• The Korean Journal of Zoology
• /
• v.6 no.1
• /
• pp.9-14
• /
• 1963

S.M.계인 마우스♂♀를 정상군과 4개의 실험군 즉 양성가속도(5G-50초간과 11G-50초간 매일 각각 폭로) 및 전격(5volt-2초간과 10 volt 2 초간 매일 각각 전격)군으로 나누어 최소한 22일간 순화시킨 후 여지 전지영동법에 의해서 Albumin/Globulin 바와 단백질분할을 측정하고 또한 혈청 전단백질, 혈색소량, Hematocrit 비, 적혈구포약성 및 간, 신장의 중량을 측정하였다. A/G 와 전단백질은 양성가속도의 G-force 증가와 전격적 증가의 각군이 정확군보다 유의하게 현저히 낮은 치를 나타내고 있다. 간장중량, 혈색소량 및 Hematocrit 비는 또한 정상군보다 양성가속도, 전격적 각 조건하에서 낮은 치를 나타내고 있다. 그러나 신장중량은 현저히 증가된 치를 나타낸다. 양성가속도와 전격적조건군의 간장의 조직학적 소견은 혈관적 변화가 나타나고 그리고 양성가속도에서는 또한 액포가 출현하였다. 적혈구 포약성은 거의 변화가 없었다. 이상의 결과로 미루어보아 상기 두 효과로 인해서 주로 간장기능 즉 단백질대사에 이상을 초래하는 것으로 생각된다.

• Proceedings of the Korean Journal of Food and Nutrition Conference
• /
• 2000.12a
• /
• pp.54.2-54
• /
• 2000

일반식용의 참쑥과 강화지역 특산물인 강화약쑥의 첨가급식이 체성장 및 성분에 미치는 영향을 관찰하고자 89g 내외의 흰쥐(Sprague Dawley) 24두를 3개 동물군(대조군, 참쑥군 및 약쑥군)으로 나누어 4주간 해당 실험식이(대조식이, 5%참쑥식이 및 5%약쑥식이)로 급식하였다. 쑥의 일반영양 성분 함량은 참쑥 중 조단백질과 조회분은 약쑥보다 약 2%이상 높았으나 반대로 조지방은 약 2%낮았다. 또한 약쑥 중 칼슘은 참쑥보다 5.3배 높았으나 반대로 참쑥 중 망간, 아연, 철, 마그네슘은 2.8∼2.3배, 비타민A는 2.9배가 높았다. 체중 증가율과 식이효율은 참쑥군 과 약쑥군이 모두 대조군과 비슷하였다. 혈청 중 총단백질, albumin, 요소질소, creatinine, 뇨산, 총cholesterol, HDL-C, LDL-C, 당 함량과 amylase와 transaminase(GOT, GPT)의 활성이 참쑥군과 약쑥군 간에 유의성 있는 차이를 나타내지는 않았으나 LDH의 활성은 약쑥군이 대조군과 참쑥군에 비하여 감소(p<0.05)하였다.

• Korean Journal of Acupuncture
• /
• v.19 no.1
• /
• pp.67-75
• /
• 2002

목적 : 자성(雌性) 흰쥐에서 phenylhydrazine으로 유발된 용혈성 빈혈에 오미자 약침의 항빈혈효과를 알아보기 위해서 적혈구수, hematocrit ratio, hemoglobin, 혈청 철함유량 및 serum total iron binding capacity (TIBC)를 관찰하였다. 방법 : 실험동물은 정상군, 대조군, 250 mg/kg 오미자 투여군 및 500 mg/kg 오미자 투여군으로 총 4개의 군으로 분류하였다. 정상군의 흰쥐는 아무런 처치도 하지 않았고, 대조군은 phenylhydrazine으로 용혈성 빈혈을 유발하였다. 오미자 투여군은 용혈성 빈혈을 유발한 후 7일간 매일 250 mg/kg 및 500 mg/kg의 용량으로 중완혈에 자침하였다. 결과 : 적혈구수, hematocrit ratio 및 hemoglobin은 대조군에서 정상군에 비해 감소한 반면 오미자 투여군은 증가하였다. 혈청 철함유량과 TIBC는 대조군에서 증가하였으나, 오미자 투여군에서는 감소하였다. 이러한 결과로 중완혈에 오미자 자침은 phenylhydrazine으로 유발된 용혈성 빈혈에서 항빈혈효과가 있는 것으로 사려된다.

