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Inhibitory Effect of Glycoprotein Isolated from Cudrania tricuspidata Bureau on Histamine Release and COX-2 Activity in RBL-2H3 Cells (RBL-2H3 세포에 있어서 꾸지뽕 당단백질에 의한 히스타민 방출 및 COX-2 활성 억제 효과)

  • Oh, Phil-Sun;Lee, Hye-Jin;Lim, Kye-Taek
    • Korean Journal of Food Science and Technology
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    • v.41 no.4
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    • pp.405-412
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    • 2009
  • The purpose of this study was to determine the inhibitory effect of a glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein, 75 kDa) on immunoglobulin E (IgE)-induced allergic inflammation in RBL-2H3 cells. This experiment evaluated the production of intracellular reactive oxygen species (ROS), the activities of mitogenactivated protein kinase (MAPK), transcription factor (c-jun), and cyclooxygenase (COX)-2, and histamine release in cells. The results showed that the CTB glycoprotein inhibited histamine release and COX-2 expression induced by IgE in the cells. The CTB glycoprotein also had suppressive effects on the expressions of ERK1/2, p38 MAPK, c-jun, and the production of intracellular ROS in IgE-treated RBL-2H3 cells. The activities of c-jun and COX-2 were collectively blocked by ERK1/2 inhibitor (PD98059) and p38 MAPK inhibitor (SKF86002), respectively. Hence, we speculate that CTB glycoprotein might be a component with potential use in the preparation of health supplements for the prevention of allergic diseases.

Conditions for Obtaining Optimum Polyphenol Contents and Antioxidant Activities of Korean Berry and Green Tea Extracts (반응표면분석을 이용한 오가자, 오디, 복분자 및 녹차의 항산화 활성 추출 최적화)

  • Lee, Ji-Hye;Kim, Yang;Lee, Suyong;Yoo, Sang-Ho
    • Korean Journal of Food Science and Technology
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    • v.46 no.4
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    • pp.410-417
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    • 2014
  • Berries and green tea are underutilized in the food industry despite their great potential as a functional food ingredients. The purpose of this study was to determine the extraction conditions under which total phenolic contents and antioxidant activities of berry and green tea extracts are maximized. Extracts produced using 0-80% ethanol and temperatures ranging from $5-65^{\circ}C$ were evaluated for total phenolic content (TP), as well as for DPPH and ABTS radical-scavenging activities by using response surface methodology. Both ethanol and temperature had significant effects (p<0.05). Ogaja extract produced at $67^{\circ}C$ by using 33% ethanol yielded maximum TP, ABTS, and DPPH values of 23.74 mg GAE/g, 19.77, and 25.04 mg VCE/g, respectively. Optimum conditions for mulberry and raspberry extraction were found to be $65^{\circ}C$ by using 69% and 40% ethanol, respectively. Mulberry and raspberry extracts had TP, DPPH, and ABTS values of 20.74 mg GAE/g, 23.55, and 35.44 mg VCE/g, and 26.08 mg GAE/g, 39.93, and 55.60 mg VCE/g, respectively. Green tea extraction at $57^{\circ}C$ by using 43% ethanol was found to be optimal, yielding TP, ABTS, and DPPH values of 101.15 mg GAE/g, 171.38, and 177.56 mg VCE/g, respectively.

Histological analysis of explanted implant-bone interface: a case report (임플란트 매식체 파절로 제거된 임플란트 골계면의 조직학적 분석 증례)

  • Kim, Dae-Dong;Kang, Dae-Young;Cho, In-Woo;Song, Young-Gyun;Shin, Hyun-Seung;Park, Jung-Chul
    • Journal of Dental Rehabilitation and Applied Science
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    • v.35 no.4
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    • pp.235-243
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    • 2019
  • Osseointegration has been reported to be a dynamic process in which the alveolar bone comes in direct contact with the implant. Various methods were tried to evaluate degree of osseointegration and the measurement of bone-implant contact (BIC) have been commonly used among them. To properly assess the BIC, only histologic analysis is available. However, few studies evaluated BIC of successfully osseointegrated implants in humans. Thus, this is a unique opportunity when implants should be explanted due to inappropriate positioning of implant, presence of pain or sensory disturbance, or broken screw or fixture. This report presents a case of the implant underwent 3-year functional load and a histologic analysis after the fixture fracture. The histomorphometric analysis revealed 53.1% of BIC measured along the whole implant and 70.9% measured only in subcrestal area, respectively. In the present study, although the implant was fractured, a high degree of BIC was observed.

