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Extraction of Nature Pigment with Antioxidant Properties from Sprout Barley - Optimization Using CCD-RSM (새싹보리로부터 항산화기능성을 갖는 천연색소의 추출 - CCD-RSM을 이용한 최적화)

  • Dong Hwan Kim;Seung Bum Lee
    • Applied Chemistry for Engineering
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    • v.35 no.3
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    • pp.222-229
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    • 2024
  • The use of low-toxic, hypoallergenic, and environmentally friendly natural pigments has increased. With growing interest in health, research on natural extracts containing beneficial substances for the human body is actively underway. In this study, natural pigments were extracted from sprout barley using a solvent extraction method and CCD-RSM was used to optimize the extraction process. The experiment's independent variables included extraction temperature, alcohol/ultra-pure volume ratio, and extraction time. The response variables were set to achieve a target chromaticity (L = 45, a = -35, b = 45), and to maximize DPPH radical scavenging activity evaluating the antioxidant capacity. The statistical significance of the main effect, interaction effect, and effect on the response value was evaluated and analyzed through the F and P values for the regression equation variables calculated using RSM optimization. Additionally, the reliability of the experiment was also confirmed through the P values of the probability plot graph. The extraction conditions for optimizing the four reaction values are 76.1 vol.% alcohol/ultra pure water volume ratio, an extraction temperature of 52.9 ℃ , and an extraction time of 49.6 min. Under these conditions, the theoretical values of the reaction values are L = 45.4, a = -36.8, and b = 45.0 DPPH radical scavenging activity = 30.9%. When the actual experiment was conducted under these optimal extraction conditions and analyzed, the measured values were L = 46.2, a = -36.1, and b = 48.2, and antioxidant capacity = 31.1% with an average error rate of 2.9%.

Analysis of Dietary Behavior and Related Factors According to the Level of Generalized Anxiety Disorder in Adolescents: Using the 16th-18th(2020-2022) Korea Youth Risk Behavior Survey (청소년의 범불안장애 수준에 따른 식생활 행태 및 관련 요인 분석: 제16차-제18차 (2020년-2022년) 청소년건강행태조사를 이용하여)

  • Kye, Eun Seul;Kim, Yookyung;Lee, Jung Woo
    • Journal of Korean Home Economics Education Association
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    • v.35 no.4
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    • pp.33-45
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    • 2023
  • The purpose of this study is to investigate the association between dietary behavior and related factors according to the generalized anxiety disorder (GAD) in adolescents. The results of analyzing demographic characteristics according to the GAD showed that gender, age, BMI, type of school, residence type, father's nationality, smoking and drinking experience, perceived health status, perception of body shape, economic status, academic achievement, physical activity, sedentary time and smartphone usage time were related to the GAD (p<0.01). The results of analyzing dietary behavior according to the GAD showed that the frequency of water intake, sweetened beverage intake, fast food intake, fruit intake and breakfast intake were related to the GAD (p<0.001). The results of analyzing depression and suicidality according to the GAD showed that depression, suicidal ideation, planning, and attempts were all related to the GAD (p<0.001). Even after adjusting for factors corresponding to demographic characteristics, it was confirmed that the level of GAD had a significant effect on depression and suicidality. This study is significant in that it provided basic data on adolescent mental health problems by analyzing dietary behavior and related factors according to the level of GAD.

A Study of Hunting Method of the Paleolithic People - Herd Hunting (구석기시대 사람들의 사냥방법 - 무리 사냥에 대한 고찰)

  • Taesop Cho
    • The Korean Journal of Quaternary Research
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    • v.31 no.2
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    • pp.23-30
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    • 2017
  • There are many uncertainty to understand the ways in which Paleolithic men hunted animals. But in the meantime, We can identify a part of the hunting activities of the old people through artworks left by the paleolithic people at that time. First is the size of the hunt. In the case of painted artwork, the size of the herd does not exceed 10 people. Therefore, a small group of about 10 people would go out for a hunt, and they could see that the size of the herd varied according to the hunting target. In other words, to catch large and wild beasts like bison, you can see that it consists of nine or seven groups. In order to catch the horse, the same large animal, we can see that six people joined together. On the other hand, a group of two or three people hunting a relatively small, mild animal such as a deer would have been enough. Therefore, the size of the hunting herd was less than 10 people, and the size of the hunting herd seemed to be slightly different for each animal. Second, it is confirmed that these groups have a well-divided role. Because all nine of them were hunted out, they were divided into four small groups, which is likely to imply that the role of each group was different. Third, we can see that most of the weapons of the hunting herds were spears. The production and use of stone tools have been progressively developed, making a small stoner, a small stoneware, and connecting it to the spears, making it more efficient to hunt. This kind of composite wool connecting stone tools and wood would have been the most efficient means of hunting.

