• Journal of Nutrition and Health
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• v.42 no.7
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• pp.605-614
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• 2009

Most cancer patients are treated with surgery, chemotherapy or radiation as anticancer therapies. Especially in the case of radiation, these treatments produce adverse effects such as vomiting, weight loss, anorexia, normal cell damage and malabsorption. The major goal of this study was to determine the effect of irradiation on the nutritional and immune status in irradiated rats. A secondary goal was to determine the effectiveness of high protein diet (HP) and resveratrol (Res) in minimizing the adverse effects of radiation. Rats were divided into four groups: normal diet (NP), HP, NP + Res and HP + Res groups. Each group was further divided into subgroups that received radiation (RT group) and one that did not (non-RT group). Each diet was supplied from $12^{th}$ day prior to irradiation treatment with irradiation dose of 17.5 Gy. The diets were continued until 10th day after radiation treatment and animals were sacrificed. The radiation treatment showed decreased body weight, serum protein and HDL levels and increased TG and LDL levels in nutritional status. HP, NP + Res and HP + Res groups reduced the level of serum LDL and TG in irradiated rats. NP + Res and HP + Res groups increased reduced albumin level of serum in RT group. In case of immune status, the radiation treat-ment showed decreased WBC, lymphocytes and increased neutrophil and eosinophil levels. The levels of serum IL-2 and IL-6 were significantly increased by radiation, however the cytokine levels decreased in all dietary treatment groups. These results showed that high protein diet and resveratrol supplementation seem to minimize the adverse effects of radiation on lipid nutritional status and inflammation response in the rat model.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.23-28
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• 2011

Resveratrol has been associated with reduced cardiovascular disease and reduced cancer risk. This phytochemical has been reported in a number of plant species including grapes, peanuts and pine trees in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to determine the resveratrol contents in leaves of mulberry varieties at different collecting times. Quantitative analysis of 16 cultivars showed a range of $102{\sim}466{\mu}g/100g$ on dry weight basis (which is equivalent to $25{\sim}116.5{\mu}g/100g$ on fresh weight basis). Resveratrol contents in mulberry leaves was higher in autumn than spring, and higher in fully matured leaves than in juvenile leaves. Among the tested samples, 'Kaeryangppong', 'Sugeppong' and 'Cheongilppong' collected in the middle of October showed high resveratrol contents of $838{\mu}g/100g$, $803{\mu}g/100g$, $800{\mu}g/100g$ on dry weight basis, respectively. Especially mulberry leaves dried in the shade showed of $1,030{\mu}g/100g$ on dry weight basis in resveratrol content, this result may contribute to utilization of mulberry leaves.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.3
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• pp.249-258
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• 2018

Airborne pollution causes oxidative damage, inflammation, and premature aging of skin. Resveratrol is a polyphenol compound that has various biological activities such as antioxidant, anti-inflammation, and anti-melanogenic activities but it is unstable to heat and light. Resveratryl triacetate (RTA) is a new cosmetic ingredient that is more stable than resveratrol and its skin safety and whitening efficacy have been reported previously. The purpose of this study was to examine the effects of resveratrol and resveratryl triacetate (RTA) on the inflammatory responses of human epidermal keratinocytes (HEKs) exposed to airborne particulate matters with a diameter of < $10{\mu}m$ (PM10). Cultured HEKs were exposed to PM10 in the absence or presence of resveratrol and RTA. Assays were undertaken to determine cell viability, the production of reactive oxygen species (ROS), and the expression of inflammatory cytokines. PM10 treatment decreased cell viability, and increased the expression of pro-inflammatory cytokines such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), $interleukin-1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6), and interleukin-8 (IL-8). Resveratrol and RTA reduced cell death and ROS production induced by PM10. PM10-induced mRNA expression of the inflammatory cytokines was either attenuated (IL-6), or enhanced ($IL-1{\beta}$), or unaffected ($TNF-{\alpha}$ and IL-8) by resveratrol and RTA. PM10-induced IL-6 protein expression was attenuated by resveratrol and RTA. This study suggests that resveratrol and RTA have activities regulating cell damage and inflammatory responses of the skin exposed to airborne particulate matters.

