• Korean journal of applied entomology
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• v.58 no.4
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• pp.283-289
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• 2019

Overuse of antibiotics has significantly contributed to an increase in microbial antibiotic resistance, causing difficulties in the suppression of microbe-borne infectious diseases. In this study, we determined the anti-Klebsiella pneumoniae effect in the kidneys of mice induced by peptides isolated from H. illucens larvae. Mice were intranasally infected with a high dose of K. pneumoniae and 1 day later, peptides were introduced through the intramuscular route. Mice were sacrificed on day 10 upon K. pneumoniae infection to determine the bacterial loads in the kidneys. Mice receiving peptide treatment demonstrated significantly reduced bacterial loads, reduced bodyweight loss, and higher survival in a dose-dependent manner compared to control. These results indicate that peptide isolated from H. illucens larva inhibits K. pneumoniae infection in the kidney. The peptide from H. illucens larva could be a potential candidate for the development of an effective antibacterial drug.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.106-111
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• 2011

This study was conducted to investigate the effect of the mixture ratio of a soldier fly casts (SFC), compost and cocopeat on the soil physicochemical properties. The mixture ratios of soil amendment were 0%, 3%, 5%, 7% and 10% (V/V) incorporated with sand which met to the USGA particle standard. To analyze the effects of amendments on soil chemical properties, pH and EC were measured. The porosity, capillary porosity, air-filled porosity, bulk density and hydraulic conductivity also measured to analyze the physical properties. Chemical properties were significantly different by mixture ratios of a SFC, compost and cocopeat. Capillary porosity was a factor involved in soil physical properties by blending with a SFC and compost. It was affected on the volume of porosity or hydraulic conductivity. To analyze the correlation of mixture ratio versus to physical characters, the ratios of SFC were significantly different in capillary porosity, air-filled porosity, and hydraulic conductivity. These results indicated that mixing ratios of SFC were affected on soil physicochemical properties such as porosity and hydraulic conductivity of the root zone on the USGA sand green.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.298-305
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• 2013

Soldier fly casts produced by eco-friendly treatment of food waste with American soldier fly was composting cast mixed food waste compost and soldier fly pupa. This study was conducted to evaluate a possibility of soldier fly casts (SFC) as soil amendment by investigating soil properties and zoysiagrass growth. Amendments were selected coco peat, compost and SFC and their ratio mixed with sand were non-amendment (NA), 7% coco peat(Coco), 7% compost (Comp), 3.5% SFC (1/2SFC) and 7% SFC. In soil properties, SFC was increased CEC than NA and T-N, Av-$P_2O_5$ and exchangeable K in soil than Coco. Compared with Coco and Comp, turf quality such as turf color index and chlorophyll index in SFC was higher and root length of zoysiagrass longer. Growth of shoot, runner and root in SFC was increase than that of Coco and Comp. These results indicated that 7% SFC applied as soil amendment was improved growth and quality of zoysiagrass by prompting soil productivity and nutrient availability in soil and root growth.

• Journal of Life Science
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• v.30 no.4
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• pp.386-393
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• 2020

Insect industry has been focused as production of food, animal feed, pollinator and for environmental remediation. Hermetia illicens, called as black soldier fly (BSF) is famous as nutritive feed. In this study, to evaluate the antithrombotic activities of BSF, the larvae (instar 2~6), pupae, residue after adult emergence [RAAE] and adult of Hermetia illicens [black soldier fly, BSF] were collected and their ethanol extracts were prepared. Growth of BSF larvae was very rapid and the weight of larvae was increased to 25-folds during 10 days cultivation. The ethanol extraction ratios showed from 1.0% (pupae) to 18.5%(adults). The highest total polyphenol, total flavonoid, and total sugar contents were observed in RAEE (17.2 mg/g), pupae (3.4 mg/g), and instar 6 (37.6 mg/g), suggesting that metabolic changes occur during the life cycle of the BSF. Anti-coagulation assay showed that extracts of RAEE, instar 6 and pupa of BSF significantly inhibited thrombin, prothrombin, and blood coagulation factors. Furthermore, the extracts of RAEE, instar 3 and adult of BSF showed a strong platelet aggregation inhibitory activity. Our results suggest that pupae and RAEE of BSF have potential as antithrombotic agents. This is the first study to provide evidence of the antithrombotic activity of the BSF and bioactivity alterations during its life cycle.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.63-70
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• 2012

This study was conducted to investigated the distribution, ecological character and life cycle of Black Soldier Fly(BSF), Hermetia illucens, to determine effects of BSF on composing ability to the livestock feces. The distribution of BSF was defined in all parts of the country in Korea. Its main habitat was found to be areas near cattle sheds, feces sheds, living waste dump grounds, and food waste dump grounds. Excellent species of the bio-conversion using the livestock feces were selected out of two kinds of solider flies(Hermetia illucens and Ptecticus tenebrifer). And effects of BSF on composing ability to the livestock feces were pig feces 0.5 g, cattle feces 0.43 g and chicken feces 0.3 g per a larvae of BSF. In the examination of the ability of BSF to decompose food waste, volume of the livestock feces decreased by weight pig feces 77, cattle feces 83.62 and chicken feces 80%. Observed characteristics of BSF larvae and pupae stage were by developmental stage may be summarized as follows: size and length of larvae and pupae of BSF were higher than in pig feces and mixed sawdust treatments. In order to develop artificial indoor rearing techniques, The pupation and emergence rate were not visible the difference but important factor of the mating and egg-laying was a sun light. And it was visible the difference in mating ratio. Mating rate of H. illucens by seasonal change was the highest from May to July. Mating mostly occurred between 10 : 00 and 12 : 00 during which light intensity is high. As the egg-laying medium for artificial egg collection, calf feed and food waste were most effective. The ratio of egg-laying medium is waste food : sawdust(1 : 1) in volume. For egg-laying materials, flower foam and wood with holes were used; holes sized 3 ~ 5 mm in diameter, 7 ~ 10 mm in depth were most preferred for egg-laying.