Comparative analysis of various corrosive environmental conditions for NiTi rotary files (니켈티타늄 파일의 부식에 영향을 미치는 다양한 환경 조건 비교)

  • Yum, Ji-Wan;Park, Jeong-Kil;Hur, Bock;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.33 no.4
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    • pp.377-388
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    • 2008
  • The aim of the present study is to compare the corrosion tendency using two kinds of NiTi files in the various environmental conditions through the visual examination and electrochemical analysis. ProTaper Universal S2, 21 mm (Dentsply Maillefer, Ballaigues, Switzerland) and Hero 642, 0.06 tapers, size 25, 21 mm (Micromega, Besancon, France) rotary instruments were tested. The instruments were randomly divided into eighteen groups (n = 5) by the immersion temperature, the type of solution, the brand of NiTi rotary instrument and the presence of mechanical loading. Each file was examined at various magnifications using Scanning Electron Microscope (JEOL, Akishima, Tokyo, Japan) equipped with energy dispersive X-ray microanalysis (EDX). EDX was used to determine the components of the endodontic file alloy in corroded and noncorroded areas. The corrosion resistance of unused and used NiTi files after repeated uses in the human teeth was evaluated electrochemically by potentiodynamic polarization test using a potentiostat (Applied Corrosion Monitoring, Cark-in-Cartmel, UK). Solution temperature and chloride ion concentration may affect on passivity of NiTi files. Under the conditions of this in vitro study, the corrosion resistance is slightly increased after clinical use.

A Study on Material Expression and Symbolism of Carlo Scarpa's Garden Details (카를로 스카르파(Carlo Scarpa)의 정원 디테일에 나타난 재료 표현기법 및 상징성 연구)

  • Lee, Hyung-Sook
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.36 no.2
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    • pp.54-60
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    • 2018
  • The purpose of this study is to analyze the garden details of Carla Scarpa in order to understand his selection and composition of materials, detailing style and symbolism of the spaces. Literature review and a field trip were conducted for the study and the results are as follows. First, Scarpa used vernacular materials such as Murano glass and Istrian limestone, and juxtaposed various materials using contrast of color and texture. His mixed uses of traditional and modern materials shows the passage of time. Second, he create his own detail style such as ziggurat and geometric motif, which make the garden space to look more interesting and rich. Scarpa respected local craftsmanship like glass design and used textile design style such as overlaying. Third, symbolic uses of water features help make narrative and poetic gardens. Scarpa's unique detail style and respects for traditional craftsmanship provide lessons on how to interpretate traditional design style in modern garden.

Cancellation of MRI Artifact due to Rotational Motion (회전운동에 기인한 MRI 아티팩트의 제거)

  • 김응규
    • Journal of KIISE:Software and Applications
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    • v.31 no.4
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    • pp.411-419
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    • 2004
  • When the imaging object rotates in image plane during MRI scan, its rotation causes phase error and non-uniform sampling to MRI signal. The model of the problem including phase error non-uniform sampling of MRI signal showed that the MRI signals corrupted by rotations about an arbitrary center and the origin in image plane are different in their phases. Therefore the following methods are presented to improve the quality of the MR image which includes the artifact. The first, assuming that the angle of 2-D rotational motion is already known and the position of 2-D rotational center is unknown, an algorithm to correct the artifact which is based on the phase correction is presented. The second, in case of 2-D rotational motion with unknown rotational center and unknown rotational angle, an algorithm is presented to correct the MRI artifact. At this case, the energy of an ideal MR image is minimum outside the boundary of the imaging object to estimate unknown motion parameters and the measured energy increases when the imaging object has an rotation. By using this property, an evaluation function is defined to estimate unknown values of rotational angle at each phase encoding step. Finally, the effectiveness of this presented techniques is shown by using a phantom image with simulated motion and a real image with 2-D translational shift and rotation.

Transmission Noise Seduction Performance of Smart Panels using Piezoelectric Shunt Damping (압전감쇠를 이용한 압전지능패널의 전달 소음저감 성능)

  • 이중근
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.3 no.1
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    • pp.49-57
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    • 2002
  • The possibility of a transmission noise reduction of piezoelectric smart panels using piezoelectric shunt damping is experimentally studied. Piezoelectric smart panel is basically a plate structure on which piezoelectric patch with shunt circuits is mounted and sound absorbing materials are bonded on the surface of the structure. Sound absorbing materials can absorb the sound transmitted at mid frequency region effectively while the use of piezoelectric shunt damping can reduce the transmission at resonance frequencies of the panel structure. To be able to reduce the sound transmission at low panel resonances, piezoelectric damping using the measured electrical impedance model is adopted. Resonant shunt circuit for piezoelectric shunt damping is composed of register and inductor in series, and they are determined by maximizing the dissipated energy throughout the circuit. The transmitted noise reduction performance of smart panels is investigated using an acoustic tunnel. The tunnel is a tube with square crosses section and a loud-speaker is mounted at one side of the tube as a sound source. Panels are mounted in the middle of the tunnel and the transmitted sound pressure across panels is measured. Noise reduction performance of a smart panels possessing absorbing material and/or air gap shows a good result at mid frequency region but little effect in the resonance frequency. By enabling the piezoelectric shunt damping, noise reduction of 10dB, 8dB is achieved at the resonance frequencise as well. Piezoelectric smart panels incorporating passive method and piezoelectric shunt damping are a promising technology for noise reduction in a broadband frequency.

