• Korean Journal of Microbiology
• /
• v.51 no.4
• /
• pp.427-434
• /
• 2015

Most of halophilic archaea are found in the various hypersaline environments including solar saltern, salt lake with very high salt concentration. The present study is about isolation and characterization of halphilic archaea from Gomso solar saltern known as a representative high salt environment in Korea. In order to isolate the halophilic archaea, we prepared and used high salt medium. Finally, total 7 strains obtained were tentatively identified based on comparative similarity analysis for 16S rRNA gene sequence and physiological traits. All halophilic archaea belonged to Haloruburm, Halogeometriucm, Halobacterium, and Haloarcula genera. These isolates were all Gram-staining negative, and growth was not observed using nitrate as an alternative electron acceptor under anaerobic conditions. In addition, all isolates required about 12-30% (w/v, NaCl) salt. This case study might provide basic information on microbial isolation technologies and related research in halophilic microorganisms from domestic halophilic environments, and contribute to obtaining useful indigenous halophilic archaea in a variety of extreme environmental conditions.

• Proceedings of the KAIS Fall Conference
• /
• 2005.05a
• /
• pp.277-278
• /
• 2005

미생물 고정화를 위하여 PU 폼을 세 가지 처리 방법으로 처리하였다. 플라즈마 처리, 플라즈마 유도 그라프팅, 플라즈마 유도 그라프팅 후 플라즈마로 재처리 방법을 사용하였다. 플라즈마 처리는 bacteria adhesion에 큰 효과를 주지 못하였고, 아크릴산의 플라즈마 유도 그라프팅은 adhesion을 세 배 이상 증가시켰다. Bacteria의 adhesion 성능 향상은 표면의 친수성을 증가시키고 새로운 groove나 cavity들을 형성시킴으로 가능하였다. 반면, 그라프팅 후 plasma re-treatment는 미생물 고정화를 크게 향상시키지 못하였다. SEM 관찰을 통하여 대부분의 E.coli.는 groove나 cavities라는 shear-free area에 서식함을 알 수 있었다. PU폼의 bacteria adhesion은 미생물과 고분자 표면 사이의 정전기적 인력이나 van der Waals 인 력에 의해 주로 영향을 받는다고 사료된다.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2007.11a
• /
• pp.262-263
• /
• 2007

유전체학 (genomics)의 가장 기초적인 기반이 되는 것은 염기서열을 정확하게 결정해 내는 것이다. 많은 진핵생물들 (eukaryotes)은 두개의 상동염색체를 가지며 두개의 염색체의 염기서열에는 차이가 존재한다. 현재의 유전체 염기서열 결정방법으로는 염기변이가 많이 존재할 경우 유전체의 염기서열을 결정하기 어렵다. 특정한 장소에 서식하는 무수히 많은 미생물들의 유전체의 염기서열을 동시에 결정하는 문제도 미생물학에서 중요성을 인정받는 문제이지만, 미생물들간의 염기변이의 정도는 단일개체의 경우보다 복잡하며 염기서열을 효과적으로 결정하기 힘들다. 따라서 염기변이가 많은 생물들과 미생물들 집합의 염기서열을 결정할 수 있는 방법론의 개발이 시급한 실정이다. 본 논문에서는 조립된 다염기변이 유전체및 메타유전체의 염기서열의 정확성을 평가하기 위한 유전체 서열과 시뮬레이션에 기반한 read 들을 생성하는 도구를 개발하는 것을 목표로 한다.

• Proceedings of the Speleological Society Conference
• /
• 2002.11a
• /
• pp.31-38
• /
• 2002

동굴생물은 식물과 동굴, 미생물로 크게 구분되지만 태양광선이 완전히 차단된 동굴내 환경에서는 동굴동물들이 주종을 이루고 있다(임, 1985). 동굴내의 동물에 관한 연구는 1966년 고씨동굴, 용담굴을 시발로 고수굴, 천동굴, 노동굴, 백룡굴, 대이굴, 환선굴 등 충청북도와 강원도일대에 분포하는 동굴의 생물상이 조사되었다. (중략)

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2003.04a
• /
• pp.66-71
• /
• 2003

오늘날 인간 생활에서 섬유제품은 인체와 가장 많이 접하고 있어서 인체의 분비물과 함께 미생물이 서식하여 인체 건강을 해치거나 악취를 발생시킨다. 이러한 현상은 섬유제품을 변색, 오염, 손상시키므로 항균, 소취성있는 섬유제품이 요구된다. 또한 섬유제품에 처리된 항균, 소취가공제는 안전성이 요구되며, 동시에 자연계에서 간단히 분해되고 유해물질을 생성하지 않아야 한다. (중략)

• Proceedings of the Speleological Society Conference
• /
• 2002.07a
• /
• pp.31-41
• /
• 2002

