• Title/Summary/Keyword: 미생물제제

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Studies on the Isolation and Identification of Bacillus sp. for the Composting of Swine Manure and the Removal of Malodorous Gases from its Liquid Compost (돈분의 퇴비화를 위한 Bacillus sp.의 분리 동정 및 그 액체 비료의 악취 제거 연구)

  • 김규동;김기연;함영태
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.154-159
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    • 2004
  • Bacillus species were isolated from swine manure to develope the microbial additive suitable for the rapid com-posting. The 3 of 4 isolated strains were identified as Bacillus cereus KD-2, B. pumilus KD-3, and B. licheni-formis KD-4. Bacillus sp. KD-1 was, however, not highly identical with any Bacillus sp. The isolated strains were analyzed their growth rates, enzyme activities, and antibacterial activities. The maximum growth tem-peratures of KD-1, KD-2, KD-3 and KD-4 were $45^{\circ}C$, $50^{\circ}C$, $53^{\circ}C$, and $55^{\circ}C$, respectively. The activities of pro-tease or amylase in mixed culture of 4 strains were similar in the range of $37^{\circ}C$ to $53^{\circ}C$ and activities of lipase in the range of $37^{\circ}C$ to $42^{\circ}C$ were twice higher than those of lipase in the range of $47^{\circ}C$ to $53^{\circ}C$. The antibacterial activity of KD-l, KD-2, or KD-3 against each other was not detected. That of KD-4 against KD-1, KD-2, or KD-3 was, however, detected. The organic compound and C/N ratio of compost fermented by the mixed culture were determined as 61.9% and 22.4%, respectively. The concentration of the ammonia gas was 12.35 mg/l in the liquid compost.

Biocontrol of Vegetables Damping-off by Bacillis ehimensis YJ-37 (Bacillus ehimensis YJ-37에 의한 채소류 모잘록병의 생물학적 방제)

  • 김진호;최용화;강상재;이인구;주길재
    • Journal of Life Science
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    • v.12 no.4
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    • pp.416-422
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    • 2002
  • Bacillus ehimensis YJ-37 was observed as a potential biological agent to control the occurrence of diseases and plant growth.promoting rhizobacteria (PGPR). Population density of B. ehimensis YJ-37 were higher 1.2~2 times in main roots and lateral roots than from nonrhizosphere soil and persisted around 10$^4$g root on the watermelon and radish root system upto 30 days after growing in pot condition. As a PGPR, B. ehimensis YJ-37 enhanced plant growth of watermelon and radish by soil treatment. The leaf area, hypocotyl length, root length and dry weight of radish were about 85, 33, 23 and 89% more than that of untreated plant, respectively. In case of watermelon were about 63, 27, 25 and 69% more than that of untreated plant, respectively. Biocontrol of damping-off in watermelon and radish caused by Rhizoctonia solani AG-4 and Pythium ultimum were carried out in pots using 3. ehimensis YJ-37. The results showed that might contribute to it's suppression of damping-off disease in field plants.

Efficacy of Mixture of Lactic Acid Bacteria (LAB) and Bifidobacteria Supplement in the Management of Constipation; Demonstration of Functionality in Animal and Clinical Trials (한국형 Bifidobacterium longum SPM1205 혼합제제의 변비 개선 효과)

  • Kim, Jung-Rae;Lee, Do-Kyung;Baek, Eun-Hye;An, Hyang-Mi;Yang, Hwan-Jin;Kim, Mi-Jin;Choi, Kyung-Soon;Yun, Mi-Eun;Jung, Yi-Jung;Oh, Pok-Ja;Chung, Myung-Jun;Ha, Nam-Joo
    • Korean Journal of Microbiology
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    • v.46 no.1
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    • pp.52-62
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    • 2010
  • The aim of this study was to evaluated the efficacy of mixture of Lactic Acid Bacteria (LAB) and bifidobacteria supplement, which are contained with Lactobacillus acidophilus, Bifidobacterium longum SPM1205, and Pediococcus pentosaceus for the management of constipation in animal model and clinical trials. 5 ICR mice and 4 female constipation subjects were orally taken mixture of LAB and bifidobacteria for 2 weeks. We investigated the number of fecal LAB and harmful enzymes activities before and after mixture of LAB and bifidobacteria application. As a result, fecal LAB count was increased and harmful enzymes activities of intestinal microflora were generally decreased after mixture of LAB and bifidobacteria application. Also, 61 female subjects were randomly assigned to receive either mixture of LAB and bifidobacteria or lactose and were taken three times a day for 2 weeks. Then, we analyzed mixture of LAB and bifidobacteria effect through the questionnaires. Daily consumption of this mixture of LAB and bifidobacteria improved the constipation in constipation group (56.3%) compared with lactose application group (26.7%). Furthermore, after mixture of LAB and bifidobacteria treatment, frequency of hard stool decreased from 0.22 to 0.03. These results indicated that mixture of LAB and bifidobacteria application is effective to improve the constipation.

Microbial Degradation of Fats and Oils in Industrial Wastewater (유지폐수의 생물학적 처리에 관한 연구)

  • 박춘호;김용기;오평수;유희종
    • Microbiology and Biotechnology Letters
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    • v.19 no.1
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    • pp.88-93
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    • 1991
  • The biodegradable bacteria for fats and oils were isolatcd from soil and wastewater. The isolated strain was designated as LW-27 which had high COD removal rate and biodegr2idability on fats and oils, and was identified as pseudomonas chlrorapihis. The cell viability of LW-27 which produced by vacuum drying at $45^{\circ}C$ for 24 hours was 82%. When the wastewater was mixed with LW-27 agent (0.1g/ day) on the activated sludge unit, the removal rates of COD, BOD and n-hexanc extract of the effluents were about 92.9%, 94.8% and 98.0%, respectively.

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Manufacturing Fermented Rapeseed Meal Compost using Two Microbial Agents and the Effect of Their Application (유용 미생물 제제 이용 발효 유채박 비료 제조 및 시용 효과)

  • Lee, Ji-Eun;Park, Won;Kim, Kwang-Soo;Lee, Yong-Hwa;Kwon, Da-Eun;Moon, Youn-Ho;Cha, Young-Lok;Kang, Yong-Ku
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.1
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    • pp.55-62
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    • 2019
  • Rapeseed meal, which is a byproduct of rapeseed oil extraction, improves crop productivity by supplying nutrients to the soil. The present study aimed to manufacture fermented rapeseed meal compost using two effective microbial agents and evaluate their efficiency as fertilizer. To types of fermented rapeseed meal, manufactured using either a bio-carrier or microbial agent, showed no differences in pH, electrical conductivity (EC), and total nitrogen content. However, the contents of $NH_4-N$ and $NO_3-N$ as inorganic nitrogen were increased by 5.6 times and 1.5 times, respectively, after 5 d of fermentation. Rapeseed meal fermented for 5 d was applied to tomato a basal fertilizer and after eight weeks, the plant height increased in all fermented rapeseed treatments compared to that in the chemical fertilizer treatment, and also the quantum yield of photosystem II (PS II) showed the same trend. The total nitrogen content of tomato leaves treated with a microbial fermented rapeseed meal was twice as high as that of that treated with a chemical fertilizer. It was confirmed that the increase in the tomato height was an effect of the rapeseed meal containing inorganic nitrogen, which can easily be absorbed by plants. From these results, it is considered that fermented rapeseed meal manufactured with an effective microbial agent for 5 d showed the highest inorganic nutrient content and greatest growth enhancement in tomato.