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Influence of Stage of Maturation of Bovine Oocytes at Time of Vitrification on In Vitro Development and Viability  

김상근 (충남대학교 수의과대학)
신현주 (충남대학교 수의과대학)
Publication Information
Journal of Embryo Transfer / v.17, no.1, 2002 , pp. 61-65 More about this Journal
Abstract
This study was carried out to study the viability of oocytes when vitrified at various maturation stages. Bovine cumulus-oocyte complexes were recovered from ovaries at a slaughter and then divided into five groups: control group(unvitrified oocytes), 0 hr. group(composed of oocytes vitrified before the onset of maturation) and 10, 14, and 20 hrs groups(vitrified at 10, 14 and 20 hrs after the onset of maturation, respectively). The oocytes remained vitrified for 24 hrs, and then were thawed in 3$0^{\circ}C$. Survival and cleavage rates were investigated by results of in vitro culture and aceto-orcein staining or FDA test. No difference in the incidence of diploid oocytes was observed among the control, non-vitrified group(3.6%) and oocytes vitrified at 14 hrs(6.7%) or 20 hrs(1.7%). However, more diploid oocytes were detected after vitrification at 0 hr.(26.7%) and 10 hrs(21.7%) post maturation. The survival rate of all vitrified immature oocytes(12.0~38.0%) was low, 48.0% of unvitrified oocytes and oocytes vitrified before maturation or 0~ 10 hrs after the onset of maturation were higher than that of other groups. The overall fertilization and cleavage rates of vitrified immature oocytes (32.3 ~ 64.6% and 4.6 ~ 32.3%) were low, and 55.0% of unvitrified oocytes and the rate of immature oocytes were very higher than that of mature oocytes.
Keywords
embryos; vitrification; fertilization and cleavage rate;
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1 Caroll J, Wames GM and Matthews CD. 1989. Increase in digyny explains polyploidy after in vitro fertilization of frozen thawed mouse oocytes. J. Reprod. Fert., 85:489-494   DOI
2 Candy CJ, Wood M, Whittingham DG, Merriman JA and Choudhury N. 1994. Cryopreservation of immatur mouse oocytes. Hum. Reprod., 9:1738-1742   DOI
3 Kasai M, Komi JH, Takakamo A, Tssnoda H, Sakurai T and Machida T. 1990. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J. Reprod. Fertil., 89:91-97   DOI
4 Rall WF and Fahy GM. 1985. Ice-free cryopre-servation of mouse embryos at $-196^{\circ}C$ by vitrification. Nature, 313:573-575   DOI   ScienceOn
5 Tachikawa S, Otoi T, Kondo S, Machida T and Kasai M. 1993. Successful vitrification of bovine blastocyst, derived by in vitro maturation and fertilization. Mol. Reprod. Dev., 34:266-271   DOI   ScienceOn
6 van Blerkom J. 1989. Maturation of high frequency of germinal vesicle-stage mouse oocytes in mice. Theriogenology, 33; 365   DOI   ScienceOn
7 Shaw PW, Benarde AG, Fuller BJ, Hunter JH and Shaw RW. 1992. Vitrification of oocytes using short cryoprotectant exposure : Effects of vary-ing exposure times on survival. Mol. Reprod. Devlop., 33:210-214   DOI   ScienceOn
8 van der Elst JC, Nerinckx SS and van Steirteghem AC. 1993. Slow and ultrarapid freezing of fully grown germinal vesicle-stage mouse oocytes: optimization of survival rate outweighted by defective blastocyst formation. J. Assist. Reprod. Gene, 10: 202-212   DOI   ScienceOn
9 Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen A, Greve T and Callesen H. 1998. Open pulled straw(OPS) vitrification : a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Dev., 51:53-58   DOI   ScienceOn
10 Luna HS, Ferrari I and Rumpf R. 2001. influence of stage of maturation of bovine oocytes at time of vitrification on the incidence of diploid metaphase II at completionof maturation. Anim. Reprod. Sci., 68:23-28   DOI   ScienceOn
11 Rall WF and Wood MJ. 1994. High in vitro and in vivo survival of day 3 mouse embryos vitrified or frozen in a non-toxic solution of glycerol and albumin. J. Reprod. Fertil., 101:681-688   DOI
12 Toth TL, Jones HW, Baka SC, Muasher S, Veeck LL and Lanzendorf SE. 1994. Fertilization and in vitro development of cryopreserved human prophase I oocytes. Fertil. Steril., 61: 891-894   DOI
13 Leibo SP and Oda K. 1993. High survival of mouse zygote and embryos cooled rapidly or slowly in ethylene glycol plus polyvinylpyrrolidone. Cryo-Letters, 14:133-144
14 Rubinski B, Arav A and Devires AL. 1991 Cryopreservation of oocytes using directional cooling and antifreeze glycoproteins. Cryo-Letters, 12:93-106
15 Schilling E, Niemann H and Smidt D. 1982. Evaluation of fresh and frozen cattle embryos by fluorescence microscopy. Cryobio., 15:245-248   DOI   PUBMED   ScienceOn
16 Suzuki T and Nishikata Y. 1992. Fertilization and cleavage of frozen thawed bovine oocytes by one step dilution method in vitro. Therio-genology, 37:306   DOI   ScienceOn
17 Kon T, Kwon OY and Nakahara T. 1991. Develo-pment of vitirfied mouse oocytes after in vitro fertilization. Cryobiology, 28:50-54   DOI   ScienceOn
18 Dinnyes A, Wallace GA and Rall WF. 1995. Effect of genotype on the efficiency of mouse embryo cryopreservation by vitrification of slow freezing methods. Mol. Reprod. Dev., 40:429-435   DOI   ScienceOn