• Korean Journal of Optics and Photonics
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• v.24 no.2
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• pp.86-91
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• 2013

In this study, we have investigated the correlation of far-field detection sensitivity of surface plasmon resonance (SPR) biosensors with optical signatures associated with the near-field overlap of biomolecules. The results confirm a direct relation between the far-field and near-field parameters, particularly for optical signatures defined in terms of lateral electric field components that are tangential to the interface and thus continuous across the interface. The overall correlation between near-field optical signatures and far-field resonance shift exceeded 97%. The results can be highly useful to evaluate detection sensitivity of SPR biosensors that take advantage of complex structures for localization of surface waves.

• Korean Chemical Engineering Research
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• v.49 no.2
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• pp.224-229
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• 2011

In this study, we have developed shear horizontal(SH) surface acoustic wave(SAW) sensors for the detection of immunoglobulin G(IgG) on the gold coated delay line of SH-SAW devices. As the result of the experiment, we could uniformly immobilize anti-MIgG(mouse IgG) conjugate on the surface of gold. When displaying results of immobilization on the surface of gold using G-anti MIgG conjugate and blocking buffer in frequency shift, G-anti MIgG conjugate showed frequency shift of 75.1 kHz in the initial frequency, and blocking buffer showed frequency shift of 215.7 kHz. When various concentrations of MIgG was added in 100MHz type sensor, the sensor showed 46.3, 127.45, 161.21 and 262.39 kHz frequency shift at 25, 50, 75 and 100 ${\mu}g$ MIgG concentration, respectively.

• Proceedings of the Korea Information Processing Society Conference
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• 2012.11a
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• pp.1162-1165
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• 2012

어미로부터 생후 21일령(또는 28일령)에 젖을 때는 어린 자돈들은 면역력이 약하여 통상 폐사율이 30~40%까지 치솟는 등 자돈 관리가 국내 양돈 농가의 가장 큰 문제 중 하나로 인식되고 있다. 본 논문에서는 이러한 양돈 농가의 문제를 해결하기 위하여 자돈사(새끼돼지 축사)에 카메라와 마이크를 설치하고 획득된 영상과 소리 정보를 이용하여 자돈들을 모니터링하는 시스템을 제안한다. 제안된 시스템은 실시간으로 유입되는 영상과 소리 스트림 데이터로부터 각각 움직임 벡터와 평균 피치 값을 추출하여 이미 설정된 정상 상황의 임계치 값을 넘는 순간부터를 불특정 이상 상황이라 판단한다. 실제, 경상남도 함양군의 한 돼지 농장에 A/V 센서 기반의 실험 환경을 구축하고 2012년 6월 한 달간의 이유자돈 돈사의 모니터링 데이터 셋을 취득하였고 전반기 15일간의 데이터 셋을 이용하여 자돈사 모니터링 시스템의 프로토타입을 설계 구현하였으며 후반기 15일간의 A/V 스트림 데이터로는 검증 실험을 수행하였다.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.223-225
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• 2013

어미로부터 생후 21일령 또는 28일령에 젖을 때는 어린 자돈들은 면역력이 약하여 통상 폐사율이 30~40%까지 치솟는 등 자돈 관리가 국내 양돈 농가의 가장 큰 문제 중 하나로 인식되고 있다. 본 논문에서는 이러한 양돈 농가의 문제를 해결하기 위하여 자돈사에 비디오 카메라를 설치하고 획득된 영상 정보를 이용하여 자돈들을 관리하는 시스템을 제안한다. 특히 제안된 시스템은 실시간으로 유입되는 영상 스트림 데이터로부터 움직임 여부를 신속히 판단하고, 움직임이 없는 경우(수면 또는 휴식)에 바닥면적 중 자돈들이 차지하지 않은 부분의 면적을 추출하여 수면 또는 휴식 중 자돈들의 밀집 여부를 판단한다. 즉, 카메라를 이용하여 과도하게 밀집된 경우 온도를 올려주고 반대의 경우 온도를 낮춰주는 온도제어 시스템을 설계할 수 있다. 실제, 경상남도 함양군의 한 돼지 농장에 비디오 센서 기반의 실험 환경을 구축하고 자돈사 감시 데이터 셋을 취득하였고, 이를 이용하여 제안된 자돈사 관리 시스템의 프로토타입을 개발하였다.

• Journal of Sensor Science and Technology
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• v.7 no.4
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• pp.263-270
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• 1998

Surface Plasmon Resonance (SPR) sensor system, has rapid response and high sensitivity, can be applicable for detecting reaction times of many biospecific interactions. A SPR sensor system was constructed to detect the antigen-antibody reactions of salmonella and hIgG (human immunoglobulin G). Sensor chips made of gold thin film were used for detecting biological bindings of antigen and antibody reactions. The antigen and antibody reactions for salmonella and hIgG were carried out with various time intervals to observed characteristics of these reactions using SPR sensor system. The resonance angle shift changes were clearly observed at the time of salmonella or hIgG antibody injection into sample cell since each antibody was self-assembled on gold chip surface of the sensor. It was found that the antibodies of salmonella and hIgG reacted with its sensor chip surface in 10 minutes and 60 minutes respectively. And the antigens of both salmonella and hIgG were bound to its antibody within 1 minute.

