• Journal of Embryo Transfer
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• v.17 no.1
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• pp.61-65
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• 2002

This study was carried out to study the viability of oocytes when vitrified at various maturation stages. Bovine cumulus-oocyte complexes were recovered from ovaries at a slaughter and then divided into five groups: control group(unvitrified oocytes), 0 hr. group(composed of oocytes vitrified before the onset of maturation) and 10, 14, and 20 hrs groups(vitrified at 10, 14 and 20 hrs after the onset of maturation, respectively). The oocytes remained vitrified for 24 hrs, and then were thawed in 3$0^{\circ}C$. Survival and cleavage rates were investigated by results of in vitro culture and aceto-orcein staining or FDA test. No difference in the incidence of diploid oocytes was observed among the control, non-vitrified group(3.6%) and oocytes vitrified at 14 hrs(6.7%) or 20 hrs(1.7%). However, more diploid oocytes were detected after vitrification at 0 hr.(26.7%) and 10 hrs(21.7%) post maturation. The survival rate of all vitrified immature oocytes(12.0~38.0%) was low, 48.0% of unvitrified oocytes and oocytes vitrified before maturation or 0~ 10 hrs after the onset of maturation were higher than that of other groups. The overall fertilization and cleavage rates of vitrified immature oocytes (32.3 ~ 64.6% and 4.6 ~ 32.3%) were low, and 55.0% of unvitrified oocytes and the rate of immature oocytes were very higher than that of mature oocytes.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2001.11a
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• pp.46-46
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• 2001

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.54-54
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• 2002

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.05a
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• pp.162-162
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• 2002

• The Magazine for Energy Service Companies
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• s.14
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• pp.68-73
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• 2002

전미ESCO협회가 작성한 보고서에 따르면, 대부분의 국가의 경우, ESCO의 개념 자체가 새롭기 때문에 그 시장은 여전히 비교적 미성숙 단계이다. 그러나 산업, 상업 및 공공건물 영역 등에서 현저한 수준의 에너지 절감가능성 때문에 도입을 서두르고 있거나 이미 시작된 상태다. 이 보고서는 전미ESCO협회가 작성 중인 보고서에 관한 것으로 유럽 및 일본의 ESCO산업 동향을 파악하는 하나의 단서가 될 것이다

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.2
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• pp.112-119
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• 2012

This study investigated the diel and tidal distributions of the two mysids, Archaeomysis kokuboi and Acanthomysis nakazatoi, in relation to their growth stages in the sandy surf zone of Yongil Bay, located on the southeastern part of Korean Peninsula. Sampling was conducted with a sledge net at every two hours for almost 24 hours at three sites: water edge, water surface and sand bottom both in 1-m deep water areas. The abundance of Archaeomysis kokuboi juveniles was too low to count both in day and night samples. While there was no difference in immature A. kokuboi abundance between day and night in the bottom or water edge, that at the water surface was significantly higher at night than daytime. The abundance of A. kokuboi adults, especially of males, in the bottom was significantly higher in daytime than night and no individuals appeared to the water surface either day or night. In comparison, the abundance of Acanthomysis nakazatoi juveniles between day and night did not differ significantly at all the three sites, with the highest number being distributed in the bottom. The abundance of immatures between day and night also did not differ significantly and no individuals appeared to the water surface either day or night. The abundance of A. nakazatoi adults, especially females, in the bottom was significantly higher at night than daytime and there was no significant difference in abundance between day and night in the other sites. There was also no significant difference in abundances of the two species between ebb and flood tides, except for A. kokuboi immatures which appeared significantly more during the ebb tides at the water surface. Overall, the distribution of the two sympatric species, A. kokuboi and A. nakazatoi, was not the same in the sandy surf zone. Its difference seems to depend on their stages of growth, and the change in their abundance may be influenced more by diurnal rhythms than tidal effects. The population density of A. nakazatoi in the sandy surf zone was much higher than that of A. kokuboi, and relatively higher densities in all growth stages of the former were found in the sandy bottom ranging from juveniles to adults. These results indicate that A. nakazatoi has exceedingly better ability of sand burrowing even from the juvenile stage, and thus is an ecologically better adapted species in the sandy surf zone than another sympatric species, A. kokuboi.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.1
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• pp.47-55
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• 2017

This study evaluated critical factors influencing the success of regenerative endodontic treatment for necrotic immature permanent teeth. The study enrolled patients who had regenerative endodontic treatment in a necrotic immature permanent tooth through 1-year follow-up. Possible explanatory variables related to the success rates for 46 teeth were age, gender, dental stage at the initial appointment, etiology, treatment information, and clinical and radiographic outcomes. The dental stage at the time of regenerative endodontic treatment did not influence the success rate. The success rate was significantly related to the etiology of the necrotic immature tooth. The success rate according to etiology decreased in the following order: dens evaginatus, caries, and trauma. There was also a significant difference according to the type of antimicrobial. The success rate was influenced by appropriate disinfection of the root canal system, provision of a scaffold, and coronal sealing. Above all, careful case selection is necessary for treating a necrotic immature permanent tooth, especially in cases of trauma.

