• Journal of Korean Ophthalmic Optics Society
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• v.20 no.3
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• pp.391-396
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• 2015

Purpose: The objective of this study was to investigate the visual system of the greater horseshoe bat (Rhinolophus ferrumequinum) by location analysis of some major neurotransmitters glutamate, ${\gamma}$-aminobutyric acid (GABA), acetylcholine, and their receptors, and $m{\ddot{u}}ller$ glial cells in retina. Methods: Standard immunocytochemical techniques were used after vibratome section of retinal tissues of adult greater horseshoe bat for this study. Immnoreactions in immunofluorescence images were analyzed using confocal microscope. Results: Anti-glutamate-immunoreactive neurons were mainly localized in the ganglion cell layer (GCL). The majority of anti-GABA-immunoreactive cells distributed in the inner nuclear layer (INL), and GABAA receptors were localized in the inner plexiform layer (IPL). Anti-choline acetyltransferase-immuoreactive cholinergic neurons were mainly located in the INL and GCL, and most of nicotinic acetylcholine receptors were localized in the IPL. The $m{\ddot{u}}ller$ cells in the retina of the greater horseshoe bat stretched theirs range from the GCL to outer nuclear layer (ONL). Conclusions: This study revealed that the retinas of the greater horseshoe bats contain the same major neurotransmitters and receptors, and glial cell in visually functional mammalian retinas. The present results may suggest that the greater horseshoe bats have the functional retinas for visual analysis through the organized retinal neural circuits.

• Journal of Environmental Impact Assessment
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• v.31 no.5
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• pp.310-320
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• 2022

This study aimed to identify the change in the public perception of "bats" after the outbreak of the coronavirus (COVID-19) infection. Text mining and network analysis were conducted for blog posts, the largest social network in Korea. We collected 9,241 Naver blog posts from 2019 to 2020 just before the outbreak of COVID-19 in Korea. The data were analyzed with Python and NetMiner 4.3.2, and the public's perception of bats was examined through the relationship of keywords by period. Findings indicated that the frequency of bat keywords in 2020 increased more than 25 times compared to 2019, and the centrality value increased more than three times. The perception of bats changed before and after the outbreak of the pandemic. Prior to COVID-19, bats were highly recognized as a species of wildlife while in the first half of 2020, they were strongly considered as a threat to human society in relation to infectious diseases and health. In the second half of 2020, it was confirmed that the area of interest in bats expanded as the proportion of ecological and cultural types ofresearch increased. This study seeks to contribute to the expansion and direction of future research in bats by understanding the public's interest in the potential impact of the species as disease hosts post the COVID-19 pandemic.

• The Korean Journal of Zoology
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• v.32 no.4
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• pp.374-392
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• 1989

한국산 박쥐류의 계통분류학적 연구의 첫 단계로써, 1985년 10월부터 1987년 7월까지 18장소에서 채집된 한국산 박쥐류 중, 관박쥐과(Rhinolophidae)의 1종과 애기박쥐과 (Vespertilionidae)의 6종에 대하여 외·내부형태 및 상환골의 형태를 이용하여 분류학적인 검토를 하였다. 이들 중에는, 희귀종인 문둥이 박쥐(Eptesicus serotinus brachydigitus)가 포함되어, 본 아종에 대한 상세한 형태학적인 특징을 추가·기재하였다. 또한 현생 한국산 익수류상, 고기후 및 고지리를 고려하여 한국산 소익수류상의 성립과정에 대하여 고찰하였다. 즉, 홍적세의 고기후 및 고지리의 변화에 의해서, 남방계보다 북방계 종류가 풍부한 현재의 익수류상이 성립되었다고 생각된다. As a part of studies on taxonomy and phylogeny of bats inhabiting Korea, a taxonomic review of one rhinolophid and six vespertilionid bats collected at 18 localities during the period from October 1985 to July 1987 was carried out, basing on the external, cranial, dental and humeral morphology. These bats include a rare species, Eptesicus serotinus brachydigitus, the detailed characters of which were described in addition to the original description by Mori (1928b). Further, the Korean microchiropteran faunal succession was described laking into consideration the recent chiropteran fauna, and climatic and geographical changes in the Pleistocene: the recent bat fauna of Korea has become abundant in the north-originating species rather than in the south-originating ones, which is a reverse tendency in the Palearctic continent.

• Journal of Korean Ophthalmic Optics Society
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• v.18 no.2
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• pp.187-191
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• 2013

Purpose: This study was done to understand the visual system of bat by analyzing the distribution of middle/long (ML) opsin cone photoreceptors in the retina of the greater horseshoe bat. Methods: Experiments have been performed by standard immunocytochemical techniques on retina of the greater horseshoe bat Rhinolophus ferrumequinum. Results: The estimated numbers of ML cones were $27,336{\pm}2,130$ cells and the mean density of them was $7,854{\pm}268cells/mm^2$ among the four retinas. S opsin was appeared a little immunoreactivity in the outer segments of outer nuclear layer of cones. Conclusions: From the well organized spatial distributions of ML opsin and the immunoreactivity of S opsin in the retinas, the greater horseshoe bats have the functions not only reacting in the photopic vision but being able to distinguish the colors.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.3
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• pp.413-418
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• 2014

Purpose: The objective of this study was analyzing the distribution of the excitatory neurotransmitter glutamate receptor to investigate the function in the retina of the greater horseshoe bat. Methods: After retinal tissues of adult greater horseshoe bat were cut into $40{\mu}m$ vertical sections, standard immuno-cytochemical techniques was applied for analysis. Immunofluorescence images were obtained using the Bio-Rad MRC 1024 laser scanning confocal microscope. Results: AMPA (GluR1-4), Kainate (GluR5-7, KA1-2) and NMDA (1, 2A, 2B) mainly distributed in the inner plexiform layer and outer plexiform layer. KA1 receptors have existed not only plexiform layer but also ganglion cell layer. Conclusions: The greater horseshoe bat has same neuron and neurotransmitter to mammalian retina. These findings suggest that bat has a functional retina for visual analysis.

