• Title/Summary/Keyword: 미나리과

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Analysis of Gene Diversity and Phenetic Relationship of Water Dropwort Species in Korea Using RAPD (OPB Primers) Markers (한국 미나리 집단에 대한 RAPD (OPB 프라이머)에 의한 유전적 다양성과 표현형 관계)

  • Huh, Man Kyu
    • Journal of Life Science
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    • v.32 no.8
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    • pp.595-600
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    • 2022
  • Water dropworts, Oenanthe javanica and O. javanica var. japonica are called "minari" in Korea and are eaten as a vegetable. Cicuta virosa is common European water hemlock and has toxic properties, such as cicutoxin. Molecular variations of water dropwort species in Korea were investigated using random amplified polymorphic DNA (RAPD). The six populations were studied with 10 primers (Operon, OPB) for RAPD analysis. The 72 DNA fragments (bands) were found among six populations. Among these 72 bands, 61 (84.7%) bands were polymorphic. The typical populations of Cicuta virosa in Korea were small, isolated, and patchily distributed for natural populations and they maintained a high level of genetic diversity. However, when cultivated populations of O. javanica var. japonica were large and widely grown in rice paddies as vegetables, they maintained a lower genetic diversity than those of C. virosa and wild populations of O. javanica. Although the diversity indices of wild populations were shown to be higher than those of cultivated populations, no significant difference for measures of genetic variability was shown. Total genetic diversity value (HT) was 0.342. The interlocus variation in the within-population genetic diversity (HS) was 0.201. The proportion of total genetic variation due to differences among populations (GST) range was 0.414, indicating that 41.4% of the total variation was among populations. In conclusion, the RAPD technique was a useful method for discrimination between C. virosa and O. javanica. In addition, RAPD-OPB markers could further distinguish the strains from different food sources.

Uptake of Heavy Metal Ions by Water Dropwort (Oenanthe stolonifera DC.) and Identification of Its Heavy Metal-Binding Protein (미나리의 중금속 흡수량 측정 및 중금속 결합단백질의 동정)

  • Park, Young-Il;Kim, Hee-Guen;Kim, Yoo-Young;Kim, In-Soo
    • Applied Biological Chemistry
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    • v.39 no.6
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    • pp.494-500
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    • 1996
  • Uptake of hen metal ions by water dropwort (Oenanthe stolonifera DC.) and its cadmium-binding protein were studied to probe for good method to remove heavy metal contaminants from environments. The plant was cultured in the culture medium (pH 7.0) containing the various concentrations of $Cd^{2+}$, $Cr^{3+}$ or $Pb^{2+}$, for 3 and 7 days. The residual heavy metals deposited in roots linearly increased as the metal ions concentration increased up to 17 ppm for $Cd^{2+}$, 20 ppm for $Cr^{3+}$ and 50 ppm for $Pb^{2+}$. Above these concentrations, the plant growth was inhibited and the uptake rates of the metal ions decreased. The heavy metals absorbed by the plant were mostly deposited in roots. In particular, the residual concentration of lead in roots was about four times higher than those of cadmium and chromium. When cultured in the medium containing 20 ppm of each metal ion, 80% of cadmium, 90% of cromium and 96% of lead were deposited in roots out of the total residual metal ions in the plant. These values correspond to 6.1 mg of cadmium, 5.2 mg of chromium and 23.6 mg of lead per one gram of roots tissue on a dry weight basis. A cadmium-binding protein was partially purified by extraction, gel filtration and DEAE-Cellulose chromatography from water dropworts that was grown in the medium containing 20 ppm $Cd^{2+}$. The purified protein was a single band on SDS- and non-denaturing- polyacrylamide gel electrophoresis. Its molecular mass was estimated to be ca. 5,000 dalton by gel filteration. Analysis of amino acid composition of the protein indicated that it had a typical amino acid composition of heavy metal-binding protein in that it contained 27% of acidic amino acids and 9.9% of cysteine. However, it is likely that the protein is a new plant metal-binding protein, since its amino acid composition is somewhat different from those of phytochelatins that have been known so far.