• Journal of the East Asian Society of Dietary Life
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• v.21 no.5
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• pp.739-745
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• 2011

This study was performed to investigate the enrichment of resveratrol content in harvested grapes using the modulation of cell metabolism with ultra-violet (UV) irradiation. Resveratrol, a phytoalexin, is produced by stilbene synthase (STSY) from malonyl-CoA and ${\rho}$-coumaroyl-CoA. Its biosynthesis has been reported to be induced by UV and other environmental factors. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that STSY Promoter 1 in grapes was very highly expressed by treatment with UV. Grapes were harvested and treated for post-harvest induction of STSY gene expression with UV, and then their resveratrol content was analyzed. UV treatment for 5 minutes provided the best condition for the induction of STSY gene expression. When harvested Gerbong and MBA grapes were treated with a prototype UV radiator, their resveratrol content was enriched upto 5 times compared with untreated grapes. These results suggest that a post-harvest UV treatment can be applied to enrich resveratrol content in grapes and add value to them.

• Journal of Life Science
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• v.32 no.1
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• pp.11-22
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• 2022

N-methyl-D-aspartate (NMDA) receptors have received considerable attention regarding their involvement in glutamate-induced neuronal excitotoxicity. Resveratrol has been shown to exhibit neuroprotective effects against this kind of overactivation, but the underlying cellular mechanisms are not yet clearly understood. In this study, HT-22 neuronal cells were treated with NMDA in Mg²⁺-free buffer and subsequently used as an experimental model of glutamate excitotoxicity to elucidate the mechanisms of resveratrol-induced neuroprotection. We found that NMDA treatment causes a drop in MTT reduction ability, disrupts inside-negative transmembrane potential of mitochondria, depletes cellular ATP levels, and stimulates intracellular ROS production. Double fluorescence imaging studies demonstrated an increased formation of mitochondrial permeability transition (MPT) pores accompanied by apoptotic cell death, while cobalt protoporphyrin and bilirubin showed protective effects against NMDA-induced mitochondrial injury. On the other hand, zinc protoporphyrin IX significantly attenuated the protective effects of resveratrol which was itself shown to enhance heme oxygenase-1 (HO-1) mRNA and protein expression levels. In cells transfected with HO-1 small interfering RNA, resveratrol failed to suppress the NMDA-induced effects on MTT reduction ability and MPT pore formation. The present study suggests that resveratrol may prevent mitochondrial injury in NMDA- treated HT-22 cells and that enhanced expression of HO-1 is involved in the underlying cellular mechanism.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.2
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• pp.267-272
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• 2017

Piceatannol (PIC) is a natural hydroxylated analog of resveratrol (RSV), which is a polyphenol known to extend lifespan by stimulating sirtuins. The aim of this study was to investigate the effects of PIC and RSV on the toll-like receptor 4 (TLR4)-nuclear factor kappa B ($NF-{\kappa}B$) pathway in mouse hepatocytes and an obese/diabetic KK/HlJ mouse model. AML12 mouse hepatocytes in the absence or presence of palmitic acids (PA) were treated with PIC ($50{\mu}M$) or RSV ($50{\mu}M$). Male KK/HlJ mice at 20 weeks of age were divided into three subgroups as follows: 1) obese and diabetic control (KK), 2) KK_PIC, and 3) KK_RSV. PIC and RSV were administered orally at a dose of 10 mg/kg/d for 4 weeks. Four weeks of PIC and RSV treatment did not affect body weight or food intake in KK mice. Serum fasting blood glucose was significantly reduced in KK_PIC, and 2 h oral glucose tolerance test area under the curve was significantly reduced by PIC and RSV treatment in KK mice. PIC tended to improve homeostasis model assessment of the insulin resistance index (HOMA-IR) and HOMA beta-cells in diabetic KK mice. TLR4 and $NF-{\kappa}B$ were down-regulated by PIC and RSV treatments in hepatocytes in the absence or presence of PA. Insulin receptor, AMP-activated protein kinase, peroxisome proliferator-activated receptor gamma, nucleotide oligomerization domain-like receptor family pyrin domain-containing 3, interleukin-1, and $NF-{\kappa}B$ were altered in PIC-treated livers. Collectively, PIC and RSV inhibited the $TLR4-NF-{\kappa}B$ pathway, and PIC seems to be more effective than RSV in the regulation of analyzed targets, which are involved in insulin signaling and inflammation in vivo.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.4
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• pp.567-571
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• 2005

