• Proceedings of the Korean Information Science Society Conference
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• 2005.11b
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• pp.247-249
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• 2005

마이크로어레이 기술로 인해 많은 양의 유전자 발현 데이터가 생겨났다. 데이터의 양이 많아짐에 따라 이를 체계적으로 관리해야 할 필요가 생겼고 이를 위해 LIMS(Laboratory Information Management System)가 만들어졌다. LIMS는 실험 데이터를 체계적으로 관리할 뿐만 아니라 같은 실험실에 있는 연구자 간에 데이터를 공유할 수 있는 장점을 갖고 있다. 만일 여러 연구실 간에 공동연구를 한다면 데이터의 공유가 필요하게 된다. 이를 좀 더 편리하게 하기 위해 다른 연구실의 LIMS에 저장된 데이터 중에 허용된 데이터에 한해서 자신의 LIMS에서 바로 접근할 수 있도륵 하는 분산 LIMS를 소개하고자 한다.

• The Korean Journal of Applied Statistics
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• v.26 no.3
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• pp.389-399
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• 2013

This article introduces Gaussian process regression and shows its application with time-course microarray gene expression data. Gene screening for yeast cell cycle microarray expression data is accomplished with a ratio of log marginal likelihood that uses Gaussian process regression with a squared exponential covariance kernel function. Gaussian process regression fitting with each gene is done and shown with the nine top ranking genes. With the screened data the Gaussian model-based clustering is done and its silhouette values are calculated for cluster validity.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2006.11a
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• pp.83-86
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• 2006

많은 유전자 정보와 그 부산물은 많은 방법을 통해 연구되어 왔다. DNA 마이크로어레이 기술의 사용은 많은 데이터를 가져왔으며, 이렇게 얻은 데이터는 기존의 연구 방법으로는 분석하기 힘들다. 본 눈문에서는 많은 양의 데이터를 처리할 수 있게 하기 위하여 K-means 클러스터링 알고리즘을 이용한 분할 클러스터링을 제안하였다. 제안한 방법을 쌀 유전자로부터 나온 마이크로어레이 데이터에 적용함으로써 제안된 클러스터링 방법의 유용성을 검증하였으며, 기존의 K-means 클러스터링 알고리즘을 적용한 결과와 비교함으로써 제안된 알고리즘의 우수성을 확인 할 수 있었다.

• The Korean Journal of Applied Statistics
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• v.18 no.2
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• pp.355-369
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• 2005

Genome sequencing and microarray technology produce ever-increasing amounts of complex data that needs statistical analysis. Visualization is an effective analytic technique that exploits the ability of the human brain to process large amounts of data. In this study, biplot approach applied to microarray data to see the relationship between genes and samples. The supplementary data method to classify new sample to known category is suggested. The methods are validated by applying it to well known microarray data such as Golub et al.(1999), Alizadeh et al.(2000), Ross et al.(2000). The results are compared to the results of several clustering methods. Modified graph which combine partitioning method and biplot is also suggested.

• The KIPS Transactions:PartD
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• v.15D no.6
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• pp.767-776
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• 2008

Microarray technology is extensively being used in experimental molecular biology field. Microarray experiments generate quantitative expression measurements for thousands of genes simultaneously, which is useful for the phenotype classification of many diseases. One of the two major problems in microarray data classification is that the number of genes exceeds the number of tissue samples. The other problem is that current methods generate classifiers that are accurate but difficult to interpret. Our paper addresses these two problems. We performed a direct integration of individual microarrays with same biological objectives by transforming an expression value into a rank value within a sample and generated rank-comparison decision rules with variable number of genes for cancer classification. Our classifier is an ensemble method which has k top scoring decision rules. Each rule contains a number of genes, a relationship among involved genes, and a class label. Current classifiers which are also ensemble methods consist of k top scoring decision rules. However these classifiers fix the number of genes in each rule as a pair or a triple. In this paper we generalized the number of genes involved in each rule. The number of genes in each rule is in the range of 2 to N respectively. Generalizing the number of genes increases the robustness and the reliability of the classifier for the class prediction of an independent sample. Also our classifier is readily interpretable, accurate with small number of genes, and shed a possibility of the use in a clinical setting.

