• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.38-43
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• 2014

To establish the system of in vitro plant regeneration, the different explants (stem with axillary bud and stem without axillary bud) of Hylotelephium ussuriense were cultured on the Murashige and Skoog's medium containing 6-benzylaminopurine (BA) and indolebutyric acid (IBA). The adventitious shoot induction was more effective in the stem with axillary bud explants than the stem without axillary bud explants, and was the best on MS medium containing 3.0 mg/L BA and 0.01 mg/L IBA. Frequency of plantlet growth was not significantly treated on MS and sucrose. Total chlorophyll contents under ventilation treatment were higher than those in control (non-ventilation). This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• Analytical Science and Technology
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• v.18 no.4
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• pp.271-277
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• 2005

Urea in blood has been measured as an effective marker for diagnosis of renal function. Urea which is e end-product of nitrogen containing metabolites such as proteins is filtered through glomeruli of kidneys and then excreted as urine. If the renal function is deteriorated, the urea concentration in blood will be increased, from which the healthiness of renal function is judged. In order to improve the confidence of diagnosis results, the results must keep traceability chain to certified reference materials, which was certified by primary reference method. In this study, we proposed isotope dilution-liquid chromatography/mass spectrometry (ID-LC/MS) as a candidate primary method, in which $15^N_2$-urea is used as an internal reference material. The developed method is highly accurate in principle and is convenient as it does not require cumbersome derivatization. 0.1 mmol/L ammonium chloride was selected as a mobile phase for HPLC because it provided low interference in MS analysis of relatively low molecular weighted urea. HPLC and MS were connected with an electrospray ionization (ESI) interface of positive mode, which provided high sensitivity and reproducibility. The developed method was validated with internationally recognized reference materials, and we have obtained satisfactory results in an international ring trial. The expanded uncertainty calculated according to ISO guide was 1.8% at 95% confidence interval. The developed method is being used as a primary reference measurement method such as for certification of serum certified reference materials (CRMs).

• 월간산업보건
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• s.380
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• pp.8-15
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• 2019

황색인(Yellow phosphorus)에 대한 직업적 노출기준 TLV-TWA는 0.1 mg/㎥(0.02ppm)으로 권고하였다. 이 수준은 보고된 간경변증을 포함하여 호흡기 자극, 급성 중독인 전해질 불균형, 심근 붕괴 및 신장 피질 괴사의 가능성을 최소화하기 위한 것이다. 그러나 제한된 자료로 인중독성괴저(phossy jaw)와 같은 만성적 영향에 대한 보호 한계 수준을 설정하기에는 불확실하다. 입자 상태의 인(phosphorus)에 대한 노출은 예상되지만 증기압 수준을 감안할 때 증기에 대한 직업적 노출의 유해성을 내포하고 있다. 황색인은 가장 독성이 강한 무기 물질 중에 하나이다. 또한, 결정체 고체(crystalline solid)는 30℃ 이상의 온도에서 공기 중에 자발적으로 발화될 수 있으며, 독성이 높은 흄도 방출될 수 있다.

• Proceedings of the KSCN Conference
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• 2004.05a
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• pp.417-417
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• 2004

지속성복막투석환자의 경우 투석에 의해 단백질 대사물질의 배설은 효과적으로 이루어지나 오랫동안의 만성신부전으로 인한 인의 배설 저하에 따라 골밀도가 대부분 저하되어 있으며 신성골이영양증을 수반하는 경우가 많다. 본 연구에서는 종합병원 신장내과 클리닉에 정기적 checkup을 받고 있는 지속성복막투석환자를 대상으로 골밀도를 측정하였으며 요추(L2-L4)와 대퇴부(femoral neck, trochanter)의 평균 골밀도의 T값이 모두 -1 미만인 군을 골감소증군으로 하였고(N=86) 1군데라도 T값이 -1 이상인 군은 정상군으로(N=32) 분류하여 영양소섭취량, 식행동, 우울정도에 따른 차이를 보고자 하였다.(중략)

• Journal of the korean Society of Automotive Engineers
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• v.26 no.3
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• pp.39-43
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• 2004

