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Changes in Quality Characteristics of Garlic Jam during Storage (마늘 잼의 저장과정 중 성상변화)

  • Park, Hye-Min;Kwak, Hyo-Soon;Kim, So-Hee;Moon, Mi-A;Oh, Man-Jin
    • Korean Journal of Agricultural Science
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    • v.35 no.2
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    • pp.183-191
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    • 2008
  • In order to make a functional jam containing garlic, sugar, acid and pectin were mixed with garlic and concentrated by heating the mixture under atmosphere or vacuum conditions. And changes of some physico-chemical properties of the garlic jam(i.e., color, textural properties, vitamin C) were investigated during storage at $30^{\circ}C$. It was found that lightness(L) was higher in sample of vacuum concentration than that in atmosphere concentration and redness(a) and yellowness(b) were higher in sample of atmosphere concentration. During the storage lightness was found to decrease for all samples with more drastic decrease in sample of atmosphere concentration where browning was more severely observed after 8 weeks of storage. In terms of textural properties, hardness, chewiness and gumminess were higher in sample of atmosphere concentration than that in vacuum concentration. And springiness and cohesiveness were higher in sample of vacuum concentration. Although sensory score for garlic jam found to be lower than that for the existing strawberry jam, the present garlic jam might have high marketability if considering its physiological value.

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Crystal Growth of rutiles doped with Impurity Ions by Floating Zone Method (부유대용융법에 의한 불순이온 주입된 $TiO_2$단결정 성장 연구)

  • 이성영;유영문;김병호
    • Proceedings of the Korea Crystallographic Association Conference
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    • 1999.04a
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    • pp.1-1
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    • 1999
  • 부유대용융법에 의하여 불순이온의 종류와 각 이온의 주입 농도를 달리하는 Rutile 단결정을 성장하였다. 성장된 결정으로부터 제조한 박편시료를 이용하여 결정결함과 광투과도에 미치는 각 불순이온의 영향을 조사하였다. 결정성장용 주원료로 99.99%의 TiO2를 사용하고, 불순이온 주입을 위한 원료로서 99.99%의 Al2O3, H3BO3, Ga2O3, Sc2O3, V2O5, Fe2O3, ZrO2, Er2O3, Cr2O3를 각각 사용하였다. 불순이온의 종류에 따르는 영향을 조사하기 위하여 TiO2 99.8 atomic%-불순이온 0.2atomic%의 조성이 되도록 각 이온별로 원료를 정밀하게 평량하고 균일 혼합하였다. 불순 이온의 첨가량에 따르는 영향을 조사하기 위하여 Al2O3는 각각 pure, 0.2, 0.4, 0.6 atomic%를, Cr2O3는 pure, 0.003, 0.05, 0.2 atomic%를 각각 치환하여 원료를 조합하였다. 균일 혼합된 원료를 직경 8mm의 고무 튜브에 넣고 CIP(Cold Isostatic Press0에서 2000kg/$\textrm{cm}^2$의 압력으로 성형한 후 150$0^{\circ}C$에서 1시간 소결함으로서 결정성장용 다결정 원료를 합성하였다. 합성된 다결정을 double ellipsoidal mirror 내에 설치하고,halogen lamp로 가열하여 원료의 한쪽 끝을 용융한 다음, 20rpm의 회전속도, 3-5mm/hr의 성장속도로 하는 유속 1$\ell$/min의 O2 분위기속에서 부유대용융법에 의하여 결정을 성장하였다. 성장된 결정을 성장축에 수직한 방향으로 각각 절단, 연삭, 연마한 박편을 이용하여 편광하에서 low-angle grain boundaries 및 기타의 결정결함을 관찰하였으며, 0.3$\mu\textrm{m}$~0.8$\mu\textrm{m}$ 범위 및 0.6$\mu\textrm{m}$~3.4$\mu\textrm{m}$ 범위에서의 투과 및 흡수 스펙트럼을 측정하였다. 결정 성장 결과 B3+, Er3+, Cr3+ 이온은 Ti4+ 이온과 이온의 크기 차이가 심하여 결정의 정상적인 성장을 방해하는 물성을 나타냈고, V5+, Cr3+ 이온은 흑색의 결정, Fe3+ 이온은 적갈색의 결정으로 성장되었다. Al3+, Zr4+, Al3+의 순서로 투과도가 높아지는 것이 관찰되었다. 불순이온의 농도에 따른 영향으로서 Al3+ 이온의 경우 주입농도가 높아질수록 low angle boundary와 oxygen deficiency가 감소하였고, 투과율은 조금 감소하거나 큰 차이가 없는 것으로 나타났다. 반면에 Cr3+ 이온을 주입한 경우 0.003 atomic%에서 최적의 물성을 보였으며, 주입농도가 높아질수록 결정성장이 어려워지고 광의 투과도가 급격히 저하되었다.