• Journal of the Korean Institute of Intelligent Systems
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• v.22 no.6
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• pp.786-792
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• 2012

A microarray is a collection of thousands of DNAs or RNAs arranged on a substrate, and it enables one to navigate large amounts of gene expression. However, a researcher uses his designed experimental methods to focus on particular phenotypes from the available mass of data. In this paper, we used MicroRNAs(miRNAs) and Protein-Protein Interation(PPI) databases to enhance and expand meanings in microarray data. Further, the expanded data are linked with the Online Mendelian Inheritance in Man(OMIM), and International Statistical Classification of Diseases and Related Health Problems, $10^{th}$ Revision(ICD-10), in order to extract common genetic relationships between diseases. This approach, we expect, should provide new biological views.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2005.05a
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• pp.198-201
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• 2005

DNA microarray 칩은 신약 개발, 유전적 질환 진단, Bio-molecular 상호작용 연구, 유전자의 기능연구 등 폭넓게 사용되고 있다. 이 논문은 cDNA mimcroarray 데이터를 분석하기 위한 웹형태의 시스템 개발에 대한 내용을 다룬다. 하나의 cDNA microarray에는 수 백에서 수 만개의 유전자가 심어져 있으며, 데이터를 분석할 때 대량의 데이터와 다양한 형태의 오류로 인해서 데이터간의 차이를 보정하는 분석 도구와 통계적 기법들이 사용되어야 한다. 본 논문에서는 가상 칩 뷰어를 이용하여 실제 microarray 데이터의 foreground intensity에서 백그라운드의 intensity를 제거하여 일반화된 칩 이미지를 생성한다. 이 가상 칩 뷰어는 여러 가지 필터효과와 서로 다른 두 형광의 차이를 조정하는 global normalization 기법을 사용하여 발현 유전자 분석을 시각적으로 할 수 있고, 중복된 마이크로어레이 칩 데이터를 통하여 시간이 많이 걸리는 분석전 칩의 유효성을 검토할 수 있다. 칩 데이터의 normalization을 위한 통계 방법으로 R 통계 도구와 linear 모델을 사용하여 microarray 칩의 유전자 발현 양상을 분석한다. 통계적 방법을 사용하지 않은 데이터를 추출, 이 데이터의 패턴 그래프 그리고 발현 레벨을 분류하여 마이크로어레이의 각 스팟의 유효성 검토의 정확성을 높였다. 이 시스템은 칩의 유효성 검토, 스팟의 유효성 검토, 유전자 선정에 대해 분석의 용이성과 정확성을 높일 수 있었다.

• Journal of KIISE:Computer Systems and Theory
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• v.29 no.7
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• pp.411-421
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• 2002

In this Paper we propose a new Image analysis algorithm for microarray processing and a method to locate the position of the grid cell using the topology of the grid spots. Microarray is a device which enables a parallel experiment of 10 to 100 thousands of test genes in order to measure the gene expression. Because of the huge data obtained by a experiment automated image analysis is needed. The final output of this microarray experiment is a set of 16-bit gray level image files which consist of grid-structured spots. In this paper we propose one algorithm which located the address of spots (spot indices) using graph structure from image data and a method which determines the precise location and shape of each spot by measuring the inclination of grid structure. Several experiments are given from real data sets.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.11
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• pp.2039-2044
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• 2006

Since the bio-information now even exceeds the capability of human brain, the techniques of data mining and artificial intelligent are needed to deal with the information in this field. There are many researches about using DNA microarray technique which can obtain information from thousands of genes at once, for developing new methods of analyzing and predicting of diseases. Discovering the mechanisms of unknown genes by using these new method is expecting to develop the new drugs and new curing methods. In this Paper, We tested accuracy on classification of microarray in Bayesian method to compare normalization method's Performance after dividing data in two class that is a feature abstraction method through a normalization process which reduce or remove noise generating in microarray experiment by various factors. And We represented that it improve classification performance in 95.89% after Lowess normalization.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.10
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• pp.1924-1929
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• 2007

Nowadays, a lot of related data obtained from these research could be given a new present meaning to accomplish the original purpose of the whole research as a human project. The method of tumor classification based on microarray could contribute to being accurate tumor classification by finding differently expressing gene pattern statistically according to a tumor type. Therefore, the process to select a closely related informative gene with a particular tumor classification to classify tumor using present microarray technology with effect is essential. In this thesis, we used cDNA microarrays of 3840 genes obtained from neuronal differentiation experiment of cortical stem cells on white mouse with cancer, constructed accurate tumor classification model by extracting informative gene list through normalization separately and then did performance estimation by analyzing and comparing each of the experiment results. Result classifying Multi-Perceptron classifier for selected genes using Pearson correlation coefficient represented the accuracy of 95.6%.