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Monocentric Derivative Y Chromosome with Duplication of the SRY Gene in an Azoospermic Male (무정자증 불임남성에서 관찰된 SRY 유전자의 중복을 동반한 일동원체성 derivative Y 염색체)

  • Choi, Eun-Young;Lee, Bom-Yi;Park, Ju-Yeon;Lee, Yeon-Woo;Oh, Ah-Rum;Lee, Shin-Young;Kim, Shin-Young;Han, You-Jung;Lee, Mee-Bum;Ryu, Hyun-Mee;Seo, Ju-Tae;Park, So-Yeon
    • Journal of Genetic Medicine
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    • v.7 no.2
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    • pp.160-164
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    • 2010
  • Structural abnormalities of the Y chromosome affect normal testicular differentiation and spermatogenesis. The present case showed a rare monocentric derivative Y chromosome with partial duplication of Yp including the SRY gene and deletion of Yq12 heterochromatin. The karyotype was 46,X,der(Y)(pter${\rightarrow}$q11.23::p11.2${\rightarrow}$pter).ish der(Y)(DYZ3+,DYZ1-,SRY++), confirmed through a FISH study. Even though the patient possessed an abnormal Y chromosome, testicular biopsy showed normal testicular volumes in the proband, with gonadal hormonal levels in the normal range but bilateral varicocele and hypospermatogenesis. We speculate that the abnormal Y chromosome arose from sister chromatids during Y chromosome recombination or intra chromosomal NAHR (non-allelic homologous recombination) during meiosis in the patient's father or in the very early stages of embryogenesis. The derivative Y chromosome might interfere in the meiotic stage of spermatogenesis, leading to the developmental arrest of germ cells. The present case illustrates that the infertility phenotype can have various causes. Also, it emphasizes the importance of accurate and various genetic analyses and could aid in male infertility treatment.

$^{99m}Tc$-HMPAO-labelled Leucocyte Scintigraphy in the Diagnosis of Infection after Total Knee Replacement Arthroplasty (인공슬관절 전치환술 환자에서 $^{99m}Tc$-HMPAO-백혈구 스캔을 이용한 인공관절 감염의 진단)

  • Park, Dong-Rib;Kim, Jae-Seung;Ryu, Jin-Sook;Moon, Dae-Hyuk;Bin, Seong-Il;Cho, Woo-Shin;Lee, Hee-Kyung
    • The Korean Journal of Nuclear Medicine
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    • v.33 no.4
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    • pp.413-421
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    • 1999
  • Purpose: This study was performed to evaluate the usefulness of $^{99m}Tc$-HMPAO-labelled leucocyte scintigraphy for diagnosing prosthetic infection after total knee replacement arthroplasty without the aid of following bone marrow scintigraphy Materials and Methods: The study subjects were 25 prostheses of 17 patients (one man and 16 women, mean age. 65 years) who had total knee replacement arthroplasty. After injection of $^{99m}Tc$-HMPAO-labelled leucocyte, the whole body planar and knee SPECT images were obtained in all patients. The subjects were classified into three groups according to clinical suspicion of prosthetic infection. Group A (n=11) with high suspicion of infection; Group B (n=6) with equivocal suspicion of infection, and Group C (n=8) with asymptomatic contralateral prostheses. Final diagnosis of infection was based on surgical, histological and bacteriological data and clinical follow-up. Results: Infection was confirmed in 13 prostheses (11 in Group A and 2 in Group B). All prostheses in Group A were true positive. There were two true positives, one false positive and three true negatives in Group B, and six true negatives and two false positives in Group C. Overall sensitivity, specificity, and accuracy for diagnosis of the infected knee prosthesis were 100%, 75% and 88%, respectively Conclusion: $^{99m}Tc$-HMPAO-labelled leucocyte scintigraphy is a sensitive method for the diagnosis of infected knee prosthesis. However, false positive uptakes even in asymptomatic prosthesis suggest that bone marrow scintigraphy may be needed to achieve improved specificity.