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Drug Resistance of Mycobacterium Tuberculosis in Korea (한국인 결핵환자로 부터 톨리된 인형결핵균의 약제내성)

  • Kim, Sang-Jae;Hong, Young-Pyo;Han, Yong-Chul;Kim, Sung-Jin
    • Tuberculosis and Respiratory Diseases
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    • v.38 no.2
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    • pp.99-107
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    • 1991
  • Drug resistance of M. tuberculosis has been investigated with isolates from patients screened out of the sample population of the nationwide tuberculosis prevalence surveys or from the routine cultures. The results showed a close inverse relationship between prevalence of drug resistance and efficiency of the past or current treatment regimens of NTP. Individual drug resistance also showed a close relationship with the extent of use of the relevant drugs. Drug resistance was found in 38.0% of M. tuberculosis isolates from patients found in 1965 survey and remained unchanged until it increased upto 48.0% in 1980 survey. The resistance prevalence, however, dropped to 30.8% in 1985 and further to 25.3% in 1990 survey. Such decrease was fairly well coincided with a continuous increase of the treatment efficiency (from 60% in 1984 to 77% in 1989) in 1980s. Initial drug resistance also showed a similar trend, namely 26.2% in 1965, 23.9% in 1970, 20.1% in 1975, 30.6% in 1980, 17.4% in 1985, and 15.0% in 1990. The similar figures were observed in M. tuberculosis isolates from patients diagnosed in the routine services. Higher prevalence of initial drug resistance was observed among urban patients than rural patients and among young patients than old patients. These findings signify that a continuous survey on drug resistance permits to monitor efficiency of treatment programme of the country.

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POSTNATAL DEVELOPMENT OF CGRP-IMMUNOREACTIVE NERVE DISTRIBUTION OF RAT CIRCUMVALLATE PAPILLA (출생후 성장발육에 따른 흰쥐 유곽유두의 CGRP 면역양성 신경분포변화)

  • Lee, Jae-Mun;Kim, Hyun-Jung;Bae, Yong-Chul;Kim, Young-Jin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.24 no.2
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    • pp.339-351
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    • 1997
  • The purpose of this study was to investigate the distribution of nerves in the rat circumvallate papilla by each developing stage. The distribution of nerves in the rat circumvallate papilla were investigated by means of immunohistochemistry for detection of calcitonin gene-related peptide(CGRP). The results were as follows : CGRP-immunoreactive(IR) nerve fibers entered the base of the papilla laterally to form a subepithelial plexus. On the 1st postnatal day, the bead-like appearance, typical appearance of CGRP-IR nerve fiber, was seen, but from the 5th postnatal day the bead-like appearance was seen less clearly and nerve fibers looking like a line were often observed. Mature taste buds having taste pores were first seen at the 10th postnatal day and then, increased markedly after this stage. CGRP-IR nerve fibers entering the epithelium were rarely seen on the 5th postnatal day but they increased in number on the 10th postnatal day when mature taste buds having taste pores were firstly observed and then on the 15th postnatal day, they were observed abundantly throughout entire epithelium. But from the 20th postnatal day, the nerve fibers in the lower two-third of the trench wall in which taste buds exist decreased but on the top surface and upper one-third of the trench wall the nerve fibers were observed limitedly.

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Research on Measurement Condition Establishment of a Liquid Scintillation Counter System (액체섬광계수기 장비의 계측조건 확립에 관한 연구)

  • Park, Eung-Seop;Han, Sang-Jun;Lee, Seung-Jin;Kim, Hee-Gang;Lee, Na-Young;Mun, Ji-Yeon
    • Journal of Radiation Protection and Research
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    • v.31 no.3
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    • pp.155-164
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    • 2006
  • It is judged that there could be certainty in every process of analyzing environmental samples using Liquid Scintillation Counter. Therefore, this study focuses on quantitative evaluation on uncertainty in an effort to analyze comparatively accurately environmental samples. For this, after parameters which can have an effect on uncertainty was derived, the evaluation on each parameter was tamed out. The results of analysis of each parameter showed that the effect according to the weight difference of Teflon vial did not appear, and that standard deviations of SQP(E) averages reached saturation point at $75{\sim}90$ sec at the result of making increases step by step the irradiation time of External standard, and that values measured by repeat method produces good results compared with replicate. Also, conclusion was derived that analysis on sample after it is left in cold and dark room at least above 1,000 minutes have to be carried out, and the result of carrying out verification on results measured as well as equipment itself using radioactivity-error-analysis and chi-square test, resonable result was derived.

