• Journal of Korean society of Dental Hygiene
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• v.17 no.6
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• pp.1147-1157
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• 2017

Objectives: Dental implants are one treatment method for tooth loss. The purpose of this study is to investigate the status of experience and intention for dental implants and related factors among elderly. Methods: The participants were 244 elderly residing in rural areas with age over 65 in Samhoeup, Yeongamgun and Jeollanamdo, Korea. Chi-square tests, t-tests, and a multiple logistic regression analysis were performed to investigate the factors influencing the experience and intention of implants. Results: Many elderlies residing in rural areas rural elderly had experienced tooth loss (83.6%). The proportion of dental implant experience was 30.9% and intention to receive implants was 57.8%. The odds ratio (OR) for experience of implants was significantly higher for participants with a lower number of chronic diseases (0: OR=12.48, 95% CI=2.12-73.38, 1: OR=4.95, 95% CI=1.66-14.72), they have dental treatment experience during the past 1 year (OR=6.61, 95% CI=1.83-23.89), they have surrounding person who have experience of implant (OR=18.06, 95% CI=2.81-115.99), higher dental implant recognition (OR=3.97, 95% CI=1.92-8.23). The odds ratio (OR) for dental implant intention was significantly higher for participants with a lower age (65-69: OR=8.18, 95% CI=2.38-278.08, 70-79: OR=3.17 95% CI=1.04-9.68), lower number of chronic diseases (0: OR=4.15, 95% CI=1.00-17.29), they have drink alcohol (OR=5.03, Cl=1.31-19.34), they have surrounding person who has experience of implant (OR=3.22, 95% CI=1.30-8.02), they have not experience of tooth loss (OR=4.65, Cl=1.22-17.70), higher dental implant recognition (OR=2.69, 95% CI=1.55-4.67). Conclusions:In the future, it is necessary to utilize the results of this study to address loss of teeth and to support dental implant treatment selection through improved increased awareness of the advantages and disadvantages of dental implants.

• Research in Plant Disease
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• v.15 no.3
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• pp.193-197
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• 2009

Pseudocercospora leaf spot was major disease of grape cultivar 'Campbell Early' in Korea. Leaf spot first appeared in early June and rapidly dispersed to other leaves through rainy season. Disease progress of leaf spot by Pseudocercospora vitis in plastic green house, in the two provinces (Gimje and Gimcheon), were investigated in 2007. Differences of Infected leaves (%) between cultivation systems were observed in field and plastic green house, but there was no difference between provinces. Micro environmental factors, such as temperature and relative humidity, were correlated with infected leaves by PROC REG procedure of SAS (Statistical Analysis System). As a result, regression model best described ($R^2=0.95^{**}$) the infected leaves as a function of the interaction of cumulated temperatures; Y (Infected leaves)=-7.0101+0.0496$\times$20Hcum (Cumulated hour above 20 degree)+0.0208$\times$20cum (Cumulated temperature above 20 degree)-0.2781$\times$25Hcum (Cumulated hour above 25 degree). A statistics model was shown that cumulated hour and temperature above specific degree were critical factor for Pseudocercospora leaf spot on the grapevine leaves in plastic green house.

• Research in Plant Disease
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• v.15 no.3
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• pp.198-201
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• 2009

Bacterial wilts, caused by Ralstonia solanacearum, is a very destructive disease to tomato plants (Solanum lycopersicum) in Korea. Selection of resistant cultivar is the best way to prevent or reduce the occurrence of this disease. Thirty-nine tomato cultivars, twenty-one cherry tomato cultivars and thirteen rootstock tomato cultivars were inoculated with Ralstonia solanacearum, to evaluate tomato cultivars for resistance against bacterial wilts. Thirty-seven cultivars were highly susceptible to bacterial wilts and 61-100% of their whole tissue became wilted within 10 days after inoculation. Twenty-four cultivars showed moderate resistance and twelve were resistant to bacterial wilts. In an evaluation of 73 major commercial cultivars, 'Lilyance', 'TP-7', 'Choice', 'Dadaki', 'Akiko', 'Redstar', 'Match', 'B-blocking', 'Magnet', 'Support', 'Friend' and 'Special' were found to have a high level of resistance to bacterial wilts of tomatoes.

