• The Korean Journal of Soil Zoology
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• v.5 no.2
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• pp.125-131
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• 2000

Disposing technique for dairy cattle manure using earthworms and the possibility of utilizing earthworms in poultry feed were investigated. In addition, chemical properties of manure and cast of earthworms were investigated. Temperature in the vinyl-house for earthworm nursery was able to be maintained above 0$\^{C}$ in winter and 25-28$\^{C}$ in summer. In closed-covering method, the number of escaped earthworms was the highest among three covering methods. The time was longer after manure excretion, the numbers of escaped earthworm were higher. There were some incidences of mortality of earthworm in closed-covering site. The amount of manure ingested by earthworm was increased in order of non-covering, closed-covering and gap-covering method. Fresh manure was more ingested than old one by earthworm. Earthworms reduced manure about 50% on the dry weight base and reduced total amounts of N, P$_2$O$\_$5/ and K$_2$O of manure by 41, 50 and 60%, respectively. Earthworm could be utilized by adult poultry (>60 g) for feeding. However, adverse effect of live earthworm was observed with young poultry (<450 g).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.295-301
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• 2017

Botulinum neurotoxin (BoNT/A) is a neurotoxin that selectively attacks the peripheral cholinergic nerve endings. It is produced by Gram -positive, endospore-forming strict anaerobic bacteria, Clostridium botulinum. Since BoNT/A could be a biothreat agent, as well as a contaminator of food and water supplies, the development of sensitive assays for toxin detection and potent antitoxin for the treatment of intoxication is necessary. In this study, for the purpose of producing monoclonal antibodies (mAbs) that are capable of neutralizing Botulinum neurotoxin type A (BoNT/A), scFv (single-chain variable domain fragment) libraries from the rabbit antisera against BoNT/A was fused to a human IgG. The resulting recombinant scFvIgG antibody protein was expressed in stable cell lines and was purified using a protein A affinity chromatography. The efficacy of scFvIgG mAb was confirmed by ELISA and was evaluated for the neutralization of BoNT/A in vivo. Such an in vivo toxin neutralization assay was performed using mice. Although scFvIgG antibody proteins (10 ug) failed to fully protect the mice challenged with BoNT/A (100,000 $LD_{50}$), it significantly prolonged the survival time. These results suggest that scFvIgG mAb may be capable of neutralizing BoNT/A single-chain variable domain fragment.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.3
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• pp.280-287
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• 1997

This study was carried out to investigate agricultural status and soil fertility of Bonghwang-cheon watershed area which is located at Chodong-myeon, Milyang-si. The acreage of arable land was about 1,209ha, and the population was 3,706 people. Out of total arable land, paddy was 64.4%, upland 19.6% and orchard 16.0%. Chicken was the largest in the number of livestock and poultry, but pig seems to contribute most severly to water pollution. The geology in the lower stream was alluvium but andesite and derivated materials from it were occupied most upland areas. Tuff was scattered in the small area near by the alluvium of Nakdong river, and diorite and granodiorite were located at the low hill and mountains respectivately. The principal paddy soils in the area were Geumgog, Jisan and Imgog series etc of fine loamy on sloped valleys and fans. The Pyeongtaeg series, fine silty family of imperfectly drained on plain was irrigated by Chodong reservoir. The clayey Honam series on alluvium was distributed along the low hill while Gyuam series, coarse silty alluvial soil way close to Nakdong river. In chemical properties of soils, the vinyl house soils were higher in EC, and orchard soils in OM, $P_2O_5$, exchangeable canons than other soils.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.2
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• pp.210-224
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• 2003

Sulfate reduction is a microbiological process which occurs ubiquitously in anaerobic marine environment. Sulfate reducing bacteria play a significant role in anaerobic decomposition of organic matter and regeneration of inorganic nutrients which supports the primary production in the water column (i.e., benthic-pelagic coupling) and, in special case, could be responsible for the harmful algal bloom in the coastal marine environment. Summary of the sulfate reduction rates reported in various marine sedimentary environments revealed that supply of organic substrates and presence of various electron acceptors (i.e., $O_2$, NO$_{3}$$^{[-10]}$ , Fe(III) and Mn(IV), etc.) for other aerobic and anaerobic respiration directly affect the sulfate reduction rate and relative significance of sulfate reduction in organic matter mineralization. Significance of temperature, macrophytes and bioturbation is discussed as factors controlling supply of organic substrates and distribution of electron acceptors. Finally, we suggest studies on the anaerobic microbiological processes associated with biogeochemical element cycles in the coastal environments of Korea where massive operation of organic enriched fish cage farm, frequent occurrence of toxic algal bloom and hypoxia and conservation of tidal flat are of major environmental issues.

