• Title/Summary/Keyword: 미생물 생육

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특수목적식품 제조용 프레스햄의 위생화를 위한 제조공정 단계별 감마선 조사기술 적용 : 미생물학적 평가

  • Byeon, Ui-Baek;Lee, Ju-Un;Kim, Jae-Hun;O, Sang-Hui;Seo, Ji-Hyeon;Kim, Jang-Ho;Byeon, Myeong-U
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2005.05a
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    • pp.282-284
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    • 2005
  • 본 연구는 특수목적식품을 개발하기 위한 대표적인 비가열 살균처리 기술인 방사선 살균기술을 이용하여 저장 중 프레스햄의 미생물학적 품질을 평가하고자 하였다. 그 결과 감마선비조사구의 경우 제조공정 단계에 관계없이 $30^{\circ}C$ 저장 4일 째에 모두 미생물 생육이 활발해져 장기저장에 부적합한 것으로 나타났으나, 감마선을 조사할 경우 훈연후 및 살균전 시료의 경우 5 kGy, 살균후 시료의 경우 3 kGy의 감마선 조사로 $30^{\circ}C$ 저장 4일 까지 미생물의 생육이 검출되지 않았다. 따라서, 감마선 조사기술은 프레스햄의 살균방법으로 이용이 가능하며, 특히 장기저장을 목적으로 하는 우주식, 환자식, 전투식량 등의 특수목적식품의 개발에 그 이용이 가능하다 할 수 있다.

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Effect of Liquid Fertilizer Contained Medium of Lactobacillus sp. and Saccharomyces sp. on Growth of Creeping Bentgrass (유산균과 효모균 배양액 함유 액비 시용이 크리핑 벤트그래스의 생육에 미치는 영향)

  • Kim, Young-Sun;Ham, Suon-Kyu;Lee, Sang-Jin
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.138-144
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    • 2010
  • This study was conducted to investigate the effect of Lactobacillus sp. and Saccharomyces sp. on turf quality, shoot and root growth of creeping betgrass in golf course by measuring turf color index, chlorophyll content, dry weight of shoot and root, T/R ratio and root length. Fertilizer treatment was designed as follows; nonfertilizer (NF), control (CF; compound fertilizer), microorganism medium(MO; CF+MO)), microorganism medium contained Fe(MO-Fe; CF+MO-Fe) and microorganisum medium contained S (MO-S; CF+MO-S). Soil properties investigated after experiment was scarcely affected by applied fertilizers in root zone of creeping bentgrass. The turf color index and chlorophyll index of MO, MO-Fe, MO-S treatment were higher than those of NF, and similar to those of CF. The turfgrass root in MO and MO-Fe treatment was longer than others. The dry weight of shoot in MO and MO-S was higher than CF and that of root in MO and MO-Fe, and dry weight of MO was increased than that of NF and CF, by 26% and 6%, respectively. AS compared with NF, T/R ratio of CF, MO, MO-Fe and MO-S was increased, and MO and MO-Fe was similar to CF, MO-S higher. Nutrient content in CF, MO, MO-Fe and MO-S was contained more than in NF, and it was higher in shoot. These was suggested that application of MO induced the development of quality and growth of creeping bentgrass by assisting root growth and nutrients uptake.

Effects of Some Environment-friendly Farming Materials on Growth of Pepper and Tomato Seedlings under Low Solar Radiation Conditions (저일조 조건에서 몇 가지 친환경농자재가 고추와 토마토의 유묘 생육에 미치는 영향)

  • Um, Yeong-Cheol;Seo, Tae-Cheol;Jang, Yoon-Ah;Lee, Sang-Gyu;Lee, Jun-Gu;Choi, Chang-Sun;Oh, Sang-Seok
    • Journal of Bio-Environment Control
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    • v.20 no.4
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    • pp.269-276
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    • 2011
  • The aim of this study was to evaluate the effect of 3 environment- friendly farming materials on the growth of pepper and tomato seedlings under low level of solar radiation. The pepper and tomato seedlings were separately grown on commercial substrate and non-fertilizer substrate under 30% shading condition, respectively. The microbe-, enzyme-, and amino acid-supplements were used for the foliar application to the seedlings. The application of enzyme- and amino acid-supplements significantly enhanced the growth of tomato and pepper seedlings grown on non-fertilizer substrate, while there was no significant effect on the seedlings grown on commercial substrate. The foliar application of enzyme- and amino acid-supplements reduced the days to flowering by 3 to 4 days and lowered the node order of fruit set in tomato plants grown on non-fertilizer substrate. The results showed that the enzyme- and amino acid-supplements could enhance the seedling growth and lower the node order of fruit set under limited nutritional conditions.