• Journal of Korean Ophthalmic Optics Society
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• v.12 no.4
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• pp.133-139
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• 2007

Although the physiological roles of calretinin have not been established, it may simply work as a calcium buffer or may actively work in calcium-mediated signal transduction. Calretinin plays a little role in the transport and physiological buffering of calcium in the adult photoreceptor cells, bipolar cells and horizontal cells of the human retina. We identified the calretinin-immunoreactive neurons in the inner nuclear cell layer and ganglion cell layer and the distribution pattern of the labeled neurons in the retina of a bat, Rhinolophus ferrumequinum, in this study. We observed the existence of calretinin-immunoreactive AII amacrine cell in the inner nuclear layer and ganglion cells in the ganglion cell layer of bat retina through this study. This observation must be significant along with our previous studies as we need to study for more understanding about the unsolved issue of a bat vision and the unique behavioral aspects of bat flight maneuverability.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.323-330
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• 2006

The morphology and somatic chromosome numbers of Korean Parasenecio were investigated to estimate their taxonomic values. Some morphological characters, such as the length of whole plants, the numbers of cauline leaves, the shapes of leaf and petioles, the numbers of involucral bracts, floret numbers per head, presence of trichomes on the style, ratio between wide part and narrow part of floret and the size of achene were found to be a good characters for delimiting each taxa. Based upon these characters, six taxa, Parasenecio auriculala var. auriculata, P. auriculata var. kamtschatica, P. hastata subsp. orientalis, P. koraiensis, P. firmus, P. pseudotaimingasa, were recognized about their distribution in Korea, but 2 taxa, P. adenostyloides, P. auriculata var. matsumurana, were needed to be studied their taxonomic position and distribution in Korea. The somatic chromosome numbers of six taxa, P. adenostyloides, P. auriculata, P. auriculata var. kamtschatica, P. hastata subsp. orientalis, P. firmus, P. pseudotaimingasa were 2n=60 and those of P. firmus and P. pseudotaimingasa were reported first in this study.

• The Korean Journal of Zoology
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• v.31 no.4
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• pp.309-317
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• 1988

내륙산 관박쥐와 제주산 관박쥐간의 동-종 여부를 조사하기 위하여 경남과 제주에서 채집한 이들 관박쥐들을 핵형분석하였다. 핵형분석의 결과, 2종 모두 염색체수는 2n=58이었으며 FN도 62였다. 상염색체에서는 중형의 2쌍과 미세형 1쌍이 중부염색체(metacentrics)였고, 대형에서 소형까지는 25쌍이 단부염색체facrocentrics)이었다. 성염색체에서 X염색체는 대형의 차중부염색체(submetacentrics)였고, Y염색체는 소형의 단부염색체(acrocentrics)이었다. 그리고 이들 염색체들은 동원체 부근에 이질염색체을 가지는 특이한 1쌍의 단부염색체(acrocentrics)가 존재하고 있었다. in this study, We analysed the karyotypes of the inland bat(Rhinolox)thus femequinum hora) and the Cheju-Island bat(Rhinorophus ferrumequinum quelpartis(\ulcorner)) collected in Kyungnam and Cheju provinces to identify the homogeneous between them. The results are as follows. The diploid number of chromosomes of them are equally 58 and the fundamental number 62. In the autosomes, metacentrics consist of two pairs of the middle form and a pair of the micro-form. And acrocentrics have 25 pairs of large and small form. In sex-chromosomes, X-chromosome is a large submetacentrics and Y is a small acrocentrics. And, these chromosomes possess a pair of particular acrocentrics having heterochromatin around centromere in both the inland bat(Rhinolophus fenmequinum korai ) and Cheju-Island bat(Rhinolophus fewmequinum querporis (\ulcorner)).

• Applied Microscopy
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• v.41 no.2
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• pp.117-122
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• 2011

Sperm morphology of Pipistrellus savii velox was examined by transmission electron microscope. The sperm head of P. savii velox was bullet in shape. The sperm head was 3.1 ${\mu}m$ in length, whose posterior 3.0 ${\mu}m$ was occupied by a nucleus with 1.8 ${\mu}m$ in width. The segmented columns were about 14~15 in number. The total number of mitochondrial gyres was 57. Number of 1, 5, and 6 of the outer dense fibers were larger than the others. A fibrous sheath and longitudinal column of the principal piece were evidence, but the fibrous sheath was not seen at the end piece. In the present study, the length of the sperm head of P. savii velox were very shorter than those of other bats.

• The Korean Journal of Zoology
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• v.33 no.2
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• pp.127-132
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• 1990

The visual discrimination ability of Vespertilio superans was investigated with a male and a female. In a controlled experimental situation (two-choice apparatus), using a vertical bar and a circle of the same area of the bar, it was established that discrimination between different shapes of the same size was impossible. However, with patterns in which the surface areas substantially differed, the bats were able to discriminate between them to a statistically significant degree. Continuing with experiments in which the lightness of the 2 paritems was varied, the disrimination was possible with all but the slightest differences in lightness (a difference of lightness value 2).