최신의 엔진기술과 배기 후처리 기술만으로는 앞으로 더욱 엄격해질 일본의 신장기 규제치(2005년)를 만족시키기에 한계를 느끼기 시작함으로서 배출가스 저감에 보다 효과적인 대응책이라고 할 수 있는 연료설계가 주목을 받고 있다. 그 대표적인 예로서 천연가스, 석탄 및 바이오 물질을 이용해 제조가 가능한 GTL(Gas to Liquid), DME(Dimethyl Eter) 및 바이오 연료는 석유의 대체연료 뿐만 아니라 유황 및 방향족 성분이 매우 낮은 저공해 연료로도 일본 내에서 연구가 활발히 이루어지고 있다. (중략)

• Electrical & Electronic Materials
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• v.4 no.3
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• pp.229-238
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• 1991

본 논문에서는 영상센서를 이용하여 유기절연물질내의 트리 열화현상을 측정할 수 있는 시스템에 관하여 논하였다. 기존의 육안관찰법으로서는 관측하는 것이 매우 어려웠던 트리형상의 변화를 화상처리 기술을 이용하므로서 트리발생 직후부터 관측할 수 있게 되었다. 실험결과, 화상처리장치는 최초로 발생된 트리에서 부터 그 진전상황을 즉각적으로 기록할 수가 있었으며 트리의 신장도, 열화면적 및 연속 화상처리 측면에서 트리의 진전특성을 고찰하였다.

• Microbiology and Biotechnology Letters
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• v.5 no.1
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• pp.36-45
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• 1977

A piscicidal substance was isolated from the culture medium of Streptomyces umbrosus by avicel column chromatography and avicel thin layer chromatography after extration with chroloform. Bluegreen fluorescence was emitted under UV irradiation. Factors which govern toxin production and nutrition requirement for high toxin titres were observed. Nutritional uptake for toxin production was not curresponded with that for cell growth. Alanine, valine, serine asparagine, arginine, histidine, urea and sodium nitrate as a carbon source and glucose, mannose, rhamnose, xylose, arabitol and starch as a carbon source were recognized as a favorable nutrient for high toxin production. Magnesium was essential factor whereas vitamins were not of effective. Most of toxin was formed simultaneously with cell growth in esponential phase. Maximal production was observed for six day culture at 3$0^{\circ}C$. Tissues of gill, kidney and pnacreas in Cyprinus carpio were denatured extreamly after treating with the substance. Atrophied nucleous, indented membrane and degradated cytoplasm with necrotic affectness were noted on each tissue. The chemical formula of the substance was designated as $C_{38}$ $H_{66}$ $NO_4$.

• The Safety technology
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• no.190
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• pp.24-26
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• 2013

최근 화학물질 안전사고가 잇따라 발생하면서 사업장 내 안전제품을 구비하고 안전한 작업환경을 조성하려는 움직임이 확산되고 있다. (주)유민에쓰티(대표 유흥근)는 이러한 화학물질 안전사고를 예방하기 위해 불산, 염소 등의 화학약품 유출을 감지하는 센서장치를 생산하는 곳이다. 이곳은 누수감지센서를 비롯해 스팀검출센서, 옥외용 강산 검출센서, 유기성액체 검출센서 등을 개발하면서 산업안전제품시장에서 무서운 신장세를 드러내고 있다. 특히 지난 2008년에는 인쇄전자 기반의 필름형 누수감지센서를 세계 최초로 개발해 국무총리상을 수상하면서 우수한 기술력을 자랑하기도 했다. 또한 국내외 약 50여 건에 이르는 특허 획득을 통해 품질을 인증받았으며, 미국전기전자공학회(IEEE)에 소개되는 등 해외에서도 기술력을 인정받고 있다. 이처럼 (주)유민에쓰티는 끊임없는 기술 개발로 안전한 작업현장을 조성하는 데 이바지하고 있다. 하지만 유민에스티의 모든 관계자들은 사업장 내 안전이 먼저 확보됐었기에 이 같은 성장이 가능했다고 입을 모아 말한다. 제조업 특성상 안전사고 발생률이 높은데도 불구하고 2003년 설립된 이후 단 한 건의 안전사고와 산재가 발생하지 않았을 만큼 철저한 안전관리를 전개하고 있는 것이다. 안전을 바탕으로 눈부신 성장을 하고 있는 (주)유민에쓰티를 찾아가봤다.

