• Title/Summary/Keyword: 미생물 총 균수

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Physicochemical and Microbial Properties of Korean Traditional Rice Wine, Makgeolli, Supplemented with Mulberry during Fermentation (오디를 첨가한 막걸리의 발효기간 중 이화학적 및 미생물학적 특성)

  • Kim, Eunkyung;Chang, Yoon Hyuk;Ko, Jae Youn;Jeong, Yoonhwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.10
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    • pp.1682-1689
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    • 2013
  • The objective of the present study is to elucidate the physicochemical and microbial properties of Korean traditional rice wine, Makgeolli, supplemented with mulberry during the fermentation process. Four hundred grams of mulberries (20% of the total amount of rice) were ground and incorporated into the rice solution (3.5 L of distilled water, 2.0 kg of rice, 28.0 g of yeast, and 80.0 g of nuruk). The rice solution was then placed in a water bath set at $28^{\circ}C$ and left to ferment for 7 days. The alcohol contents between the control and mulberry Makgeolli were not significantly different. The pH values of the control and mulberry Makgeolli decreased and the total acidity values were elevated after a 7-day fermentation. In the microbial analysis, the total viable cell count, lactic acid bacteria count and yeast count increased considerably through the 7-day fermentation process. For all the samples, the glucose and succinic acid contents were highest among all free sugars and organic acids, respectively. Forty-eight different volatile compounds were found in all the samples after the 7-day fermentation process.

Quality Characteristics of Kimchi Prepared for the Winter around Chonnam Area (전남지역 김장 배추김치의 품질 특성)

  • 박복희;조희숙;유맹자
    • Korean Journal of Human Ecology
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    • v.6 no.2
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    • pp.57-65
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    • 2003
  • To investigate quality characteristics of kimchi prepared for the winter around Chonnam area, home made kimchi samples collected from 22 area, and they were stored at -1${\pm}$1$^{\circ}C$. The results were as follows : The pH and acidity of kimchi samples were 4.75 and 0.84%. respectively. Salt concentration was 3.50% and in Redox potential measurement, Eh value was -134.08mV. Ascorbic acid and reducing sugar contents were 10.l8mg% and 13.25mg%, respectively, In color measurement, L value was 52.29 and a and b values were 19.68 and 27.69, respectively. Total viable count was 5.5${\times}$10$\^$6/ and lactic acid bacteria count and yeast were 4.6${\times}$10$\^$5/ and 8.8${\times}$10$\^$5/. respectively. Properties of hardness of kimchi measured instrumentally was 9.26kgf. Alcohol insoluble solids(AIS) content was 5.53% and hot water soluble pectin(HWSP) content and sodium hexametaphosphate soluble pectin(NaSP) content were 17.35% and 29.65%, respectively, also hydrochloric acid soluble pectin(HClSP) content was 53.0%.

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Quality Properties of Kimch by the Addition of King Oyster Mushroom(Pleurotus eryngii) during Fermentation (큰느타리버섯 첨가량에 따른 김치의 품질 특성)

  • Kim Jeong-Han;Jang Myoung-Jun;Choi Jong-In;Ha Tae-Moon;Chung Jae-Woon;Chi Jeong-Hyun;Ju Young-Cheoul
    • Food Science and Preservation
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    • v.12 no.3
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    • pp.287-291
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    • 2005
  • Quality properties of Kimchi added with king oyster mushroom(Pleurotus eryngil) was evaluated during the fermentation at $5^{\circ}C$. Until 7 days, pH of Kimdhi was rapidly decreased. The titratable acidity of Kimchi was in inverse state to the pH. Kimchi added with the mushroom showed a lower pH with a higher titratable acidity than those of control. Total microbial load and lactic acid bacterial count were maximized at 18 days, thereafter slowly decreased. king oyster mushroom Kimchi added with showed an antioxidant activity. The effect was dependent fo the amount of much room, and radical scavenging activity and content of total phenolic compounds was the highest in eddition of $30\%$ king oyster mushroom.