• Economic and Environmental Geology
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• v.54 no.2
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• pp.199-212
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• 2021

The Sebyeonggwan Hall (National Treasure No. 305) is located on the Naval Headquarter of Three Provinces in Tongyeong, and it has partly undergone with several rebuilding, remodeling, repairing and restorations since it's the first establishment in Joseon Dynasty (AD 1605) of ancient Korea. This study focuses on 50 foundation stones that comprise the Sebyeonggwan. These stones are made of six rock types and currently have various shapes of the surface damages. As the foundation stones, the dominant rock type was dacitic lapilli tuffs, and provenance-based interpretation was performed to supply alternative stones for conservation. Most of the provenance rocks for foundation stones showed highly homogeneity with their corresponding stones of petrography, mineralogy and magnetic susceptibility. According to surface deterioration assessments, the most serious damages of the stones were blistering and scaling. The deterioration mechanism was identified through the analysis of inorganic contaminants, and the primary reason is considered salt weathering caused by sea breeze and other combined circumstances. Based on the mechanical durability of the stones, there was no foundation stone that required the replacement of its members attributed to the degradation of the rock properties, but conservation treatment is considered necessary to delay superficial damage. The foundation stones are characterized by a combined outcome of multiple petrological factors that caused physical damage to surfaces and internal defects. Therefore, it's required to diagnosis and monitoring the Sebyeonggwan regularly for long-term preservation.

• Journal of the Mineralogical Society of Korea
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• v.22 no.3
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• pp.177-189
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• 2009

Iron (oxyhydr)oxides commonly form as secondary minerals of high reactivity and large surface area resulting from alteration and weathering of primary minerals, and they are efficient sorbents for inorganic and organic contaminants. Accordingly, they have a great potential in industrial applications and are also of substantial interest in environmental sciences. Goethite (${\alpha}$-FeOOH) is one of the most ubiquitous and stable forms of iron (oxyhydr)oxides in terrestrial soils, sediments, and ore deposits, as well as a common weathering product in rocks of all types. This study focused on adsorption reaction as a main mechanism in scavenging arsenic using goethite. Goethite was synthesized in the laboratory to get high purity, and a variety of mineralogical and physicochemical features of goethite were measured and related to adsorption characteristics of arsenic. To compare differences in adsorption reactions between arsenic species, in addition, a variety of experiments to acquire adsorption isotherm, adsorption edges, and adsorption kinetics were accomplished. The point of zero charge (PZC) of the laboratory-synthesized goethite was measured to be 7.6, which value seems to be relatively higher, compared to those of other iron (oxyhydr)oxides. Its specific surface area appeared to be $29.2\;m^2/g$ and it is relatively smaller than those of other (oxyhydr)oxides. As a result, it was speculated that goethite shows a smaller adsorption capacity. It is likely that the affinity of goethite is much more larger for As(III) (arsenite) than for As(V) (arsenate), because As(III) was observed to be much more adsorbed on goethite than As(V) in equivalent pH conditions. When the adsorption of each arsenic species onto goethite was characterized in various of pH, the adsorption of As(III) was largest in neutral pH range (7.0~9.0) and decreased in both acidic and alkaline pH conditions. In the case of As(V), the adsorption appeared to be highest in the lowest pH condition, and then decreased with an increase of pH. This peculiarity of arsenic adsorption onto goethite might be caused by macroscopic electrostatic interactions due to variation in chemical speciation of arsenic and surface charge of goethite, and also it is significantly affected by change in pH. Parabolic diffusion model was adequate to effectively evaluate arsenic adsorption on goethite, and the regression results show that the kinetic constant of As(V) is larger than that of As(III).

• Korean Journal of Environmental Agriculture
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• v.28 no.2
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• pp.194-201
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• 2009

To evaluate the exposure of non-point source pesticide pollution in agricultural watershed and to investigate pesticide distribution and runoff from agricultural land, paddy field, upland and orchard, this experiment was carry out during crop growing seasons. The pesticide were detected twenty pesticides fungicide 4, insecticide 10, herbicide 6) in water of Neungchon agricultural watershed and detection concentrations were range 0.008${\sim}$7.59 ppb. Most of the detection pesticides were using pesticides to rice paddy fields to control fungi, insects, weeds. During the crop cultivation, the pesticide were detected total thirty pesticides by pepper field soil 6, orchard soil 4, sesame field soil 3 and rice paddy field soil 5, and pesticide concentrations were range 0.001${\sim}$0.109 ppm. Especially the herbicides were detected mainly in May and June in the stream water. The pesticide were detected thirty pesticides by fungicide 2, insecticide 6, herbicide 5 in water of Jungam Koseong agricultural watershed and detection concentrations were range 0.01${\sim}$7.21 ppb. In regard to the detected pesticides, the concentration of individual pesticides measured in surface water of the study areas never exceeded guidelines for agriculture chemicals concerning water quality-effluent from paddy fields in Japan (Katayama, 2003). Runoff rate of pesticides was range 0.07${\sim}$3.06 % from Kongju agricultural land to watershed after applied pesticides.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.4
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• pp.642-648
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• 2012

