• Title/Summary/Keyword: 동특성 예측

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Molecular Biological Characterization of Recombinant Baculovirus with an Expanded Host Range (숙주범위가 넓어진 유전자 재조합 핵다각체병 바이러스의 분자생물학적 특성)

  • 김우진;우수동
    • Journal of Sericultural and Entomological Science
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    • v.38 no.1
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    • pp.42-47
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    • 1996
  • To investigate the host range determining factors of nuclear polyhedrois virus (NPV), Autographa california NPV and Bombyx mori NPV were coinfected into the two different cell lines, BmN-4 and Sf-9. The recombinant baculoviruses, RecS-A6 and RecB-727 which have an expanded host range, were isolated from Sf-9 and BmN-4 cell lines, respectively. The molecular biological characteristics of the recombinant baculoviruses were investigated. The pathogenicity of RecB-727 was similar to that of wild type BmNPV, while the pathogenicity of RecS-A6 was relatively lower than that of wild type BmNPV. The restriction enzyme digestion patterns of parental viruses and recombinant viruses showed that the recombinant virus has an expanded host range by genetic recombination. Southern blot analysis revealed that the p10 gene of RecB-727 was derived from AcNPV genomic DNA, while RecS-A6 has p10 gene of BmNPV in a viral genome. To investigate the host range expansion mechanism of recombinant baculovirus, HindIII-SacI 0.6 kb DNA fragments of RecS-A6 and RecB-727 were cloned and sequenced. The results showed that of wild type BmNPV helicase gene, suggesting that the expanded host range of recombinant baculoviruses was due to the insertion of BmNPV helicase gene into AcNPV viral genome.

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Optimization of Steaming and Roasting Conditions for Maximized Hypoglycemic Properties of Polygonatum odoratum Tea (둥굴레차의 혈당강하 성분을 극대화시킬 수 있는 증자 및 볶음조건의 최적화)

  • Kim, Kyung-Tae;Kim, Jung-Ok;Lee, Gee-Dong;Kim, Jeong-Sook;Kwon, Joong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.4
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    • pp.549-556
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    • 2005
  • Response surface methodology (RSM) was applied in steaming and roasting processes of Polygonatum odoratum roots in order to monitor hypoglycemic components and sensory property. In steaming and roasting processes based on the central composite design with variations in steaming time $(60\~180\;min)$, roasting temperature $(110\~150^{\circ}C)$ and roasting time $(10\~50\;min)$, coefficients of determinations $(R^2)$ were 0.8691 (p<0.05), 0.8253 (p<0.l0), 0.8727 (p<0.05), 0.8706 (p<0.05) and 0.8316 (p<0.10) in soluble solid, stigmasterol, $\beta-sitosterol$, hypoglycemic component (total), and overall acceptability, respectively. The maximum value of soluble solid was $71.47\%$ in 65.24 min of steaming time, $126.93^{\circ}C$ of roasting temperature and 37.58 min of roasting time. The maximum value of hypoglycemic component (total) was $764.10\;{\mu}g/g$ in 107.76 min, $117.78^{\circ}C$ and 14.70 min. Meantime, the maximum value of overall acceptability was 6.89 in 126.04 min, $115.79^{\circ}C$ and 43.93 min. The predicted values in optimum conditions for hypoglycemic components and sensory property were in good agreement with experimental values.

Plant Community Structure Characteristic of the Evergreen Forest, Bijindo (비진도 상록활엽수림의 식물군집구조 특성)

