• Korean Journal of Food Science and Technology
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• v.47 no.3
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• pp.321-327
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• 2015

The optimal condition for preparation of powdered calcium acetate (LCCA) which has high solubility, from calcined powder (LCCP) of the littleneck clam shell by response surface methodology (RSM) was examined. Increased molar ratio of LCCP led to reduced solubility, yield, color values, and overall quality. The critical values of multiple response optimization of independent variables were 2.57 M of acetic acid and 1.57 M of LCCP. The actual values (pH 7.0, 96.1% for solubility, and 220.9% for yield) under the optimized condition were similar to the predicted values. LCCA showed strong buffering capacity between pH 4.89 and 4.92 on addition of ~2 mL of 1 N HCl. The calcium content and solubility of LCCA were 21.9-23.0 g/100 g and 96.1-100.1%, respectively. The FT-IR and XRD patterns of LCCA were identified as calcium acetate monohydrate, and FESEM images revealed an irregular and rod-like microstructure.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.217-220
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• 1981

A trematode, Cercaria tapidis which is parasitic to short necked clam, Tapes philippinarum was studied in terms of its morphology and incidence of infection rate. The host bivalve was collected from Solri near Gunsan from September 1980 to August 1981. Sporocysts were observed mainly in tissues of gonad of the short necked clams. Minimum infection rate ($0.85\%$) was found in May, while maximum infection rate ($23.27\%$) in December. The sporocyst is 1.1 mm long and 0.27 mm wide. Ellipsoidal body of cercaria is $283{\mu}m$ long and $120{\mu}m$ wide. Oral sucker is much larger than ventral sucker. Moderately small pharynx, a long esophagus, and a long intestine reaching to the posterior end of the body are distinctive. Globular excretory bladder is located at the posterior part of the body and bears numerous granules of various size. The flame-cell arrangement is represented by a formula 2[(3+3+3)+(3+3)]=30. Tail is five times body length.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.207-216
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• 2013

Changes in density of manila clam, Ruditapes philippinarum to environment, catch and recruitment were studied in a few stations (Seonjae, Seongam, Hwangdo and Padori) in the West coast area of Korea from January, 2007 to December, 2009. Water temperature, salinity, dissolved oxygen and pH in the study stations were 0.8 to $31.2^{\circ}C$, 22.1 to 33.7 psu, 5.0 to 12.0 mg/L and 7.39 to 8.99, respectively. The concentrations of dissolved inorganic nitrogen, phosphate and silicate were 0.016 to 1.281 mg/L, 0.004 to 0.093 mg/L and 0.016 to 1.617 mg/L, respectively. Chlorophyll-a ranged from 0.2 to 12.1 ${\mu}g/L$, respectively. Substrata were mainly composed of muddy sand and very poorly sorted in Padori, muddy sand and well sorted in Seonjae and Hwangdo, gravelly muddy sand and poorly sorted in Seongam. Density was high in Seonjae and Seongam, but low in Hwangdo and Padori. In clam culture station, in which spat was naturally produced without sowing seedlings, the living density was decreased by increasing of death and a catch of shellfish, and recruitment was changed. Also, Density affected condition factor and shape of clam. Condition factor was the highest in Hwangdo, in which temperature in the winter and chlorophyll-a were high, and was the lowest in Padori. In the shape of clam, the shape in Seongam was a elongated form, but in Padori was a stunted form.

• Journal of Aquaculture
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• v.6 no.3
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• pp.133-146
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• 1993

Monthly variation of fatness index of the shortnecked clam was investigated at Moonhangri in Namhaedo of the southern coast of Korea from April 1991 to March 1992. The standing crops of umbo stage larvae and spats of this bivalve were also investigated. The results are as follows: The ranges of a monthly average water temperature and salinity were $6.9\~23.5^{\circ}C$ and $29.6\~33.1%o$ during the investigation period. Fatness index of the shortnecked clam decreased suddenly from June to September, which seemed to be spawning season. The umbo stage larvae of the clam were observed from May to November. The standing crops of the larvae were $883\;inds./m^3$ in August and$68\;inds./m^3$ in November. The observed number per ~ of the spats varied with the month and the different exposure time zones. The highest density $(1,508\;inds./m^3)$ was observed 1 hour exposure zone in August, and the lowest one $(28 inds./m^3)$ at 1 hour exposure station in April. Even the observed number of the spats below 4 mm in shell length were high in 1 and 3 hour exposure zones, the spats above 4 mm in shell length were observed mainly at 3 hour exposure zone.

• Journal of Food Hygiene and Safety
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• v.3 no.1
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• pp.7-18
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• 1988

In 1987, the level of heavy metals were determined ina total of 200 samples of 9 species of shellfish of Korea. The samples were collected at the fish. markets by 10 Public Institute of Health. The samples were whelk (Buccinum striatiBBimum), oyster (Crassostrea gigas), ark shell(Tegillarca granesa), shartnecked clam (Venerupis semidecussta), hard clam (Meretrix lusoria), top shell (Turbo cornutus), abalone (Haliotis gigantea), ark shell (Scapharea broughtonii), sea-mussel (Mytilus conuscus gould), respectively. The levels of total mercury, lead, cadmium, arsenic, copper, zinc and manganese were determined. The total mercury levels were determined by mercury analyzer using the combustion gold amalgamation method. The arsenic level were determined by spectrophotometry using colorimetric sil ver diethyldithiocarbamate method after dry ash dige8tion of the samples with magnesium oxide and magnesium nitrate. The levels of other metals were determined by inductively coupled pluma spectrophotometry after wet digestion of the samples with nitric acid and su1furic acid. The results were summerized as follows; 1. The overallranges and mean(ppm) were; Hg, ND-O.221 (0.036); Pb, 0.05-1.51 (0.37); Cd, 0.02-1.86 (0.61); As, 0.5-3.97 (1.22); Cu, 0.14-54.16 (4.93); Zn, 7.40-207.17 (30.09); Mn, 0.13-s.72 (3.40). 2. The levels of all 6 metals were found to be below the maximum permissible Iimits set by the Japan lor mercury, the Netherland for lead the Hong Kong for cadmium. The Finland for Arsenic no statutory Iimits for Zn and Mn in shellfish in any countries. 3. The results show that all the 9 species of shellfish studied, none have accumulated levels dangerous enough to pose a health problem.roblem.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.5
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• pp.417-423
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• 1985

