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http://dx.doi.org/10.14479/jkoos.2015.20.3.391

Localization of the Major Retinal Neurotransmitters and Receptors and Müller Glia in the Retina of the Greater Horseshoe Bat (Rhinolophus ferrumequinum)  

Lee, Jun-Seok (Dept. of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University)
Kwon, Oh-Ju (Dept. of Optometry, Busan Institute of Science and Technology)
Jeon, Tae-Heon (Boston Trinity Academy)
Jeon, Chang-Jin (Dept. of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University)
Publication Information
Journal of Korean Ophthalmic Optics Society / v.20, no.3, 2015 , pp. 391-396 More about this Journal
Abstract
Purpose: The objective of this study was to investigate the visual system of the greater horseshoe bat (Rhinolophus ferrumequinum) by location analysis of some major neurotransmitters glutamate, ${\gamma}$-aminobutyric acid (GABA), acetylcholine, and their receptors, and $m{\ddot{u}}ller$ glial cells in retina. Methods: Standard immunocytochemical techniques were used after vibratome section of retinal tissues of adult greater horseshoe bat for this study. Immnoreactions in immunofluorescence images were analyzed using confocal microscope. Results: Anti-glutamate-immunoreactive neurons were mainly localized in the ganglion cell layer (GCL). The majority of anti-GABA-immunoreactive cells distributed in the inner nuclear layer (INL), and GABAA receptors were localized in the inner plexiform layer (IPL). Anti-choline acetyltransferase-immuoreactive cholinergic neurons were mainly located in the INL and GCL, and most of nicotinic acetylcholine receptors were localized in the IPL. The $m{\ddot{u}}ller$ cells in the retina of the greater horseshoe bat stretched theirs range from the GCL to outer nuclear layer (ONL). Conclusions: This study revealed that the retinas of the greater horseshoe bats contain the same major neurotransmitters and receptors, and glial cell in visually functional mammalian retinas. The present results may suggest that the greater horseshoe bats have the functional retinas for visual analysis through the organized retinal neural circuits.
Keywords
Acetylcholine; ${\gamma}$-aminobutyric acid; Glutamate; Immunocytochemistry; $M{\ddot{u}}ller$ cell; Retina; The greater horseshoe bat;
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Times Cited By KSCI : 5  (Citation Analysis)
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