  • Lee, Soo-Dong;Kim, Ji-Seok;Kim, Mi-Jeong;Kang, Hyun-Kyung
    • Korean Journal of Environment and Ecology
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    • v.30 no.2
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    • pp.228-242
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    • 2016
  • This research was conducted for understanding the evergreen forest community structure of the Bijin Island. The island was worth of vegetational value because of the representative warm temperate species such as Castanopsis cuspidata was distribute there. According to the results of community classification by TWINSPAN and DCA, the Bijin Island evergreen forest were classified by 9 communities, such as C. cuspidata, C. cuspidata-Pinus thunbergii, Neolitsea sericea-Camellia japonica, Platycarya strobilacea, Quercus serrata-Decidus broad-leaved, Alnus firma, P. thunbergii. Due to the C. cuspidata, N. sericea, Ca. japonica etc. were still dominated at canopy and under-canopy layer, so the evergreen forest will be sustain current conditions for a while if there is no artificial disturbances that were caused by human. Pl. strobilacea community will be expected to replace N. sericea and also Q. serrata-Decidus broad-leaved community will be changing caused by mutual competition. Normally, in the way of the succession process of the warm temperate forest, P. thunbergii will be expect via deciduous broad-leaved to evergreen forest such as Machilus thunbergii, C. cuspidata var. sieboldii, and N. sericea. In case of species diversity index, P. thunbergii comm.(1.2739), Q. serrata-Decidus broad-leaved comm.(1.2325), Decidus broad-leaved comm.(1.1807), and A. firma comm.(1.0854) are relatively high, while C. cuspidata and N. sericea-Ca. japonica that was dominated by evergreen species at canopy layer and P. thunbergii that was damaged by fire are relatively low(0.7380~0.8416). Soil pH was 4.72~6.33, electric conductance was 0.035~0.128dS/m, and content of organic matter was 3.4~17.4%.

Optimization for the Preparation Conditions of Instant Rice Gruel Using Oyster Mushroom and Brown Rice (느타리버섯과 현미를 이용한 즉석죽 제조조건의 최적화)

  • Lee, Gee-Dong;Kim, Hyun-Gu;Kim, Jin-Gu;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.29 no.4
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    • pp.737-744
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    • 1997
  • Four-dimensional response surface methodology was used for optimizing preparation conditions and monitoring sensory quality of instant rice gruel prepared using oyster mushroom and brown rice. Water absorption time of brown rice and glutinous rice to prepare instant rice gruel were 50 hr at $5^{\circ}C\;and\;1\;hr\;at\;20^{\circ}C$, respectively. The optimum conditions predicted for each corresponding sensory properties of instant rice gruel were 47.58% (rate of brown rice in water-absorbed brown and glutinous rice), 569.68 mL (content of solution) and 52.40 min (heating time at $120^{\circ}C$) in viscosity of instant rice gruel, 47.15%, 568.49 mL and 53.04 min in taste of instant rice gruel, 44.06%, 558.54 mL and 53.84 min in mouth-feel of instant rice gruel, and 46.20%, 561.64 mL and 51.60 min in overall acceptance of instant rice gruel, respectively. The optimum conditions, which satisfy all sensory properties of rice gruel, were 44%, 620 mL and 56 min in rate of brown rice in water-absorbed brown and glutinous rice, content of solution and heating time, respectively. Sensory scores predicted at the optimum conditions were in good agreement with experimental sensory scores.

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The Stability and Characteristic Analysis of Cut Slope Behavior using Real-time Monitoring System (상시 계측시스템을 이용한 붕괴 절토사면 거동 특성 분석 및 안정성 해석)

  • Baek, Yong;Koo, Ho-Bon;Jang, Ki-Tae;Yoo, Byung-Sun;Bae, Gyu-Jin
    • The Journal of Engineering Geology
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    • v.14 no.1
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    • pp.71-80
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    • 2004
  • The failure of cut slopes frequently occurs particularly during the thawing season and the rain season in summer. This study interpreted data collected from site to which a real-monitoring system was applied in order to analyze the causes of ground behaviors and to forecast future slope failure. As for research methods, this study analyzed the size and mechanism of failure by integrating the results of field surveys and measurements. Furthermore, it analyzed data transmitted by the monitoring system installed in the a result, three times of ground displacement occurred as well as a number of partial tension cracks. The cut slope composed of sandstone and siltstone started its initial behavior as a result of torrential downpour and the loss of support of the substructure. For quantitative analysis of the characteristics of ground behavior, this study measured 5 lateral lines. According to the result of the measurement, displacement happened little in the section to which countermeasure had been applied, but displacement of maximum 400mm happened in the section to which countermeasure had not bee applied. The analysis of data on displacement and rainfall suggested a close relationship between ground behavior and rainfall. According to the result of stability interpretation along with the change of ground saturation, stability rate appeared to be less than 1.0 when ground saturation is over 55%. Although the current trend of ground behavior is at a stable stage falling within the range of tolerance, it is considered necessary to continue monitoring and data analysis because ground displacement is highly possible with the change of temperature during the winter.

