds at frist and mating. Wither's height of Duroc and Yorkshire at first estrus and mating was higher than that of Landrace. Backfat thickness of Yorkshire was thinnest among the three breeds at first estrus, but there were no differences on backfat thickness among the three breeds at first mating. Serum estradiol-17${\beta}$ concentration of Landrace(45.0 pg/ml) at first estrus was higher than that of Yorkshire(27.4 pg/ml) and Duroc(21.8 pg/ml), but there were no differences on estradiol-17${\beta}$ concentration (from 18.5 to 31.9 pg/ml) among the three breeds at first mating. Serum cortisol concentration of Duroc at first esturs and mating was higher than that of Landrace and Yorkshire. There were no differences on serum progesterone concentration among the three breeds at first estrus and mating of gilt.

• Applied Microscopy
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• 제25권1호
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• pp.75-85
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• 1995

The goal of the present study is to investigate the precise variation of tubulin substances in the cytoplasm of oviductal ciliated cells and the morphological changes in cytoplasmic organelles of ciliated cells for ciliogenesis by estrous cycles. The animals used in this study were female rat (Sprague Dawley strain), weighing approximately 200 gm. The ampulla oviducts of these animals (at each of estrous cycle) were rapidly excised. At each stage of estrous cycle, the tissues were used for immunocytochemical study and other were used for electron microscopical study. All specimens were observed by the light and electron microscope. The results obtained are as follows: 1. In the ciliated cells at proestrus, Golgi complex showed $5{\sim}7$ stacked cisternae with numerous saccules and vacuoles. Large amount of fibrous granules were located near the Golgi complex. But at metestrus and diestrus, few fibrous granules were seen. 2. A moderate number of rough endoplasmic reticulum and polyribosomes were scattered in the cytoplasm of ciliated cells at proestrus, but were decrease in number at metestrus and diestrus. 3. At proestrus and estrus, there were a large amount of vesicles in the apical cytoplasm of ciliated cells. 4. Numerous mitochondria were located in the apical cytoplasm at proestrus and estrus, but only a few at metestrus and diestrus. 5. At proestrus and estrus, tubulin substances showed strong reactions in the cytoplasm but weak reactions at metestrus and diestrus. It is suggested consequently that the ciliated cells of the rat oviducts showed no morphological changes of cilia but the ultrastructural organelles of the cells were changed in its shape and location during the entire estrous cycle.

• 한국수정란이식학회지
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• 제6권2호
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• pp.37-46
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• 1991

A field trial was performed to evaluate the effects of hormone treatment on estrus induction, ovulation, embryo transfer and reproductive performance in post-weaning sows. This trial involved 61 mixed breed sows of varying parity on a commercial pig farm. Sows were allocated to one of five trials: control group involved 25 sows that were treated with a single intramuscular injection of 5 ml physiological saline, 6 sows received 1,500 IU PMSG on the day of weanning and 500 IU HCG at the onset of estrus in trial I, 7 sows received 750 IU PMSG on the day of weanning and 500 IU HCG at the onset of estrus in trial II, 5 sows were treated with the same as trial II on day 28 after weanning in trial III. and 18 sows were treated with 10 mg PGF$_2$$\alpha$ plus 2 mg estradiol benzoate on day 31 after weanning in trial IV. Ovarian responses were checked by laparotomy and ova were recovered by oviducal flushing between 40 and l00hrs after mating. Fertilized ova were transferred into the oviduts of recipient sows synchroni- zed. The results obtained were summarized as follows: 1. Percentages of sows detected in standing estrus following treatment were 86~100% among trial groups. The interval from treatment to standing estrus(6l.7$\pm$0.5lhrs) in lOmg PGF$_2$$\alpha$ and 2mg estradial henzoate treated group was significantly earlier than in other trial groups(P<0.05). 2. Average number of ovulations was 11.5~37.8 among trial groups. The ovulation rate in 1,500 IU PMSG and 500 IU RCG treated group (37.8$\pm$ 19.87) was significantly different from other trial groups(P<0.05). 3. Ova were recovered by oviducal flushing between 40~ l00hrs after mating and recovery rates of ova wore 91.4% between 40~59hrs. 4. Fertilized ova were transferred into the oviducts of 8 recipient sows synchronized with 7 to 17 ova per animal. Three of the recipients were pregnant and delivered 25 piglets. 5. Four of the donor sows in those embryo collection was not successful were pregnant following oviducal flushing and delivered 23 piglets. 6. Recurrence of estrus and farrowing performance of experimental sows were observed following the experiment was no difference among trial groups, respectively.