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A study on Estimation of CO2 Abatement Cost in Korean Offshore Fishery (우리나라 근해어업의 CO2 배출 저감비용함수 추정)

  • Shin, Yongmin;Jeong, Gyeowoon
    • Environmental and Resource Economics Review
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    • v.27 no.3
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    • pp.399-420
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    • 2018
  • This study has attempted environmental economic analysis on the cost structure of offshore fisheries based on fishery management data published by the Fisheries Research Institute to examine the effect of the environmental policy on the fisheries for the effective implementation of the Paris Convention. Under the assumption that both fisheries and carbon dioxide are simultaneously produced, the cost structure of offshore fisheries were analyzed. Cost function in a translog form was estimated and SUR (Seemingly Unrelated Regression) model was used for the analysis. Here, $CO_2$ emission of offshore fishery was calculated by using National Federation of Fisheries Cooperatives' data on supply of tax exemption oil (2003~2016). The cost function estimation showed that there is a weak disposition between catches and $CO_2$ emissions during the sample period, and the marginal abatement cost (MAC) is estimated at 1,457 won per year. In addition, for the same period, when 1% of $CO_2$ per horsepower is to be reduced MAC increases by 2.2%, and when 1% of $CO_2$ per 1 ton of catch is to be reduced, MAC increases by 1.4%.

An Experimental study to estimate physical properties of porous media by a permittivity method (유전율법에 따른 다공질 매질의 특성 파악을 위한 실험적 연구)

  • 김만일;니시가끼마코토
    • The Journal of Engineering Geology
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    • v.13 no.4
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    • pp.405-418
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    • 2003
  • Measurements of volumetric water content and saturation of porous media are very important factors in understanding the physical characteristics of soil, groundwater recharge by rainfall, pollutant movement, and slope failure. To measure such physical parameters, a permittivity method using electromagnetic wave is applied and use is made of the special permittivity response of understand to water and ethanol. In particular, the estimation is required because permittivity is influenced by the nature of the underground environment. In this study, we carried out experiments on the relative dependency of soil density, temperature and salinity of standard sand and granitic weathered soil using FDR-V system (Frequency domain reflectometry with vector network analyzer) within a frequency range of 1 - 18 GHz. The results of the study showed that the dielectric constants of standard sand and granitic weathered soil increased with increased volumetric water content of soil. However, the dependency of soil density was found to be a little low. Changes of dielectric constant with temperature appeared definitely in the real part of 1 GHz. That is, the dielectric constant of real part at 1 GHz of water and standard sand increased with the rise of temperature. However, ethanol showed decreased tendency. The study also showed that dielectric constant increased with increase in salinity at imaginary part of 1 GHz. It could be concluded from this study FDR-V system can adequately measure the physical properties of soil and the degree of salinity concentration of porous media within 1 GHz frequency range using dielectric constant.

A Solute Transport Analysis around Underground Storage Cavern by using Eigenvalue Numerical Technique (고유치 수치기법을 이용한 지하저장공동 주위의 용질이동해석)

  • Chung, Il-Moon;Kim, Ji-Tae;Cho, Won-Cheol;Kim, Nam-Won
    • The Journal of Engineering Geology
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    • v.18 no.4
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    • pp.381-391
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    • 2008
  • The eigenvalue technique is introduced to overcome the problem of truncation errors caused by temporal discretization of numerical analysis. The eigenvalue technique is different from simulation in that only the space is discretized. The spatially discretized equation is diagonized and the linear dynamic system is then decoupled. The time integration can be done independently and continuously for any nodal point at any time. The results of eigenvalue technique are compared with the exact solution and FEM numerical solution. The eigenvalue technique is more efficient than the FEM to the computation time and the computer storage in the same conditions. This technique is applied to the solute transport analysis in nonuniform flow fields around underground storage caverns. This method can be very useful for time consuming simulations. So, a sensitivity analysis is carried out by using this method to analyze the safety of caverns from nearly located contaminant sources. According to the simulations, the reaching time from source to the nearest cavern may takes 50 years with longitudinal dispersivity of 50 m and transversal dispersivity of 5 m, respectively.