Biocontrol of Vegetables Damping-off by Bacillis ehimensis YJ-37 (Bacillus ehimensis YJ-37에 의한 채소류 모잘록병의 생물학적 방제)

  • 김진호;최용화;강상재;이인구;주길재
    • Journal of Life Science
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    • v.12 no.4
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    • pp.416-422
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    • 2002
  • Bacillus ehimensis YJ-37 was observed as a potential biological agent to control the occurrence of diseases and plant growth.promoting rhizobacteria (PGPR). Population density of B. ehimensis YJ-37 were higher 1.2~2 times in main roots and lateral roots than from nonrhizosphere soil and persisted around 10$^4$g root on the watermelon and radish root system upto 30 days after growing in pot condition. As a PGPR, B. ehimensis YJ-37 enhanced plant growth of watermelon and radish by soil treatment. The leaf area, hypocotyl length, root length and dry weight of radish were about 85, 33, 23 and 89% more than that of untreated plant, respectively. In case of watermelon were about 63, 27, 25 and 69% more than that of untreated plant, respectively. Biocontrol of damping-off in watermelon and radish caused by Rhizoctonia solani AG-4 and Pythium ultimum were carried out in pots using 3. ehimensis YJ-37. The results showed that might contribute to it's suppression of damping-off disease in field plants.

Growth Effect of Tomato Treated with Bacillus sp. WRD-1 Cultures (Bacillus sp. WRD-1 배양액 처리가 토마토 생육에 미치는 영향)

  • Ok, Min;Seo, Won-Seok;Bae, Kye-Sun;Kwon, O-Chang;Park, Su-Jin;Cho, Young-Su
    • Korean Journal of Environmental Agriculture
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    • v.20 no.1
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    • pp.63-66
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    • 2001
  • To investgate growth effect of tomato by Bacillus sp. WRD-1 isolated from soil, the Bacillus sp. WRD-1 cultures were treated into tomato cultivated soil with different dilutions (1:100, 1:300, and 1:500) and autoclaved Bacillus cultures as control. Growth and yeild of tomato enhanced in treatments of the Bacillus cultures compared to control. The populations of native bacteria and actinomyces were increased twice in field treated with Bacillus sp. WRD-1 cultures, but the number of mold was decreased. Since the Bacillus sp. WRD-1 promoted growth of tomato and affected population dynamics of microorganism in field, this strain is prominent candidate as a microbial biocide to improve soil potential.

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Growth Promotion of Lettuce by Biofertilizer, BIOACTIVE, Prepared from Bacillus subtilus HR-1019 and N-acetyl-thioproline (Bacillus subtilus HR-1019와 N-Acetyl-thioproline으로 제조한 미생물처리제, BIOACTIVE에 의한 상추의 생장 촉진)

  • Lee, Yong-Suk;Park, Dong-Ju;Kim, Jae Hoon;Kim, Hyeong Seok;Chung, Soo Yeol;Choi, Yong-Lark
    • Journal of Life Science
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    • v.23 no.1
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    • pp.79-83
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    • 2013
  • A biofertilizer, BIOACTIVE, was manufactured by N-acetyl-thioproline (ATCA) and mineral phosphate solubilizing bacteria. The growth promoting effect of the biofertilizer on lettuce was evaluated under three different pot conditions, and its stability was assessed in the field. According to the results of the pot experiments, plant growth was improved compared with that of control: 128%, 122%, and 153% for the leaf number, leaf length, and leaf mass, respectively. Applying the manufactured biofertilizer increased the concentration of phosphate: 118% and 132% in the cultivation soil and plant cells, respectively. These show that BIOACTIVE may have potential as an effective biofertilizer in agriculture.

Effect of Microbe Control and Water Temperature on Early Growth and Yield of Soybean Sprouts (콩 종실의 미생물 제어방법과 수주온도에 따른 콩나물의 초기 생육 및 수율)

  • 배경근;남승우;김경남;황영현
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.6
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    • pp.453-458
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    • 2002
  • High temperature sterilization method (30min. at $40^{\circ}C$longrightarrow30 min. at $70^{\circ}C$longrightarrow30 min. cooling at $20^{\circ}C$) was evaluated better than that of chloride, ion water, $O_3$ water, and salt water for the controling of micro-organisms at the early stage of sprout culture. Due to the improved germination rate and effective control of micro-organisms at early stage, the method resulted in much lower in the number of micro-organisms, higher in sprout yield, and 2 days longer in quality keeping at market than sprouts prepared by conventional method. There are two methods related with water temperature; constant temperature method (18.5$\pm$ $0.5^{\circ}C$) which keeps the same water temperature during the culture and 3-stage temperature method which changes the water temperature depending on the days after culture. Three-stage temperature method set the temperature at 21$\pm$ $1^{\circ}C$) in the second and third days after the initial acceleration of germination (about 4 hours), at 18.5$\pm$0.5$^{\circ}C$ in the fourth and fifth days, and at 17.0$\pm$ $0.5^{\circ}C$) in the sixth and seventh days. Three-stage temperature method could enhance the resistance power to diseases at the early growth stage, control the growth to standard goods (8-9cm in length and 2.15-2.30mm in width), and keep good body color at the middle or final stage. This method also increased the sprout yield by 6% compared with the conventional method, constant temperature method.

