• Korean Journal of Soil Science and Fertilizer
• /
• v.37 no.4
• /
• pp.266-287
• /
• 2004

The non infecting, plant associated bacteria have attracted increased attention for stimulating plant growth and as environmental friendly plant protecting agents. Pink-pigmented facultatively methylotrophic bacteria (PPFMs), classified as Methylobacterium spp., are persistent colonizers of plant leaf surfaces. As the leaves of most or all plants harbor PPFMs that utilize leaf methanol as their sole source of carbon and energy, which is a specific attribute of the genus Methylobacterium. Although they are not well known, these bacteria are co-evolved, interacting partners in plant metabolism. This claim is supported, for example, by the following observations: (1) PPFMs are seed-transmitted, (2) PPFMs are frequently found in putatively axenic cell cultures, (3) Low numbers of seed-borne PPFMs correlate with low germinability, (4) Plants with reduced numbers of PPFM show elevated shoot/root ratios, (5) Foliar application of PPFMs to soybean during pod fill enhances seed set and yield, (6) Liverwort tissue in culture requires PPFM-produced vitamin B12 for growth, (7) treated plants to suppress or decrease disease incidence of sheath blight caused by Rhizoctonia solani in rice, and (8) the PPFM inoculation induced number of stomata, chlorophyll concentration and malic acid content, they led to increased photosynthetic activity. Methylobacterium spp. are bacterial symbionts of plants, shown previously to participate in plant metabolism by consuming plant waste products and producing metabolites useful to the plant. There are reports that inform about the beneficial interactions between this group of bacteria and plants. Screening of such kind of bacteria having immense plant growth promoting activities like nitrogen fixation, phytohormone production, alleviating water stress to the plants can be successfully isolated and characterized and integration of such kind of organism in crop production will lead to increased productivity.

• Journal of the Korean GEO-environmental Society
• /
• v.10 no.5
• /
• pp.19-26
• /
• 2009

Rammed Aggregate Pier (RAP) method is intermediate foundation between deep and shallow foundation, and it has been built in world wide. RAP represents a relatively new method that has grown steadily over 19 years since Geopier of USA developed this revolutionary method in 1989. The investigation and research in domestic is not accomplished. In this paper, the examined details of different spacing of piles, bearing capacities, respectively, conclude with recommendations on how RAP can be used in future needs. This documentation further provides comparisons of the laboratory test results which were obtained from changing the spacing of piles, namely installed rammed aggregate pier. Laboratory model test was administered in a sand box. Strain control test was conducted to determine the bearing capacities of the piers; 20 mm, 30 mm and 40 mm RAP in diameter using drilling equipment to make holes were installed in sand at initial relative densities of 40%. By comparing different spacing of piles, in this experiment, piles are spaced structually span, form a ring shape, narrowing the distance of each other, to the center. the result shows that as diameter of pier is bigger in diameter, bearing capacity also dramatically increased due to raised stiffness. Also, as the space between each piers was closed, the settlement rate of soil was decreased significantly. From the test results, as the space between each piles were getting closer, it allows greater chances to have more resistance to deformation, and shows more improved stability of structures. After from the verification work which is continuous leads the accumulation of the site measuring data which is various, and bearing capacity and the settlement is a plan where the research will be advanced for optimum installed RAP.

• Journal of the Korean Society of Costume
• /
• v.52 no.1
• /
• pp.53-67
• /
• 2002