On-orbit Thermal Characteristic for Multilayered High Damping Yoke Structure Based on Superelastic Shape Memory Alloy for Passive Vibration Control of Solar Panels (태양전지판의 수동형 제진을 위한 초탄성 형상기억합금 기반 적층형 고댐핑 요크 구조의 궤도상 열적 특성 분석)

  • Min-Young Son;Jae-Hyeon Park;Bong-Geon Chae;Sung-Woo Park;Hyun-Ung Oh
    • Journal of Aerospace System Engineering
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    • v.18 no.1
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    • pp.1-10
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    • 2024
  • In a previous study, a structure of a superplastic yoke consisting of a thin FR4 layer laminated with viscoelastic tape on both sides of a shape memory alloy (SMA) was proposed to reduce residual vibration generated by a deployable solar panel during high motion of a satellite. Damping properties of viscoelastic tapes will change with temperature, which can directly affect vibration reduction performance of the yoke. To check damping performance of the yoke at different temperatures, free damping tests were performed under various temperature conditions to identify the temperature range where the damping performance was maximized. Based on above temperature test results, this paper predicts temperature of the yoke through orbital thermal analysis so that the yoke can have effective damping performance even if it is exposed to an orbital thermal environment. In addition, the thermal design method was described so that the yoke could have optimal vibration reduction performance.

Occurrence of Diatom in the Late Quaternary Sediments of the Northeastern East Sea (Sea of Japan) and its Paleoceanographic Changes (동해 북동부해역 제 4기 후기 퇴적물의 규조 산출과 고해양학적 변화)

  • Shin, Y.N.;Ikehara, K.;Yoon, H.I.;Kim, Y.;Woo, K.S.;Khim, B.K.
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.5 no.4
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    • pp.305-319
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    • 2000
  • A total of 50 diatom species and 1 subspecies belonging to 31 genera except Chaetoceros resting spores were identified in the 45 sediments subsampled from a gravity core GH98-1223 collected from the western Hokkaido Island located in the northeastern East Sea (Sea of Japan). The most dominant species is Thalassionema nitzschioides (Grunow) Hustedt, ranging 29 to 59% of the total assemblages, and most species including Denticulopsis seminae (Simonsen and Kanaya) Simonsen and Pseudoeunotia doliolus (Wallich) Grunow were less than 5% in average. Frequencies of cold-water species are generally higher than those of warm-water species and the vertical distribution of cold-water species was largely opposite to that of warm-water species in spite of ecological habitat difference. Frequency of cold-water species, D. seminae is reverse to that of P. doliolus, an indicator of the Tsushima Warm Current, which is consistent with diatom temperature value (T$_{d}$ value). The variation of T$_{d}$ values shows that the upper part of core with greater-than-average T$_{d}$ values represents postglacial warming trend. These T$_{d}$ values clearly demonstrate that the study area located in the northern part of the East Sea is gradually influenced by Tsushima Warm Current. In addition, the zig-zag variation in the lower part reflects the unstable seawater for diatom habitat. Chaetoceros resting spores indicating productivity and upwelling was 5.3 to 40%, with maximum peak at 80 cm. Chaetoceros resting spores/Chaetoceros vegetative cells, an indicator of relative amounts of biogenic material in the sediments was high at the upper 80 cm level, corresponding to the change of T$_{d}$ values. On the basis of diatom assemblages, the northeastern part of East Sea has experienced the effects of Tsushima Warm Current during the postglacial period of Holocene, which is similar to the modem climatic environment. However, the variation of P. doliolus reflects that the intensity of Tsushima Warm Current has been oscillated in the East Sea.