Assessment of Bacterial Contamination of Raw Meats Sold in Korea, 2007 (2007년 한국에서 판매된 식육의 미생물 오염도 평가)

  • Lee, Do-Kyung;Hwang, Jae-Woong;Yang, Hwan-Jin;Jang, Soek;Baek, Eun-Hye;Kim, Mi-Jin;Kim, Jung-Hyun;Lee, Sang-Jin;Ha, Nam-Joo
    • Environmental Analysis Health and Toxicology
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    • v.22 no.4
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    • pp.313-320
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    • 2007
  • 본 연구는 인간의 건강과 식품의 오염을 예방하기 위한 유용한 자료를 얻기 위하여 육류(소고기, 닭고기, 돼지고기)로부터 분리된 staphylococci를 가지고 한국에서 광범위하게 사용되는 6가지 항생제와 daptomycin, linezolid, quinupristin/dalfopristin and tigecycline과 같은 4가지의 신항생제에 대한 항생제 감수성 시험을 수행하였다. 이에 더하여 육류의 세균 오염실태를 조사하기 위하여 총 세균수와 대장균수를 측정하였다. 그 결과 147개의 육류 샘플 중 48%의 샘플에서 일반세균수의 기준에 부적합한 결과가 나타났으며, 대장균수에 대해서는 31%의 샘플에서 기준에 부적합한 결과가 나타났다. 또한 육류의 91%에서 stapylococci가 검출되었으며 이들 staphylococci는 Coagulase Negative Staphylococci (CNS)와 S. aureus로 동정되었다. 분리된 staphylococci의 5.4%가 methicillin (oxacillin)에 내성을 나타내었고, 특히 mupirocin ($MIC_{90},\;>\;12\;{\mu}g/mL$) gentamicin ($MIC_{90},\;64\;{\mu}g/mL$)에 높은 내성을 나타내었다. 그러나 신항생제에 대해서는 methicillin 내성 staphylococci를 포함한 모든 staphylococci 분리균주가 높은 감수성을 나타내었다. 결론적으로 본 연구의 결과들은 육류가 소비자들의 건강에 중요한 위험요소로 작용할 수 있음을 보여주고 있다.

Quality Characteristics of Kimchi Added with Allium hookeri Root (삼채뿌리를 첨가한 김치의 품질 특성)

  • You, Bo Ram;Kim, Hyun Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.10
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    • pp.1649-1655
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    • 2013
  • This study is conducted to investigate the quality characteristics of kimchi with added Allium hookeri root (AHR) during a 56-day fermentation process at $4^{\circ}C$. AHR was added to salted cabbage at concentrations of 0, 5, 10, and 20% (w/w). The quality characteristics of the kimchi with added AHR were determined by measuring pH, acidity, salinity, reducing sugar, microbial amounts, and sensory evaluation. AHR had a higher level of crude lipid and potassium than other kinds within the Allium family. All kimchi with added AHR gradually decreased in the pH level compared to Baechu kimchi until 2 weeks, and kept a higher level of pH than Baechu kimchi until 8 weeks. Salinity showed a range of 1.87~2.43% over 8 weeks. The reducing sugar content showed no difference between all kimchi. In sensory evaluations, overall acceptance, taste and texture were highest in kimchi with added 10% AHR.

The Effects of Added Sesame Powder on the Quality of Baechukimchi (참깨가루의 첨가가 배추김치의 품질에 미치는 영향)

  • Moon, Sung-Won;Lee, Myung-Ki
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.1
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    • pp.52-61
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    • 2009
  • In this study, we evaluated the effects of sesame powder on the fermentation of Baechukimchi, by assessing sensory, physicochemical, and microbiological properties during up to 25 days of fermentation. The Baechukimchi, with various levels [0, 1, 2, 3, 4%(w/w)] of sesame powder, was fermented at $10^{\circ}C$. The product containing the control and 1% sesame powder evidenced the highest scores for appearance and smell. Taste and texture were highest in the 1% and 2% sesame powder, and the overall acceptability was highest in the 2% sesame powder sample. During fermentation, titratable acidity increased while pH gradually decreased. pH was higher in the sample with sesame powder than in the control, and the titratable acidity increased with increasing sesame powder content on day O. Reducing sugar increased sharply during fermentation, and then gradually decreased. In particular, the 2% sesame powder sample maintained the highest content. Total vitamin C was slightly increased at initial fermentation and then steadily decreased. The total polyphenol content and antioxidant effect of the experimental groups with added sesame powder were higher than those of the controls. Additionally, the time required to achieve maximum levels of lactic acid bacteria, as determined by log numbers of cells and total viable cells, were more delayed in the experimental groups with added sesame powder than in the controls. Our results indicated that the Baechukimchi with $1{\sim}2%$ added sesame powder was acceptable.