자연을 분석함으로써, 얻어진 기하학적 패턴은 이미 자연의 질서를 포함하고 있는 논리적이고 합리적인 기본형이기 때문에 간결하며 시각적으로 명쾌감을 준다. 이러한 기하학적 패턴은 복식 디자인에 있어서 20 세기 이후 여러 디자이너의 작품을 통해 재구성되어 현대적 이미지를 나타내는 중요한 모티브가 되고 있으며, 다양한 기법과 재료로 형성화하여 도입되고 있다. 이에 본 연구는 복식의 문양, 실루엣, 디테일에 사용되고 있는 기하학적 패턴을 연구함으로써 기하학적 패턴의 새로운 조형가치를 고찰하였다. 먼저 기하학의 용어 정의를 하였고 기하학적 패턴의 유형과 표현 기법을 분석하고 정리하여 현대 패션에 나타난 기하학적 패턴의 조형미와 그것을 바탕으로 패션 이미지를 추론해 보았다. 현대 패션에 나타난 기하학 패턴을 분석해 보면 유형으로는 첫째, 기하하적 문양으로 복식디자인에 있어서 주로 평면적인 형태로 많이 나타나지만, 크기가 다르고 동일한 기하학적 패턴을 조합시킴으로서 평면적인 형태에 공간감을 부여하기도 하며, 같은 기하학적 패턴의 표면이라도 배치구조에 의해 직선 혹은 사선으로 지각되므로 전혀 다른 이미지를 주었다. 또한 현대 패션에 나타난 기하학적 패턴이 종류는 세로 스트라이프, 가로 스트라이프, 격자 문양, 원, 사선 스트라이프, 마름모, 사각형, 삼각형 등의 순서로 많이 나타났다. 둘째, 색채는 단색의 복식에 강한 대비가 이루어지는 색상으로 표현되어 역동감과 유연한 운동감을 나타났다. 셋째, 기하학적 실루엣으로 단순한 라인의 형태를 나타내거나 입체적이고 부조적인 형태로 구성되어 전체적인 실루엣으로 사용되어 강한 조형감각을 보여주는데 원형을 이용한 실루엣이 가장 많았으며 사각형을 이용한 실루엣, 삼각형을 이용한 실루엣 순서로 나타났다. 넷째, 기하학적인 디테일로 복식의 어느 한 부분에 장식적으로 사용되거나 입체적 형태로 부출 되어 부조적인 느낌을 주는데 소매에 가장 많이 나타났으며 앞여밈, 칼라, 밑단, 주머니 순서로 장식되었다. 다섯째, 현대 패션에 표현된 기하학적 패턴의 표현기법으로는 프린팅, 퀼팅, piece기법, 패치워크, 엮기, 꼴라쥬, 아플리케 순서로 많이 나타났다. 위의 분석을 토대로 기하학 패턴을 활용한 디자인에 내재된 조형의지는 다음과 같이 정리되었다. 첫째, 기하학적 패턴이 지닌 단순성과 경직성을 완화하기 위하여 여러 가지 패브릭을 조합시켜 입체적인 표면효과로 시각적인 착시효과를 극대화하였다. 둘째, 표현기법은 입체파적 표현주의의 특성의 하나로 복시에 사용되는 소재의 왜곡으로 설명할 수 있으며, 새롭고 실험적인 소재의 도입으로 인해 의외성과 부조화를 유발시키는 통시에 유희직인 일면도 지니는 일종의 그로테스크를 나타냈다. 이상에서 정립된 조형의지를 바탕으로 현대 패션에 나타란 기하학 패턴은 절제된 단순함과 명확성으로 단순미가 유추되었고 강한 색상대비로 인한 시각적 집중효과로 주목성을 가지며 재현이 가능하므로 반복성이 유추되었다. 그리고 표준영역이 없는 창의적 표현으로 풍부한 독창성을 보여주고 있다. 또한 내재된 패션 이미지를 분석해 보면 정확함과 차가움의 의미를 지닌 이지적 이미지와 우주의 질서를 반영하는 상징적 이미지, 복잡한 자연으로부터 간결한 형태로의 경향성이 이루어낸 인공적 이미지를 느낄 수 있었으며, 미래적 이미지와 전통적 이미지의 상반된 개념의 이미지를 같이 내포하고 있음을 추론할 수 있었다. 이와 같이 현대 패션에 표현된 기하학적 패턴은 복식을 조형예술 분야로 확실히 인식시키고 발전시키는 데 중요한 촉매제 역할을 담당하고 있으며 또한 많은 디자이너들에게 창조적 욕구를 불러일으키고 영감을 주는데 중요한 모티브를 제공하고 있다.