• Research in Plant Disease
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• v.15 no.3
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• pp.230-235
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• 2009

Postharvest skin sooty dapple and decay disease of pear fruit often originates at small stain symptoms that occurred during harvest and handling. Experiments were conducted to characterize the effect of timing of application of disease control materials, and to evaluate sequential postharvest applications of fungicides or fungicides and bio-control agents. Fungicides and bio-control agents were increasingly less effective when the period between harvest and application was prolonged. Thiabendazole (TBZ) applied to fruit without artificial wounding or inoculation effectively reduced skin sooty and decay disease when applied within 3 weeks or 6 weeks in 2 years of study. TBZ, Fludioxonil and pyrimethanil were effective in controlling skin sooty and decay disease at artificial wounds inoculated with Cladosporium tenuissimum up to 14 days after inoculation. Application of TBZ at harvest followed 3 weeks later by application of Fludioxonil was superior to application of TBZ at harvest alone. Two bacterial biocontrol agents reduced skin sooty and decay disease at pear wounds inoculated with C. tenuissimum up to 14 days after inoculation with C. tenuissimum, but were ineffective when applied at 28 days after inoculation. Of possible sequential arrangements of fungicide and bio-control treatments, application of the most effective material promptly after harvest generally resulted in the highest level of disease control.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.58-65
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• 2016

Powdery mildew is an important disease of the melon (Cucumis melo L.). Seven isolates of powdery mildew fungi were collected from five locations in Korea; Anseong (DH487), Icheon (BN103, BN625, BN968), Yeongam (YA141), Changnyeong (CN582), and Suncheon (SN102). All 7 fungi had a similar trend of conidial chain and conidiophore development as Podosphaera xanthii with fibrosin bodies in mature conidia. Among them, 2 isolates of powdery mildew fungi; CN582 and SN102 showed similar responses to resistance against powdery mildew as the previously reported race 1 and race N2. The isolates YA141 and BN103 showed similar responses as like as race A. However, three isolates of powdery mildew fungi (BN625, BN968, and DH487) showed different responses compared to the previously reported races (1, N1, N2, A, S, and 5). Therefore, these three isolates could be designated as new races in melon. Nine out of 15 commercial melon cultivars in Korea showed resistance to race 1 (CN582). However, the new race BN968 invaded all 15 cultivars. Results of the two molecular markers were consistent in response to disease development by race 1 of Podosphaera xanthii in case of the above mentioned cultivars. This study confirmed the presence of new melon powdery mildew fungi in Korea which are similarly notorious as like as the previously reported race 1. Therefore, breeders can use these two molecular markers for breeding melon in Korea that is resistant to race 1 and as well as to some other races.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.174-185
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• 2010

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. To elucidate the functions of a large population of Chinese cabbage (Brassica rapa) genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the full-length cDNA Over-eXpresser (FOX) gene hunting system. With oligo dT column it purify the each mRNA from the flower organs, leaf and stem tissue. And about 120,000 cDNAs from the library were transformed into $\lambda$-pFLCIII-F vector. Of which 115,000 cDNAs from the library were transformed into T-DNA binary vector, pBigs for transformation study. We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. Full-length Chinese cabbage cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Selfed seeds were harvested from transgenic Arabidopsis. We had selected 2,500 transgenic plants by hygromycin antibiotic tolerant test, and obtained a number of transgenic mutants. Each transgenic Arabidopsis was investigated in morphological changes, fertility and leaf colour. As a result, 285 possible morphological mutants were identified. Introduced cDNA was isolated by PCR amplification of the genomic DNA from the transgenic mutants. Sequencing result and BLAST analysis showed that most of the introduced cDNA were complete cDNAs and functional genes. Also, we examined the effect of Bromelain on enhancing resistance to soft rot in transgenic Chinese cabbage 'Osome'. The bromelain gene identified from FOX hunting system was transformed into Chinese cabbage using Agrobacterium methods. Transformants were screened by PCR, then RT-PCR and real time PCR were performed to analyze gene expression of cysteine protease in the T1 and T2 generations. The anti-bacterial activity of bromelain was tested in Chinese cabbages infected with soft rot bacteria. The results showed that the over-expressed bromelain gene from pineapple conferred enhanced resistance to soft rot in Chinese cabbage.