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Stand Structure and Dynamics in Forests around Nari Basin of Ulleung Island, Korea (울릉도 나리분지 주변 산림의 임분구조 및 동태)

  • Chung, Jae-Min;Hwang, Sun-Mi;Kim, Yoo-Mi;Shin, Jae-Kwon;Kim, Myong-Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.12 no.1
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    • pp.23-35
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    • 2010
  • For the sustainable conservation of forests within and around the somma of Nari basin in Ulleung Island, the stand structure and dynamics with the aspect, altitude and stand types were estimated by using analyses of importance value, plant species diversity, S$\o$rensen's similarity index and distributions of diameter classes. Results of the importance value analysis with vegetation stories on each stand showed that Fagus engleriana and Acer okamotoanum were dominant in most stands regardless of the aspect and altitude of the forest around the somma of Nari basin and the plant species composition among stands was not significantly different. The forest within Nari basin was composed of pine, beech, alder and deciduous-mixed stand. Moreover, the species composition was greatly different among the four stands. The diversity of plant species within stand was in general higher within Nari basin than around the somma, and among stands within Nari basin, the plant species diversity of pine and deciduous-mixed stand showed higher values than that of beech and alder stands. The similarity index among stands with aspect and altitude showed that the index among stands of eastern, western and northern slope in forest around the somma and forest within the basin ranged from 0.68 to 0.69, and the similarity among four stands with altitude was very high having index range from 0.85 to 0.95. The patterns of diameter class distribution of the major dominant species from four altitude classes followed an almost normal bell-shaped distribution. The results were interpreted as an indication that these forests will in general maintain a stable stand structure. Therefore, despite diverse topographic features around Nari basin, the stand structure and plant species composition among stands were not diversified as expected. For the conservation of diversity of forest plant species and of forest ecosystems, a promising and intensive program should be considered.

Computational Fluid Dynamics Model for Solar Thermal Storage Tanks with Helical Jacket Heater and Upper Spiral Coil Heater (상부 코일히터를 갖춘 나선재킷형 태양열 축열조의 성능예측을 위한 CFD 해석모델 개발 및 검증)

  • Baek, Seung Man;Zhong, Yiming;Nam, Jin Hyun;Chung, Jae Dong;Hong, Hiki
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.4
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    • pp.331-341
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    • 2013
  • In a solar domestic hot water (SDHW) system, solar energy is collected using collector panels, transferred to a circulating heat transfer fluid (brine), and eventually stored in a thermal storage tank (TST) as hot water. In this study, a computational fluid dynamics (CFD) model was developed to predict the solar thermal energy storage in a hybrid-type TST equipped with a helical jacket heater (mantle heat exchanger) and an immersed spiral coil heater. The helical jacket heater, which is the brine flow path attached to the side wall of a TST, has advantages including simple system design, low brine flow rate, and enhanced thermal stratification. In addition, the spiral coil heater further enhances the thermal performance and thermal stratification of the TST. The developed model was validated by the good agreement between the CFD results and the experimental results performed with the hybrid-type TST in SDHW settings.

Active Noise Control in Ductilike System using Adaptive Filtering (적응필터링에 의한 덕트계의 능동소음제어)