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Feasibility of Aquatic Plants (Eichhornia crassipes and Water dropwort) for Nutrients Removal (수생식물(부레옥잠 및 미나리)을 이용한 영양염류 제거에 관한 연구)

  • Choi, Don-Hyeok;Kang, Ho;Lee, Mi-Kyung
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.2
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    • pp.141-148
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    • 2010
  • Feasibility of floating aquatic plants (Eichhornia crassipes and Water dropwort) was investigated in order to control of sewage depending on various initial loading in a lab scale test. In addition, field test was conducted to assess the uptake rate of nutrient by E. crassipes. Lab-scale test applying primary domestic effluent operated at 4 day HRT shows that the highest uptake rates were 1.06 g N/$m^2{\cdot}day$ and 0.39 g P/$m^2{\cdot}day$ in the E. crassipes reactor. BOD removal efficiency in E. crassipes reactor was as high as 80% when the loading value was lower than 185 kg BOD/$ha{\cdot}day$. While 70 ~ 80% removal efficiency of BOD was achieved when the loading value was lower 80 kg BOD/$ha{\cdot}day$ at the W. Dropwort reactor. Experiment results show that E.crassipes has a higher nutrients removal efficiency than W. dropwort under high pollutant loading. Input loadings of TN and TP should not exceed to 10 kg TN/$ha{\cdot}day$ and 2.0 kg TP/$ha{\cdot}day$ respectively to provide a 50% TN and 80% TP removal efficiencies using E. crassipes. The field test demonstrated that an annual yield of E. crassipes mass was estimated as a fresh weight of 30.9 $m^3/ha{\cdot}yr$. E. crassipes grown in field pads absorbed 76.7 kg N/$ha{\cdot}yr$ and 13.4 kg P/$ha{\cdot}yr$ as a dry weight.

Inhibitory Effects of Lyophilized Dropwort Vinegar Powder on Adipocyte Differentiation and Inflammation (미나리 발효 식초의 지방세포 분화억제 및 항염증 효과)

  • Park, Yun-Hee;Choi, Jun-Hyeok;Whang, Key;Lee, Syng-Ook;Yang, Seun-Ah;Yu, Mi Hee
    • Journal of Life Science
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    • v.24 no.5
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    • pp.476-484
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    • 2014
  • Obesity, which is characterized by a state of mild chronic inflammation, is known to cause metabolic diseases. This study was carried out to investigate the effect of lyophilized dropwort vinegar powder (DVP) on adipocyte differentiation and inflammation in T3-L1 preadipocyte and RAW 264.7 macrophage cell lines. DVP inhibited the differentiation of 3T3-L1 preadipocytes induced by a mixture of IBMX, dexamethasone, and insulin (MDI). Western blot analysis of cell lysates showed that DVP decreased the levels of two major transcription factors involved in adipogenesis, peroxisome proliferator- activated receptor-${\gamma}$ (PPAR-${\gamma}$) and CCAAT-enhancer-binding protein ${\alpha}$ ($C/EBP{\alpha}$). DVP also significantly suppressed lipopolysaccharide (LPS)-induced production of nitric oxide (NO), and this was accompanied by a decrease in inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. These results demonstrate that DVP inhibits MDI-induced adipocyte differentiation of 3T3-L1 cells and LPS-induced inflammation in RAW 264.7 macrophage cells. The findings indicate that this natural product may be a good candidate as to prevent metabolic diseases.