A Discourse Analysis of Science Teachers' Scientific Modeling Activities: A Case from Earth Science Teacher Training (과학 모델링 활동에 나타난 교사의 담화 분석 -지구과학 교사 연수 사례-)

  • Heungjin Eom;Hyunjin Shim
    • Journal of The Korean Association For Science Education
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    • v.44 no.4
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    • pp.301-311
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    • 2024
  • We developed a small-group training program for in-service teachers focused on scientific modeling. We collected the discourses of the teachers who participated in the activity and analyzed them by type. The training program employed a collaborative approach in which a small group completed tasks and produced outputs based on the theme of 'galaxies and the Universe' to enable practical application in classes. Three in-service science teachers participated in the training program. Their discourses were recorded, transcribed, and classified into types based on individual turns and interaction units. The language expressions of the teachers reflected the unique characteristics of the teaching profession, with each participant having preferred language expression types, albeit with a generally low prevalence of specific language expression types across the participants. Differences in discourse characteristics related to the modeling theme, task presentation method, and model types, revealed that variations in the proportion of interaction unit types during the modeling design, build, and evaluation stages were primarily influenced by the teachers' familiarity with the modeling theme. While the task presentation method also influenced interaction types, model types had little impact on the distribution of interaction types. Considering these findings, training programs on modeling for in-service teachers should include a checklist to encourage sufficient interaction between participants as well as propose proper questions that can be effectively addressed through collaboration.

A Genotoxicity Study of Transgenic Tomatoes using CRISPR/Cas9 (CRISPR/Cas9에 의한 유전자교정 토마토의 유전독성평가)

  • Dong-Min Kang;Woo-Jin Jeong;Bashu Dev Neupane;Yu Jin Jung;Jong Mi Kim;Kwon Kyoo Kang;Mi-Jeong Ahn
    • Journal of Food Hygiene and Safety
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    • v.39 no.4
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    • pp.312-321
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    • 2024
  • Tomato is a widely distributed, cultivated, and commercialized vegetable crop. Recently, an increasing trend has been observed in the consumption of transgenic crops with enhanced functional components. However, consumer concerns regarding genotoxicity have been increasing. This study examined the genotoxicity of transgenic tomato (LTT) using the CRISPR/Cas9 system through a bacterial reverse mutation assay, chromosomal aberration assay, and mammalian micronucleus test. In the bacterial reverse mutation assay, LTT did not induce mutagenicity in Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537, or Escherichia coli WP2uvrA, irrespective of the presence or absence of S9. LTT did not cause clastogenic or aneugenic chromosomal abnormalities during metaphase in CHL cells. Moreover, LTT did not increase the frequency of micronucleated polychromatic erythrocytes in the polychromatic erythrocytes. These findings can be used as a foundation to assess the genotoxicity of transgenic crops using the CRISPR/Cas9 system in the future.

Esthetic restoration of maxillary anterior teeth considering facial features in digital diagnostic wax-up: a case report (디지털 진단 왁스업을 통하여 안모를 고려한 상악 전치부 심미수복 증례)

  • Sung-Ji Gong;Sang-Won Park;Hyun-Pil Lim;Kwi-dug Yun;Chan Park;Woohyung Jang
    • Journal of Dental Rehabilitation and Applied Science
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    • v.40 no.3
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    • pp.179-188
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    • 2024
  • To enhance the predictability of aesthetic treatment outcomes in aesthetic prosthetic restorations, considerations must include analysis of facial features, the relationship between teeth and lips, proportions of tooth width/length, gingival form, and more. Traditional diagnostic wax-ups have limitations in considering the patient's facial features and are unable to facilitate rapid form modifications. With recent advancements in digital technology, it is now possible to digitize the patient's facial features in three dimensions, enabling the design of restorations that harmonize with facial features. These digital workflows not only improve efficiency but also provide patients with faster visualization of treatment outcomes, thereby enhancing motivation. Therefore, in this case, a treatment plan is devised to utilize digital diagnostic wax-ups considering the patient's facial features for the final prosthetic design.