• Research in Plant Disease
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• v.12 no.1
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• pp.10-14
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• 2006

This study was carried out to know damages of the grapevine trees by the disease to the leaf spot from 2000 to 2003. The isolates collected from different varieties and locations were identified as Pseudocercospora vitis ($(L\acute{e}v)$.) Speg. based on the morphological and cultural characteristics. According to the in vitro test, the range of temperatures for the mycerial growth and the conidical germination of the fungus were from $10^{\circ}C\;to\;35^{\circ}C$ and optimum temperature was $25^{\circ}C$. There were remarkably different features between a low infection trees group and high infection trees group in terms of number of leaves per fruit branch, length and diameter of internode and leaf area.

• Research in Plant Disease
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• v.12 no.1
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• pp.40-45
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• 2006

The plant defence activator, Acibenzolar-S-methyl [benzo (1,2,3) thiadiazole-7-carbothioic acid-S-methyl ester, ASM] was assayed on tomato seedlings for its ability to induce resistance against Botrytis cinerea, the causal agent of gray mold in tomato. Pre-treatment of plants with ASM reduced the severity of the disease as well as the growth of the mycelium in plants. In ASM treated plants, reduction in disease severity (up to 55%) was correlated with suppression of mycelia growth (up to 46.5%) during the time course of infection. In plants treated with ASM, activities of peroxidase were determined as markers of resistance. Applications of ASM induced Progressive and significant increase of the enzyme in locally treated tissues. Such responses were expressed earlier and with a much higher magnitude when ASM-treated seedlings were challenged with the pathogen, thus providing support to the concept that a signal produced by the pathogen is essential for triggering enhanced synthesis and accumulation of the enzymes. No such activities were observed in water-treated control plants. Therefore, the slower symptom development and reduction in mycelium growth in ASM treated plants might be due to the increase in activity of oxidative and antioxidative protection systems in plants.

• Research in Plant Disease
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• v.15 no.3
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• pp.259-261
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• 2009

Sclerotinia shoot rot of Grapevine(Vitis labruscana) occurred at Gapyeong and Yeongwol area from 2003 to 2005. Infected plants showed shoot blight at the one-year-old fruit bearing branches. The first visible symptom noticed was wilting and blighting of the branches. The obvious and typical initial symptom is the presence of a cottony, white, dense mat of mycelial growth on the surface of the diseased lesions. The base or stem of the infected young shoots develop a pale brown rotted area, which girdled and killed shoots. In advanced stages of the disease, stems and branches became bleached and eventually died. The isolates collected from diseased grapevine stem were identified as Sclerotinia sclerotiorum based on the morphological and cultural characteristics. The pathogenicity test revealed that Vitis vinifera(cultivar: Kyoho) was stronger than V. labruscana(cultivar: Campbell early) to the pathogen. This is the first report on sclerotinia shoot rot of grapevine in Korea.

• Journal of the Korea Safety Management & Science
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• v.22 no.3
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• pp.1-8
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• 2020

The desulfurizers facility is cylindrical shape. To operate properly it needs nozzles cleaned, get rid of lime adhesion and sludge, repair the wear and corrosion of facility regularly. For this purpose, workers shall access the ceiling or vertical wall at high place. Ordinary scaffoldings such as steel pipe scaffolding or system scaffolding have been using so that workers can access them. With these ordinary scaffoldings, openings around cylindrical wall are inevitable which make workers can expose always to the risk of falling. The purpose of this study is to develop customized scaffolding to minimize the openings to prevent workers form falling during maintenance it. It consists of a hexagonal central tower and six trapezoidal outer towers. And the bracing among the towers have connected each other for self-standing and for maintaining the structure of towers. Span decks, the circumference footstools, steps, etc. are laid on each floor. The safety is reviewed by structural analysis and performance test. With this study, openings each floor of this scaffold are removed. The gap between the cylindrical wall and the edge of the work stage is approximately 100 mm. Therefore, we expect that workers can work safely and efficiently.