지상의 생태계는 광합성을 통해 광 Energy를 화학 Energy로 전환하는 생산자인 녹색식물들과 이들을 먹고사는 1차 소비자인 초식동물 또 이들을 먹고사는 육식동물인 2차 소비자와 동식물의 사체나 유기물을 분해하며 살아가는 미생물 같은 분해자로 구성되어 있다. 대부분의 생태계는 태양광선을 Energy원으로 하여 풍부한 물질생산과 먹이사슬을 통하여 안정적인 영양공급이 이루어지고 있다. (중략)

• Research in Plant Disease
• /
• v.26 no.4
• /
• pp.210-221
• /
• 2020

This research was executed to select beneficial antagonists from digestive organ of Allomyrina dichotoma larva that can be put on environment friendly control against phytopathogenic fungi. We screened 38 bacterial strains inhibiting mycelial growth against eight plant pathogens through dual culture assay. The 10 strains among 38 bacterial strains were selected as beneficial microbes showing antifungal activity against Botrytis cinerea, Plasmodiophora brassicae, Colletotrichum acutatum and Phytophthora capsici through under greenhouse pot trials. The 10 bacterial strains that shown strongest antifungal activity were classified into 3 genera and 10 species, and identified as the genus Bacillus (DM146, DM152, DH2, and DH16), Paenibacillus (DF30, DH14, and DM142) and Streptomyces (DF137, DM48, and DH92) by morphological characteristics and 16s rRNA gene sequence. The 10 bacterial strains had solubilizing activity of insoluble phosphates, production of IAA (indole-3-acetic acid), β-1,3-glucanase and protease. Among the 10 bacterial strains, DM152 strain was produced significant enhancement of all growth parameters of chili pepper and tomato seedlings under greenhouse condition. Thus, this study demonstrated that gut microbes of Allomyrina dichotoma larva will be useful as a potential biocontrol agent against plant pathogens and biofertilizer.

• Microbiology and Biotechnology Letters
• /
• v.7 no.3
• /
• pp.175-179
• /
• 1979

The present work was conducted in order to survey bacterial flora inhabiting in Crassostrea gigas. The Crassostrea gigas, sea water and mud of its living environments were sampled at Goseong bay, Gyungnam and Dolsan, Yeochon-gun, Chungnam at the period of from December 1978 to February 1979. The obtained results are summarized as follows: 1. The range of bacterial counts found in Crassostrea gigas, sea water and mud was respectively 10$^4$~10$^{6}$ , 10$^{5}$ ~10$^{6}$ and 10$^3$~10$^4$. 2. Among the bacterial 382 strains isolated from the those sample sources, Vibrio genus was 43.5％, Pseudomonas 16.7％, Moraxella 11.5％ and Flavobacterium-cytophaga 8.9％. 3. Among 173 strains classified as Vibrio genus, 56％ of them was found in Crassostrea gigas, 54％ in mud and 25％ in sea water. 4. Pseudomonas type I was found only in sea water and type III/IV found in sea water, mud and Crassostrea gigas. Especially type III/IV was found much in sea water.

• Korean Journal of Microbiology
• /
• v.46 no.3
• /
• pp.255-261
• /
• 2010

The bacterial community structure associated with two marine sponges, Hyrtios sp. 604 and Callyspongia sp. 612 collected from the South Pacific Ocean were analyzed by 16S rDNA-denaturing gradient gel electrophoresis (DGGE). The phylogenetic analysis showed that the bacterial community associated with Hyrtios sp. 604 contained diverse bacterial groups such as Chloroflexi, Firmicutes, Cyanobacteria, Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, and Acidobacteria. Callyspongia sp. 612 harbored Chloroflexi, Cyanobacteria, Alphaproteobacteria, and Gammaproteobacteria. Hyrtios sp. 604 belonging to genus Hyrtios known to produce natural products showed greater bacterial diversity than Callyspongia sp. 612. Phylum Actinobacteria was shown to be one of dominant bacterial groups in Hyrtios sp. 604. Although the same phyla of bacteria were found in both sponge species, the spongeassociated predominant bacterial groups differed between the two sponges with different chemical characteristics from the same geographical location. Uncultured bacteria represented over 90% of the bacteria diversity present in all bacterial communities of the sponges.

• Korean Journal of Microbiology
• /
• v.55 no.1
• /
• pp.17-24
• /
• 2019

Bacteria in the polar region are under strong light and ultraviolet radiation. In this study, we investigated the effects of light on the growth of a psychrophilic bacterium, Sphingomonas sp. PAMC 26621, isolated from an Arctic lichen Cetraria sp. The growth of the strain in the light was lower than that in the dark. Surprisingly, thiamine increased the growth of Sphingomonas sp. PAMC 26621 in M9 minimal medium under light conditions. Thiamine increased the growth of the strain in a concentration-dependent manner along with ascorbic acid. N-acetylcysteine had no effect on the growth of the strain in the light. Thiamine and ascorbic acid also increased the activities of glucose-6-phosphate dehydrogenase and superoxide dismutase. The results of this study indicate that thiamine provided by the lichen symbiosis system plays an important role in light-induced oxidative stress in this Arctic bacterium as an antioxidant. Our study provide insight into the biochemistry and physiology of Arctic bacteria under strong light and ultraviolet radiation.