Browse > Article
http://dx.doi.org/10.5352/JLS.2014.24.5.476

Inhibitory Effects of Lyophilized Dropwort Vinegar Powder on Adipocyte Differentiation and Inflammation  

Park, Yun-Hee (Department of Food Science and Technology, Keimyung University)
Choi, Jun-Hyeok (Department of Food Science and Technology, Keimyung University)
Whang, Key (Department of Food Science and Technology, Keimyung University)
Lee, Syng-Ook (Department of Food Science and Technology, Keimyung University)
Yang, Seun-Ah (The Center for Traditional Microorganism Resources, Keimyung University)
Yu, Mi Hee (Department of Food Science and Technology, Keimyung University)
Publication Information
Journal of Life Science / v.24, no.5, 2014 , pp. 476-484 More about this Journal
Abstract
Obesity, which is characterized by a state of mild chronic inflammation, is known to cause metabolic diseases. This study was carried out to investigate the effect of lyophilized dropwort vinegar powder (DVP) on adipocyte differentiation and inflammation in T3-L1 preadipocyte and RAW 264.7 macrophage cell lines. DVP inhibited the differentiation of 3T3-L1 preadipocytes induced by a mixture of IBMX, dexamethasone, and insulin (MDI). Western blot analysis of cell lysates showed that DVP decreased the levels of two major transcription factors involved in adipogenesis, peroxisome proliferator- activated receptor-${\gamma}$ (PPAR-${\gamma}$) and CCAAT-enhancer-binding protein ${\alpha}$ ($C/EBP{\alpha}$). DVP also significantly suppressed lipopolysaccharide (LPS)-induced production of nitric oxide (NO), and this was accompanied by a decrease in inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. These results demonstrate that DVP inhibits MDI-induced adipocyte differentiation of 3T3-L1 cells and LPS-induced inflammation in RAW 264.7 macrophage cells. The findings indicate that this natural product may be a good candidate as to prevent metabolic diseases.
Keywords
3T3-L1 adipocyte; anti-inflammatory; anti-obesity; dropwort vinegar; RAW 264.7 macrophages;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Lee, K. L., Park, K. Y. and Rhee, S. H. 1992. Antimutagenic effect of green-yellow vegetables toward aflatoxin B1 and 4-nitroquinoline-1-oxide. J Korean Soc Food Nutr 21, 143-148.
2 McNeel, R. L. and Mersmann, H. J. 2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and differentiation. J Nutr Biochem 5, 266-274.
3 Costante, C. and Harry, E. 1971. pH as a Determinant of cellular growth and contact inhibition. Proc Natl Acad Sci USA 68, 229-233.   DOI   ScienceOn
4 Attie, A. D. and Scherer, P. E. 2009. Adipocyte metabolism and obesity. J Lipid Res 50, 395-399.   DOI
5 Bradford, B. L. and Bruce, M. S. 2000. Towards a molecular understanding of adaptive thermogenesis. Nature 404, 652-660.   DOI
6 Cornelius, P., MacDougald, O. A. and Lane, M. D. 1994. Regulation of adipocyte development. Annu Rev Nutr 14, 99-129.   DOI   ScienceOn
7 Darington, G. J., Ross, S. E. and MacDougald, O. A. 1998. The role of C/EBP genes in adipocyte differentiation. J Biol Chem 273, 30057-30060.   DOI
8 Fujita, T., Kadoya, Y., Aota, H. and Nakayama, M. 1995. A new phenylpropaniod glucoside and other constituents of Oenanthe javanica. Biosci Biotechnol Biochem 59, 526-528.   DOI   ScienceOn
9 Funk, C. D. 2001. Prostaglandins and leukotrienes; advances in eicosanoid biology. Science 294, 1871-1875.   DOI   ScienceOn
10 Gokhan, S. H. 2006. Inflammation and metabolic disorders. Nature 444, 860-867.   DOI   ScienceOn
11 Green, L. M., Reade, J. L. and Ware, C. F. 1984. Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods 70, 257-268.   DOI   ScienceOn
12 Gregor, M. F. and Hotamisligil, G. S. 2011. Inflammatory mechanisms in obesity. Annu Rev Immunol 29, 415-445.   DOI   ScienceOn
13 Hauser, S., Adelmant, G., sarraf, P., Wright, H. M., Mueller, E. and Spiegelman, B. M. 2000. Degradation of the peroxisome proliferator-activated receptor gamma is linked to ligand-dependent activation. J Biol Chem 275, 18527-18533.   DOI   ScienceOn
14 Suganami, T., Nishida, J. and Ogawa, Y. 2005. A paracrine loop between adipocytes and macrophages aggravates inflammatory changes: role of free fatty acids and tumor necrosis factor ${\alpha}$. Arterioscler Thromb Vasc Biol 25, 2062-2068.   DOI   ScienceOn
15 Tamashita, H., Fujisawa, K., Lto, E., Idei, S., Kawaguci, N., Kimoto, M., Hiemori, M. and Tsuji, H. 2007. Improvement of obesity and glucose tolerance by acetate in type 2 diabetic otsuka long-evans tokushima Fatty (OLETF) rats. Biosci Biotechnol Biochem 71, 1236-1243.   DOI   ScienceOn
