• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.3
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• pp.193-200
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• 2004

This pot experiment was conducted to investigate the effects of systematic variation application of Fe, Mn, Cu, and Zn on forage performance of orchardgrass and white clover. The treatments of systematic variation were 0/100, 25/75, 50/50, 75/25, and $100/0\%$ in the Fe/Cu(trial-1), Mn/Zn(trial-2), and Fe+Cu/Mn+Zn(trial-3), respectively. The treatments of Fe/Mn/Cu/Zn(trial-4) were composed of $70\%$ in main element and $10\%$ in other 3 elements, respectively. 1. By the systematic variations of Fe, Mn, Cu, and Zn, the yields were more significantly influenced in white clover than in orchardgrass. In addition, the yields of white clover were closely correlated to the trends of root/nodule growth and flowering. In the Fe/Cu trial, the relatively high yields were obtained at the $100/0\%$ in orchardgrass and at the $75/25\%$ in white clover. The yields of white clover were more negatively influenced by the 100/0(Cu control) than by the 0/100(Fe control). The yields of orchardgrass, however, tended to be opposite to the above trends. 2. In the Mn/Zn trial, both forages showed generally high yields at the high ratios of Mn/Zn. Compared with orchardgrass, the yields of white clover were greatly decreased by the Mn-deficiency(low ratio of Mn/Zn). The effects of Zn on forage yields, however, were not recognized. 3. In the Fe+Cu/Mn+Zn trial, the yields of orchardgrass tended to be slightly different among the treatments. The yields of white clover, however, were relatively' high at the 75/25, and showed a severe decrease at the 100/0 in the 2nd half cuts. In the Fe/Mn/Cu/Zn trial, the yields of white clover tended to be relatively high at the Cu and Zn treatments. It was likely to be caused by the balanced Fe/Mn ratio.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.1
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• pp.43-48
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• 1993

This experiment was carried out to determine the effects of application time of cattle slurry on grazing pasture on the grass growth, dry matter yield, nutritive value, animal preference, and efficiency of pasture utilization. Four different application times of slurry(right now after grazing, 5th day, 10th day, and 15th day after grazing), and a no-slurry(control) plot were designed, 1991∼1992. Annual application amount of slurry was 40 ㎥/ha, and slurry was applicated in spring(2 times), summer and autumn. Total NPK fertilizer applicated were same as 280, 200 and 240 kg/ha in all treatment, respectively. The pasture was grazed with growing beef cattle(initial body wt.; ca. 250 kg) at every 30∼35 cm of plant height. The grass height, dry matter yield, crude protein yield, and other nutritive value were not different among treatments. However, the animal preference and efficiency of pasture utilization showed significant difference by application time of slurry. The highest grazing preference (1: the worst∼9: the best). and utilization efficiency (0∼100%) were observed by application time of right now after grazing (7.8, 76%), which was same to control(7.8, 77%), and followed by 5th day after grazing(7.3, 74%) and l0th day after grazing(6.9, 71%). But the preference and pasture utilization were greatly decreased at application time of cattle slurry of 15th day alter grazing(5.5, 60%). From the above results, the optimum application time of cattle slurry on grazing pasture to enhance animal preference, and efficiency of pasture utilization was within 5 days after grazing (less than 13.6 cm of plant height), and within 10 days after grazing (less than 18.5 cm of plant height) at least.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.307-313
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• 1997

Seed germination test done in laboratory does not coincide with field emergence in general. The experiments were carried out to examine the effect of priming and $GA_3$, treatment to seeds of Platycodon grandiflorum; Codonopsis lanceolata and C. pilosula on lapsed time to first seedling emergence, seedling emergence, morphological characters and growth and the cause of poor emergence of C. pilosula. No-treatment as Control (water), priming or $GA_3$ treatment was done with only distilled water for 2 days, $CA(NO_3)_2$ 150 mM for 2 days or $GA_3$ 0.1 mM for 3 days, respectively. Seedling emergence rate was counted every 2 days but morphological characters and dry weight of shoot and root were measured on 38 days after sowing. Their internal seed structures were examined with Scanning Electron Microscope. C. pilosula had poorer seedling emergence rate than P grandiflorum and C. lanceolata showing nearly same rate: Compared to the other treatment (s) P. grandiflorum displayed higher rate in priming and $GA_3$, treatments but C. lanceolata or C. pilosula did the greatest rate in only $GA_3$ or priming treatment, respectively. $GA_3$ treatment to seeds of P. grandiflorum and C. lanceolata shortened the lapsed time to seedling emergence in comparison with Control, 2-days water imbibition before sowing. In all the species plant height and number of leaves per seedling became shorter and less in priming treatment than the other treatments except plant height of C. Pilosula while their hypocotyl length was nearly same in all treatments. Although priming treatment had nearly similar effect to morphological characters, $GA_3$ treatment forced greater shoot, root and aftermath total dry weight per seedling. Poor seedling emergence of C. pilosula was caused by its seed defect like cleavage or lack of embryo, poor development of embryo and endosperm or their separation.