• Reproductive and Developmental Biology
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• 제30권1호
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• pp.7-11
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• 2006

본 연구는 호르몬과 Na-pyruvate의 첨가가 개 미성숙난자의 체외성숙에 미치는 영향을 검토하였다. 개의 발정주기와 관계없이 암캐의 난소에서 회수한 난자를 NCSU-37 배양액에서 72시간 체외 배양하였다. 호르몬의 영향을 검토하기 위하여 배양액 중에 다양한 호르몬을 $24{\sim}72$시간 첨가하여 성숙율을 검사하였으며, Na-pyruvate 첨가 농도의 영향을 검토하였다. $E_2$ 단독 첨가구에서는 제2감수분열 중기(MII) 단계까지 성숙이 관찰되었으나(5.7%), 타 처리구에서는 MII기까지의 성숙이 이루어지지 않았다(P<0.05), $E_2$를 $6{\sim}24$시간만 처리하였을 때는 MII기로 성숙되는 난자가 없었으나 72시간 처리하였을 때는 6.0%로 유의적으로 높게 나타났다(P<0.05). Na-pyruvate의 농도를 0.25 mM 첨가한 구보다 2.5 mM 첨가한 구에서 제1감수분열 중기(MI) 단계까지의 성숙율이 증가하는 경향을 보였으나, MII 단계까지의 성숙율은 차이가 없었다. 본 실험의 결과는 개 난자의 체외성숙시 $E_2$가 첨가된 배양액에서 72시간 배양시에 성숙율을 증진시킬 수 있으나, Na-pyruvate의 농도는 개 체외배양에 있어 큰 영향을 미치지 않는 것을 나타낸다.

• 한국임상수의학회지
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• 제20권3호
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• pp.369-375
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• 2003

The ultrasonography was performed in 77,924 subestrous dairy cows from 680 dairy farms at 57 districts. The cows were examined for confirmation of resumption of ovaries and uterus after 30 days postpartum, for early pregnancy diagnosis after 30 days post-insemination, confirmation of pregnancy at 8 month post-insemination, and for diagnosis of reproductive dysfunctions, respectively. The results of reproductive examination by ultrasonography in 77,924 dairy cows were ovarian disorders 35,452 (46.8%), uterine disorders 10,721 (13.8%), pregnancy and pregnant failure 24,044 (30.8%), adhesion of reproductive tracts 167 (0.2%), freematins 13 (0.01%) and others 6,527 (8.4%) cows, respectively. The 13,732 (49.6%) cows out of 27,694 cows were treated for silent heat or error of estrus detection, 3,473 (12.5%) cows for follicular cysts, 815 (3.0%) cows for luteal cysts, 606 (2.2%) cows for inactive ovaries, 4 (0.01%) cows for granulosa cell tumors, and 6,455 (23.3%) cows for endometritis, respectively. The indices of reproductive efficiencies before (2001-08-10) and after (2003-07-20) periodical examination of reproductive status were as follows; the mean intercalving interval were shortened from 471 days to 383 days, the mean interval calving to conception were shortened from 187 days to 99 days, the mean interval calving to first service were shortened from 105 days to 67 days, the mean cows showing heat by 60 days postpartum were increased from 33% to 89%, the mean conception rate to first service were increased from 43% to 68%, and the mean service per conception were shortened from 2.6 times to 1.8 times, respectively. These results suggest that periodical examination of reproductive status would be useful for the improvement of the reproductive performance in dairy cows.