Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Characterization of Edwardsiella tarda specific phage isolated from fish farms on west coast of Korea

서해안 양식장에서 분리한 Edwardsiella tarda의 특이 bacteriophage 특성

  • Lee, Chan-Heun (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Heo, Yong-Ju (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Baek, Min-Suk (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Lee, Jung-Uen (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Kang, Ja-Young (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Han, Mi-Jung (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Kyoung, Se-Bong (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, Kunsan National University)
  • 이찬흔 (군산대학교 해양과학대학 수산생명의학과) ;
  • 허용주 (군산대학교 해양과학대학 수산생명의학과) ;
  • 백민석 (군산대학교 해양과학대학 수산생명의학과) ;
  • 이정은 (군산대학교 해양과학대학 수산생명의학과) ;
  • 강자영 (군산대학교 해양과학대학 수산생명의학과) ;
  • 한미정 (군산대학교 해양과학대학 수산생명의학과) ;
  • 경서봉 (군산대학교 해양과학대학 수산생명의학과) ;
  • 최상훈 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2011.04.13
  • Accepted : 2011.08.13
  • Published : 2011.08.31
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Abstract

Edwardsiella tarda is a broadly infectious agent against freshwater and seawater fishes. In the present study, E. tarda specific phage was isolated from fish farms on the west coast of Korea, and the effect of environmental factors such as pH and water temperature on the phage activity was investigated. As a fish model, Nile tilapia (Oreochromis niloticus) was used and the interaction between E. tarda and phage was investigated. The phage activity, in pH test, appeared even higher in seawater than freshwater and was evenly constant up to $50^{\circ}C$. The phage and E. tarda were inoculated in tilapia and the phage activity and E. tarda viability were checked on the time intervals. As a result, the number of E. tarda in the group of E. tarda plus phage was constantly reduced to 24 hr post-inoculation compared to the control group without phage, whereas the phage activity was slightly increased in the experiment group. The results suggest that it might be possible to use phage to control the fish disease caused by E. tarda.

Edwardsiella tarda (E. tarda)는 담수 및 해수어에 광범위하게 감염을 일으키는 그람 음성 박테리아이다. 본 연구에서는 서해안 넙치 양식장에서 분리한 E. tarda에 대해 특이 용균을 보이는 파아지를 분리하여 파아지의 활성에 미치는 환경적 요인인 pH와 배양 온도의 영향 등을 조사하였다. 또한 어류모델로서는 담수어종인 Nile tilapia (Oreochromis niloticus)를 이용하였으며 E. tarda와 파아지와의 상호작용을 알아보았다. pH 안정성 실험에서는 담수보다 해수에서 높은 활성을 보였으며 온도 안정성 실험 결과 $50^{\circ}C$까지 일정한 활성을 보였다. Tilapia 감염 실험에서는 파아지와 E. tarda를 접종하였다. 그 결과 실험군은 대조군에 비해 24시간째까지 E. tarda수가 일정하게 감소하는 것을 확인하였으며 파아지는 처음 접종 양보다 약간 증폭됨을 알 수 있었다. 본 연구의 결과는 E. tarda에 의해 발생되는 질병을 특이 파아지를 이용하여 제어할 수 있음을 암시해준다.

Keywords

References

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