• Korean Journal of Plant Tissue Culture
• /
• v.27 no.3
• /
• pp.185-189
• /
• 2000

For the micropropagation, node explants of cassava were cultured in liquid MS medium with various concentrations of cytokinins on a rotary shaker (100 rpm) for 2 weeks. The adventitious roots and shoots from the explants were differentiated more efficiently in liquid medium than in solid. But root formation was not inhibited in medium with BAP and kinetin at low concentration (＞0.05 mg 1/sup -1/), while in medium added with BAP and zeatin at high level (＜0.25 mg 1/sup -1/), it was inhibited by callus forming on cut end of the cuttings. However, all of plantlets grown in liquid medium for more than 2 weeks showed symptoms of hyperhydricity. The plantlets grown in liquid medium were transferred into culture bottles filled with fine sand or artificial soil (pitmoss：perlite：vermiculite, 1：1：1 v/v) wetted with half strength of Knop's solution. After transplanted to culture bottles, some of vitriscent leaves were defoliated and new leaves were normally formed from shoot apex. Most of plantlets (＞95%) were hardened-off successfully only in culture bottles with fine sand, and grew into 3-5 cm seedlings possessing 4-6 nodes after 4 weeks. Thus, the mass propagation of cassava on medium containing cytokinin could be established based on the suspension culture using node explants.

• Journal of Life Science
• /
• v.23 no.2
• /
• pp.182-189
• /
• 2013

Halophytes are unique land plants that are capable of thriving in a high-salt environment. They are attracting public attention due to their ability to synthesize bioactive substances such as UV protectants or antioxidizing agents. To achieve unaffected growth under high salinity, halophytes may take advantage of the activities of cell growth factors such as expansins. Expansins are well-known cell wall proteins that are responsible for cell enlargement. They loosen cell walls, thereby contributing to actual plant growth. This study aimed to identify positive roles of expansins in the growth of halophytes. Three expansin cDNA clones were isolated from seedlings of Suaeda japonica. Comparing the deduced amino acid sequences of the expansin genes of S. japonica with those of other plant species suggested that the cDNA clones isolated from S. japonica belong to the EXPA (${\alpha}$-expansin) gene family. A phylogenetic tree based on the deduced amino acid sequences revealed that the expansins of S. japonica share a close evolutionary relationship with those of strawberry (Fragaria ananassa) and jujube (Ziziphus jujuba), both of which are woody dicots. SjEXPAs did not show any remarkable change in the gene expression level in different NaCl concentrations, providing a clue to the unaffected seedling growth of S. japonica in a high-salt environment. In conclusion, the present study presents the first report of expansin genes from halophytes and suggests a putative role for these genes in plant growth under high salinity.

• FLOWER RESEARCH JOURNAL
• /
• v.18 no.2
• /
• pp.110-116
• /
• 2010

This text was analyzed and investigated the vegetation and floristic composition by ordination and classification of phytosociological method, to evaluate the species composition and community structure of Cymbidium goeringii group in Korean islets. In habitat of 33 plots, the mean altitude was 65.9m, the direction was the southeast slope, the mean slope was 7.9%. The coverage of Cymbidium goeringii was 4.5%. The appearing plants with the Cymbidium goeringii was the total 102 taxa, and it was the kind of trees 68 taxa (66.7%), herbs 34 taxa (33.3%), evergreen plants 36 taxa (35.3%) and deciduous plants 66 taxa (64.7 %) respectively. The frequency of appearing plant was the highest in the Eurya japonica (48.5%), followed by Pinus thunbergii (45.5%), Smilax china (36.4%), Carex lanceolata (33.3%), Hedera rhombea (33.3%), Machilus thunbergii (30.0%), Styrax japonicus (30.3%) and Pinus densiflora (27.3%), respectively. The vegetation of tree layer in Cymbidium goeringii group was classified into Pinus thunbergii community, Pinus densiflora community, Castanopsis sieboldii community and Quercus variabilis community. Pinus densiflora community showed a strong combination with Cymbidium goeringii group in Korean islets. Pinus thunbergii community among communities was combined with Castanopsis sieboldii community, and Pinus densiflora community and Quercus variabilis community were combined.

• Journal of agriculture & life science
• /
• v.46 no.4
• /
• pp.57-68
• /
• 2012

This study was conducted to provide basic data for the effective conservation and management in the black pine(Pinus thunbergii) stand which is located in the eastern coast of Gyeongsangbuk-do where the fragmentation of vegetation has been caused by the exploitation and the increase of tourists, and installed the seventy study sites($20{\times}20m$) in the dominant black pine stand. The black pine stand was classified into three groups(P. thunbergii - P. densiflora community, P. thunbergii - Robinia pseudoacacia community, P. thunbergii - P. densiflora-Celtis sinensis community) by a cluster analysis. As a result of Multi-Response Permutation Procedures test, there is significance among the communities. Pinus densiflora, Callicarpa japonica, Juniperus rigida, Rhododendron yedoense for. poukhanense etc. nineteen species were significant by indicator species analysis. The population structure of black pine stand showed that the class of diameter 12 to 26 cm was dominance, which had the inverse hump-shape pattern. Species diversity index(H') of investigated ranged from $1.033{\pm}0.234$ to $1.629{\pm}0.226$ in the woody layer group and from $2.448{\pm}0.457$ to $2.545{\pm}0.318$ $2.174{\pm}0.333$ in the herb layer group.