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A Report on the Shigella Cultures Isolated in Korea (1972) (1972년(年) 한국(韓國)에서 분리(分離)된 이질균(痢疾菌)에 관(關)한 보고(報告))

  • Ryu, Young-Hat;Kim, Soon-Hee
    • The Journal of the Korean Society for Microbiology
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    • v.8 no.1
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    • pp.7-11
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    • 1973
  • The authors identified eighty-eight Shigella cultures among about four thousands specimens collected from all over the country in 1972. Of eighty-eight cultures, seventy-seven cultures belonged to Shigella flexneri and eleven cultures to Shigella sonnei. None of cultures belonging to either subgroup A or C was detected in 1972. Of seventy-seven cultures of Shigella flexneri one was $B_{1b}$, fifty-six were $B_{2a}$, nine were $B_{3a}$, six were $B_{4a}$, three were By and one was each of $B_{3b}$ and $B_{3c}$. Of eleven cultures of Shigella sonnei seven cultures appeared to be phase I and the others phase II. Although there was quite a difference found in the incidence of isolating Shigella organisms between different areas as shown in Table 1, it would not be possible to understand that there might not have been the cases or carriers of Shigella in the areas where the organisms were not isolated in 1972. Concerning the biochemical properties it was not possible to compare the results obtained from the decarboxylase and dihydrolase tests with them obtained in previous years except that of lysine decarboxylase tests since they were not reported individually by the different serotypes in the previous reports. These results obtained in 1972 would be the data for the future comparison. In regards to the antibiotics-sensitivity of Shigella cultures the most of them showed sensitive results to nitrofurantoin, ampicillin, cephalosporin, gentamycin and geopen, and the majority of them appeared to be resistant to cloxacillin, tetracycline and streptomycin by means of the In Vitro tests.

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A New Soybean Cultivar, "Galchae" for Sprout with Brown Seed Coat, Small Seed Size and High Sprout Yielding (갈색종피.소립 고수율 나물용 콩 신품종 "갈채")

  • Oh, Young-Jin;Cho, Sang-Kyun;Kim, Kyong-Ho;Kim, Young-Jin;Kim, Tae-Soo;Kim, Jung-Gon;Park, Ki-Hun;Baek, In-Youl;Han, Won-Young;Kim, Hyun-Tae;Yun, Hong-Tae;Ko, Jong-Min;Kim, Dong-Kwan;Kim, Yong-Duk;Hwang, Hung-Goo
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.324-327
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    • 2009
  • A new sprout-soybean cultivar, "Galchae" was developed at the National Institute of Crop Science (NICS) in 2008. Galchae was selected from a cross between YS1287 and Jinju#1. The preliminary, advanced, and regional yield trials to evaluate the performance of Iksan 64 were carried out from 2005 to 2008. This cultivar has a determinate growth habit with purple flower, brown pubescence, brown seed coat, brown hilum, rhomboid leaflet shape and small seed size (8.4 grams per 100 seeds). The maturity date of "Galchae" is 6 days later than the check variety, "Dawon". It has good seed quality for soybean-sprout and resistance to lodging. This cultivar has resistance to soybean mosaic virus (SMV) and necrotic symptom (SMV-N). The average yield of "Galchae" was 2.51 ton per hectare in the regional yield trials (RYT) for double cropping carried out for two years from 2007 to 2008.

Single Crossing Condition of Miscanthus sacchariflorus and Miscanthus sinensis to Breed Miscanthus x giganteus Cultivar (이질3배체 억새(Miscanthus x giganteus) 품종육성을 위한 물억새(M. sacchariflorus)와 참억새(M. sinensis) 단교배 조건구명)