16 Titheradge, M. A. 1998. The enzymatic measurement of nitrate and nitrite. Methods Mol Biol 100, 83-91.
17 Park, S. J., Choi, J. H., Jung, Y. S. and YU, M. H. 2013. Inhibitory effect of Rumex Crispus L. fraction on adipocyte differentiation in 3T3-L1 cells. Korean J Food Sci Technol 45, 90-96.   과학기술학회마을   DOI   ScienceOn
18 Visscher, T. L. S. and Seidell, J. C. 2001. The public health impact of obesity. Annu Rev Public Health 22, 355-375.   DOI   ScienceOn
19 Willeaume, V., Kruys, V., Mijatovic, T. and Huez, G. 1996. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharide in macrophages: similarities and differences. J Inflamm 46, 1-12.
20 Yun, H. Y., Dawson, V. L. and Dawson, T. M. 1996. Neurobiology of nitric oxide. Crit Rev Neurobiol 10, 291-316.   DOI   ScienceOn
21 Needleman, P., Turk, J., Jakschik, B. A., Morrison, A. R., Lefkowith, J. B. 1996. Arachidonic acid metabolism. Annu Rev Biochem; 55: 69-102, Herschman, H. R. Prostaglandin synthase 2. Biochim Biophys Acta 1299: 125-40.
22 Ohshima, T., Koga, H. and Shimotohno, K. 2004. Transcriptio-nal activity of peroxisome proliferator-activated receptor gamma is modulated by SUMO-1 modification. J Biol Chem 279, 29551-29557.   DOI   ScienceOn
23 Park, J. C., Yu, Y. B. and Lee, J. H. 1993. Isolation of steroids and flavonoids from the herb of Oenanthe javanica D.C. Korean J Pharmacogn 24, 244-246.   과학기술학회마을
24 Park, S. J., Lee, K. S. and An, H. L. 2007. Effects of dropwort powder on the quality of castella. J East Asian Soc Diet Life 17, 834-839.   과학기술학회마을
25 Rhee, H. J., Koh, M. S. and Choi, O. J. 1995. A study on the volatile constituents of the water dropwort (Oenanthe Stolonifera DC). Korean J Food Sci 11, 386-395.   과학기술학회마을
26 Hotamisligil, G. S., Shargill, N. S. and Spiegelman, B. M. 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259, 87-91.   DOI   ScienceOn
27 Shew, R. L., Papka, R. E., Mcneill, D. L. and Yee, J. A. 1993. NADPH-diaphorase-positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction. Peptides 14, 637-641.   DOI   ScienceOn
28 Si, Y. X., Wang, Z. J., Park, D., Jeong, H. O., Ye, S., Chung, H. Y., Yang, J. M., Yin, S. J. and Qian, G. Y. 2012. Effects of isorhamnetin on tyrosinase: inhibition kinetics and computational simulation. Biosci Biotechnol Biochem 76, 1091-1097.
29 Koutnikova, H. and Auwerx, J. 2001. Regulation of adipocyte differentiation. Ann Med 33, 556-561.   DOI   ScienceOn
30 Kim, J. B. and Park, J. Y. 2002. Molecular insights into fat cell differentiation and functional r oles of adipocytokine. J Korean Soc Endo 17, 1-9.
31 Kim, M. J., Lee, S. B., Choi, J. H., Kwon, S. H., Kim, H. D., Bang, M. H. and Yang, S. A. 2013. Characteristics of fermented dropwort extract and vinegar using fermented dropwort extract and its protective effects on oxidative damage in rat glioma C6 cells. Korean J Food Sci Technol 45, 350-355.   과학기술학회마을   DOI   ScienceOn
32 Kwon, S. H., Jeong, E. J., Lee, G. D. and Jeong, Y. J. 2000. Preparation method of fruit vinegars by two stage fermentation and beverage including vinegar. Food Ind Nutr 5, 18-24.
33 Kawamata, H., Ochiai, H., Mantani, N. and Terasawa, K. 2000. Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW 264.7 cells, a murine macrophage cell line. Am J Chin Med 28, 217-226.   DOI   ScienceOn
34 Herschman, H. R. Prostaglandin synthase 2. 1996. Biochem Biophys Acta 1299, 125-140.   DOI   ScienceOn
35 Wasserman, F. 1965. The development of adipose tissue. In Handbook of physiology, pp. 87-100, Renold, A., Cahill, G.F. eds., American Physiological Society: Washington, D. C., USA.
36 Rosen, E. D., Hsu, C. H., Wang, X., Sakai, X., Freeman, M. W., Gonzalez, F. J. and Spiegelman, B. M. 2001. $C/EBP{\alpha}$ induces adipogenesis through PPAR: a unified pathway. Genes Dev 16, 22-26.
37 Nanba, T. and Kato, H. 1985. Applications of mirin and non-salt miso to vinegar brewing. Nippon shokuhin Kogyo Gakkaishi 32, 731-737.   DOI