• Korean Journal of Environment and Ecology
• /
• v.33 no.5
• /
• pp.565-577
• /
• 2019

The purpose of this study was to understand the factors influencing the decline and the patterns of changes in Abies nephrolepis populations on Mt. Seorak. We installed permanent quadrats in the areas of the Gwittagicheongbong (peak), Gwanmoneungseon (ridge), and the Seorak Falls and have monitored the community structure, population dynamics, radial growth, and vitality in the quadrats since 2009. Excluding the Seorak Falls, the three research sites showed a three-layer structure in which the low-tree layer forms the canopy. Major tree species were Khingan fir, Korean arborvitae, Mongolian oak, Erman's birch, and Korean maple. The significance of Khingan fir in Seorak Falls decreased from 45.3% in 2009 to 36.8% in 2018. The number of shoots ($DBH{\geq}5cm$) was highest at 1,800 individuals/ha and 1,700 individuals/ha at the Gwittagicheongbong 2 and the Gwanmoneungseon, respectively. The mortality rates over the past 10 years were very high, at 38.3% and 35.3%, respectively, in the Gwittagicheongbong 1 and Seorak Falls. The most stable inverse J-shaped distribution in the Gwittagicheongbong 1 area was shown in the size-frequency distribution of the Khingan fir populations. The average annual ring growth of the Khingan fir was 0.96 ~ 1.73 mm/year, and the ring growth tended to decrease in the areas of Gwittagicheongbong 1, Gwanmoneungseon, and Seorak Falls, where the vitality was low. If the monitoring process continues, it will be possible to obtain basic data for the conservation and management of subalpine vegetation.

• Journal of Korean Society of Forest Science
• /
• v.110 no.4
• /
• pp.577-589
• /
• 2021

Soil seed bank community contributes to the long-term conservation of plant diversity and vegetation dynamics, and their decreasing diversity and density with soil depth provide critical perspectives (deterministic and stochastic) for understanding the community disassembly process. We analyzed changes in species composition and diversity and structuring patterns by soil layer (top and bottom), including surface vegetation, in Gwangneung Forest, a mature forest with a vegetation climate in the temperate central part of the Korean Peninsula. From two layers of soil collected with a vertical difference of 10 cm, 934 specimens of 27 families, 40 genera, 44 species, three varieties, and 47 taxa, germinated. Although species diversity and germination density decreased in most comparative characteristics, including growth type, there was no statistical significance due to large deviations. Within-group variability of species composition was similar in the upper and lower soils, as was the decline pattern in co-occurred species (ζ-diversity) and change in species retention probability. The structuring process of the community composition in the two soil layers was fitted with an exponential correlation rather than a power function, demonstrating the dominance of the stochastic process. The pattern in diversity and species turnover according to soil depth in Gwangneung Forest was discovered to be structured by stochastic random events, such as seed vertical movement rather than interaction with trait characteristics.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.21 no.3
• /
• pp.196-207
• /
• 2019

This study was carried out to investigate the effects of C. japonica, D. morbifera, D. macropodum, I. anisatum, Q. glauca and R. indica To investigate the photosynthetic ability, chlorophyll content, chlorophyll fluorescence analysis, and physiological environmental. The photosynthetic rate, cancer respiration rate, stomatal conductance, and rate of evaporation tended to decrease as a result of drying stress in the no-water condition for 28 days. I. anisatum, Q. glauca and R. indica showed a low rate of less than 40% until 28 days of no-treatment. The total chlorophyll contents were decreased in the order of D. macropodum> D. morbifera> C. japonica> Q. glauca> M. thunbergii> R. indica> I. anisatum. Chlorophyll fluorescence analysis showed that there was no change in the qP, but after 28 days no $Fv/F_m$, $F_o$, $R_{fd}$, $NPQ_{_-LSS}$ can be a useful indicator for quantitative estimation within a short period of time with a marked reduction rate of PSII quantum yield ${\Phi}PSII$ in the rectified state by continuous light during the nominal adaptation period. In the case of I. anisatum, Q. glauca and R. indica If water management can be carried out at intervals, it may be possible to plant trees in trees and landscape trees.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.66-66
• /
• 2019