  • Moon, Youn-Ho;Kim, Kwang-Soo;Lee, Ji-Eun;Kwon, Da-Eun;Kang, Yong-Ku;Cha, Young-Lok
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.509-518
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    • 2019
  • This study was conducted to investigate single crossing condition of M. sacchariflrous and M. sinensis for breeding of M. ${\times}$ giganteus cultivar. Compared with natural day length condition, cultivation in short day length condition shorten days to heading to 18~27 days in both species. Pollen germination ratio of were 75.8% at 6 o'clock in M. sacchariflorus and 51.9% at 7 o'clock in M. sinensis but decreased to below 10% at 8 o'clock in both species. When cut ears immerged in 150 mL of cut-flowers conservation solution and isolated with covering of white non-woven fabric, flowering and pollen dispersal were persisted for 7 days, and the ratio of pollen germination were above 40% for 4 days. The ratio of self-fertilization of both species were below 2.5%, but open pollenation ratio were above 50%. We obtained 437 seeds with experimental single cross of 14 combinations between tetraploid M. sacchariflorus and diploid M. siensis by application of developed single crossing methods. In the single cross, numbers of seed set were different by mother plants. Thus, the newly investigated single crossing condition will be used to breed M. ${\times}$ giganteous cultivar which is sterile and has superior characteristics of biomass yield.

$M\"{o}ssbsuer$ Effect Study of Nanocrystalline $Fe_{73.5}Cu_{1}Nb_{3}Si_{16.5}B_{6}$ Alloy (초미세결정립 $Fe_{73.5}Cu_{1}Nb_{3}Si_{16.5}B_{6}$ 합금의 뫼스바우어 효과 연구)

  • 김재경;신영남;양재석
    • Journal of the Korean Magnetics Society
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    • v.5 no.5
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    • pp.864-873
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    • 1995
  • Amorphous $Fe_{73.5}Cu_{1}Nb_{3}Si_{16.5}B_{6}$ ribbons were annealed for different time at $500^{\circ}C$ and $552^{\circ}C$, just before and after the exothermic reaction in DSC curve. The development of nanocrystalline phase was investigated by means of $M\"{o}ssbsuer$ spectroscopy. The crystalline phase consists mainly of $DO_{3}Fe-Si$. Though slight in amount (5%), another ferromagnetic phase which could be presumed $t-Fe_{3}B$ was detected Si content of $DO_{3}Fe-Si$, Si/(Fe+Si), was 0.218 under the heat treatment at $500^{\circ}C$ for 60 min and 0.222 at $552^{\circ}C$ for 10 min. Since then both of those values decreased with time until 120 min and finally these two values remained constant at 0.210. The variation in Si content with annealing time results in the variation in the hyperfine field and the isomer shift. The increase in the mean hyperfine fields and the decrease in the mean isomer shifts of Fe-Si are caused by the increase in Si content. The volume fractions of residual amorphous phase rapidly decrease during the early stage of annealing and come nearer to saturation after 120 min both at $500^{\circ}C$ and $552^{\circ}C$. The decrease in the mean hyperfine field of residual amorphous. in spite of slight changes in the volume fractions of Fe-Si and of residual amorphous after 120 min. is caused by the increase in the content of Nb and B in residual amorphous phase. The saturated volume fraction of the crystalline phase was 81% for $500^{\circ}C$ (180 min) and 77% for $552^{\circ}C$ (960 min), different from expectation.

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The Effect of Chemical Composition and Sintering Temperature on the Experiment of Physical Properties of Ni-Zn Ferrite (Ni-Zn Ferrite의 조성성분 및 소결온도에 따른 물리적 특성의 실험적 연구)

