• Title/Summary/Keyword: 바이러스살충제

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Microbial Control of the Tobacco Cutworm, Spodopera litura (Fab.), Using S. litura Nuclea Polyhedrosis Virus. II. Formulation of S. litura Nuclear Polyhdrosis Virus as Viral Insecticides (곤충 핵다각체병바이러스를 이용한 담배거세미나방의 생물적 방제. II. 담배거세미나방 핵다각체병바이러스의 살충제 제제화)

  • 임대준;진병래;최귀문;강석권
    • Korean journal of applied entomology
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    • v.29 no.4
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    • pp.244-251
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    • 1990
  • Three viral insecticides were differently formulated with a nuclear polyhedrosis virus isolated from Spdodoptera litura by addition of feeding attractant, anti-precipitate of polyhedra, spreading agent, and UV-protectants. Sucrose was effective for attraction of larval feeding to increase the mortality and for protection of polyhedra from inactivation by sunlight when added 1% to 5% of sucrose solution to the formulations. Contents of additives to the formulations were 0.5% in polyvinyl alcohol to prohibit the precipitation of polyhedra and 0.1% in Triton X-100 to spread and wet the formulations to the plant. Inactivation of the virus under sunlight was decreased when added 800g of white carbon to 100 L of water in the white carbon formulation and 30% of molasses to the molasses's. In the formulation of white carbon and molasses mixtures, activation of the virus was increased when mixtured 500g of the former with 10% of the latter. Three formulations were persisted their pathogenicity more than 95% of mortality at 3 days p.i. Encapsulation of the polyhedral surface was more distinctively coated with the carbon and showed more effective in the residual effects of the white carbon than others, but the molasses more attractive for larval feeding.

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Microbial Control of the Tobacco Cutworm, Spodoptera litura (Fab.), Using S. litura Nuclear Polyhedrosis Virus. III. Field Evaluation of the Viral Insecticides (곤충 핵다각체병바이러스를 이용한 담배거세미나방의 생물적 방제. III. 담배거세미나방 핵다각체병바이러스 살충제 살포효과)

  • 임대준;진병래;최귀문;강석권
    • Korean journal of applied entomology
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    • v.29 no.4
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    • pp.252-256
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    • 1990
  • Viral insecticides were formulated with Spodoptera litura nuclear polyhedrosis virus and different U.V. protectants based on white carbon, molasses, and white carbon and molasses mixture to use as microbial control agents. Effect of rainfall on the attachment of formulated viruses to leaves was no different between the treated and the non-treated experiment. Persistence of the formulations was lated 5 days on the surface-sprayed leaves and 12 days on the under-sprayed leaves which was showing 60% mortality. Total mortality of the viral insecticides was more than 97% with no differences among them. Field evaluation of three viral insecticides in soybean field was very successful then carried out in Chinju, a southern part of Korea. Mortality by the formulation in the field during 14 days was more than 93%, but the formulations contained molasses showed phytotoxicity on soybean leaves. Spray effect of the viral insecticides was begun to appear from 7 days later than that of chemical insecticide.

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Evaluation of Pesticide Treatment for Control of Rice stripe virus after Mass Migration of Small Brown Planthoppers (애멸구 대량 비래후 살충제 처리와 벼줄무늬잎마름바이러스(Rice stripe virus) 발생 관계 조사)

  • Jeong, Tae-Woo;Kim, Byung-Ryun;Han, Gwang-Seop;Kang, Dong-Woo;Jeong, Iim-Young;Lim, Hyoun-Sub;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.18 no.3
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    • pp.245-249
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    • 2012
  • The small brown planthopper (Laodelphax striatellus) is one of the most important rice pests in Republic of Korea because it damages rice plants not only by sap-sucking but also by transmitting Rice stripe virus (RSV). Outbreaks of RSV are closely related to outbreaks of the small brown planthopper (SBPH). Therefore, it is very important to control SBPH for the management of RSV. Mass-migrating SBPH collected by aerial net traps in June 2011 at Taeanup, Geunheungmyon and Gonammyon in Taeangun were examined for virus carrier status and effects of the pesticide, 'Myungtaja', on the control of RSV. Among 1,217 SBPH trapped, about 7.7% were detected as RSV positive and 4.4% were positive for Rice black streak dwarf virus (RBSDV) by RT-PCR. After the mass migration, pesticide 'Myungtaja' was sprayed once or twice on rice fields and compared to untreated fields. The incidence of RSV was not affected by the frequency of spraying 'Myungtaja' but was influenced by the time of pesticide treatment. Myungtaja' treatment within 5-7 days after mass migration resulted in the most efficient RSV control, resulting in RSV incidence decreased by 87.6% compared to the control. Therefore, we conclude that pesticide spraying for RSV control was most effective when it was done within 5-7 days after mass migration.

