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Characterization of Edwardsiella tarda specific phage isolated from fish farms on west coast of Korea (서해안 양식장에서 분리한 Edwardsiella tarda의 특이 bacteriophage 특성)

  • Lee, Chan-Heun;Heo, Yong-Ju;Baek, Min-Suk;Lee, Jung-Uen;Kang, Ja-Young;Han, Mi-Jung;Kyoung, Se-Bong;Choi, Sang-Hoon
    • Journal of fish pathology
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    • v.24 no.2
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    • pp.85-93
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    • 2011
  • Edwardsiella tarda is a broadly infectious agent against freshwater and seawater fishes. In the present study, E. tarda specific phage was isolated from fish farms on the west coast of Korea, and the effect of environmental factors such as pH and water temperature on the phage activity was investigated. As a fish model, Nile tilapia (Oreochromis niloticus) was used and the interaction between E. tarda and phage was investigated. The phage activity, in pH test, appeared even higher in seawater than freshwater and was evenly constant up to $50^{\circ}C$. The phage and E. tarda were inoculated in tilapia and the phage activity and E. tarda viability were checked on the time intervals. As a result, the number of E. tarda in the group of E. tarda plus phage was constantly reduced to 24 hr post-inoculation compared to the control group without phage, whereas the phage activity was slightly increased in the experiment group. The results suggest that it might be possible to use phage to control the fish disease caused by E. tarda.

Flexural and Shear Behavior of Reinforced Dual Concrete Beam (철근 이중 콘크리트 보의 휨 및 전단 거동)

  • Park Tae-Hyo;Park Jae-Min;Kim Hee-Dae
    • Journal of the Korea Concrete Institute
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    • v.17 no.3 s.87
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    • pp.401-409
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    • 2005
  • In this study, reinforced dual concrete beam (RDC beam) composed of steel fiber reinforced concrete (SFRC) in the tension part and normal strength concrete (NSC) in the compression and remaining part is proposed. It is the epochal structural system that improves the overall structural performances of beam by partially superseding the steel fiber reinforced concrete in the lower tension part of conventional reinforced concrete beam (RC beam). Flexural and shear tests are performed to prove the structural excellence of RDC beam in comparison with RC beam. An analytical method is proposed to understand the flexrual behavior and is compared to experimental results. And for shear behavior, experimental results are compared to empirical equations predicting the ultimate shear strength of full-depth fiber reinforced concrete beam to examine the behavior of RDC beam under shear. From this studies, it is proved that RDC beam has more superior structural performance than RC beam, and the analytical method for flexural behavior agrees well with experimental results, and the partial-depth fiber reinforcements have no noticeable effect on ultimate shear strength but it is considerably effective to control and prevent evolutions of crack.

Cracking Behavior and Flexural Performance of RC Beam with Strain Hardening Cement Composite and High-Strength Reinforcing Bar (고강도 철근과 변형경화형 시멘트복합체를 사용한 보의 균열거동 및 휨 성능)

  • Jang, Seok-Joon;Kang, Su-Won;Yun, Hyun-Do
    • Journal of the Korea Concrete Institute
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    • v.27 no.1
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    • pp.37-44
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    • 2015
  • This paper describes the effect of strain hardening cement composite (SHCC) material on structure performance of reinforced concrete (RC) beams with high-strength reinforcing bar. Also, this paper explores the structure application of SHCC in order to mitigation cracking damage and improve the ductility of flexural RC members. The prediction model for flexural strength of doubly reinforced SHCC beams are investigated in this study. To achieve the these objectives, a total of 6 rectangular beam specimens were tested under four point monotonic loading condition. The main parameters included the types of cement composite and reinforcing bar. Test results indicated that reinforced beam specimens with SHCC material were improved the structure performances and damage characteristics. Specifically, replacement of conventional high-strength concrete with SHCC materials has the potential of high-strength steel bar as flexural reinforcement on RC members. It is remarkable that suggested method of reinforced SHCC beams with high-strength reinforcing bar could be used usefully to the structure design.

