• Title/Summary/Keyword: 매개효과 분석

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Relationship Among Self-Determination, Entrepreneurship and Entrepreneurial Intention of University Students in Korea (예비창업자의 자기결정성이 기업가정신과 창업의지에 미치는 영향: 창업교육의 조절효과를 중심으로)

  • Kim, Sung Hwan;Bok, Kyoung Soo;Park, Jae Choon
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.10 no.5
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    • pp.15-26
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    • 2015
  • Overall, college students tend to be immersed in the relatively safe employment, such as civil servants or public sector than start one's own business due to the lack of entrepreneurial spirit which made the great success in order to take the risk and innovative challenge. In this study, relationship between pre-founder of college students' self-determination, entrepreneurship, entrepreneurial intentions are to be determined. As a result, the implications were derived in order to make pre-founder various ways of career decision beside employment. Therefore start-up training courses was verified by moderating effects. The results are first, high self-determination(Autonomy, Efficacy) of the pre-founder made positive impact on entrepreneurial intention. Second, high self-determination(Autonomy, Efficacy) of the pre-founder made positive impact on entrepreneurship. Third, entrepreneurship of the pre-founder made positive impact on entrepreneurial intention. Fourth, entrepreneurship was carried out a full mediating role in the relationship between self-determination and entrepreneurial intention. Finally, Pre-founder group who have taken entrepreneurship education have showed a positive relationship between high self-determination(Autonomy, Competence, Relationship) and entrepreneurial intention. Based on these results, especially pre-founders, in order to improve the willingness of university students entrepreneurship, highly motivated intrinsic motivation is needed to ensure the student's self-determination in University education. It looks that can be achieved through the strengthening of entrepreneurship education for autonomy, competence, efficacy improvement.

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S-allylcysteine-mediated Activation of Caspases and Inactivation of PARP to Inhibit Proliferation of HeLa (S-allylcysteine 매개 caspases의 활성화 및 PARP의 불활성화를 통한 HeLa 세포주의 증식 억제효과)

  • Kim, Hyun Hee;Kong, Il-Keun;Min, Gyesik
    • Journal of Life Science
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    • v.27 no.2
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    • pp.164-171
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    • 2017
  • Our previous study suggested that S-allylcysteine (SAC) inhibits the proliferation of the human cervical cancer cell line, HeLa, at least in part through the induction of apoptosis and cell cycle arrest. To further analyze the specific molecular mechanism(s) by which SAC mediates its antiproliferative effects, this study examined the role of SAC in regulating the protein expression of initiator caspase (caspase-9), effector caspases (caspase-3 and caspase-7), and poly-ADP-ribose polymerase (PARP) in HeLa. Western blot analysis showed that when cells were treated with 50 mM SAC for 48 hr, the expression of procaspase-3, -7, and -9 and PARP was reduced by 94%, 38%, 95%, and 64%, respectively, as compared to the untreated control. In contrast, the expression of caspase-3, -7, and -9 and cleaved-PARP was markedly increased by SAC treatment. The SAC-mediated changes in the expression of these proteins were correlated with the concomitant inhibition of cellular proliferation by SAC. The cell proliferation assay showed that HeLa treatment with more than 20 mM SAC for 6-48 hr resulted in both concentration- and time-dependent inhibition of cellular proliferation. These results indicate that the SAC-induced antiproliferative effect in HeLa may be mediated at least in part through the activation of caspase-9, followed by the activation of caspase-3 and caspase-7 as well as the inactivation of PARP, thus leading to cellular apoptosis.

Transformation of Plant Cells by Gene Transfer : Construction of a Chimeric Gene Containing Deleted Maize Alcohol Dehydrogenase Intron and ${\beta}-Glucuronidase$ Gene and Its Expression in Potato (유전자 도입에 의한 식물세포의 형질전환 : 옥수수 알코올 탈수소효소 유전자의 절단된 인트론 및 ${\beta}-Glucuronidase$ 유전자를 함유하는 키메라 유전자의 제조와 감자에서의 발현)