Structure of Spodoptera exigua Nucleopolyhedrovirus p10 Gene (파밤나방 핵다각체병 바이러스의 p10 유전자 구조)

  • 최재영;우수동;홍혜경;이해광;제연호;강석권
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.145-149
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    • 1999
  • To develop the baculovirus expression vector system (BEVS) adopting p10 gene promoter of Spodoptera exigua nucleopolyhedrovirus (SeNPV), we characterized the p10 gene of SeNPV. The nucleotide sequence of 545 bases including the coding region of p10 gene was determined. Compared with the previously reported SeNPV p10 gene (Zuidema et al., 1993), 4 bases were different in the 5' and 3' flanking region but no difference was found in the coding region. The p10 gene was located within Xho I 1.5 Kb, Sph 1 2.4 Kb and Cla I 4.0 Kb fragments by Southern hybridization analysis. Also, the Sph I 2.4 Kb and the Cla I 4.0 Kb fragments were cloned and their restriction enzyme maps were determined.

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Virus Purification by Membrane Chromatography: A Review (멤브레인 크로마토그래피에 의한 바이러스 정제 : 리뷰)

  • Gayatri Bhamidipatia;Rajkumar Patel
    • Membrane Journal
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    • v.34 no.2
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    • pp.124-131
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    • 2024
  • Viruses have various applications in the biopharmaceutical industry. They are used in pesticide production, production of vaccines, gene transfers, cancer therapeutics, and more. The downstream processing of viruses is an essential step for their biological and pharmaceutical applications. Among the various processes, the purification of viruses is critical. Membrane chromatography plays a vital role in this process. While ion exchange membrane chromatography is a primarily used method, it has various limitations regarding size exclusion and insufficient purification. Also, it cannot be applied to the rapidly changing strains of viruses such as influenza. This review examines various improved methods of membrane chromatography or alternatives. It focuses on purification, viral recovery rates, and scalability of the methods.

Electron Microscopy Studies on the Formation of Polyhedra Occlusion Bodies of Autographa californica Nuclear Polyhedrosis Virus (미생물 살충제인 Autographa californica Nuclear Polyhydrosis Virus의 Polyhydra 형성 과정의 전자현미경적 연구)

  • Lee Hyung-Hoan
    • Applied Microscopy
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    • v.11 no.1
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    • pp.51-57
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    • 1981
  • The process of the formation of polyhedra occlusion bodies and occlusion of viral nucleocapsids of Autographa californica Nuclear Polyhedrosis Virus in Spodoptera frugiperda cell were photomicrographed and described. Progeny viral nucleocapsids were observed in the nuclei of the host cells, bundled and then enveloped. The nucleoapsids were mainly accumulated near the membrane-like profiles. The nuclear membrane were hypertophied up to the cytoplasmic membrane. Prepolyhedral bodies were observed and they were growing with the accumulations of thread-like materials(polypeptides) produced by viral genes. The bundled and enveloped nucleocapsids were occluded into the growing polyhedra.

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Flying Aphid Population at the Horticultural Experiment Station, Suweon (원예시험장 주변의 진딧물)

  • Paik Woon Hah;Song Ki Won;Choi Seong Sik
    • Korean journal of applied entomology
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    • v.13 no.1 s.18
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    • pp.25-31
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    • 1974
  • This survey was aimed to accumulate basic data of aphid population at the Horticultural Experiment Station at Suweon. The yellow pan traps were setted at five locations (Fig.1.), and ran from May 1 to October 31. 1970. About one hundred and twenty species of aphids were trapped, including 24 species of plant vims vectors. Of these, dominant species were as follows: (Asterisk shows virus vector) Aphid species No. of catches * Aphis spiraecola PATCH 2,635, * Aphis craccivora KOCH 2,377, * Myzus persicae SULXER 2,111, Capitophorus hippophaes javanicus H.R. LAMBERS 2,051, Anoecia fulviabdominalis SASAKI 1,480, * Aphis gossypii GLOVER 867, * Macrosiphum avenae FABRICIUS 859, Cervaphis quercus TAKAHASHI 692, * Lipaphis erysimi KALTENBACH 645, Pleotrichophorus chrysanthemi THEOBALD 489, The above 10 species consisted $76.5\%$ of total catches and the 24 vector species consisted $55.5\%$. The curve of the seasonal occurrence of flying aphids at Horticultural Experiment Station shows bimodal, typical for the temperate region. The total number of trapped aphids at the Station from May to September, 1970, were less than that of average yearly catches at the College of Agriculture from 1967 to 1970. Thi, low numbers at Horticultural Experiment Station may attribute to the frequent spraying of insecticides from Spring to Summer on growing crops there. But the aphids population increase suddenly in the middle of October. This might be resulted from cease of insecticide applications and migration of aphids from summer host to winter host plants.

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Characterization of Spodoptera exigua Nuclear Polyhedrosis Virus Polyhedrin Gene Structure (파밤나방 핵다각체병 바이러스의 다각체 단백질 유전자 구조)

  • 최재영;김우진
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.144-149
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    • 1996
  • To develope the baculovirus expression vector system (BEVS) using Spodoptera exigua nuclear polyhedrosis virus (SeNPV), we characterized the polyhedrin of SeNPV. The SeNPV polyhedra was irregular and composed of the major protein molecular weight of 30 kDa determined by electronmicroscopy and SDS-AGE analysis, respectively. The nucleotid suquences of 876 bases including the coding region of polyhedrin gene was determined and it was revealed that the polyhedrin gene is located within Xho I 3.0Kb and Nco I 6.0 Kb by Southern blot analysis, respectively. Also, the Xho I 3.0 Kb and the Nco I 6.0 Kb fragments were cloned and restriction enzyme map of these clones were determined.

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