본 연구조사의 대상지인 백두대간은 시점인 백두산에서 종점인 지리산까지 이어지는 한반도 전체를 관통하는 핵심 생태축이며, 남북한을 이어주는 산맥으로 인정되고 있다. 그 중 속리산 권역내 늘재하늘재(56.3km) 구간을 2018년 4월에서 10월까지 계절별로 식물의 고도별 수직분포, 식물상을 조사하여 한반도 식물의 기초 자료를 조사하고자 한다. 조사구간의 고도는 최저 310m에서 최고1,052m(평균 726m)로 전형적인 온대낙엽활엽수-침엽수 혼효림으로 구분된다. 주요 조사지인 300m1100m 고도에서 발견된 식물은 85과 227속 358종 4아종 37변종 5품종 총 404종이 출현하였으며 이중 한국특산식물은 노각나무, 자란초, 처녀치마 등 15과 19속 19종 2변종 총 21분류군, 희귀식물은 개병풍, 모데미풀 등 12과 19속 22종 총 22분류군, 기후변화 취약식물은 특산식물 9종, 남방계 1종, 북방계 16종으로 15과 20속 25종 총 25분류군으로 북방계 식물이 대다수 등장하였다. 고도별 분포를 보면 접속구간 300m 제외한 400m 107종, 500m 306종, 600m 137종, 700m 209종, 800m 190종, 900m 92종으로 종수-고도간 유의관계는 낮았으나, 생활형으로 구분한 목본(MM, M, N) 및 초본(CH, HH, H, G, Th, E)간 종의 구성비에서는 유의한 상관관계를 보였다. 400m에서 목본의 구성비는 40%였으나 고도에 따라 증가해 900m에서는 61%에 달했으며, 초본의 구성비는 60%에서 40%로 낮아졌다. 해당 구간의 이러한 경향을 봤을 때 마루금의 평균 해발고도 중심으로 식물이 주로 발견된 경향이 있으나 구성비의 변화를 봤을 때 700m를 중심으로 변화가 일어나고 있었으며 이는 각 정상부, 접속부의 물리적 환경의 차이로 이해된다.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.23 no.1
• /
• pp.34-45
• /
• 2021

Weather is one of the important factors in the agricultural industry as it affects the price, production, and quality of crops. Upland crops are directly exposed to the natural environment because they are mainly grown in mountainous areas. Therefore, it is necessary to provide accurate weather for upland crops. This study examined the effectiveness of 12 forest soil factors to interpolate the weather in mountainous areas. The daily temperature and precipitation were collected by the Korea Meteorological Administration between January 2009 and December 2018. The Generalized Additive Model (GAM), Kriging, and Random Forest (RF) were considered to interpolate. For evaluating the interpolation performance, automatic weather stations were used as training data and automated synoptic observing systems were used as test data for cross-validation. Unfortunately, the forest soil factors were not significant to interpolate the weather in the mountainous areas. GAM with only geography aspects showed that it can interpolate well in terms of root mean squared error and mean absolute error. The significance of the factors was tested at the 5% significance level in GAM, and the climate zone code (CLZN_CD) and soil water code B (SIBFLR_LAR) were identified as relatively important factors. It has shown that CLZN_CD could help to interpolate the daily average and minimum daily temperature for upland crops.

• Journal of Environmental Impact Assessment
• /
• v.31 no.6
• /
• pp.409-422
• /
• 2022

Riverine wetlands are an important element of the river ecosystem and account for approximately 38% of the inland wetlands surveyed so far. The Shincheon Wetland located in Mangyeong River is also a channel wetland as the flow rate is slowed by the constructed weirs, leading to sediment accumulation. To identify the conservation value and ecological characteristics of Shincheon Wetland, its vegetation and plant diversity were identified using a phytosociological method, and a total of 45 vegetation-related datasets were collected. Overall, 24 plant communities, comprising a total of 153 taxa (49 families, 117 genera, 146 species, 2 subspecies, 5 varieties) were identified. The plant with the highest appearance rate in the communities was Humulus japonicus Siebold & Zucc. In addition, annual herb species, including Rumex crispus L., Bromusjaponicus Thunb., Erigeron annuus (L.) Pers., and Artemisia indica Willd. were frequently observed to be growing in the secondary grassland. Naturalized plants were surveyed in the 38 taxa; the urbanization index was 10.3% and the naturalized index was 24.8%. Plant communities were largely classified into submerged vegetation, floating and floating-leaved vegetation, annual and biennial vegetation, perennial herb vegetation, and woody vegetation. The distribution of plant communities reflecting various habitats, including the lentic and lotic zone maintaining a constant water depth, littoral zone experiencing intermittent water level fluctuations, and dry floodplain environment was also confirmed. Overall, plant community development plays an important role in the habitat for wild animals; therefore, it is expected to positively impact biodiversity enhancement.