  • 이광웅
    • Journal of Plant Biology
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    • v.35 no.3
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    • pp.237-245
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    • 1992
  • To understand the properties of the cauliflower mosaic virus (CaMV) 35S promoter and the effect of the deleted maize alcohol dehydrogenase I-S (Adhl-S) intron 1 on the expression of the CaMV $35S{\beta}-glucuronidase$ (GUS) gene in potato (Solanum tuberosum L. cv. Superior), we constructed a chimeric gene and transferred it into potato with Agrobacterium tumefaciens mediated method. The pLS201, a gene transfer vector of 17.7 kilobase pairs, was composed of the CaMV 35S promoter, the 249 base pairs of deleted maize Adhl-S intron 1, the GUS reporter gene, and the kanamycin resistance gene as a selectable marker for transformation. The GUS activity was examined by histochemical and spectrophotometric assay in transformed potato plants. The GUS activity was found primarily around the vascular tissue cells in stem and root. In the spectorophotometric assay, the level of GUS activity of transgenic potato transformed with CaMV 35S/249 bp of intron 1 fragment-GUS (pLS201) was compared with that of potato transformed with CaMV 35S-GUS (pBI121). The quantitative spectrophotometric assay showed that the level of GUS activity in potato transformed with pLS201 was higher in leaf, stem and root by 30-, 34- and 42-fold, respectively than those in potato transformed with pBI121. This results indicate that the inclusion of the deleted maize Adhl-S intron 1 resulted in increament of the GUS gene expression in transgenic potato.potato.

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A Seismic Behavior of a 3-dimensional Irregular Setback Structure (3차원 비정형 Setback 구조물의 지진 거동)

  • 문성권
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.13 no.1
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    • pp.105-113
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    • 2000
  • Seismic behavior of 3-dimensional setback structures showing abrupt reductions of the floor size within the structure height and the effect of in-plane deformations of floor slabs on the seismic behavior of those structures are investigated. To find out general seismic behavior of 3-dimensional setback structures two parameters, level of setback(L/sub s/) and degree of setback(R/sub s/) are used. Analysis results obtained from forty eight setback structures show that a sudden change in story shear near setback level is occurred for irregular setback structures. The effect of in-plane deformation of floor slabs on the seismic behavior of setback structures is greatly influenced by the arrangement of lateral load resisting elements and it is more pronounced for frame-shear wall system showing large difference in stiffness among the lateral load resisting elements. The in-plane deformation of floor slabs results in reduced base shear, especially for FW-type structures with L/sub s/=1.0. Also, it brings about reduced story shear for the lateral load resisting element with shear wall and increase in story shear lot the lateral load resisting element without shear wall. The in-plane deformation of floor slabs at the base portion and/or tower portion due to difference in stiffness among the lateral load resisting elements brings about increment of floor displacements at all floor level.

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The Parallelization Effectiveness Analysis of K-DRUM Model (분포형 강우유출모형(K-DRUM)의 병렬화 효과 분석)

  • Chung, Sung-Young;Park, Jin-Hyeog;Hur, Young-Teck;Jung, Kwan-Sue
    • Journal of Korean Society for Geospatial Information Science
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    • v.18 no.4
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    • pp.21-30
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    • 2010
  • In this paper, the parallel distributed rainfall runoff model(K-DRUM) using MPI(Message Passing Interface) technique was developed to solve the problem of calculation time as it is one of the demerits of the distributed model for performing physical and complicated numerical calculations for large scale watersheds. The K-DRUM model which is based on GIS can simulate temporal and spatial distribution of surface flow and sub-surface flow during flood period, and input parameters of ASCII format as pre-process can be extracted using ArcView. The comparison studies were performed with various domain divisions in Namgang Dam watershed in case of typoon 'Ewiniar' at 2006. The numerical simulation using the cluster system was performed to check a parallelization effectiveness increasing the domain divisions from 1 to 25. As a result, the computer memory size reduced and the calculation time was decreased with increase of divided domains. And also, the tool was suggested in order to decreasing the discharge error on each domain connections. The result shows that the calculation and communication times in each domain have to repeats three times at each time steps in order to minimization of discharge error.