To evaluate the water quality in Juksancheon constructed wetlands for treating non-point source pollution, the removal rates of nutrients in water and the total amounts of T-N and T-P uptakes by water plants were investigated. Chemical characteristics of T-N and T-P in sediment were investigated. The concentrations of BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand), SS (Suspended Solids), T-N and T-P in inflow were 0.07~1.47, 0.60~2.65, 0.50~4.60, 1.38~6.26 and $0.08{\sim}0.32mg\;L^{-1}$, respectively. The removal rates of BOD, COD, SS, T-N, and T-P were -10, 51, 66, -3 and 5%, respectively. The maximum amount of T-N uptake by water plants in August was $368.7mg\;plant^{-1}$ in the $2^{nd}$ treatment stage by Nymphoides peltata, $1314.6mg\;plant^{-1}$ in the $3^{rd}$ treatment stage by Iris pseudacorus, $1160.4mg\;plant^{-1}$ in the $4^{th}$ treatment stage by Nymphaea tetragona GEORGI, respectively. The maximum amount of T-P uptake by water plants in August was $121.7mg\;plant^{-1}$ by Nymphoides peltata in the $2^{nd}$ treatment stage, $268.7mg\;plant^{-1}$ by Iris pseudacorus in the $3^{rd}$ treatment stage and $212.0mg\;plant^{-1}$ by Nymphaea tetragona GEORGI in the $4^{th}$ treatment stage, respectively. Organic matter contents in sediments were not different. Contents of T-N and T-P in sediments were higher in spring. Microbial biomass C:N:P ratios in sediments in spring, summer, autumn and winter were 117~140:1~4:1, 86~126:5~6:1, 68~101:2~6:1 and 47~138:2~4:1, respectively. We could conclude that Juksancheon constructed wetlands show high removal efficiencies of COD and SS. However, improvements of management in winter season should be considered to improve the removal efficiencies of pollutants.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.400-407
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• 2011

To evaluate the aquatic ecological characteristics in Sinpyongcheon constructed wetlands for treating nonpoint source pollution, the removal rates of nutrients in water, the total amounts of T-N and T-P uptakes by water plants, and chemical characteristics of T-N and T-P in sediment were investigated. The concentrations of BOD, COD, SS, T-N and T-P in inflow were 0.07~1.47, 0.60~2.65, 0.50~4.60, 1.38~6.26 and $0.08{\sim}0.32mg\;L^{-1}$, respectively. The removal rates of BOD, COD, SS, T-N, and T-P were 14%, 6%, 18%, 24%, and 10%, respectively. The maximum amount of T-N uptake by water plants in August was $813mg\;plant^{-1}$ for Phragmites communis TRIV in $2^{nd}$ bed, $1,172mg\;plant^{-1}$ for Typha orientalis PRESL in $3^{rd}$ bed, respectively. The maximum amount of T-P uptake by water plants in August was $247mg\;plant^{-1}$ for Phragmites communis TRIV in $2^{nd}$ bed, $359mg\;plant^{-1}$ for Typha orientalis PRESL in $3^{rd}$ bed, respectively. Organic matter, T-N, and T-P contents in sediments were high in the order of $1^{st}$ bed > $2^{nd}$ bed > $3^{rd}$ bed. Microbial biomass C/N/P ratios in sediments in $1^{st}$, $2^{nd}$, and $3^{rd}$ were 78~110/3~6/1, 73~204/1~6/1, and 106~169/1~6/1, respectively.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.3
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• pp.348-356
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• 2007

Statistical and probabilistic method was used in the analysis of data, which is the most effective one in describing the various natures, and the methodology relating the results with the design was developed. Influents and effluents of three treatment plants were analyzed and the focus was made on BOD, COD, SS, IN, TP The fluctuations of influent such as BOD, COD, SS were extremely large and their standard deviations(st.dev) were more than 10 mg/L. but those of TN, TP were small; the st.dev was 6.6 mg/L for TN, 0.6 mg/L for TP, respectively. But, effluent concentration showed consistent pattern regardless of the influent fluctuations, the st.dev was ranged between 0.28 and 4.48 mg/L. Effluent distributional characteristics were as follows; BOD, COD were distributed normally, but SS, TN, and TP, log-normally; unsymmetric and skewed to the right. The coefficient of reliability(COR) based on the results of statistics of data was introduced to evaluate the process performance an4 to reflect the process performance to the process design. The coefficient of reliability relates the design value(the goal) with the standards and it can be used in operating treatment facilities under a certain reliability level and/or in evaluating the reliability of the treatment facilities on operation. Each treated water quality of effluent showed the half of water quality standards in the level of 50% percentile and all treatment plant was achieved 100% probability of water quality standards. It was concluded that the variability of the process performance should be reflected to the design procedure and the standards through the analysis based on the statistics and the probability.