• Journal of Wetlands Research
• /
• v.19 no.2
• /
• pp.193-201
• /
• 2017

This study was carried out to obtain basic ecological information required for wetland restoration in Korea where wetland is very deficient. To arrive at the objective, we collected the basic ecological information for wetland restoration in four Doombeongs located on Goesan of Chungcheongbuk-do (province), central Korea where maintains relatively integrate feature of Doombeong. Synthesized horizontal distribution of vegetation based on vegetation established naturally except that established by artificial interference, Potamogeton distinctus community, Spirodela polyrhiza community, Sagittaria aginashi community, Trapa japonica community, Scirpus triangulatus community, and Sparganium japonicum community, Persicaria thunbergii community, Juncus effusus var. decipiens community, Ludwigia prostrata community, Humulus japonicus community, Persicaria nodosa community, Miscanthus sacchariflorus community, Phragmites communis - P. japonica community, and Scirpus radicans community, S. gracilistyla community, Spiraea prunifolia for. simpliciflora community, and Rosa multiflora community, and Salix koreensis community and Acer tataricum subsp. ginnala community tended to be established in aquatic zone, herbaceous plant dominated vegetation zone, shrub dominated vegetation zone, and tree and sub-tree dominated vegetation zone, respectively. As the result of DCA ordination based on vegetation data collected from several Doombeongs and their surrounding areas, plant communities tended to be classified into aquatic, wetland, and riparian plant dominated stands. Spatial niche and species composition of major plant communities composing those stands were suggested as the reference information for creating ecological pond as a type of wetland. Further, the importance of wetland and the necessity of wetland restoration was discussed based on functions that the wetland displays.

• The Korean Journal of Pesticide Science
• /
• v.11 no.1
• /
• pp.32-37
• /
• 2007

The study was carried out to establish buffer zone for the protection of the non-target organisms by aerial pesticide application. The two pesticide combination of 3-way tank-mixing of three pesticides for aerial application, ferimzone tricyclazole SC + BPMC EC + validamycin-A SL and hexaconzole EC + isoprothiolane EC+phenthoate EC were selected for the simultaneous control of key pests on paddy rice as blast, sheath blight, brown planthopper and moth. Aquatic organisms including killifish and loach in the paddy field and nearby water reservoirs were not affected by aerial application of the pesticides. However, all the water flea were killed, when they were exposed 10 m from the aerially sprayed site, while the water flea exposed in 30 m away from the site were not affected. Honeybees within 50 m in the wind direction and 20 m in the opposite wind direction showed a mortality of $7{\sim}100%$. Residues concentration of the pesticides in paddy water were not detectable level after six days from aerial application. Drifting distance of aerially sprayed droplet from the target area was within 30 m in the wind direction and 20 m in the opposite direction. Consequently, it was the buffer zones in the aerial pesticides application for the protection of the non-target organisms should be at least 50 m for aquatic organisms and 100 m for honeybees.

• The Korean Journal of Pesticide Science
• /
• v.16 no.3
• /
• pp.209-216
• /
• 2012

In the continued research on natural fungicides for control of plant diseases by using plant-derived products, we found that Spatholobus suberectus Dunn had a strong fungicidal activity against several plant pathogens. S. suberectus (1 kg) was extracted with 80% aqueous MeOH and then the concentrated extract was partitioned with n-hexane, EtOAc, n-BuOH and $H_2O$ successively. The four layers were tested their disease contron efficacies against 6 plant diseases such as rice blast (RCB), rice sheath blight (RSB), tomato grey mold (TGM), tomato late blight (TLB), wheat leaf rust (WLR), and barley powdery mildew (BPM). The EtOAc fraction was highly active showing over 80% control against RCB, TGM, TLB, and BPM. By using silica gel chromatography, preparative TLC and HPLC, six compounds that were expected to have antifungal activity were separated. Their chemical structures were identified as ethanone, hydroxytyrosol, epicatechin, procyanidin B2, dimethoxy daizein and formononetin by ESI-MS, $^1H$-NMR, $^{13}C$-NMR, and 2D-NMR spectroscopic analyses. The chemicals except epicatechin were first reported in S. suberectus. Study on in vitro and in vivo antifungal activities of the isolated compounds is in progress.