The Effect of Chitosan and Wood Vinegar Treatment on the Growth of Eggplant and Leaf Lettuce (키토산과 목초액 처리가 가지 및 잎상추의 생육에 미치는 영향)

  • Jeong, Soon-Jae;Oh, Ju-Sung;Seok, Woon-Young;Cho, Mi-Yong;Seo, Jung-Bum
    • Korean Journal of Organic Agriculture
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    • v.15 no.4
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    • pp.437-452
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    • 2007
  • From the experiments on the effect of chitosan and wood vinegar, which are environment-friendly materials, on the growth of eggplant and leaf lettuce, the following results were obtained. 1. The effect of chitosan and wood vinegar treatment on the growth of eggplant 1) There was no difference in soil component elements before and after treatment with chitosan and wood vinegar. The total number of microorganisms tended to increase after treatment with chitosan and wood vinegar, while the number of bacteria increased among microorganisms. However, there was no consistent tendency in the number of Acinomycetes, fungi, and trichodema between treatments. 2) The growth and the yield of eggplant increased compared with the control plot in both the chitosan-treated plot and the wood vinegar-treated plot. However, there was no significant difference between the treatments. The yield of eggplant per 10a increased in chitosan- and wood vinegar-treated plots compared with the controlled plot. There was no significant difference in treatments, however, the plot treated with 800 times-diluted solution showed a higher growth. 2. The effect of chitosan and wood vinegar treatment on the growth of leaf lettuce 1) There was no difference in soil component elements before and after treatment with chitosan and wood vinegar. The number of bacteria increased among microorganisms. However, there was no consistent tendency in the number of Acinomycetes, fungi, and trichodema between treatments. 2) The growth of leaf lettuce in both chitosan-treated plot and wood vinegar-treated plot increased compared with the control plot, however, there was no significant difference between the treatments. On the whole, the plot treated with greater concentration showed a higher growth.

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Detection of Pediococci in Kimchi Using Pediococci Selective Medium (Pediococci 선택배지를 이용한 김치 유래 Pediococci 검출)

  • Lee, Myeong-Jae;Lee, Jong-Hoon
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.238-242
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    • 2009
  • Pediococci selective medium (PSM) supplemented with ampicillin (A) reported as valid for the detection and enumeration of pediococci included in foods and animal feed was evaluated for the selective detection of the genus Pediococcus in kimchi. PSM is based on the complex basal medium MRS supplemented with cysteine hydrochloride, vancomycin, novobiocin, and nystatin. In the medium evaluation with known species, the growth inhibition of leuconostocs, Pediococcus pentosaceus, Lactobacillus casei, Lactobacillus curvatus, Oenococcus oeni, and Streptococcus thermophilus was not confirmed. In the application of kimchi samples on the selective medium, leuconostocs, P. pentosaceus, Weissella koreensis, Lb. curvatus, Lactobacillus brevis, and Lactobacillus sakei were detected. PSM+A was proved to be not applicable for the detection of pediococci in kimchi.

Studies on the morphological and physiological characteristics of isolated strains from rotting ginseng (인삼부패곰팡이의 형태 및 생리학적 특성에 관한 연구)

  • 정동곤;박길동;하승수;주현주
    • Microbiology and Biotechnology Letters
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    • v.14 no.5
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    • pp.391-397
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    • 1986
  • Three kinds of microoganisms were isolated and identified from the ginseng and ginseng products to research the properties of the molds which spoil the ginseng and ginseng products. The results obtained were as follows: (1) The predominant strains on ginseng products were Aspergillus sp., Penicillium sp.-A and Penicillium sp.-B. These predominant fungi deteriorated ginseng products exclusively, (2) Aspergillus sp. showed the greatest mycelial growth at $40^{\circ}C$ and its optimum pH was 5, meanwhile Pencillium sp. showed the greatest mycelial growth at $30^{\circ}C$ and its optimum pH was 3. (3) The growth of the isolated strains was stimulated with the increase in the concentration of saponin at the lower concentration, meanwhile it was inhibited at 1.0% concentration of saponin.

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