• Applied Biological Chemistry
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• v.19 no.3
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• pp.172-183
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• 1976

RNA, DNA and other phosphorus fractions were determined in the leaf and root of soybean plants different in phosphorus sensitivity grown in $NH_4-N,\;NO_3-N$ and urea medium. The phosphorus sensitive cultivars contained higher ASIP (acid soluble inorganic phosphorus) than the tolerant cultivars with all nitrogen sources. ASIP was highest in the urea treated plants and lowest in the nitrate treated plants. Total phosphorus content was mostly affected with increase in ASIP. When ASIP increased, acid solsuble organic phosphorus(ASOP), phospholipids (L-P), RNA-P, residual phosphorus (R-P) tended to increase, while DNA-P showed little change. The percent RNA-P or DNA-P of total phosphorus in the nitrate treated plant was twice that in the ammonium treated plant, which were also higher in tolerant cultivars regardless of nitrogen sources. The percent ASOP in total acid soluble phosphorus $(ASOP/ASP{\times}100)$ decreased as phosphorus sensitivity decreased. Indications are that phosphorus sensitivity depends on the relative sizes of phosphorus metabolic pools. Total dry matter yield was negatively correlated with total phosphorus (r=0.84 significant at 0.01P), ASIP (0.84 significant at 0.01P) and residual phosphorus (0.69 significant at 0.05P). ASOP showed positive correlation with L-P, RNA-P and DNA-P but negative with R-P. RNA-P was significanly correlated only with L-P (0.63 at P=0.01). There was significant interaction (0.01) among nitrogen sources, cultivars and phosphorus metabolic pools. Phosphorus sensitivity and ammonium toxicity appear to be same in view of energy metabolism, that is, the former inhibits the conversion of ATP to ADP (energy releasing) through phosphate potential while the latter inhibits ATP formation (energy storing).

• Journal of Bio-Environment Control
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• v.29 no.2
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• pp.171-180
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• 2020

The effect of three different light qualities on growth, photosynthesis, quality and safe parameters of hydroponic cultivated spinach (Spinacia oleracea L.) were investigated indoor. Three different light qualities were created of red (660 nm), blue (450 nm) and green (550 nm) LEDs corresponding at ratio R660/B450 = 4/1 (RBL); R660/B450/G550= 5/2/3 (WWL); R660/B450/G550 = 1/1/1 (WL), which were tested at the same intensity (PPFD =190 μmol m^-2 s^-1). The results showed that the plant height and leaf number were the lowest in WL treatment. The SPAD, Net photosynthesis rate Pn, Fv/Fm, Leaf area index LAI values and all parameters of root characteristics were the highest in RBL treatment and were significantly different from two others. Fresh weight of stem, leaf and root, dry weight of root in the three light qualities were significantly different. In contrast, the highest K⁺ content in WL was different from WWL and RBL treatments, while Ca²⁺ and Fe²⁺ content were the highest in the RBL treatment. Vitamin C content was significantly different between the three treatments. nitrate and oxalic acid contents were the highest in WL treatment, whereas soluble-solids contents and vitamin C contents were the highest in RBL treatment. Oxalic acid, nitrate contents were observed tending reduced under WWL although oxalic acid content in RBL treatment was not different from WL and WWL treatments. In all three different light treatments were not detected Salmonella, E.coli. Our results suggest that RBL may be appropriate light for growth of spinach, but supplementary green light to a combination of red and blue LEDs at the reasonable rate can change the quality of spinach in a positive direction. Hydroponic cultivated spinach was safe for users.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.4
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• pp.350-358
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• 1998