In succession to the previous paper, the present study was directed to investigate the nonvolatile organic acids composition in raw and belied-dried products of oyster, sea-mussel, baby clam and hen clam by gas liquid chromatography. The results obtained are summarized as follows : In four kinds of the samples examined, eight kinds of organic acids were identified and determined in oyster, sea-mussel and baby clam, and nine kinds in hen clam. The major organic acids in oyster were pyroglutamic, succinic and malic acid which was $94.2\%$ of total quantity of organic acid, and those in sea-mussel, baby clam and hen clam were succinic and malic acid which were $90.8\%,\;89.7\%\;and\;86.4\%$ of total acids, respectively. The most abundant organic acid in sea-mussel, baby clam and hen clam was succinic acid that was $80.6\%,\;84.9\%\;and\;73.2\%$ in total acids, repectively. And that of oyster was pyroglutamic acid which marked $38.8\%$ in total acids, and the next one was succinc acid marked $34.4\%$. In the total quantity of organic acid, the highest was 913.0 mg/100g in oyster which showed 4.5 times as much as in hen clam, followed by 478.4 mg/100g in sea-mussel, 246.3mg/100g in baby clam, and the least was 201.2 mg/100g in hen clam. The decreasing rate of total quently of organic acids by boiled-dried procersing was the highest in oyster, $54.7\%$, followed by $46.5\%$ in sea-mussel, $37.1\%$ in hen clam and $29.4\%$ in baby clam. The decreasing rate of each organic acid shelved much difference according to the samples examined, in general, great in malic, fumaric and proglutamic acid ana less in succinic, lactic and oxalic acid.

• Journal of fish pathology
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• v.18 no.1
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• pp.39-48
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• 2005

Pathogenicity of Vibrio tapetis, the causative bacterium of 'brown ring disease (BRD)' was evaluated in Manila clams (Ruditapes philippinarumi by artificially 0.1 $m\ell$ infection of $1.0\times10^5$cells and $1.0\times10^8$ cells at 20 $^{\circ}C$. A PCR assay based on 16S rRNA to detect the bacteria in clam tissues was established. Accumulative mortality of clams infected with $1.0\times10^7$cells and $1.0\times10^4$ cells per an individual of the bacteria was 67.5% and 7.5%, respectively. However, the deposit of brown pigment in the inner shells by accumulation of chonchiolin was not found. The bacteria were not be able to re-isolate from the infected clams by the conventional agar plate method but were easily detected by PCR assay established in this experiment. In clams artificially infected with 10 species of Vibrio, a 414bp for V. tapetis was detected in PCR assay. The specific band in the clams infected with $1.0\times10^4$cells per an individual of V. tapetis was detected only in gills one day after the infection but never be found in any tissues including gills three days after the infection. In the case of clams infected with $1.0\times10^8$cells per an individual of V. tapetis the specific band was detected in gills and intestine one day after the infection, in all tissues three days after the infection, and then in gills and adductor muscle nine days after the infection. The PCR assay was applied to detect V. tapetis in manila clam, surf clam (Mactra veneriformis), oyster (Crassostrea gigas) and Thomas' rapa whelk (Rapana venosa) taken from Taean and Gochang from April to July 2004. The infection rates were detected to 23.1% and 9.4% in the oyster and surf clam, while manila clam and Thomas' rapa whelk were not found.

• The Korean Journal of Malacology
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• v.27 no.2
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• pp.107-114
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• 2011

The manila clam (Ruditapes philippinarum) is mainly distributed in the coastal area which consist of mud, sand and gravel, but they rarely live on the upper and down reaches of river. For a long time the manila clam has been inhabited in Taehwa river which has been exploited as a traditional earning resources and has become as a major object by neighborhood fishermen. This study was undertaken to evaluate stock assessment and to build management implications with the ecological parameters in Taehwa river from June 2009 to June 2010. The maximum age of manila clam was determined to 6 years old from observing ring radius of shell, the length and weight relationship was TW = $0.0002SL^{3.063}$ ($R^2$ = 0.925). K and $L_{\infty}$ were respectively estimated 46.64 mm and 0.341/year by von Bertalanffy growth. The instantaneous total mortality was estimated to be 1.171/year and the age at first capture was 1.37 years by the Pauly's method using shell length composition. The current total biomass of manila clam was calculated 1,483 mt over study area $1.46\;km^2$ by swept area method. ABC (Acceptable Biological Catch) estimates of manila clam showed 512 mt with using $F_{0.1}$. It's desirable to determine the optimum harvesting time as after main spawning season, as well as it's required to manage fisheries resources considering capture age and biomass through adjusting a first age at capture.

• Proceedings of the Malacological Society of Korea Conference
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• 2001.11b
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• pp.138-139
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• 2001

• Proceedings of the Malacological Society of Korea Conference
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• 2001.11b
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• pp.141-143
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• 2001