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http://dx.doi.org/10.7744/cnujas.2013.40.1.027

Seasonal, regional distribution and identification of psychrotrophic bacteria in milk  

Shin, Yong Kook (R&D Center, Seoul Dairy Cooperative)
Lee, Hyun Ah (R&D Center, Seoul Dairy Cooperative)
Oh, Nam Su (R&D Center, Seoul Dairy Cooperative)
Nam, Myoung Soo (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.40, no.1, 2013 , pp. 27-34 More about this Journal
Abstract
To investigate the distribution of psychrotrophic bacteria, raw milk was collected from farms in nine different regions located around Kyunggi province in South Korea at four different seasons. Psychrotrophic counts were higher in winter than in other seasons as $3.0{\times}10^4$ CFU/mL (p<0.05). Among nine regions, the population in raw milk sampled from B region was in significantly greater numbers and those from C and D province were in significantly lower numbers than any other regions, $2.4{\times}10^5$ CFU/mL and $8.7{\times}10^3$ CFU/mL, respectively (p<0.05). In addition, among 706 bacterial isolates, the predominant class was Gamma-proteobacteria (81.02%) and genus was Pseudomonas (32.34%), especially Pseudomonas fluorescens (39.46%). Compared to the regional predominance, Acinetobacter johnsonii in A region, Pseudomonas fluorescens in B region, Enterobacter amnigenus in C region, Psychrobacter maritimus in D region, Acinetobacter johnsonii in E region, Acinetobacter haemolyticus in F region, Pseudomonas fluorescens in G region, Acinetobacter jounsonii in H region, and Pseudomonas mucidolens in I region were found.
Keywords
Psychrotrophic bacteria; Raw milk; Gamma-proteobacteria; Pseudomonas fluorescens;
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