  • 이태연;김상명;송원식;오재응
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 1991.04a
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    • pp.17-22
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    • 1991
  • 최근 기계장치로부터 발생하는 소음을 감소시키는 새로운 방법으로서, 능동 적으로 소음을 제어하는 방법에 대한 연구가 활발히 진행되고 있다. 이것은 원하지 않는 소음을 그 신호의 역위상을 갖는 부가음을 이용하여 능동적으 로 감쇠시키는 방법으로서, 저주파수 대역에서 비효율적인 수동적인 방법인 소음기둥에 대한 대안으로 많은 학자들의 관심의 대상이 되어 왔다. 초기에 는 소음을 줄이기 위해 요구되는 여러가지 음향요소의 전달함수를 제어하는 데 대한 불가능성으로 인해 능동 소음제어에 대한 실질적인 발전이 지연되 어 왔으나 최근 마이크로 컴퓨터를 비롯한 전자공학의 발전으로 인해 적응 신호처리 분야가 등장하게 되었으며, 음향계의 소음을 원하는 수준까지 제어 하는 능동 소음제어의 실시간 구현이 가능하게 되었다. 그 중에서도 음이 1 차원적으로 전파한다고 볼 수 있는 길이가 긴 덕트구조물에서의 능동 소음 제어는 가장 기본적이며 현실적으로 자동차 배기계나 냉동.공조설비에 있어 서 실용적으로 적용할 수 있는 문제임 만큼 많은 연구가 이루어지고 있다. 이러한 능동 소음제어 방법을 음향계에 적용하였을 때, 부가적인 음을 발생 하는 제어용 스피커로 인해 입력마이크로폰으로의 음향궤환이 존재하고 이 에 따라 제어계가 불안정해질 수 있으며, 또한 변환기의 사용으로 인한 부가 적인 전달함수가 존재하므로 이에 대한 중요한 의미를 갖고 고려하여야 한 다. 본 연구에서는 적응 필터링 이론에 의한 소음원의 입력신호에 대한 최적 한 예측으로써 부가음을 발생시키고, 입력신호 및 제어된 출력신호간의 차인 오차를 최소화 시키도록 하는 오차적응제어법을 이용한 능동소음 제어 방법 을 제시하였다. 이와 아울러 제어계의 환경변화에 따른 파라메타의 변화에 적응적으로 응답이 가능해야 하는 적응 소음제어 시스템에서, 음향궤환과 함 께 필히 고려해야 하는 부가적인 전달함수의 영향을 고려한 능동 소음제어 에 대해 연구하였다. 경량화 추세에 따라 지반이나 케이싱이 경량이거나 유연하여 회전축과 동적으로 연성된 경우 회전축-베어링-지반으로 이루어진 2중구조의 회전축 계 동특성을 해석할 수 있는 프로그램을 개발하므로서 회전 기계류의 진동 전반에 걸친 문제점에 대한 그 원인과 현상을 명확히 분석하여 국내의 전기 계류의 보다 신뢰성있는 설계 및 제작자료를 확보하는데 기여할 수 있게 하 였다.존의 small molecular Gd-chelate에 비해 매우 큼을 알 수 있었다. MnPC는 간세포에 흡수된 후 담도계로 배출되는 간특이성 조영제임을 확인하였다. 장비 내에서 반복 시행한 평균값의 차이는 대체적으로 유의한 차이가 없었으나, 다른 장비에서 반복 시행한 장비간의 사이에는 유의한 차이가 있는 경우가 더 많았다. 따라서 , MRS 검사를 소뇌나 뇌교의 어떤 절환에 적용하기 전에 각 장비 마다 정상 기준치를 반드시 얻은 후에 이상여부를 판 정하는 것이 필수적이라고 생각된다.EX> 이상이 적절한 진단기준으로 생각되었다. $0.4{\;}\textrm{cm}^3$ 이상의 좌우 부피차를 보이는 모든 증례에서 육안적으로도 해마위축이 뚜렷이 나타났다. 결론 : MR영상을 이용한 해마의 부피측정은 해마경화증 환자의 진단에 있어 육안적인 MR 진단이 어려운 제한된 경우에만 실제적 도움을 줄 수 있는 보조적인 방법으로 생각된다.ofile whereas relaxivity at high field is not affected by τS. On the other hand, the change in τV does not affect low field profile but strongly in fluences on both inflection fie이 and the maximum relaxivity value. The results shows a fluences on both inflection field

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