Rehabilitation assistive technology in adaptation to disabled job Effect on the use of research (장애인 직무적응에 대한 재활보조공학 이용 효과 연구)

  • Jeong, S.H.
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.7 no.1
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    • pp.59-66
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    • 2013
  • This study rehabilitation assistive technology system for people with disabilities employed by a company in the field of occupations and job satisfaction have adapted to, and for the rehabilitation assistive technology support(rehabilitation assistive technology hardware and the software) and service quality based on the quality of convenient for employees to work life by analyzing the factors that can act on adaptation employees on behavioral intentions was to determine the overall impact. Seoul, Gyeonggi, Incheon companies based in vocational education and training received in the employment of the disabled subject questionnaires were distributed, and finally 594 valid questionnaires were minor. In order to test the hypothesis SEM(structural equation model) were used, the results of this study can be summarized as follows. First, rehabilitation assistive technology hardware quality of the quality of rehabilitation assistive technology software affected. Second, rehabilitation assistive technology software quality on the quality of the service quality affected. Third, rehabilitation assistive technology hardware quality on the quality of the service quality affected. Fourth, quality of service, the quality of the adaptation of action for employees affected also. Fifth, rehabilitation assistive technology software for adaptive quality of the employees also had an impact on behavior. Sixth, rehabilitation assistive technology hardware to adapt the quality of the employees affected. And parameters (quality of service quality) influenced to as indirect effects. The results of this study support the rehabilitation assistive technology and rehabilitation assistive technology hardware and software) based, quality of service and quality of fused form acceptable to, the degree of action for employees to adapt more implications that may affect have provided.

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The Effects of the Supervisory Trust and Personal Characteristics on the Salesperson's Efforts and Performance (개인특성과 상사의 신뢰가 판매원의 노력 및 성과에 미치는 영향)

  • Kim, Yi-Tae
    • Journal of Global Scholars of Marketing Science
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    • v.11
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    • pp.21-41
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    • 2003
  • This study verified how the salesperson's efforts have an effect upon the result and the self-effect, the competitive mind, the role accuracy, supervisory trust in the important factors that effect the salesperson's efforts. After making up a questionary centering around the large distribution company and analyzing 218 copies, the result are as follows; First, salesperson's working hard has an affirmative effect upon the performance in the salesperson's efforts. Second, the self-effect, the competitive mind, supervisory trust have an affirmative effect in the working hard, and self-effect, supervisory trust have an affirmative effect in the working hard. But the role accuracy has a negative effect. Third, the self-effect, the competitive mind, the role accuracy, and the supervisory trust in the salesperson's performance are ineffectual. But, in the general effect, the self-effect has an affirmative effect and the role accuracy has a negative effect. The individuality and the supervisory trust don't have an effect upon the performance but the effect can rise through the salesperson's efforts, Therefore company should give salespersons the chance they can work hard, and would provide them the concrete aim rather than say to work hard only.

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A Study on the Tidal Energy Yield Capability according to the Yaw Angle in Jangjuk Strait (장죽수도에서의 요각변화에 따른 조류에너지 생산량에 관한 연구)

  • Tran, Bao Ngoc;Choi, Min Seon;Yang, Changjo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.7
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    • pp.982-990
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    • 2019
  • The interest of researchers and governments in exploiting tidal energy resources is increasing. Jangjuk strait is a place with high tidal energy density potential and is therefore appropriate for the constructing of a tidal turbine farm. In this study, a numerical approach is presented to evaluate the current flow and power potential in Jangjuk strait with an ADCIRC model. Then, the tidal field characteristics are utilized as input parameters for tidal resource calculation with an in-house program. The 1 MW scale tidal energy converter devices are employed and arranged in 4 layouts to investigate the annual energy yield as well as flow deficit due to the wake ef ect at the surveyed area. The best-performed array generates an annual energy yield up to 12.96 GWh/year (without considering the wake effect); this value is reduced by 0.16 GWh/year when accounting for the energy loss caused by the flow deficit. Moreover, by altering the turbine yaw angle during the flood and ebb tides, the impacts of this factor on the energy extraction are analyzed. This indicates that the turbine array attains the maximum tidal power when the turbine yaw angle is at 346° and 164° (clockwise, to the North) for the spring and neap tide in turns.

Non-point Souce Quantative Analysis Using Watershed model in Nakdong River (HSPF 모형을 이용한 낙동강의 비점오염원 정량화 기법 연구)

