• Microbiology and Biotechnology Letters
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• v.16 no.6
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• pp.457-462
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• 1988

A potent actinomycetes strain was selected to digest raw starch, which was classified as a strain of Streptomyces sp.. Its amylase production was maximized when it was grown on wheat bran extract media added 4% of cooked corn starch and 0.16% of potassium nitrate for 6 days at 3$0^{\circ}C$ and initial pH 6.2.

• Food Science and Preservation
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• v.23 no.3
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• pp.413-421
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• 2016

The aim of this study was to investigate physicochemical properties of porcine blood hydrolyzed by proteases under various conditions for utilization as a food source. Five kinds of proteases (Alcalase, Neutrase, Protex-40L, PTPF-1430, and KMFP-15) were tested at different concentrations (0.1, 0.2, and 0.3%, w/v) during hydrolysis at 55 for 4 hr. Hydrolysis with $^{\circ}C$ KMFP-15 showed the lowest pH by 7.3. The highest soluble solid ($24.3^{\circ}Brix$) and free amino acid (4,944 mg%) contents were obtained by hydrolysis with KMFP-15 (w/v) at 0.2% addition level, which was not significantly different from the sample hydrolyzed at 0.3% level. Under the optimal condition of KMFP-15 at 0.2%, porcine blood was hydrolyzed at 60 up to 8 hr. The $^{\circ}C$ free amino acid content reached the highest at 4 hr, and then decreased with longer hydrolysis time. Under the optimal hydrolysis conditions, porcine blood hydrolysis powder had plenty of crude proteins, amino acids, and minerals, including iron, potassium, and zinc. The results showed that porcine blood could be utilized as an useful source of food supplement. The optimum conditions of hydrolyzing porcine blood, using 0.2 KMFP at $60^{\circ}C$ for 4 hr, can be used in the commercial production of protein supplements, amino acid sources, and iron fortifying agents.

• The Journal of the Acoustical Society of Korea
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• v.12 no.1E
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• pp.82-88
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• 1993

다중 신호 분해 방법을 통계적 성능 분석하여 이 방법의 통계적인 성질을 몇가지 얻었다. 이 결과로부터 이제까지 알려진것보다 더 정확한 분해 문턱값을 얻었고 이를 써서 가까이 있는 두 신호에 대한 다중 신호 분해방법의 분해능력을 살펴 보았다.

• Culinary science and hospitality research
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• v.18 no.1
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• pp.15-26
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• 2012

The objective of this study is to optimize enzymatic hydrolysis of cooked krill by using Alcalase. To optimize krill hydrolysis on such dependent variables as TCA, DPPH-scavenging, and Fe-chelating activities by using Alcalase, independent variables of hydrolysis pH and temperature were investigated Their formulas and three dimensional graphs were obtained by using SAS and Maple softwares, respectively. For comparison of general composition of raw krill, its contents of moisture, crude protein, crude fat, and ash were 17.48%, 53.74%, 15.66%, and 10.21%, respectively, and for cooked krill, its contents were 4.80%, 71.84%, 5.26%, and 15.09%, respectively. The composition of fatty acids for cooked krill was similar to that of raw krill. The most abundant fatty acid was palmitic acid(16:0) and the following order was oleic acid(18:1), eicosapentaenoic acid (20:5), palmitoleic acid(16:1), and docosahexaenoic acid(22:6). For DH optimization of hydrolysates from cooked krill, its result was pH 8.5 and $66.6^{\circ}C$ hydrolysis temperature for the maximum DH of 29.4% For DPPH-antioxidative optimization of hydrolysates from raw krill, its maximum result of 27.1% was obtained in the hydrolysis condition of pH 7.4 and $67.5^{\circ}C$. For Fe-chelating optimization of hydrolysates from cooked krill, its maximum result of 24.9% was in the condition of pH 8.7 and $65.5^{\circ}C$. These results can be used for basic data for using krill products and other fish products as bioactive ingredients.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.465-469
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• 1998

The quantitative and rapid method for measuring the biodegradation of polymer materials in soil was developed. In this study, cellophane film was used as a model biodegradable polymer and the biodegradation was assayed by measuring the amount of glucose which was produced by a hydrolysis reaction using HCl after collecting the film from soil. Cellophane film was degraded 41.2% in 4 months during winter while it was degraded 76.5% in 2 months during summer. It means that biodegradation in soil is affected by environmental conditions. The biodegradation was also measured in an incubator (30$^{\circ}C$, humidity 50-55%) to exclude the environmental variations. Cellophane film was degraded 94% in that condition in 40 days. The biodegradation showed the first order kinetics and the rate constant was 0.067 (1/day). Acceleration of the biodegradation in soil was also studied. We added cultured soil microorganisms or nutrients such as N, P, and S into the soil. While the addition of microorganisms showed the temporary increase of rate constant, the addition of nutrients not only showed the increase of rate constant from 0.096 (1/day) to 0.21 (1/day) but also maintained the effect continuously.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.61-61
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• 1992