Effects of Phytol and Small Water Dropwort Extract on the T Subset in the Sarcoma 180-Transplanted Mice (Phytol과 들미나리추출물이 Sarcoma 180마우스의 T Subset에 미치는 효과)

  • 김광혁;장명웅;박건영;이숙희;류태형;선우양일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.4
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    • pp.405-411
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    • 1993
  • Several studies have shown that phytol isolated from perilla leaf and small water dropwort (Oenanthe javanica (BL) D.C., wild type) extract reveal antirumor activities. In the present study we investigated the effect of phytol and the small water dropwort extract on the T subset in the sarcoma 180-transplanted mice in order to elucidate the immunological mechanism of antiturmor activity of these substances. The results obtained from the experiment were as follows : When phytol was injected into the sarcoma-180 transplanted mice (control), the levels of T cell and T subset by inoculation of the tumor cells were enhanced, but that of T cells in case of small water dropwort extract was similar to the control. Asialo GM1$^{+}$ cells were increased when phytol or small water dropwort extract with injected into tumor-transplanted mice. In normal mice the number of asialo GM1$^{+}$ cells increased with phytol injection and decreased with small water dropwort extract injection. L3T4$^{+}$/Lyt-2$^{+}$cell ratios were decreased when phytol was injected into tumor-transplanted mire, but increased in case of small water dropwort extract injection. In normal mice the ratios showed large decreases with phytol or small water dropwort extract injection. These results indicate that phytol or small water dropwort extract ran activate the proliferation of natural killer cells that are effector cells in tumor-bearing mice.

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Anti-Inflammatory Effects of Oenanthe javanica Ethanol Extract and Its Fraction on LPS-Induced Inflammation Response (Lipopolysaccharide로 유도한 대식세포의 염증반응에서 미나리 에탄올 추출물 및 분획물의 항염증 효과)

  • Jang, Ji-Hun;Cho, Hyun-Woo;Lee, Bo-Young;Yu, Kang-Yeol;Yoon, Ji-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.11
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    • pp.1595-1603
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    • 2016
  • The present study examined the anti-inflammatory effects of Oenanthe javanica ethanol extract (OJE) and its fraction on the lipopolysaccharide (LPS)-induced inflammatory response in RAW 264.7 macrophage cells. OJE remarkably reduced protein expression of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2), resulting in inhibition of production of nitric oxide (NO). In order to identify the anti-inflammatory effects of bioactive fractions, OJE was fractionated into hexane, dichloromethane, ethyl acetate, and n-butanol fractions. The results show that the ethyl acetate and dichloromethane fractions reduced production of NO without cytotoxicity. Especially, the ethyl acetate fraction effectively reduced protein expression of iNOS and COX-2. Proinflammatory cytokine production was also reduced by ethyl acetate fractions in LPS-induced RAW 264.7 cells. These data suggest that OJE and its fraction possess pharmacological activity and might be useful for development of anti-inflammatory agents or dietary supplements.

Recipes for dishes controlling diabetes and its blood glucose response effect (당뇨조절식의 조리법과 혈당반응효과)

  • 김명애;김연선;윤석권
    • Korean journal of food and cookery science
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    • v.15 no.4
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    • pp.401-409
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    • 1999
  • This stydy was performed to improve recipes for dishes with seaweed, spinach, wild parsley, and yam as food source of low blood glucose and to select better favorite dishes controlling diabetes trough sensory test of 9 scoring method. Also the effect controlling blood glucose based on observation of blood glucose and glycemic index was applied for the normal person. The traditional recipes gained high acceptability in the sensory test. The Segogiguk, Daenjanguk, Guonsaeujuk, Sujebi, Bokeum and Beoseokhoi of seaweed ranged from 8.2 to 7.2. The Danggun juice, Daenjangguk, jeon and Dubumuchim of spinach was evaluated as 8.2 to 7.3. The Gulsaengche, Beefmuchim, Juice and Chodae of wild parsley showed high acceptability as 7.9 to 6.8. The Jorim and Jeon of yam ranged form 7.7 and 6.8. Addording to the result of blood glucose response of normal person meals controlling dishes showed GI 57.9-GI 91.3 compared to GI 100 of usual meal. The rice boiled with 30% of black soybean and additional dishes controlling diabetes were most effective as GI 57.9.