• Journal of Food Hygiene and Safety
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• v.33 no.3
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• pp.193-199
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• 2018

In this study, PAT protein of genetically modified maize was prepared from the recombinant E. coli strain BL21 (DE3), and mice were immunized with the recombinant PAT protein. After cell fusion and cloning, two hybridoma cells (PATmAb-7 and PATmAb-12) were chosen since the monoclonal antibodies (Mabs) produced by them were confirmed to be specific to PAT protein in the indirect enzyme-linked immunsorbent assay (ELISA) and western blot tests. There were no cross-reactions of either Mabs to other GM proteins or to the extracts of non-GM maize. The ELISA based on the PATmAb-7 can sensitively detect 0.3 ng/g PAT protein in corn. These results indicate that the developed Mabs can be used as bio-receptors in the development of immunosensors and biosensors for the rapid and simple detection of GM corn adulterated in foods.

• Journal of Sensor Science and Technology
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• v.23 no.4
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• pp.278-283
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• 2014

In this study, we propose an immunosensor using zinc oxide nanorods (NRs) inside PDMS channel for detecting the influenza A virus subtype H7N9. ZnO with high isoelectric point (IEP, ~9.5) makes it suitable for immobilizing proteins with low IEP. In this proposed H7N9 immunosensor structure ZnO NRs were grown on the PDMS channel inner surface to immobilize H7N9 capture antibody. A sandwich enzyme-linked immunosorbent assay (ELISA) method with was used 3,3',5,5' tetramethylbenzidine (TMB) for detecting H7N9 influenza virus. The immunosensor was evaluated by amperometry at various H7N9 influenza antigen concentrations (1 pg/ml - 1 ng/ml). The redox peak voltage and current were measured by amperometry with ZnO NWs and without ZnO NWs inside PDMS channel. The measurement results of the H7N9 immunosensor showed that oxidation peak current of TMB at 0.25 V logarithmically increased from 2.3 to 3.8 uA as the H7N9 influenza antigen concentration changed from 1 pg/ml to 1 ng/ml. And then we demonstrated that ZnO NRs inside PDMS channel can improve the sensitivity of immunosensor to compare non-ZnO NRs inside PDMS channel.

• Proceedings of the KIEE Conference
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• 2011.07a
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• pp.1690-1691
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• 2011

본 논문에서는 H1N1 인플루엔자 A 바이러스(influenza A H1N1 virus) 검출을 위한 산화아연 나노구조(zinc oxide nano structure) 기반의 전기화학적 면역센서를 제작하고 그 특성을 분석하였다. H1N1 인플루엔자 A 바이러스는 빠른 전파 속도 때문에 정확하고 빠른 검출이 필요하다. 먼저, 2 $mm^2$의 표면적을 갖는 패턴된 금 전극 위에 열수방식(hydrothermal method)으로 성장시킨 산화아연 나노구조가 선택적으로 형성되도록 리프트-오프(lift-off) 방법을 사용하였다. 0.01 M phosphate buffered saline(pH 7.4)에서 2 ${\mu}g$/mL 농도의 1차 항체를 정전기력에 의해 산화아연 나노구조에 고정화한 후, 10 pg/mL ~ 5ng/mL 농도의 H1N1 항원을 적용하여 포획 항체에 결합시키고 HRP(horseradish peroxidase) 효소가 결합된 검출 항체를 항원에 결합시키는 샌드위치 ELISA법을 이용하였다. HRP와 반응하는 TMB(3,3', 5,5'-tetramethylbenzidine)와 과산화수소가 포함된 acetate buffered 용액(pH 5)을 전해질로 사용하고 순환전압전류 측정법(cyclic voltammetry)으로 센서의 특성을 분석하였다. 측정된 순환전압전류그래프(cyclic voltammogram)에서 H1N1 항원 농도 10 pg/mL ~ 5 ng/mL의 응답 전류는 276.47 ${\pm}$ 21.72 nA (평균 ${\pm}$ 표준편차, n=4) ~ 478.89 ${\pm}$ 6.21 nA로 측정되었고, logarithmic하게 증가하는 응답 전류 특성을 보였다.

• Journal of Sensor Science and Technology
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• v.16 no.6
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• pp.441-448
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• 2007

In this study, the fiber-optic fluoro-immunosensor was designed to detect foodborne pathogens. The fabricated system is composed of the multimode optical fiber on which antibodies are immobilized. Then, a sandwich immunoassay is applied to the fabricated the fiber-optic fluoro-immunosensor. In the "sandwich" binding format, a primary or "capture" antibody is immobilized on the core surface of the multimode optical fiber and a secondary or named as "tracer" antibody is added to the bulk solution. A tracer is labeled FITC (fluorescein isothiocyanate; ${\lambda}ex$=492 nm, ${\lambda}em$= 520 nm). Different concentrations of antigens are tested in different fibers. The detection limit of the fabricated system is 5.08×103 cfu/ml for Vibrio antigen and $0.1{\mu}g/ml$, $0.05{\mu}g/ml$ in non-labeled monolayer phosphate buffered saline (NMP), non-labeled monolayer carbonate bicarbonate buffer (NMC), respectively.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.206-209
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• 2018

According to data released by the WHO(World Health Organization), the death toll from indoor air pollution in 2016 was about 3.8 million. While many people are aware of outdoor air pollution, indoor air pollution is getting less attention. Recently, however, indoor air pollution has also become a big problem, raising many issues. Frequent indoor activities such as cooking and heating produce various pollutants, which can cause children and elderly with weak immune systems to be exposed to pollutants, which can cause serious health problems such as poor lung function and respiratory diseases. Therefore, in this paper, the IoT(Internet of Things) platform that measures and monitors indoor air quality using AQ(Air Quality) sensors is developed so that data can be monitored in real time through applications and reactive web.