문헌검색(文獻檢索)에 있어서 Chemical Abstracts와 CA Condensates의 비교(比較)

  • Robert, B.E.
    • Journal of Information Management
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    • v.9 no.1
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    • pp.21-25
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    • 1976
  • 1975년(年) 3월(月), 4년반(年半) 동안의 Chemical Abstracts 색인(索引)과 온-라인이 가능(可能)한 CA Condensates를 비교(比較)하였다. 두가지 데이터 베이스를 함께 이용(利用)하여 검색(檢索)하는 방법(方法)이 가장 효율적(效率的)이지만 실예(實例)에서 보는 바와 같이 CA Condensates를 검색(檢索)하는 것이 보다 실용적(實用的)이다. System Development Corp 사(社) (SDC)에 설치(設置)되어 있는 온-라인 형태(形態)인 CHEMCON과 CHEM7071을 Chemical Abstracts 색인(索引)과 비교(比較)하였다. 대부분(大部分)의 Chemical Abstracts 이용자(理容者)들은 Chemical Abstracts 책자나 우가색인(累加索引)에는 친숙(親熟)하지만 CA Condensates는 아마도 그리 친숙(親熟)하지 못할 것이다. CA Condensates는 서지적 사항을 기계(機械)로 읽을 수 있는 형태(形態)로 되어 있고 Chemical Abstracts에 따라서 색인(索引)되므로 매주 발행되는 Chemical Abstracts 책자의 뒷 부분이 있는 색인(索引)과 같이 우리에게 가장 친숙(親熟)한 형태(形態)로 되어 있다. Chemical Abstracts가 현재(現在) 사용(使用)하고 있는 데이터 데이스이지만 본고(本稿)에서는 Index와 Condensates를 둘 다 데이터 베이스로 정의(定義)한다. Condensates가 미국(美國)의 Chemical Abstracts Service 기관으로부터 상업적(商業的)으로 이용(利用)할 수 있게 되자 여러 정보(情報)센터에서는 이용자(利用者)들의 프로 파일을 뱃취방식(方式)으로 처리(處理)하여 매주 나오는 자기(磁氣)테이프에서 최신정보(最新情報)를 검색(檢索)하여 제공(提供)하는 서어비스 (SDI)를 시작하였다. 어떤 정보(情報)센터들은 지나간 자기(磁氣)테이프들을 모아서 역시 뱃취방식(方式)으로 소급(遡及) 문헌검색(文獻檢索) 서어비스를 한다. 자기(磁氣)테이프를 직접 취급(取扱)하는 사람들을 제외(除外)하고는 대부분(大部分) Condensates를 아직 잘 모르고 있다. 소급(遡及) 문헌검색(文獻檢索)은 비용이 다소 비싸고 두서없이 이것 저것 문헌(文獻)을 검색(檢索)하는 방법(方法)은 실용적(實用的)이 못된다. 매주 나오는 색인(索引)에 대해서 두 개나 그 이상의 개념(槪念)이나 물질(物質)을 조합(組合)하여 검색(檢索)하는 방법(方法)은 어렵고 실용적(實用的)이 못된다. 오히려 주어진 용어(用語) 아래에 있는 모든 인용어(引用語)들을 보고 초록(抄錄)과의 관련성(關連性)을 결정(決定)하는 것이 때때로 더 쉽다. 상호(相互) 작용(作用)하는 온-라인 검색(檢索)을 위한 Condensates의 유용성(有用性)은 많은 변화를 가져 왔다. 필요(必要)한 문헌(文獻)만을 검색(檢索)해 보는 것이 이제 가능(可能)하고 어떤 항목(項目)에 대해서도 완전(完全)히 색인(索引)할 수 있게 되었다. 뱃취 시스팀으로는 검색(檢索)을 시작해서 그 결과(結果)를 받아 볼 때 까지 수시간(數時間)에서 며칠까지 걸리는 번거로운 시간차(時間差)를 이제는 보통 단 몇 분으로 줄일 수 있다. 그리고 뱃취 시스팀과는 달리 부정확하거나 불충분한 검색방법(檢索方法)은 즉시 고칠 수가 있다. 연속적인 뱃취 형태의 검색방법(檢索方法)에 비해서 순서(順序)없이 온-라인으로 검색(檢索)하는 방법(方法)이 분명(分明)하고 정확(正確)한 장점(長點)이 있다. CA Condensates를 자주 이용(移用)하게 되자 그의 진정한 가치(價値)에, 대해 논의(論義)가 있었다. CA Condensates의 색인방법(索引方法)은 CA Abstract 책자나 우가색인(累加索引)의 방법(方法)보다 확실히 덜 체계적(體系的)이고 철저(徹底)하지 못하다. 더우기 두 데이터 베이스는 중복(重複)것이 많으므로, 중복(重複)해서 검색(檢索)할 가치(價値)가 없는지를 결정(決定)해야 한다. 다른 몇 개의 데이터 베이스와 CA Condensates를 비교(比較)한 논문(論文)들이 여러 번 발표(發表)되어 왔는데 일반적(一般的)으로 CA Condensates는 하위(下位)의 데이터 베이스로 나타났다. Buckley는 Chemical Abstracts의 색인(索引)이 CA Condensates 보다 더 좋은 문헌 (데라마이신의 제법에 관해서)을 제공(提供)한 실례(實例)를 인용(引用)하였다. 죠오지대학(大學)의 Search Center는 CA Condensates가 CA Integrated Subject File 보다 기능(機能)이 못하다는 것을 알았다. CA Condensates의 다른 여러 가지 형태(形態)들을 또한 비교(比較)하였다. Michaels은 CA Condensates를 온-라인으로 검색(檢索)한 것과 매주 나오는 Chemical Abstracts 책자의 색인(索引)은 수작업(手作業)으로 검색(檢索)한 것을 비교(比較)한 논문(論文)을 발표(發表)하였다. 그리고 Prewitt는 온-라인으로 축적(蓄積)한 두 개의 상업용(商業用) CA Condensates를 비교(比較)하였다. Amoco Research Center에서도 CA Condensates와 Chemical Abstracts 색인(索引)의 검색결과(檢索結果)를 비교(比較)하고 CA Condensates의 장점(長點)과 색인(索引)의 장점(長點), 그리고 사실상(事實上) 서로 동등(同等)하다는 실례(實例)를 발견(發見)하였다. 1975년(年) 3월(月), 적어도 4년분(年分)의 CA Condensates와 색인(索引)(Vols 72-79, 1970-1973)을 비교(比較)하였다. 저자(著者)와 일반(一般) 주제(主題) 대한 검색(檢索)은 Vol 80 (Jan-June, 1974)을 사용(使用)하여 비교(比較)하였다. CA Condensates는 보통 세분화(細分化)된 복합물(複合物)을 검색(檢索)하는 데 불편(不便)하다. Buckly가 제시(提示)한 실례(實例)가 그 대표적(代表的)인 예(例)이다. 그러나, 다른 형태(形態)의 검색실예(檢索實例)(단체저자(團?著者), 특허수탁저(特許受託著), 개인저자(個人著者), 일반적(一般的)인/세분화(細分化)된 화합물(化合物) 그리고 반응종류(反應種類)로 실제적(實際的)인 검색(檢索)을 위한 CA Condensates의 이점(利點)을 예시(例示)하였다. 다음 실례(實例)에서 CHEMCON과 CHEM7071은 CA Condensates를 온-라인으로 입력(入力)시킨 것이다.