• Childhood Kidney Diseases
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• v.2 no.2
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• pp.138-144
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• 1998

Purpose : The use of cyclosporine and mitomycin in various immunologic or neoplastic disorders has been known to cause wide-ranged nephrotoxic effects including thrombotic microangiopathy. However, the mechanism of nephrotoxicity of these drugs has not been studied adequately, so that present experimental study has been undertaken to find out whether these drugs can cause direct damage to the kidney and to clarify the pathogenetic mechanism of nephrotoxic effect of these drugs. Materials and methods : Sprague-Dawley rats weighing 250-300 gm were used for experimental animals and unilateral renal perfusion technique, modified from the method described by Hoyer et al was used. Isolation of left kidney from systemic circulation was made by clamping aorta and left renal vein and a hole was punctured in the anterior wall of the left renal vein. Cyclosporine (2.5 mg in 4 ml solution) and mitomycin (1.6 mg in 4ml solution) were infused through left renal artery and normal saline was used in control rats. Forty-eight hours after infusion of the drugs, animals were sacrificed and left kidney removed and processed for histologic examination. Total ischemic time of left kidney was less than 15 minutes: Results : Cyclosporine-perfused group showed severe swelling of glomerular endothelial ceil along with swelling of glomerular epithelial cell and interstitial vascular endothelial cell. Mitomycin-perfused group also showed severe swelling of glomerular endothelial and epithelial cells. And in addition to these findings, they demonstrated platelets aggregation, swelling and degranulation of platelets and fibrin accumulation in some of the capillaries, indicating occurrance of thrombotic microangiopathy. Conclusion : present experiment indicates that cyclosporine and mitomycin can cause direct toxic injury to renal endothelial cell. And this direct toxic damage to endothelial cell seems to be an important initiating event for the development of thrombotic microangiopathy.

• The Korean Journal of Nuclear Medicine Technology
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• v.19 no.1
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• pp.37-43
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• 2015

Purpose $^{99m}Tc-DMSA$ renal scintigraphy serves as location, size and shape of kidney, so it has been used for diagnosis and passage observation after the operation or treatment. There are 3 methods of calculating the relative renal uptake ratio such as geometric mean of the counts from the anterior and posterior views, arithmetical mean from the only posterior view and posterior view which applied the renal depths. In this study, we seek to correlation between the change of total relative uptake ratio according to different inspection methods of obtaining the renal count rate. Materials and Methods The phantom experiments proceeded 5 times depending on each renal depth with the kidney phantom and tissue equivalent materials. In the clinical research, we investigated 36 adult patients who had visited our hospital from february to october, 2014 and received $^{99m}Tc-DMSA$ renal scan. The equipment was used as a gamma camera named INFINIA (General Electric Healthcare, milwaukee, USA) and we drew the region of interests through semiautomatic method by using Xeleris Ver. 2.1220 of GE. In addition, we obtained the lateral view of kidney to measure the renal depth of each patient. Then the results were compared with 3 methods of calculating relative renal uptake ratio. Results The phantom studies show when the difference between the left ant right kidney depth were less than 1 cm, there were no statistically significant difference among values calculated through anterior and posterior views and only posterior view (P>0.05), while the excess of 1cm, the results showed a statistically significant change in the value (P<0.05). In case of clinical research, the correlation between total relative uptake ratio by obtaining both sides of image and posterior view applied the kidney depth (r=0.999) was higher than by obtaining only posterior view and applying the kidney depth to one side image (r=0.988). Conclusion This study has found that, the difference of calculating total relative uptake ratio compared with obtaining anterior and posterior views and only posterior view. In order to reduce the error, we recommend the method of obtaining anterior and posterior views and is considered to be useful, particularly the patients have similar uptake ratio of left and right kidney and difficulties of measurements of kidney depth.