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Seasonal, regional distribution and identification of psychrotrophic bacteria in milk (원유 내 내냉성 미생물의 계절별, 지역별 분포 및 동정)

  • Shin, Yong Kook;Lee, Hyun Ah;Oh, Nam Su;Nam, Myoung Soo
    • Korean Journal of Agricultural Science
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    • v.40 no.1
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    • pp.27-34
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    • 2013
  • To investigate the distribution of psychrotrophic bacteria, raw milk was collected from farms in nine different regions located around Kyunggi province in South Korea at four different seasons. Psychrotrophic counts were higher in winter than in other seasons as $3.0{\times}10^4$ CFU/mL (p<0.05). Among nine regions, the population in raw milk sampled from B region was in significantly greater numbers and those from C and D province were in significantly lower numbers than any other regions, $2.4{\times}10^5$ CFU/mL and $8.7{\times}10^3$ CFU/mL, respectively (p<0.05). In addition, among 706 bacterial isolates, the predominant class was Gamma-proteobacteria (81.02%) and genus was Pseudomonas (32.34%), especially Pseudomonas fluorescens (39.46%). Compared to the regional predominance, Acinetobacter johnsonii in A region, Pseudomonas fluorescens in B region, Enterobacter amnigenus in C region, Psychrobacter maritimus in D region, Acinetobacter johnsonii in E region, Acinetobacter haemolyticus in F region, Pseudomonas fluorescens in G region, Acinetobacter jounsonii in H region, and Pseudomonas mucidolens in I region were found.

Anti-Helicobacter pylori Activity of Yogurt Fermented with Lactic Acid Bacteria from Baikkimchi (백김치 유래 유산균을 이용한 요구르트의 Anti-Helicobacter pylori 활성)

  • Lim, Sung-Mee;Kim, Duck-Sool;Ahn, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.334-344
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    • 2014
  • The objective of this study was to evaluate the microbiological and physicochemical characteristics, and the antagonistic activity against Helicobacter pylori ATCC 43504, of yogurt fermented with the lactic acid bacteria from Baikkimchi kept under cold storage. The viable cell counts, titratable acidity, viscosity, and total solid content of the yogurt were different according to the bacterial strains used for fermentation. There was no significant change (P>0.05) in the various properties of refrigerated yogurt. Among the tested strains, the strongest resistance against artificial gastric juice and bile salt was found for Lactobacillus brevis BK11 and Lactobacillus paracasei BK57. Due to high lactic acid levels obtained from these two lactic acid bacteria, yogurt may show good anti-Helicobacter effects according to the time-kill assay. In particular, yogurt fermented with L. brevis BK11 significantly reduced the number of H. pylori adhering to gastric epithelial AGS cells and the urease activity of this pathogen (P<0.05).

Comparison of the quality Characteristics of Brown rice Glutinous rice Gochujang with different Enzymes (효소제를 달리한 현미 찹쌀 고추장의 품질특성 비교)

  • Jo, Ha-Yeong;Hong, Jae-Hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.4
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    • pp.49-55
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    • 2021
  • The purpose of this study was to compare the quality characteristics of each brown rice glutinous rice red pepper paste prepared. In this study, brown rice, glutinous rice, and red pepper paste were prepared with various enzyme preparations. A 20-day study of the physicochemical and microbiological properties of each enzyme through the results process showed significant differences. In particular, brown rice red pepper paste manufactured using cozies purchased from Sunchang was functionally superior to rice cozies and bean cozies, with probiotics of 7.1-7.5×107 CFU/mL, lactobacillus of 1.9-2.2×103 CFU/mL, 48.67-52.30%, and 58.57-63.90%. When the results were combined, the composition of microorganisms and enzymes of fermented soybean paste purchased from Sunchang was not confirmed, but brown rice chili paste with nutritional and functional properties could be prepared compared to A.oryzae and A. kawachii.

Effect of Crab Shell on Shelf-life Enhancement of Kimchi (게껍질의 김치보존성 향상효과)

  • 김순동;김미향;김일두
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.6
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    • pp.907-914
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    • 1996
  • To enhance the shelf-life and quality of baechu kimchi, the effects of CSP(crab shell powder) addition to kimchi was investigated. Overall qualities were deteriorated by fish odor, chewiness of particles, sharp pH increase at the early fermentation stage; therefore in order to solve these problems kimchi fermentation was carried out with kimchi containing 1, 3, 5% CSPB for salted baech weight at $10^{\circ}C$ for 300ays. Quality of kimchi was evalutated by the measurement of pH, acidity, colour L, a, and b value, the number of microbe and lactic acid bacteria, texture. Ten highly trained panelists were involved in the sensory evaluation. During the entire fermentation periods, pH, hardness, colour L, a and b value, the number of lactic acid bacteria of kimchi with CSPB were higher than those of control, but acidity was lower. Sensory quality showed that sour taste of control at 15-day fermentation was already strong. However, sour taste, crispness taste, and overall taste of kimchi with CSPB untill 20-day fermentation were good. Especially, overall taste of kimchi containing 3% CSPB at 30-day fermentation was good, but that of kimchi containing 5% showed fish odor from the early periods of fermentation.

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