Tritium Distribution in Some Environmental Samples-Rices, Chinese Cabbages and Pine Needles in Korea (국내 환경시료(쌀, 채소, 솔잎) 중 삼중수소의 분포)

  • Kim, Chang-Kyu;Cho, Yong-Woo;Han, Man-Jung;Pak, Chan-Kirl
    • Journal of Radiation Protection and Research
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    • v.17 no.2
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    • pp.25-35
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    • 1992
  • To evaluate tritium level in some environmental samples, tissue-free water tritium (TFWT) and tissue-bound tritium (TBT) were analyzed in rices, chinese cabbages and pine needles collected at 12 locations in Korea. The TFWT was recovered by freeze-drying of the samples and the TBT was obtained in the form of water by combustion of the dried samples. Tritium was measured by liquid scintillation counter. The concentrations of TFWT were in the range of $0.96{\sim}3.96 Bq/1,\; 0.83{\sim}3.40 Bq/1\;and\;1.02{\sim}3.01 Bq/1$ in rices, chinese cabbages and pine needles, respectively. The mean specific activity ratios (TBT/TFWT) were 0.94, 1.71 and 1.39 in rices, chinese cabbages and pine needles, respectiviely. This excess TBT in the samples may be attributed to the fact that the residence time of TBT in the plant is longer than that of TFWT. The specific activity ratio depends on the plant species, the exposed time to tritiated atmosphere, atmospheric moisture, temperature and diffusion factor.

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Expression of Fra1 and Fra2 Genes are regulated by Estrogen in the Mouse Uterus (생쥐자궁에서 에스트로겐에 의해 조절되는 Fra1과 Fra2 유전자의 발현양상)

  • Lee, Ji-Yoon;Hong, Seok-Ho;Nah, Hee-Young;Kim, Sung-Hoon;Chae, Hee-Dong;Kim, Chung-Hoon;Kang, Byung-Moon;Kim, Moon-Kyoo
    • Clinical and Experimental Reproductive Medicine
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    • v.30 no.4
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    • pp.309-316
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    • 2003
  • 연구목적: 스테로이드 계통의 에스트로겐 호르몬은 막 수용체와 결합하고 DNA에 부착되어, 자궁조직에서 발현되는 많은 유전자들의 발현 양상을 조절하는 것으로 알려져 있다. 본 연구에서는 난소를 제거한 생쥐 모델을 이용하여 에스트로겐에 의해 조절되는 전사 관련 유전자(transcription factor)들을 동정하고, early up-regulation gene으로 확인된 Fos related antigen (Fra1과 Fra2) 유전자의 발현 양상을 RT-PCR과 면역염색방법으로 살펴보았다. 연구재료 및 방법: 난소 절제술을 시행한 생쥐에 에스트로겐을 피하주사하고 2, 4, 6, 12시간 간격으로 자궁조직을 적출하였다. 대조군으로는 sesame oil만을 주사한 후 2시간째에 수획한 자궁조직을 사용하였으며, 시간대별로 채취한 자궁조직(n=4)에서 RT-PCR을 수행하였다. RT-PCR을 통해 early response gene으로 확인된 Fra1과 Fra2에 대한 에스트로겐의 영향을 살펴보기 위해 estrogen receptor antagonist인 ICI 182, 780을 주사하여 유전자 발현 양상의 변화를 살펴보았다. 또한, 자궁조직내에서의 단백질 발현 부위를 관찰하기 위해 면역조직화학염색을 실시하였다. 결 과: 생쥐 자궁조직에서 에스트로겐에 의해 발현 양상의 변화가 확인된 유전자는 early up-regulation genes (CREB2, Fra-1, 2, GATA5), late up-regulation gene (E2F1), no response genes (CREB1, ATF1, GLI3, E2F3), down-regulation genes (GLI2, E2F5, GATA-2, 3, 6) 등으로 구분할 수 있었다. 그 중 early up-regulation genes에 해당하는 Fra1과 Fra2 유전자는 ICI 182, 780에 의해 그 발현이 유의하게 감소되는 것을 확인하였다(p < 0.01). 이들 단백질은 생쥐 자궁조직의 상피세포층, 기질층, 근육층에서 고루 발현되었으며, 특히 근육층에서 강한 염색정도를 관찰할 수 있었다. 결 론: 이상의 결과를 통해 Fra1과 Fra2 유전자의 발현은 에스트로겐에 의해 조절됨을 알 수 있었으며, 이들의 강한 발현이 자궁조직의 근육층에서 관찰되어 이들의 기능에 대한 연구가 필요할 것으로 생각된다.