• Research in Plant Disease
• /
• v.12 no.1
• /
• pp.5-9
• /
• 2006

Grain discoloration, caused by several microorganisms, is a minor disease, but it is gaining importance in almost all rice growing areas of the world in recent years. Such grains are of low market value and consumption appeal in addition to their abnormal performance as seed of source of nutrient value. When the casual agents were inoculated on rice plant,'Ilmi-byeo'(at right before heading time and on heading time), the incidence of rice ear blight caused by Alternaria alternate and Nigrospora oryzae was higher in a plot inoculated at right before heading time than (in n plot inoculated) on heading time. Meanwhile, the incidence of rice ear blight caused by Bipolaris oryzae and Curvularia lunate was higher in a plot inoculated on heading time than in a plot inoculated at right before heading time. In rice quality, the ratio of imperfect grains was increased at right before heading time or on heading time by Nigrospora oryzae or Alternaria alternata, respectively. As discoloration rate of rice grains increased on 'Ilmi-byeo', the percentage of perfect grains decreased proportionally and the percentage of damaged rice grains increased remarkably.

• Applied Biological Chemistry
• /
• v.49 no.4
• /
• pp.270-275
• /
• 2006

[ $2{\beta},\;3{\alpha}$ ], 23-trihydroxyrus-12-ene-28-oic acid was isolated from Trapa pseudoincisa S. et Z. It has a common structure of pentacyclic triterpenes and belongs to the amyrin ursolic acid group. The cytotoxic effect of this compound was investigated in human hepatoma cell line HepG2. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid showed dose-dependent cytotoxicity in HepG2 cells. Confocal microscopy data showed that green fluorescence was increased in $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid treated-HepG2 cells in a time-dependent manner. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid also increased the sub-G1 cell population of HepG2 cells as well as ladder-like DNA fragmentation. Taken together, our results indicate that $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid induced apoptosis in HepG2 cells.

• Research in Plant Disease
• /
• v.15 no.2
• /
• pp.57-62
• /
• 2009

Genetic diagnosis method of Virion Captured (VC)/RT-PCR for Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV), Korean major rice viruses transmitted by small brown plant hopper, Laodelphax striatellus, was developed. Virion extraction buffer for rice plant was 0.01M potassium phosphate buffer, pH 7.0, containing 0.5% sodium sulfite. However, the extraction buffer for L. striatellus was 0.01M potassium phosphate buffer, pH 7.0, containing 0.5% sodium sulfite and 2% polyvinylpyrrolidone wt 40,000 (PVP-40). Specific primers for detection of RSV and RBSDV were selected for VC/RT-PCR method. The specific primers were used as a duplex primer to detect viruliferous small brown plant hopper collected from Gimpo, Pyeongtaek and Siheung areas in Gyeonggi province. The genetic diagnosis methods of single and duplex VC/RT-PCR for RSV and RBSDV could be used easily and economically, especially on the diagnosis of L. striatellus. The rate of viruliferous insect (RVI) for RSV was compared with ELISA and VC/RT-PCR for L. striatellus collected from fields. RVI by ELISA was same as 9.2% with RVI by VC/RT-PCR. However, there were some different detection results between the methods. It could be suggested that there is a possibility of serological and/or genomic differences among RSV isolates. The portion of RVI detected simultaneously by ELISA and VC/RT-PCR was 71.0%, and the detection rate from VC/RT-PCR was higher as 3.2% than that from ELISA, which had a reason of simultaneous detection ability both RSV and RBSDV of VC/RT-PCR.

• Research in Plant Disease
• /
• v.16 no.2
• /
• pp.129-135
• /
• 2010

PCR primers were developed to detect Pseudomonas savastanoi pv. phaseolicola, a causal agent of halo blight that occurs in all species of common bean (Phaseolus vulgaris L.), from the bean seeds. A primer set, Psp-JHF and Psp-JH-R, specifically amplified 513 bp fragment from Pseudomonas savastanoi pv. phaseolicola only. A nested primer set, psp-JH-F-ne and psp-JH-R-ne, designed from the $1^{st}$ PCR amplicon, amplified 169 bp fragment. The primer sets did not amplify any non-specific DNA from the seed extracts of Fabaceae including 4 beans, 2 soybeans, and 2 peas. The detection sensitivity of the nested PCR method developed in this study was much higher than that of ELISA and selective medium. PCR assays developed in this study should be useful to detect Pseudomonas savastanoi pv. phasolicola from the bean seeds.