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Evaluation of Physiological Changes in Watermelon Stalk during Storage under Various Conditions of Treatments after Harvesting (수확 후 저장환경에 따른 수박줄기의 생리변화)

  • Park, Shin;Kang, Sun-Chul
    • Korean Journal of Environmental Agriculture
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    • v.24 no.1
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    • pp.56-60
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    • 2005
  • In order to increase the storage stability of watermelon stalk, storage environments such as temperature and other treatments such as vaseline, mixture of soybean oil and wheat flour, and coated paper label were tested separately after harvesting. At different storage temperatures (7, 18 and $30^{\circ}C$) shelf-life of stalk was inversely proportional to temperature. The results showed that during storage at $30^{\circ}C$ they were completely wilted in 7 days, at $18^{\circ}C$ in 15 days and at $7^{\circ}C$ in 19 days. Our data also showed that stability of watermelon stalk depending on temperature was very closely correlated with water content of watermelon stalk; during storage at $30^{\circ}C$ the water content of watermelon stalk was decreased to 21.1 % in 7 days, whereas at $7^{\circ}C$ the water content was decreased gradually to 71.2 % for 19 days of storage. In order to reduce the physiological changes in watermelon stalk at $30^{\circ}C$, following treatments such as vaseline, mixture of soybean oil and wheat flour, and coated paper label were tested. As a result, watermelon stalk without any treatment was completely wilted in 9 days, while stalks treated with vaseline and mixture of soybean oil with wheat flour were wilted in 15 and 12 days, respectively. The wilt delay was noted in the watermelon stalk for $3{\sim}6$ days during both treatments but the outward quality was found detracted, whereas when treated with coated paper label, the wilt of stalk was delayed for 3 days along with the improvement in the outward quality. Therefore this data indicate that the treatment of coated paper label during storage at $30^{\circ}C$ can be considered as a potent method for maintaining the physiology of watermelon stalk.

