• Journal of the Korean Applied Science and Technology
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• v.38 no.2
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• pp.434-440
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• 2021

This study aimed to understand the effects on the improvement of hair texture by producing a hair texture improvement agent with milk thistle oil, and then applying it to the damaged hair. Each sample before and after application was measured. To understand the effects on the improvement of hair texture, the tensile strength, absorbance with the use of methylene blue, and gloss were measured. In the results of measuring the tensile strength to understand the effects on the improvement of hair texture, In the results of analyzing the absorbance with the use of methylene blue, the value after application was decreased than the value before application. In the results of measuring the gloss, none of the samples showed huge differences. In the results of this study, the milk thistle oil showed the effects on the texture improvement of damaged hair.

• Korean Journal of Pharmacognosy
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• v.51 no.4
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• pp.297-301
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• 2020

Silymarin (SM) and silybin diastereomers (SD) in milk thistle (Silybum marianum) were determined using high-performance liquid chromatography and quantified using a reverse-phase column in a gradient elution system. UV detection was performed at 288 nm. The content of SM and SD in milk thistle was 3.236 and 0.553 mg/g DW, respectively. Determining the presence and quantifying the content of SM and SD in milk thistle are vital for the pharmaceutical industry to identify optimal sources for developing health supplements or therapeutics.

• Journal of Food Hygiene and Safety
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• v.33 no.2
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• pp.124-130
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• 2018

The Ministry of Food and Drug Safety (MFDS) is amending its test methods for the use of health functional foods (dietary food supplement), in order to establish regulatory standards and specifications in Korea. In this regard, we continue to pursue and perform our research on the analytical method development for the items being researched and reviewed. In this study, we have developed a sensitive and selective test method that could simultaneously separate and determinate six major bioactive flavonolignans in silymarin, which are based on the use of a liquid chromatographic-tandem mass spectrometry (LC-MS/MS). The standard calibration curves presented a linearity effect with the correlation coefficient ($r^2$) > 0.999. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of $0.3{\sim}9.0{\mu}g/L$ and $0.8{\sim}27.3{\mu}g/L$, respectively. The recovery results ranged between 96.2~98.6% at 3 different concentration levels, and its relative standard deviations (RSDs) were less than 5% as noted in this study. The proposed analytical method was characterized with a noted high resolution of the individual silymarin constituents, and the assay was fully validated as well. Our research can provide a significant scientific evidence that can be useful to amend the silymarin test method for the Health Functional Food Code.