To determine the optimum application of fertilizers for the cultivation of tomato in plastic film house, eighteen soils which contained different salt contents were taken from four different areas under plastic film house cultivation, Youngdong, Boeun, Cheongweon county, and Cheongju city. The dry weight and the amount of N, P, and K uptakes of tomato in the plot with no fertilization were considered as the factors representing the fertility of the soil. The differences in the dry weight and in the amounts of N, P, and K uptakes of plants between the plots with fertilization and with no fertilization were considered as the factors representing the total effect of fertilizer and the effects of fertilizer N, P, and K, respectively. These factors of soil fertility and fertilizer effects were estimated by correlation and regression with the chemical properties of the soil in order to find the critical levels and recommended method for optimum fertilization of tomato. The standardized partial regression coefficients of inorganic nitrogen ($NO_3-N+NH_4-N$) contents in soil for the factors of fertility ranged from 247 to 1,159, showing the best, while those of the others ranged from 0.02 to 4.02. Those of inorganic nitrogen ($NO_3-N+NH_4-N$) contents in soil for the electrical conductivity were also the best and were ranged from 35.2 to 36.0 compared with the values of less than 1.0 of the others. These results demonstrate that the content of inorganic nitrogen in the soil is the best index for both soil fertility and electrical conductivity of the soil. The critical level of inorganic nitrogen ($NO_3-N+NH_4-N$) in the soil for maximum productivity with zero value of fertilizer effects for tomato, estimated through Cate-Nelson split method was $220mg\;kg^{-1}$. This was the same value as evaluation for the cultivation of chinese cabbage. In conclusion, for optimal application of fertilizer in plastic film house, 1) no fertilization is recommended when the contents of inorganic nitrogen in the soil is more than $220mg\;kg^{-1}$; however, 2) in the case of less than $220mg\;kg^{-1}$ of inorganic nitrogen content in the soil, the optimal level of fertilization could be estimated through the regression equation between fertilizer effects and content of inorganic nitrogen in the soil.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.3
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• pp.169-178
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• 2003

This pot experiment was conducted to find out the effects of boron application($B_{0}$ ; control, $B_1$; 0.2, $B_2$; 2.0, B$_3$; 6.0, $B_4$; 15.0 boron me/pot) on the forage performance of pure and mixed cultures of orchardgrass and white clover. The third part was concerned with the changes in the contents and yields of nitrogen compounds(crude/pure protein and soluble N-compound) in forages. The results obtained are summarized as follows: 1. With no additional fertilization, especially nitrogen, in a pure culture, the $B_{0}$ and $B_4$ treatments on white clover decreased the amount of crude/pure protein, and showed nitrogen deficiency symptoms. However, the optimum boron application($B_2$) positively resulted in the increase of crude and pure protein, especially pure protein, and the content ratio of pure protein/soluble N-compounds. With additional fertilization, especially nitrogen, differences were not found among the boron treatments($B_{0}$, $B_2$, and $B_4$). 2. Owing to the decline of white clover as affected by the additional fertilization, especially nitrogen, in the grass-clover mixed cultures, the effects of B-application on these contents of white clover were different and relatively low, compared with the pure cultures. But the positive effect of $B_2$ treatment tended to be similar to the pure cultures. Also, it was recognized that the $B_2$ treatment resulted in the increase of their contents in orchardgrass, however, the effect was relatively minor compared with that of white clover. 3. The optimum B application(B$_2$) on white clover influenced relatively better on the yields of crude and pure protein than on the dry matter yields, especially with no additional fertilization. The effects of boron application on the contents and yields of crude and pure protein were different according to the forage species, whether it was a pure or mixed culture, and additional fertilization.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.3
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• pp.159-168
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• 2003

This pot experiment was conducted in order to find out the effects of boron application($B_{0}$ /; control, $B_1$; 0.2, $B_2$; 2.0,$ B_3$; 6.0, $B_4$; 15.0 B me/pot) on the forage performance of pure and mixed cultures of orchardgrass and white clover. The 2nd part was concerned with the changes in the forage yields and concurrence index. The results obtained are summarized as follows: 1. The optimum boron application($B_2$) generally resulted in the increase of both forage yields, but the effects of boron application on them were different according to the forage species, whether it was a pure or mixed cultures, additional fertilization, and cutting order. The effects of boron application on the forage productivity were more obvious in white clover than in orchardgrass. 2. Owing to the decline of white clover as affected by the application of additional fertilizers, especially N, in the grass-clover mixed cultures, the effects of boron application on the white clover yields showed a numerical inferiority compared with the pure culture. It was recognized that the yield increase and botanical composition of white clover in grass-clover mixed cultures could be regulated by the application of additional fertilizers and boron. 3. The toxic boron application($B_3$ and $B_4$) resulted in a decreased yield of both forages. The yield change of orchardgrass tended to be similar between pure and mixed cultures, whereas it of white clover tended to be more negative in mixed than in pure cultures. 4. With the application of additional fertilizers, especially N, the botanical composition and concurrence index in grass-clover mixed cultures were relatively increased in orchardgrass, and decreased in white clover. The botanical composition of orchardgrass increased from 55% to 75%, whereas it of white clover decreased from 45% in the first half cutting to 25% in the second half cutting, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.1
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• pp.16-24
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• 1998