In this study, the effects of varying the amount of water, soaking time at $25^{\circ}C$ and drying time after soaking at $45^{\circ}C$ on the induction of resveratrol were evaluated to optimize the soaking treatment by response surface methodology (RSM). After response surface regression (RSREG), the second-order polynomial equation was fitted to the experimental data. The analysis of variance showed that the model appeared to be adequate $(R^2=0.9547)$ with no significant lack of fit (p>0.1). From statistical analysis, amount of water and soaking time were found to be significant factors. On the other hand, drying time was not significant. Ridge analysis showed that the optimized parameters were $67.15\%$ for amount of water, 19.58 hr for soaking time, 65.56 hr for drying time. It was confirmed that resveratrol content was increased from $0.1\;{\mu}g/g$ to $4.55\;{\mu}g/g$ under the optimized conditions. In addition, the experimental values at the optimized condition agreed with values predicted by ridge analysis. The analytical method validation parameters such as accuracy, precision, and specificity were calculated to ensure the method's validity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.259-263
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• 2006

A direct HPLC method was used to analyze determination of resveratrol in grapes and wines. Content of resveratrol in different parts of 13 grape cultivars and 13 wines were quantified. The average total resveratrol content was $143.5{\mu}g/g$. Resveratrol contents in the skin, fruit, seed and fruit stem were distributed range of $0.9{\sim}104.8{\mu}g/g,\;0.1{\sim}0.5{\mu}g/g\;1.7{\sim}173.9{\mu}g/g\;2.9{\sim}247.4{\mu}g/g$, respectively. Resveratrol contents in 13 wines were distributed range of $253.3{\sim}11,271.6{\mu}g/g$.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.2
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• pp.105-112
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• 2015

Resveratrol is a natural polyphenol. It protects skin from skin injury, ultraviolet radiation and pathogenic attack. This study is to find the optimum condition for the preparation of ethosome loading amount of resveratrol in ethosome. Ethosomes were prepared by modified hydrated liquid crystal method. Investigation of factors affecting the entrapment efficiency and particle size of ethosomes was carried out. The particle size of ethosome were measured by particle analyzer. The loading efficiency of resveratrol in ethosome was measured by HPLC. The particle sizes were 111.2 ~ 112.8 nm and the loading efficiency of resveratrol was 81.25 ~ 88.75%. The optimum conditions for the preparation of ethosome was obtained from of lecithin : resveratrol : cholesterol : ethanol at a weight ratio of 2.0 : 0.08 : 0.05 : 20.0.

• Korean Journal of Food Science and Technology
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• v.50 no.1
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• pp.98-104
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• 2018

Oxyresveratrol (trans-2, 3', 4, 5'-tetrahydroxystilbene), found in many plants including grape, peanut and mulberry, is a phytoalexin, an antimicrobial and antioxidative substance that rapidly accumulates in areas infected by the pathogen. We examined the accumulation of oxyresveratrol in nine Morus alba L. cultivars with respect to storage time and temperature postharvest and infection with GRAS microorganisms. Among the nine cultivars, the Suwon cultivar showed the highest oxyresveratrol content (9.6-fold increase) postharvest, when stored at $30^{\circ}C$ for 7 days. The optimal temperature and postharvest storage time for oxyresveratrol accumulation was $30^{\circ}C$ and 6 days. When Ramulus mori was infected with five microorganisms, the accumulation of oxyresveratrol increased over 4-fold in response to B. coagulans infection. These results suggest that oxyresveratrol accumulation is influenced by storage temperature, storage time, Ramulus mori cultivars, and microbial attack. Therefore, postharvest storage for an appropriate time period at a suitable temperature might be a useful way to industrially produce Ramulus mori cultivars with high oxyresveratrol content.