Effect of Embryo Number and Incubation Volume on the Development of Pre- and Post-implantation Mouse Embryos In Vitro (배아밀도와 배양액 용량이 착상전후의 생쥐배아의 체외 성장에 미치는 영향)

  • Kang, Byung-Moon;Cheon, Yong-Pil;Kim, Ji-Young;Kim, Jeong-Hee;Lee, Ji-Yun;Chae, Hee-Dong;Kim, Chung-Hoon;Chang, Yoon-Seok;Mok, Jung-Eun
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.3
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    • pp.377-383
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    • 1997
  • The effects of embryo number and incubation volume on the development of mouse embryos were evaluated. The growth rate of two-cell mouse embryos to attached blastocyst stage and the growth rate of blastocysts to early somite stage were assessed after culture in different incubation volumes and embryo densities. Embryos were collected from ICR female mice superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin and mated by ICR males. In experiment 1, groups of one, five, ten, twenty 2-cell embryos were cultured in 10-, 50-, 500-, 1000-${\mu}l$ drops of BWW media under mineral oil at $37^{\circ}C$ in a humidified atmosphere of 5% $CO_{2}$ and 95% air. As the incubation volume decreased, significantly (p<0.05) higher rates of embryos reached morular and blastocyst stage on day 3 and 4 culture, respectively. In experiment 2, groups of one, five, ten, twenty blastocysts were cultured in 1- and 2-ml volumes of CMRL 1066 media under same condition as in experiment 1. However the reverse was the result. Decreasing the number of embryos incubated per volume from 1 to 20 significantly (p<0.05) increased the number of blastocysts reaching the late egg cylinder (LEC) and early somite (ES) stage on day 6 and 8 culture, respectively, regardless of incubation volume. Blastocysts cultured in 2ml had higher (p<0.05) development rates to LEC and ES stage on day 6 and 8 culture, respectively, than embryos cultured in 1ml. Our results suggest that the effects of embryo number and incubation volume on the development of mouse embryos are stage specific and the shifting point was between hatching and EEC stage.

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Preparation and Characteristics of Ipriflavone-Loaded PLGA Microspheres (이프리플라본을 함유한 생분해성 미립구의 제조와 특성분석)

  • 이진수;강길선;이종문;신준현;정제교;이해방
    • Polymer(Korea)
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    • v.27 no.1
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    • pp.9-16
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    • 2003
  • Ipriflavone (IP) stimulates proliferation and differentiation of osteoblast and also enhances calcitonin secretion in the presence of estrogen. Poly(lactide-co-glycolide) (PLCA) due to its controllable biodegradability and relatively good biocompatibility is one of the most significant candidates for the study of drug controlled release system. In this study, IP-loaded PLGA microspheres (MSs) was prepared by using conventional O/W solvent evaporation method. The size of MSs was in the range of 5~200 $mu extrm{m}$. The morphology of MSs was characterized by SEM. And, in vitro release amounts of IP were analyzed by HPLC. The highest encapsulation efficiency were obtained by using gelatin and polyvinyl alcohol (PVA) as emulsifiers. The morphology, size distribution, and in vitro release pattern of MSs were changed by several preparation parameters such as molecular weights (8, 20, 33 and 90 kg/mol), polymer concentrations (2.5, 5, 10 and 20%), emulsifier types (PVA, gelatin and Tween 80), initial drug loading amount (5, 10, 20 and 30%) and stirring speed (250, 500 and 1000 rpm). The release of IP in vitro was more prolonged over 30 days, with close to zero-order pattern by controlling the preparation parameters. The physicochemical properties of IP-loaded PLGA MSs were investigated by XRD and DSC.

Preparation and Actuation Performance of Ionic Polymer-Metal Composite Actuators Based on Nafion-Alumina Composite Membranes (나피온-알루미나 복합막을 사용한 이온성 폴리머-금속 복합체 작동기의 제작 및 성능 평가)

  • Lee, Jang-Woo;Kim, Woo-Sung;Yoo, Young-Tai
    • Polymer(Korea)
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    • v.33 no.4
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    • pp.377-383
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    • 2009
  • Ionic polymer-metal composite (IPMC) actuator generates bending actuation via ion/water flux to the cathode side under an electric field. Polyelectrolytes in IPMC should possess high water-retention capability, proton conductivity, and Young's modulus. In this study. for endowing IPMCs with these properties, Nafion-alumina composite membranes containing $\alpha$- or $\gamma$-aluminas of $4{\sim}8$ wt% were prepared. Mechanical moduli of Nafion-alumina composite membranes were $7{\sim}3$ MPa higher than that of Nafion, with the slight decrease in proton conductivity. At DC 3 V. the actuation performance of the Nafion-$\alpha$-alumina (8 wt%)-IPMC was superior to that of the typical Nafion-IPMC. exhibiting 2.7 times the displacement with an enhanced blocking force. The enhanced actuation performance with the Nafion-$\alpha$-alumina composite membranes was attributed to the higher proton conductivity, the elevated ion/water flux, and the lower interfacial electric resistance of platinum electrodes and membrane, compared with those containing $\gamma$-alumina.