• Korean journal of applied entomology
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• v.22 no.3 s.56
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• pp.198-202
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• 1983

A mosaic disease of gladiolus has been commonly observed with an infection rate of $43.3\%$ in the field. Bean Yellow Mosaic Virus(BYMV) produced veinal spreading lesions on Cheonopodium amaranticolor, veinal necrosis and severe leaf distortion on Phaseolus vulgaris 'Scotia' and mosaic on Vi cia faba. Cucumber Mosaic Virus(CMV) produced local lesions on C. amaranticolor, mosaic symptoms on Nicotiana glutinosa and Cucumis sativus. BYMV and CMV were transmitted by the green peach aphid. Purified BYMV and CMV had a typical maximum absorption at 260nm. In agar gel diffusion test, BYMV and CMV gave positive reaction with their homologous antiserum. The size of BYMV was 750nm in length, and CMV was 30nm in diameter.

• Korean journal of applied entomology
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• v.14 no.4 s.25
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• pp.193-198
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• 1975

This experiment was performed to clarify the concentration of rice stripe virus in the rice Plant leaves by serological test, and was attempted to inspect the virus carrier among small brown planthopper by antibody-sensitized hemagglutination test. The antiserum was prepared by injecting intervenously into the external marginal vein of the ear of a rabbit. The precipitin titer of it was 1 : 16. The rough virus fluid prepared from diseased leaves was centrifuged at 10.000 rpm, and then the supernatant solution was treated at $55^{\circ}C$ for 5 minutes and the solution clarified by removing the agglutinate was used as the antigen solution. Antibody-sensitized erythrocyte solution was prepared from sheep erythrocytes sensitized by rice stripe virus with tannic acid, and its agglutination titer was 1 : 512. The virus concentrations in flag leaves or first leaves just below them showing different symptoms was high with progressing the severity of symptoms. And the concentrations of the virus in leaves of varieties of the rice plant showing same degree symptom were lower in suscetible varieties, Sadominori, Palgoeng, Mangyong and Nihonbare, than in the resistant one, Tongil, but in Yooshin which was known as the resistant, lower rather than in Tongil. The reacton of antibody-sensitized hemagglutination test to inspect the virus carrier, was so highly sensitive that this reaction was recognized as a method which is able to Identify the carrier accurately in short time.

• Journal of Life Science
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• v.19 no.7
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• pp.928-933
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• 2009

In an effort to discover an antiviral substance against noroviruse (NV), which causes gastroenteritis illness world-wide, several plants including spices and herbs were evaluated for their antiviral activities against feline calicivirus (FCV) as a surrogate for NV. Among them, methanolic extract of green tea (Camellia sinensis L.) exhibited significant antiviral activity against FCV. After treatment with green tea extract (3.13 mg/ml) for 1 hr, FCV was completely inactivated. The antiviral activity of green tea extract against FCV was also determined to be dose and time- dependent. The results obtained in this study suggested that green tea will be effective in the prevention of food-borne diseases caused by NV.

• Journal of Sericultural and Entomological Science
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• v.34 no.2
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• pp.26-31
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• 1992

Flacherie virus (FV) and Densonucleosis virus (DNV) of the silkworm, Bombyx mori, which give the most severest damage to the silk production in korea, were fed on the mulberry wild silkworm, Bombyx mori mandarina, the mulberry pyralid, Gryphodes phyloalis, and the American fall webworm, Hypantria cunea, to investigate cross infectivity by serological and histopathological at observation. By the Ouchterlony's double difusion test the mulberry wild silkworm was infected with both FV and DNV type 1 (DNV-1) and the mulberry pyralid with DNV-1, so those were confirmed the cross infection. But the American fall webworm was not recognized the cross infection by the same method. The infection and multiplication of the FV in the mulberry wild silkworm was observed in the cytoplasm of the goblet cell with the appearance of the virus-specific vesicle. In DNV-1 infection to the mulberry wild silkworm and the mulberry pyralid, the nuclei of columnar cell in the midgut of both insects was hypertrophied and the nuclei of midgut cell of the mulberry pyralid positively stained with the feulgen stain. Multiplication of DNV-1 in the midgut cell of the mulberry wild silkworm was replicated in two different patterens as linear arrays and large masses, while that of DNV-1 in the muberry pyralid was multiplied as virus masses in several portion of the nuclei of the midgut cell.

• Biomedical Science Letters
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• v.6 no.2
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• pp.141-149
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• 2000

The etiologic agents of haemorrhagic fever with renal syndrome (HFRS) in Korea are Hantaan and Seoul virus in the genus Hantavirus, family Bunyaviridae. Antibody titers of sera from HFRS patients against Hantaan virus were measured by immunofluorescent antibody technique (IFAT), enzyme-linked immunosorbent assay (ELISA), high density composite particle agglutination (HDPA) and plaque reduction neutralization test (PRNI). PRNT and nested reverse transcriptase polymerase chain reaction (nested RT-PCR) was used for serotypic differentiation of Hantaviruses against Hantaan and Seoul virus. Eight doubtful HFRS patients showed higher fluorescent, IgG ELISA, agglutination and neutralizing antibody titer by IFAT, ELISA IgG, HDPA and PRNT, respectively Five out of them showed high IgM antibody titer by IgM capture ELISA against Hantaan virus, remarkably. Fifteen HFRS patients showed higher fluorescent antibody titer by IFAT. In PRNT, 12 out of them showed high neutralizing antibody titer against HTNV, 2 against SEOV and 1 against both viruses. In nested RT-PCR using serotype specific-primer, 3 out of them showed positive against HTNV and 1 against SEOV.