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An Investigation and Evaluation of Insect Pests in Greenhouse Vegetables in Jeonbuk Province (전북지역 시설 채소류 작물별 해충 발생양상 및 종 동정)

  • Lim, Ju-Rak;Park, Sung-Hee;Moon, Hyung-Cheol;Kim, Ju;Choi, Dong-Chil;Hwang, Chang-Yeon;Lee, Kwan-Suk
    • Korean journal of applied entomology
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    • v.51 no.3
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    • pp.271-280
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    • 2012
  • Twenty-two families and 39 species of insect pests were surveyed on five families and 20 species of greenhouse vegetables in Jeonbuk province. The species of insect pests and the families of plants infested were seven families and 10 species on Chenophodiaceae, 16 families and 25 species on Brassicaceae, nine families and 10 species on Apiaceae, six families seven species on Liliaceae, and 13 families and 29 species on Compositae. Spodoptera exigua H$\ddot{u}$bner and Spodoptera litura Fabricius occurred on all vegetables. Additionally, Frankliniella intonsa Trybom, Trialeurodes vaporariorum Westwood, Myzus persicae Sulzer, and Phytomyza horticola Goureau occurred on all vegetables except Liliaceae(Allium tuberosum Rottl. and Allium fistulosum L.). Thirteen species of insect pests including Hymenia recurvalis Fabricius occurred only one vegetables, indicating that they were monophagous. The main insect pests of Chenophodiaceae were M. persicae, H. recurvalis, S. exigua and S. litura whereas Dolycoris baccarum Linn$\acute{e}$, Trialeurodes vaporariorum Westwood, Trichoplusia ni H$\ddot{u}$bner, and P. horticola were not recorded. On Brassicaceae were Brevicoyne brassicae Linn$\acute{e}$, M. persicae, Phaedon brassicae Baly, Phyllotreta striolata Fabricius, Plutella xylostella Linn$\acute{e}$, Hellula undalis Fabricius, S. litura, Pieris rapae Linn$\acute{e}$, Artogeia rapae Linn$\acute{e}$, and Athalia rosae ruficornis Jakovlev, but six species including Frankliniella intonsa Trybom were not recorded. The degree of damage by insect pests on Apiaceae was low, five species including Tetranychus kanzawai kishida, F. intonsa, T. vaporariorum, S. litura, and P. horticola were not recorded. The main insect pests on Liliaceae were Thyatira tabaci Lindeman, Acrolepiopsis sapporensis Matsumura, S. exigua, and Liriomyza chinensis Kato. The damage to Compositae by insect pests was relatively low except that of S. litura.

Studies on the Umbelliferous Fruits (XI) -'Kobon' (Korean Gaoben)- (미나리과(科) 식물(植物)의 과실(果實)에 관한 연구(硏究)(XI) -고본에 대하여-)

  • Suk, Kuy-Duk
    • Korean Journal of Pharmacognosy
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    • v.6 no.3
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    • pp.131-135
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    • 1975
  • In continuation of studies on the Umbelliferous fruits the inner morphology of 'Kobon' fruit was examined, 'Kobon' is an original plant of Korean Gaoben, its scientific name being given Angelica tenuissima NAKAI or Ligusticum tenuissimum KITAGAWA. The arrangement of vascular bundles is the same as those of the genus Ligusticum or Tilingia and Conioselinum. The tracheids reach nearly the top of the ribs, and this is similar to that of the genus Conioselinum. From these characters and the results of studies on the inner morphology of the fruits of Umbelliferous plants related to 'Kobon,' the origin of 'Kobon' was identified as the genus Conioselinum.

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Antimutagenic Effect of Flavonoids Isolated from Oenanthe jauanica (미나리에서 분리한 플라보노이드 화합물의 아플라톡신 B$_1$에 대한 항돌연변이 효과)

  • 박종철;하정옥;박건영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.4
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    • pp.588-592
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    • 1996
  • The flavonoids isolated from Oenanthe javanica were investigated on the antimutagenic effect againt aflatoxin $B_1(AEB_1).$ The 0.5 and 1.0mg/plate of isorhamnetin reduced the mutagenicity of $AFB_1about$ 33 and 59%, respectively. Persicarin showed the 40 and 47% inhibition rate at Previous concentration, respectively.

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