Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characteristics of Fucoidan Degrading Bacterium from Marine

해양으로부터 fucoidan 분해세균의 분리 및 특성

  • Lee, Yu-Ri (Department of Bio-Food Material, College of Medical & Life Sciences, Silla University) ;
  • Lim, Jong Min (Glucan Corp.) ;
  • Kim, Ki-Young (Glucan Corp.) ;
  • Mun, Sung-Bae (Glucan Corp.) ;
  • Kwak, Inseok (Department of Bioscience, College of Medical & Life Sciences, Silla University) ;
  • Sohn, Jae Hak (Department of Bio-Food Material, College of Medical & Life Sciences, Silla University)
  • 이유리 (신라대학교 의생명과학대 바이오식품소재학과) ;
  • 임종민 ((주)글루칸) ;
  • 김기영 ((주)글루칸) ;
  • 문성배 ((주)글루칸) ;
  • 곽인석 (신라대학교 의생명과학대 생물과학과) ;
  • 손재학 (신라대학교 의생명과학대 바이오식품소재학과)
  • Received : 2012.11.15
  • Accepted : 2012.12.21
  • Published : 2012.12.30
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Abstract

A marine bacterial strain that degraded fucoidan from Ecklonia cava was isolated from seawater. The crude fucoidanase of this strain efficiently degraded fucoidan at pH 8 and $50^{\circ}C$. The crude enzyme hydrolyzed 7.1% (w/w) fucoidan within 24 hrs from an 1% (w/v) fucoidan solution and produced oligosaccharides by endo-type hydrolysis as the reaction products. The results of 16S rRNA gene sequence analysis and biochemical tests permitted a tentative identification of strain SB 1493 as a Pseudoalteromonas species.

Ecklonia cava로부터 얻어진 fucoidan을 분해하는 해양세균은 해수로부터 분리하였다. 이 균주의 조효소는 pH8과 $50^{\circ}C$에서 fucoidan을 효율적으로 분해하였다. Crude fucoidanase는 1% (w/v) fucoidan 반응액에서 24시간 내에 약 7.1%를 가수분해하였으며 반응산물로서 endo-type 가수분해에 의한 oligosaccharide를 생산하였다. 16S rRNA 유전자 염기서열분석과 생화학적 시험의 결과로부터 SB 1493균주는 잠정적으로 Pseudoalteromonas sp.로 동정하였다.

Keywords

References

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