• Journal of Embryo Transfer
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• v.20 no.1
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• pp.63-69
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• 2005

This study were carried out to evaluate the possibility of nuclear progression of canine immature oocytes, of which was cultured in a reproductive tract, such as oviduct, ovarian bursa and uterus of estrus bitch for 4, 5 and 6 days following immediately collection. Cumulus intact oocytes(COC) fore collected from domestic dog following ovariohysterectomy at local veterinary clinics. In Exp. 1, COCs $of>110\;{\mu}m$ diameter were selected and cultured in vitro at $39^{\circ}C$, $5\%\;CO\_{2} $ in air atmosphere. The nuclear progression of canine oocytes checked at 24, 48 or 72 h after in vitro maturation. There was not increased the nuclear progression to the M II stage depending on culture periods at 24, 48 and 72h $(1.3\%,\;3.7\%\;and\;4.7\%)$. In Exp. 2, to evaluate of nuclear progression of immature oocytes, collected or in vitro cultured oocytes were transfer into a canine reproductive tract (oviduct, ovarian bursa and uterus). The recovery rates of canine oocytes from a reproductive tract after 4 days $(33.7\%)$ in vivo culture were significantly higher than those 5 $(17.7\%)$ 6 day $(3.4\%)$ (P<0.05). The survival rates of collected oocytes after 4 days $(60.0\%)$ were also significantly higher than those of 5 days $(30.2\%)$ and 6 days $(38.9\%)$ (P<0.05). The meiotic resumption rates of canine oocytes were not significantly difference among the culture periods at 4 days $(5.9\%)$, 5 days $(0.0\%)$ and 6 days $(0.0\%)$. These results show that the nuclear progression of canine immature oocytes from in in vivo culture was not affect the nuclear resumption of oocytes.

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.528-530
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• 2007

IODP Expedition 311에서 채취한 퇴적물 시료들의 TOC 함량은 대부분 1 %미만이고 육지에서 해안으로 갈수록 높은 함량을 보여준다. TOC/TN비는 4에서 10의 범위를 가지고 있으며, 이 값은 대부분의 유기물들이 육성식물 보다는 조류기원임을 보여준다. 반면에 Rock-Eval 분석 열분석결과는 유기물들이 Type III 의 육성식물 기원임을 지시하고, 대부분의 유기물들이 미성숙단계 (immature stage)에 있음을 보여준다. 유기물 기원에 대한 지화학적 지시자들 사이의 불일치를 해결하기위해 유기물의 탄소동위원소 ($^{13}C_{org}$) 분석을 실시하였다. 분석된 유기물 탄소동위원소 값은 -28.4 $%_o$,에서 -23.0 $%_o$의 범위를 가지고 있으며, 이는 유기물들이 식물성 기원보다는 해수 및 담수의 조류가 혼합된 기원이 우세하다는 j것을 지시해준다.

• Korean Journal of Plant Resources
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• v.26 no.5
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• pp.548-556
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• 2013

The objective of this study was to determine the effect of antioxidant enzyme activity and radical scavenging activities of cucumber grown greenhouse. The analytic method of antioxidant activities were measured by estimating DPPH free radical scavenging, nitrite scavenging ability activity and ABTS radical scavenging ability. The DPPH free radical scavenging activity of Jangjukcheongjang was the highest in most of the growth stage. The Nitrite scavenging ability at pH 1.2 was more than 40% in all cultivars and growth stages. The ABTS radical scavenging ability of Jangjukcheongjang and Nulpureuncheongjang showed relatively little higher than Janghyeongnakhap and Sinjoeunbaekdadagi. The SOD activity showed higher activity than 95% in all cultivars and growth stages. The activity of CAT was highest in the Nulpureuncheongjang of 2 (12~16cm) growth stage, and the APX activity of Nulpureuncheongjang and Janghyeongnakhap showed a relatively high activity. The POX activity showed distinctly different trends depending on the growth stage, and that is, the activity at harvest stage was significantly reduced. These results suggest that the cucumber had the potent biological activities, and that in the future, the availability of cucumber will be increase in the field of high-value cosmetic and food products.