Pre-Packing, Early Fixation, and Multi-Layer Density Analysis in Analytic Placement for FPGAs (FPGA를 위한 분석적 배치에서 사전 패킹, 조기 배치 고정 및 밀도 분석 다층화)

  • Kim, Kyosun
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.10
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    • pp.96-106
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    • 2014
  • Previous academic research on FPGA tools has relied on simple imaginary models for the targeting architecture. As the first step to overcome such restriction, the issues on analytic placement and legalization which are applied to commercial FPGAs have been brought up, and several techniques to remedy them are presented, and evaluated. First of all, the center of gravity of the placed cells may be far displaced from the center of the chip during analytic placement. A function is proposed to be added to the objective function for minimizing this displacement. And then, the density map is expanded into multiple layers to accurately calculate the density distribution for each of the cell types. Early fixation is also proposed for the memory blocks which can be placed at limited sites in small numbers. Since two flip-flops share control pins in a slice, a compatibility constraint is introduced during legalization. Pre-packing compatible flip-flops is proposed as a proactive step. The proposed techniques are implemented on the K-FPGA fabric evaluation framework in which commercial architectures can be precisely modeled, and modified for enhancement, and validated on twelve industrial strength examples. The placement results show that the proposed techniques have reduced the wire length by 22%, and the slice usage by 5% on average. This research is expected to be a development basis of the optimization CAD tools for new as well as the state-of-the-art FPGA architectures.

Numerical Study on the Performance Assessment for Defrost and De-Icing Modes (승용차의 제상 및 성에 제거 성능 평가를 위한 수치해석적 연구)

  • Kim, Yoon-Kee;Yang, Jang-Sik;Kim, Kyung-Chun;Ji, Ho-Seong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.2
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    • pp.161-168
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    • 2011
  • The heating, ventilating, air conditioning (HVAC) system is a very important part of an automotive vehicle: it controls the microclimate inside the passenger's compartment and removes the frost or mist that is produced in cold/rainy weather. In this study, the numerical analysis of the defrost duct in an HVAC system and the de-icing pattern is carried out using commercial CFX-code. The mass flow distribution and flow structure at the outlet of the defrost duct satisfied the duct design specification. For analyzing the de-icing pattern, additional grid generation of solid domain of ice and glass is pre-defined for conductive heat transfer. The flow structure near the windshield, streakline, and temperature fields clearly indicate that the de-icing capacity of the given defrost duct configuration is excellent and that it can be operated in a stable manner. In this paper, the unsteady changes in temperature, water volume fraction, and static enthalpy at four monitoring points are discussed.

Development of Oriental Melon Harvesting Robot in Greenhouse Cultivation (시설재배 참외 수확 로봇 개발)

  • Ha, Yu Shin;Kim, Tae Wook
    • Journal of Bio-Environment Control
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    • v.23 no.2
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    • pp.123-130
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    • 2014
  • Oriental melon (Cucumis melo var. makuwa) should be cultivated on the soil and be harvested. It is difficult to find because it is covered with leaves, and furthermore, it is very hard to grip it due to its climbing stems. This study developed and tested oriental melon harvesting robots such as an end-effector, manipulator and identification device. The end effector is divided into a gripper for harvest and a cutter for stems. In addition, it was designed to control the gripping and cutting forces so that the gripper could move four fingers at the same time and the cutter could move back and forth. The manipulator was designed to realize a 4-axis manipulator structure to combine orthogonal coordinate-type and shuttle-type manipulators with L-R type model to rotate based on the central axis. With regard to the identification device, oriental melon was identified using the primary identification global view camera device and secondary identification local view camera device and selected in the prediction of the sugar content or maturity. As a result of the performance test using this device, the average harvest time was 18.2 sec/ea, average pick-up rate was 91.4%, average damage rate was 8.2% and average sorting rate was 72.6%.

Effects of Platycodon Grandiflorum Including Platycodin D in IgE/Ag-Induced Type I Hypersensitivity (Platycodin D를 포함하는 도라지 추출물이 IgE/Ag 유도 제 1형 과민반응에 미치는 영향)

  • Park, Sae-Jin;Kim, Jong-Woo;Park, Sang-Jin;Kim, Tack-Joong
    • Journal of Life Science
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    • v.22 no.5
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    • pp.595-599
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    • 2012
  • Allergic response is associated with mast cells, through the release of arachidonic acid, proinflammatory cytokines, and histamine. We investigated the effect of Platycodon grandiflorum including platycodin D (PG-Platycodin D) on allergic responses in an animal model and on mast cell degranulation. PG-Platycodin D suppressed the release of ${\beta}$-hexosaminidase, a type of marker associated with degranulation. PG-Platycodin D efficiently inhibited the passive cutaneous anaphylaxis (PCA) reaction in ICR mice. In addition, molecular analysis demonstrated that PG-Platycodin D inhibited mRNA expression of both IL-3. These results suggest that PG-Platycodin D can inhibit mast cell degranulation through the inhibition of IL-3 mRNA expression, and that PG-Platycodin D may potentially serve as an anti-allergic agent.