  • Koh, Jae-Gui
    • Journal of the Korean Magnetics Society
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    • v.16 no.5
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    • pp.255-260
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    • 2006
  • The basic composition of Ni-Zn ferrite was $(Ni_{0.35}Cu_{0.2}Zn_{0.45})_{1.02}(Fe_2O_3)_{0.98}$ (group A) and $(Ni_{0.4}Cu_{0.2}Zn_{0.4})_{1.02}(Fe_2O_3)_{0.98}$(group B) with additional 0.1 mol% $CaCO_3$ and 0.03 mol% $V_2O_5$. For high permeability and acceleration of grain growth, $CaCO_3$ and $V_2O_5$ was added. The mixture of the law materials was calcinated at $600^{\circ}C$ for 2 hours and then milled. The compacts of toroidal type were sintered at different temperature ($1,050^{\circ}C,\;1,070^{\circ}C,\;1,100^{\circ}C$) for 2 hours in air followed by an air cooling. Then, effects of various composition and sintering temperatures on the microstructure and physical properties such as density, resistivity, magnetic induction, coercive force, initial permeability, quality factor, and curie temperature of the Ni-Zn ferrite were investigated. The density of the Ni-Zn ferrite was $4.90{\sim}5.10g/cm^3$, resistivity revealed $10^8{\sim}10^{12}{\Omega}-cm$. The average grain size increased with the increase of sintering temperatures. The magnetic properties obtained from the aforementioned Ni-Zn ferrite specimens were 4,000 gauss for the maximum induction, 0.25 oersted for the coercive force, 2,997 for the initial permeability, 208 for the quality factor, and $202^{\circ}C$ for the curie temperature. The physical properties indicated that the specimens could be utilized as the core of microwave communication and high permeability deflection yoke of high permeability.

Effect of Green Manure Crops Incorporation with Rice Cultivation on Soil Fertility Improvement in Paddy Field (벼 재배시 녹비작물 혼입에 따른 지력개선 효과)

  • Yang, Chang-Hyu;Ryu, Jin-Hee;Kim, Taek-Kyum;Lee, Sang-Bog;Kim, Jae-Duk;Baek, Nam-Hyun;Kim, Sun;Choi, Weon-Young;Kim, Si-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.371-378
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    • 2009
  • This study was carried out to investigate the improvement effect of soil fertility by incorporation of GMC(green manure crops) at rice cropping after cultivation GMC such as the barley for alternative rye in paddy field over the past two years(2006~2007). Plots, which consisted of incorporation time of GMC as rye; heading stage, barley; heading stage, heading stage of rye and 10days after heading stage were divided by amount of applied rates; standard fertilizer fertilization, diagnosis fertilization and non-fertilization. we investigated change of soil physico-chemical properties, degree of decomposition on GMC in soil, growth and yield potential. The obtained results were summarized as follows. The fresh weight of GMC at incorporation time on heading stage of rye, heading stage and 10days after heading stage of barley were $2,715,\;2,352,\;2,867kg\;10a^{-1}$ respectively. Content of total nitrogen at three incorporation times was 1.31, 1.46, 1.38% and the C/N ratio were 33.4, 28.7, and 34.6, respectively. Some soil physical properties, such as soil hardness and bulk density tended to decrease with incorporation of GMC, while surface soil depth and porosity were increased. Some soil chemical properties, such as content of exchangeable cations and cation exchangeable capasity(CEC) were increased with incorporation of GMC compared with before experiment. Rice yields was increased 3~9% in diagnosis application plots on application of barley compared with control($559kg\;10a^{-1}$) and incorporation of barley caused to improve perfect kernel ratio 73.6~78.7% in appearance characteristics of brown rice compared with cotrol(73.0%). It was found that incorporation with 10days after heading stage of barley was more effective to reduce chemical nitrogen fertilizer and to improve soil fertility.

Features of the Military Uniforms of the Low-Ranking Soldier Belonging to Jangyongyoung in the King Jeongjo Period Seojangdaeyajodo (정조대 <서장대야조도(西將臺夜操圖)> 장용영(壯勇營) 하급 군사(軍士)의 군복(軍服) 고증)