Studies on the Preparation and Utilization of Filefish Protein Concentrate (FPC) -I. The Preparation and Properties- (말쥐치 농축단백질(濃縮蛋白質)의 제조(製造) 및 이용(利用)에 관(關)한 연구(硏究) -제 1 보 : 제조조건(製造條件) 및 성질(性質)-)

  • Yang, Han-Chul;Son, Heung-Soo;Lim, Seung-Taik
    • Korean Journal of Food Science and Technology
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    • v.15 no.2
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    • pp.141-149
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    • 1983
  • The purpose of the present work is to find out the optimal conditions for the production of filefish protein preparations and to define the functional properties of the protein products. Fish protein concentrate (FPC) and fish protein isolate (FPI) were prepared by extraction of whole or headed and gutted filefish with various organic solvents. The results of the present study are as follows; 1. Among the solvents tested iso-propyl alcohol appeared to be the most effective for the extraction of lipid and also for that trimethylamine from the fish muscle. 2. The optimal extraction time showed to be 20 minutes with ethyl iso-propyl alcohol at $65-70^{\circ}C$under adequate mixing. 3. The most effective solvent ratio to the weight of fish material was proved to be 5:1 at the first extraction and to be 2:1 at the second stage. 4. The lipid content of the protein preparations reduced to below 0.5% by the third stage of extraction of headed or gutted filefish. The protein concentrate from whole fish, however, showed the lipid content of 0.27-0.31% only after the fifth stage of extration. 5. The protein contents of the protein concentrate and the protein isolate from whole filefish were 81.08% and 87.41% and the lipid contents of the two protein preparations were 0.43% and 0.45% respectively. 6. Higher calcium content was found in the protein concentrate rather than in the protein isolate. No sodium and potassium in the protein isolate were detected while the fish concentrate appeared to contain a considerable amounts of both elements. 7. The functional properties, such as suspended solids, wetability, emulsion stability and foam viscosity of the filefish protein isolates were proved to be higher than those of the protein concentrate.

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Thermal and Electrochemical Stability of Morpholinium Ionic Liquids (모폴린계 이온성 액체의 열 및 전기화학적 안정성)

  • Kim, Hyun-Taek;Hong, Yeon Ki;Kang, Jeong Won;Lee, Young-Woo;Kim, Ki-Sub
    • Korean Chemical Engineering Research
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    • v.50 no.4
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    • pp.702-707
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    • 2012
  • During the last few decades, toxic chemicals used in various industries have caused global pollution and the side products such as carbon dioxide and methane gas have contributed to global warming. Thus, it is desirable to develop new alternative solvents. It is well known that ionic liquids display a variety of environmentally friendly physical properties: nonvolatile, nonflammable, wide electrochemical windows, high inherent conductivities, wide thermal operating ranges, chemically inert, and limited miscibilities with organic solvents. Because of these characteristics, ionic liquids are promising candidates as solvents for synthetic chemistries, catalysis, and gas separations. In this study, we synthesized morpholiunium salts as N-ethyl-N-methylmorpholine Bromide, N-butyl-N-methylmorpholine Bromide, N-octyl-N-methylmorpholine Bromide, N-ethyl-N-methylmorpholine Tetrafluoroborate, N-butyl-N-methylmorpholine Tetrafluoroborate, N-octyl-N-methylmorpholine Tetrafluoroborate, N-ethyl-N-methylmorpholine Hexafluorophosphate, N-butyl-N-methylmorpholine Hexafluorophosphate, and N-octyl-N-methylmorpholine Hexafluorophosphate. The melting points, decomposition temperatures and electrochemical stabilities of the salts were measured by DSC, TGA, and CV, respectively. The salts with halide anion showed high melting points ($150{\sim}200^{\circ}C$), low decomposition temperatures ($200{\sim}230^{\circ}C$), narrow electrochemical stabilities (3.4~3.6 V). The synthesized salts with inorganic anions, on the other hand, presented low melting point ($50{\sim}110^{\circ}C$), high decomposition temperatures ($250{\sim}380^{\circ}C$), wide electrochemical stabilities (6.1~6.3 V). We also found that the properties depend on the length of the carbon chain.