여러 점막 추출액증 Met-Enk의 효소적 분해는 매우 신속하였고 1차식에 따랐으며 점막측 추출액에서의 분해속도는 직장>눈>질>비강점막의 순이었다. 비강, 직장, 질점막의 점막측 및 장막측 추출액 간의 분해속도에는 유의성 있는 차이는 없었으나 눈점막의 경우 내피측 추출액에서의 분해속도 (1.03 $\times$ $10^{-3}$ $min^{-1}$)가 표피측 추출액에서보다 현저히 낮았다. 모든 추출액에서 검출된 주요 분해산물은 Tyr 및 Des-Tyr-Met-Enk로 천연 Met-Enk의 점막 추출액중 분해는 주로 aminopeptidase류가 관여되고 있음을 알았으며 Tyr-Gly, Tyr-Gly-Gly등의 분해산물이 소량 출현함으로 보아 endopeptidase류도 분해에 다소 관여하고 있음을 알았다. YAGFM은 Met-Enk보다 분해가 현저히 지연되었으며 그 분해속도도 직장>질>비점막의 순이었다. 이러한 분해는 분해산물로 Tyr이 검출되지 않은점으로 보아 YAGFM의 점막 추출액중 분해는 주로 endopeptidase류가 주로 관여되는 것으로 추정된다.

• Proceedings of the Korean Society for Language and Information Conference
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• 2008.06a
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• pp.125-137
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• 2008

한국어에서는 '보내-'가 여격 구문만을 허용하는 반면에, 영어의 send는 이중목적어 구문과 여격 구문 모두를 허용한다. 이런 비 대칭성은 어휘 해체 이론에 기반하여 볼 때, 영어에서는 send가 이중 목적어 구문에서 CAUSE와 전치사 $P_{HAVE}$로 분해되는 동사와 여격 구문에서 CAUSE와 $P_{LOC}$로 분해되는 동사가 존재하지만, 한국어에서는 '보내-'가 CAUSE와 $P_{LOC}$로만 분해되는 동사만 존재하기 때문에 나타나는 현상이다. 이는 한국어의 '보내-'가 '주-'와 결합할 때는 이중 목적어 구문을 허용하는 것으로부터 알 수 있다. '주-'는 $P_{HAVE}$를 갖는 동사와 $P_{LOC}$를 갖는 동사로 나누어지는데, $P_{HAVE}$로 나누어지는 '주-'가 '보내-'의 이중목적어 구문을 허용해 주는 것으로 본다. '주-'는 '보내-' 이외에도 사역 동사들과 수여 동사들의 이중 목적어 구문의 형성을 돕는다.

• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.237-240
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• 2006

Seventy-one marine bacteria decomposing sea tangle (Laminaria japonica) into single cell detritus (SCD) were isolated from sea water, sea tangle, sea mustard (Undaria pinnatifida), sea urchin (Anthocidaris crassispina), star fish (Acanthaster planci), and turban cell (Batillus cornutus), among which 14 strains decreased cutting strength of sea tangle and had alginate-degrading activity. Marine bacterium No. 34 isolated from turban cell showed lowest cutting strength of sea tangle, strongest alginate-degrading activity, and produced high content of $5-10\;{\mu}m$ SCD from sea tangle. This strain was identified as Vibrio sp. based on morphological, physiological, and biochemical characteristics and named as Vibrio sp. YKW-34.

• Journal of Life Science
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• v.22 no.12
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• pp.1724-1728
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• 2012

A marine bacterial strain that degraded fucoidan from Ecklonia cava was isolated from seawater. The crude fucoidanase of this strain efficiently degraded fucoidan at pH 8 and $50^{\circ}C$. The crude enzyme hydrolyzed 7.1% (w/w) fucoidan within 24 hrs from an 1% (w/v) fucoidan solution and produced oligosaccharides by endo-type hydrolysis as the reaction products. The results of 16S rRNA gene sequence analysis and biochemical tests permitted a tentative identification of strain SB 1493 as a Pseudoalteromonas species.

• Korean Journal of Food Science and Technology
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• v.27 no.6
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• pp.866-870
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• 1995

This study was conducted to determine the effect of soluble polysaccharides and indigestible polysaccharides on the digestibility of casein in vitro and the structure of polysaccharide. The digestibility of casein by trypsin in vitro was reduced to $80{\sim}89%$ and $69{\sim}99%$ by high molecular soluble polysaccharide (HMS-P) and low molecular insoluble polysaccharide (LMI-P) prepared from buckwheat, respectively. The digestibility of casein by chymotrypsin was reduced to $63{\sim}88%$ and$71{\sim}79%$ by HMS-P and LMI-P, respectively. But casein digestibiliy by trypsin and chymotrypsin was slightly reduced by LMS-P. The casein hydrolyzates inhibited by HMS-P gave a main peak in the void volume on Sepadex G-100, but the peak in the total volume was only appeared in case of LMS-P having no inhibito교 effect. It was suggested that the HMS-P was consisted of 4-linked and 4,6-dissubstituted glucose from the structural analysis by GC.