  • LEE, Kyunghee;KIM, Youngsun;LEE, Eunjoo
    • Korean Journal of Heritage: History & Science
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    • v.54 no.4
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    • pp.90-111
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    • 2021
  • Seojangdaeyajodo is a drawing of Jangyongyoung's military night training on February 12 (lunar leap month), 1795. Focusing on the Seojangdaeyajodo, the positions and roles of the low-ranking soldier belonging to Jangyongyoung, and the composition and characteristics of military uniforms for each role were examined. The results ascertained by the historical research on the military uniforms are as follows. Deungronggun, noeja, sunryeongsu and daegisu who were placed in front of the king's Seojangdae were the low-ranking soldiers belonging to Jangyongyoung. The soldiers who escorted the king around Seojangdae were lowranking soldiers belonging to Jangyongyoung. The military uniform of the deungronggun was consisted of a jeolrip, a black heopsu, red gweja, indigo jeondae, white haengjeon and black shoes. The low-ranking soldier's heopsu suggested that it could also be a sochangui. He carried a sword and a red lantern. Noeja were divided into a sinjeonsu and a jujangsu. The military uniform of the noeja was consisted of a Jujeolrip, a black heopsu, red gweja, indigo jeondae, white haengjeon, and black shoes. Sunryeongsu were divided into a sinsigisu and a younggisu. The military uniform of the sunryeongsu was consisted of a jeongeon, a black heopsu, red gweja, indigo jeondae white haengjeon and black shoes. He carried a sword and a red lantern. The military uniform of the daegisu was consisted of a jeongeon, a black heopsu, blue gweja, indigo jeondae, white haengjeon and black shoes. He carried a sword and a flag. The soldiers surrounding Seojangdae and the seongjeonggun defending the fortress were the Chogun. The military uniform of the chogun was consisted of a jeolrip, a black heopsu, houi, indigo jeondae, white haengjeon and straw shoes. Houi was applying the five directional colors: the east is blue, the west is white, the south is red, and the north is black. He carried a sword and a gun. It was presented as an illustration of costumes that could produce contents by reflecting on these historical results. The basic principle of the illustration was to present the standards for 3D content production or actual production. Samples of form, color, and material according to the times and status were presented. The front, the side, and the back of each costume and the feature were presented, and the colors were presented in RGB and CMYK.

A Very Early-Maturing, Cold Tolerant and High Quality japonica Rice Variety 'Hanseol' (극조숙 고품질 내냉성 벼 신품종 '한설')

  • Lee, Jeong-Heui;Shin, Young-Seop;Jeong, O-Young;Kim, Myeong-Ki;Kim, Yeon-Gyu;Kim, Hong-Yeol;Lee, Jeom-Ho;Lee, Jeong-Il;Cho, Young-Chan;Jeon, Yong-Hee;Choi, Yong-Hwan;Yang, Chang-Ihn;Hong, Ha-Cheol;Won, Yong-Jae;Shin, Jin-Chul;Kim, Hyung-Yoon;Seo, Dae-Ha;Hwang, Hung-Goo;Yea, Jong-Doo
    • Korean Journal of Breeding Science
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    • v.42 no.6
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    • pp.632-637
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    • 2010
  • 'Hanseol' is a new very early-maturing, cold tolerant and high quality japonica rice variety developed from a cross of 'Jinbu24' and 'Jinbu25' by the rice breeding team of National Institute of Crop Science (NICS), Rural Development Administration (RDA) in 2009. The heading date of this variety is July 25, which is four days earlier than check variety, 'Jinbubyeo'. 'Hanseol' has 65 cm of culm length, 99 spikelets per panicle, 82.9% of ripened grain rate, and 21.5 g of 1,000 grain-weight of brown rice. This variety shows susceptibility to bacterial leaf blight and virus diseases, and insect pests. It is tolerant to cold stress in terms of less heading delay and high fertility in cold water irrigated cultivation. This variety shows delayed leaf senescence and considerable tolerance to viviparous germination at ripening stage. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and medium-short grain. 'Hanseol' showed low gelatinization temperature and 6.1% protein content, 19.1% amylose content and good palatability of cooked rice. The milled rice yield of this variety is about 5.43 MT/ha at ordinary culture in local adaptability test for three years. 'Hanseol' would be highly adaptable to mid-north and mid-mountainous areas, and mid-northern alpine area in Korea.