  • Kim, Dong-Il;Kim, Kwang-Moon;Han, Kun-Yeun;Park, Tae-Won
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.782-782
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    • 2012
  • 지금까지 우리나라에서는 도시하수, 공장폐수 등의 점오염원에 국한하여 중점적으로 수질관리를 실행하여 부분적으로 효과를 얻을 수 있었으나, 하천과 호소의 수질은 크게 향상되지 않고 있다. 이는 급속한 도시화와 산업발달로 토지개발이 가속화되고 대지, 도로, 주차장 등 불투수층 면적이 늘어남에 따라 비점오염원에 의한 하천, 호소의 수질영향도가 커지고 있기 때문이다. 인구증가로 인해 물 사용량 뿐만 아니라 이에 따라 배출되는 오염원의 종류 및 오염부하량 역시 함께 증가하고 있다. 장래의 수질관리 성공여부는 비점오염원의 효율적인 관리여부가 큰 변수로 작용할 것으로 본다. 따라서 공공수역의 수질관리를 위해서는 토지이용과 지역특성을 고려한 비점오염원 부하량의 합리적인 조사, 오염 부하량 절감을 위한 관리기술의 개발, 비점오염원 관리정책의 개발 및 수질모형을 이용한 정확한 수질예측 등이 필요하다. 따라서 본 연구에서는 공간정보를 바탕으로 한 낙동강 유역에서의 비점오염원 정량화 분석을 수행하고자 한다. 우선 대상유역으로 낙본 G유역을 선정하여 이에 대한 조사를 통해 점오염원의 실측자료를 구축하고 이를 HSPF의 입력하여 모의를 수행하여 대상유역에 대한 실측치를 이용해 모형의 보정과 검증을 수행한다. 이러한 과정을 통해 도출된 결과는 대상유역의 총 오염량을 의미한다. 따라서 위의 과정에서 도출된 매개변수를 이용하고, 점오염원을 제거한 뒤 모의를 재수행하여 나온 결과가 대상유역의 비점오염원의 양이라 판단하였다. 모의 결과 대상유역인 낙본 G유역에서 약 39% 정도의 비점오염원 비율을 보였다. 그러나 수질 및 유량 관측치를 지금까지는 국립환경과학원 낙동강물환경연구소 유량측정데이타를 사용하고 있는데 이 자료는 8일 이상 간헐적으로 측정이 수행되고 있다. 따라서 검 보정 대상이 되는 실측치의 자료의 부족과 부정확한 유역이 있음이 한계점으로 작용한다. 그러므로 추후의 신경망 모형이나 기타 실측치 보간에 있어서의 신뢰도를 높이는 기법 개발이나 측정제도의 보편적인 기술의 증대도 앞으로의 모델링에 있어서 중요할 것으로 판단된다. 또한 유역수질모형의 모델링 과정에서 좀 더 신뢰도 높은 측정자료와 그 측정자료를 활용하여 PEST 보정기법을 적용한다면 더욱 정확한 예측이 이루어질 수 있을 것이며, 본 연구에서의 평가방법을 바탕으로 유역수질모델링이 이루어진다면 보다 더 정확성 높은 비점오염원 정량화와 수질 예측이 수행될 수 있을 것이며 더 나아가 오염총량제의 수행에 효과적으로 적용될 것으로 판단된다.

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The anti-inflammatory influence of fermented soy products containing a fermented Rhus verniciflua extract on lipopolysaccharide (LPS)-treated RAW 264.7 cells (LPS 유도 RAW264.7세포에서 발효 옻 추출물을 함유한 장류의 항염증 효과)

  • Lim, Hyun Ji;Kim, Hyoun-Young;Lee, Jeong-Mi;Kim, Hyun Ju
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.642-652
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    • 2018
  • Rhus verniciflua (RV) Stokes is a herbal medicine that helps improve blood circulation by stimulating digestion, removing extravasated blood, and raising body temperature. The purpose of this research was to study the anti-inflammatory effect of fermented soy products (FSP) containing a fermented RV (FRV) extract on lipopolysaccharides (LPS)-treatedd RAW 264.7 cells. Treatment with FRV extracts (1, 10, $100{\mu}g/mL$) downregulated nitric oxide (NO) and pro-inflammatory cytokines as compared to the LPS-treated group. Besides, the RV extract treatment suppressed the expression of genes related to pro-inflammatory cytokines, matrixins, inflammation, and apoptosis, while increasing the expression of genes involved in the antioxidant system. Furthermore, RVS extract upregulated antioxidant enzymes, such as glutathione, Cu,Zn-SOD, and catalase without changes in the Nrf2-Keap1 pathway. FSP (doenjang, ganjang) containing FRV extracts (0.1, 1, or $10{\mu}g/mL$) significantly decreased the NO and IL-6 levels in an FSP after 8 weeks of fermentation, but not the expression of genes involved in the inflammation and antioxidant system. These result indicate that an FRV extract and FSPs have a potential application in inflammatory conditions.