To determine the optimum application of fertilizers for the cultivation of Chinese cabbage in plastic film house, twenty soils which contain different salts contents were taken from 4 different area of plastic film house cultivation, Youngdong. Boeun county, Cheongweon county, and Cheongju city. The dry weight and the amount of N. P, and K uptakes of Chinese cabbage in the plot of no fertilization were considered as the factors representing the fertility of the soil. And a difference of dry weight and the amounts of N, P, and K uptakes of plants between the plot of fertilization and no fertilization were considered as the factors representing the total effect of fertilizer and fertilizer N, P, and K effects. respectively. These factors of soil fertility and fertilizer effects were estimated by correlation and regression with soil tests in order to find the critical levels and recommended method for optimum fertilization of Chinese cabbage. Chinese cabbage transplanted in two soils, having the electrical conductivity of 9.3 and 15.2 dS/m, were not able to root due to the salts toxicity. The content of inorganic N, the electrical conductivity, and CEC were founded to have significant correlation with the factors of both the soil fertility and fertilizer effects for the cultivation of Chinese cabbage. To determine the weighting degree for the productivity and the fertilizer effects, the standardized partial regression coefficient was analyzed by regression among the factors of fertility, the fertilizer effects, and the soil tests. The coefficient for inorganic N($NH_4-N$ and $NO_3-N$) was obtained as the absolute value of 756-1871 and this value was extremely higher than those of other soil tests which was 0.07-4.11. These results suggested that the content of inorganic N is the best tests for the estimation of the productivity and the fertilizer effects for the cultivation of Chinese cabbage in plastic film house. The critical level of inorganic N($NH_4-N+NO_3-N$) estimated by Cate-Nelson split method for maximum productivity and zero point of fertilizer effect was 220 mg/kg for all the factors of estimation. These results suggested that no application of fertilizer N. P, and K is required at the critical level of inorganic N of soil. Consequently the optimum application of fertilizer N, P, and K for the cultivation of Chinese cabbage in plastic film house was possible to determine by the critical level of inorganic N of soil. The critical level of electrical conductivity was estimated as 2.8 dS/m by the same method.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.858-863
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• 2010

The blueberry farming requires the soil condition of well-drainage, pH of 4.5 to 5.2, and high in organic matters for stable growth and development. Most of soil type of cultivated land in Korea, however, belongs to alkaline soils with low organic matter content and poor drainage. Therefore, the blueberry farmers use peat moss heavily to improve the soil condition, but the guideline on the effective and economic ratio of peat moss is not established yet. This study was performed to determine the cost effective peat moss ratio for amending soils, and to investigate the feasibility of using sawdust and coco peat as soil amendments. Peat moss, coco peat and sawdust are mixed with soil at the ratio of 0, 12.5, 50 and 100% (v/v). Among 3 organic materials with various mixture ratios, the pH of soil was the lowest in 100% peat moss and sawdust mixtures (pH 3.67 and pH 3.73, respectively), followed by pH 5.30 at 50% peat moss. The soil organic matter content are directly proportional to the mixture ratios in all three organic materials and the same trend was observed in the variation of content of exchangeable potassium in the coco peat treatments. On the contrary, the content of available phosphate, exchangeable calcium and magnesium decreased with increasing the ratio of organic materials. The nitrogen content in the leaves decreased as increasing the ratio of peat moss and coco peat in soil, but not of sawdust. The content of phosphate decreased but potassium increased as the ratio of sawdust and coco peat increased. There was no clear difference in the contents of magnesium and calcium among 3 organic materials. The plant height, stem diameter and dry weight of blueberry plants were the highest in 50 % peat moss, followed by 12.5% peat moss and 12.5% coco peat. The plants in 100% peat moss showed very poor growth. It can be concluded that peatmoss, when applied and managed appropriately, will be a good material for improving soil condition as well as securing desirable growth for blueberry. Upon coupling economic aspect, the optimum mixing ratio of peatmoss for blueberry farming is approximately 25-50%.