Active Object Tracking System based on Stereo Vision (스테레오 비젼 기반의 능동형 물체 추적 시스템)

  • Ko, Jung-Hwan
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.4
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    • pp.159-166
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    • 2016
  • In this paper, an active object tracking system basing on the pan/tilt-embedded stereo camera system is suggested and implemented. In the proposed system, once the face area of a target is detected from the input stereo image by using a YCbCr color model and phase-type correlation scheme and then, using this data as well as the geometric information of the tracking system, the distance and 3D information of the target are effectively extracted in real-time. Basing on these extracted data the pan/tilted-embedded stereo camera system is adaptively controlled and as a result, the proposed system can track the target adaptively under the various circumstance of the target. From some experiments using 480 frames of the test input stereo image, it is analyzed that a standard variation between the measured and computed the estimated target's height and an error ratio between the measured and computed 3D coordinate values of the target is also kept to be very low value of 1.03 and 1.18% on average, respectively. From these good experimental results a possibility of implementing a new real-time intelligent stereo target tracking and surveillance system using the proposed scheme is finally suggested.

Ultrasound-Triggered Drug Release of Hydroxyapatite Coated Liposomes (하이드록시아파타이트 코팅 리포솜의 초음파에 의한 약물방출)

  • Cho, Sung Keun;Wee, Tae In;Ha, Jeung;Cho, Sun Hang;Han, Kun;Han, Hee Dong;Shin, Byung Cheol
    • Journal of the Korean Chemical Society
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    • v.57 no.4
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    • pp.493-498
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    • 2013
  • Liposomes, which can deliver payload at target site, have been studied as drug carrier. However, conventional liposomes have limitation for drug release at target site. Therefore, we developed hydroxyapatite (HA) coated ultrasound sensitive liposomes to increase drug release at target site and to enhance stability in blood stream. Control liposome was prepared using hydrogenated soy phosphatidylcholine (HSPC) and cholesterol, and then we assessed HA coating on the surface of control liposomes using calcium acetate, phosphoric acid, and 25% ammonium solution. Doxorubicin was used as a model drug. Size of HA coated liposomes was 120 nm and encapsulation efficiency of doxorubicin in liposomes was up to 95%. Size of HA coated liposomes are not changed in 30% serum solution, however, the control liposomes was 1.4 fold increased. After ultrasound triggered drug release from liposomes, intracellular efficiency of drug released from HA coated liposomes was 3 fold increased compared to control liposomes. In this study, we developed ultrasound sensitive liposomes to enhance drug release, which will be applied in controlled drug release at disease site.

Analysis of the thermal fluid flow between the gas torch and the steel plate for the application of the line heating (선상 가열을 위한 가스 토치와 강판 사이의 열유동 해석)

  • Jong-Hun Woo;Jong-Gye Shin
    • Journal of the Society of Naval Architects of Korea
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    • v.39 no.2
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    • pp.52-60
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    • 2002
  • Line heating is a forming process which makes the curved surface with the residual strain created by applying heat source of high temperature to steel plate. in order to control the residual strain, it is necessary to understand not only conductive heat transfer between heat source and steel plate, but also temperature distribution of steel plate. In this paper we attempted to analyze is temperature distribution of steel plate by simplifying a line heating process to collision-effusive flux of high temperature and high velocity, and conductive heat transfer phenomenon. To analyze this, combustion in the torch is simplified to collision effusive phenomenon before analyzing turbulent heat flux. The distribution of temperature field between the torch and steel plate is computed through turbulent heat flux analysis, and the convective heat transfer coefficient between effusive flux and steel plate is calculated using approximate empirical Nusselt formula. The velocity of heat flux into steel plate is computed using the temperature distribution and convective heat transfer coefficient, and temperature field in the steel plate is obtained through conductive heat transfer analysis in which the traction is induced by velocity of heat flux. In this study, Finite Element Method is used to accomplish turbulent heat flux analysis and conductive heat transfer analysis. FEA results are compared with empirical data to verify results.