• Research in Plant Disease
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• v.23 no.1
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• pp.60-64
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• 2017

Leaves of twenties radish (Raphanus sativus L.) inbred lines were mechanically inoculated with Turnip mosaic virus (TuMV) strain HY to evaluate TuMV resistance of the radish inbred lines. The inoculated radish plants were incubated at $22^{\circ}C{\pm}3^{\circ}C$ and resistance assessment was examined using symptom development for 4 weeks. Based on the reactions of differential radish inbred lines, 16 radish lines were produced mild mosaic, mottling, mosaic and severe mosaic symptoms by TuMV infection. These results were confirmed by RT-PCR analysis of TuMV coat protein gene, suggesting that TuMV is responsible for the disease symptoms. Four resistant radish lines did not induce systemic mosaic symptoms on upper leaves and chlorosis in stem tissues for 4 weeks, showing they were symptomless by 8 weeks. Further examination of TuMV infection in the 4 radish lines showed no TuMV infection in all systemic leaves. These results suggest that the 4 radish lines are highly resistant to TuMV.

• Journal of fish pathology
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• v.13 no.1
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• pp.53-59
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• 2000

Presence of marine birnavirus (MABV) was examined against egg and ovarian fluid, and seminal fluid from the brood stocks of flounder, Paralichthys olivaceus collected from 9 different stations around Korean peninsula. The detection rate of MABV in brood stocks flounder was observed to 34% by PCR. The mean virus titer of the PCR positive fish was $10^{2.30}$ to $10^{4.30}$ $TCID_{50}$/g(ml). By a neutralization test, all of the isolated virus were ascertained to be closely related to marine birnavirus (MABV).

• Pediatric Infection and Vaccine
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• v.14 no.2
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• pp.171-178
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• 2007

Purpose : We investigated clinical characteristics of real-time PCR proven EBV viremia patients who were not serologically diagnosed but clinically suspected, and compared it to serologically proven EBV infected patients. Methods : The study population consisted of 45 patients, who were suspected acute EBV infection at Wonju Christian Hospital Department of Pediatrics, Yonsei University Wonju College of Medicine from Jan. 2004 to Dec. 2006. real-time PCR of cell free serum was performed to prove EBV viremia. Then we chose $102.5copies/{\mu}g$ DNA as a suitable cutoff level for EBV associated diseases. Results : There are 4 patients diagnosed as EBV infection by serologically and 15 patients diagnosed as EBV viremia by real-time PCR quantitative measurement. The most common presenting symptoms and signs of EBV viremia was fever in 11 cases (73%). Atypical lymphocytosis was found in 4 cases (27%). Increased AST, ALT levels were observed in 13 cases (87%), 12 cases (80%), respectively. We could diagnose 5 cases of EBV viremia younger than one year of age. They revealed clinical symptoms which could be found in EBV infection. The serologically diagnosed patients had hepatomegaly and splenomegaly in 3 cases (75%). All serologically confirmed patients have leukocytosis above $20,000/mm^3$, among them 2 cases (50%) had higher percentage (>15%) of atypical lymphocytes. The AST/ALT level above 50 IU/L were demonstrated in all cases. Conclusion : Serologically unproven real-time PCR EBV viremia patients revealed similar clinical findings with that of serologically proven EBV infected patients. So, it is meaningful to perform EBV real-time PCR for the diagnosis of EBV infection especially for the cases younger than 1 year of age.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.578-581
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• 2009

A 25-day-old, Korean indigenous calf was presented with a 10 days history of respiratory disorders and bloody diarrhea, and died. This calf was extremely unthrifty compared to others and had evidence of chronic diarrhea based on matting of feces in the hair of the tail and perineum. Ecchymotic hemorrhages were observed on multiple organs at necropsy. Bovine viral diarrhea virus (BVDV) infection was identified by RT-PCR. The phylogenetic analysis showed that this case belonged to BVDV-2a subgroup and was related to highly pathogenic USA isolate 890 (U18059). This case provided evidence for circulation of BVDV-2 in Republic of Korea. The occurrence of BVDV-2 was also reconfirmed.

• Korean Journal of Veterinary Research
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• v.30 no.4
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• pp.435-440
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• 1990

Ten pigs infected with Aujeszky's disease virus (ADY) or hog cholera virus(HCV) were tested for the detection of virus antigens in frozens or paraffin-embedded sections by avidin-biotin-peroxidase complex(ABC) method. Tonsils, spleens, cerebra and buffy coats were examined for the immunohistochemical test. Where ADV antigen was detected by ABC, a dark brown deposit occurred in both the nucleus and the cytoplasm of lymphocytes and macrophages, however, HCV antigen was demonstrated in the cytoplasm of the infected cells. ADV-positive cells were most frequently detected in tonsils and cerebra, whereas, HCV -positive cells were frequently observed in spleens. And buffy coat were also good for both virus detection. The results suggested that ABC method is considered as an excellent and reliable tool for confirmative diagnosis of these viral diseases.