• Animal Systematics, Evolution and Diversity
• /
• v.6 no.2
• /
• pp.165-172
• /
• 1990

The patterns of serum proteins in seven species of Korean rodents were analyzed by immunoprecipitin, immunodiffusion, and immunoelectrophoresis. It is found that the serum proteins of each species were different with one another and thatntigenic divergence among the seven species seems to be enormous , ie., serological correspondence ranged from 99.6 in Rattus norvegicus caraco(suborder Myomorpha) to 2.7 in Tamias sibircus asiaticus (suborder sciuromorpha). In the six species of the family Muridae (Suborder Myomorpha), the lowest value of 8.2 was shown in Clethrionmys rufocanus regulus (Subfamily Microtinae).

• The Korean Journal of Zoology
• /
• v.6 no.2
• /
• pp.1-5
• /
• 1963

SM계인 마우스 ♂♀를 정상군과 4개의 실험군, 즉 온열적 (33 $^{\circ}C$-60분, 매일 각각 폭로) 및 한냉적인 (5$^{\circ}C$-60분과 $0^{\circ}C$-60분, 매일 각각 폭로) 군으로 나누어 최소한 22일간 순화시킨 후 여지 전기영동에 의해서 Albumin /blobulin 비와 단백질 분할을 측정하고 또한 혈청전단백질량, 혈색소량, Hematocrit 비, 적혈구 포약성 및 간장 신장기관중량을 측정하였다. A/G 비와 전단백질양은 온열적 증가와 한랭적 증가의 각군이 정상군보다 유의하게 현저히 낮은 치를 나타내고 있다. 간장중량, 혈색소량 및 Hematocrit 비는 정상군보다 온열적 한랭적 조건하에서 낮은 치를 또한 나타내고 있다. 그러나 신장중량은 현저하게 증가된 치를 나타내고 있다. 온열적 한냉적 조건군의 간장에서의 조직학적 소견은 현저한 변화는 없고 다만 혈관적 변화만 나타내고 적혈구의 포약성은 거의 변화가 없다. 이와 같은 결과로 미루어 보다 온열적 한랭적의 효과로 인해서 주로 간장기능 즉 단백질대사에 이상을 초래하는 것으로 생각된다.

• Journal of Nutrition and Health
• /
• v.2 no.1
• /
• pp.51-54
• /
• 1969

Total amount of feed and calory intakes of mountain villagers show higher level than those of farming and fishing villagers, however, total amount of protein intakes of mountain villagers was much less than that of other villagers. Blood specific gravity, serum protein content and serum tryptophan content were low in inhabitants of mountainous area than those of other villagers, but in the serum protein fractions, the A/G ratio show higher value in inhabitants of mountainous area than those of other villagers. It is interesting result that serum protein tryptophan content presented a significant positive correlation with that of serum gamma globulin and a significant negative correlation with that of serum albumin. And the data obtained in the present study could be established by the result of animal experiment (rat experiment) reported by Koyanagi et al.

• The Korean Journal of Zoology
• /
• v.32 no.4
• /
• pp.429-440
• /
• 1989

Spirometru erinocei의 유충인 sparganum에서 추출한 조항원 단백질을 항원으로 하여 sparganosis, cysticercosis, hydatidosis환자의 IgG 항체와 정상인의 IgG 항체를 혈청반응시켜 ELISA와 EITB에 의해 교차반응을 일으키는 비 특이항원 성분과 종특이항원 성분을 추구하였다. 1. sparganum에서 0.01 M PBS (PH 7.4)로 추출한 조항원 단백질을 SDS-PAGE로 전개하여 290 Kd에서 23 Kd범위의 분자량을 가진 25개의 단백질이 분획되었다. 2. sparganum의 조항원을 항원으로하여 ELISA로 sparganosis, cysticercosis, hydatidosis 환자의 190 항체와의 혈청 반응치는 sparganosis환자에게서는 0.44 $\pm$ 0.07에서 1.90 $\pm$ 0.03으로 negative control normal sera의 0.D (0.15 $\pm$ 0.03)를 기준으로 하였을 때 모두 양성이며 민감도(sensitivity)가 100 %이었으며 cysticercosis, hydatidosis환자혈청에서 양성반응이 나타났으며 교차반응도 있었다. 3. EITB에서는 spargancsis환자의 IgG항체에 의해 16개의 항원성분이 인지되었으며 이 중 6개의 항원성분이 정상인의 혈청에서도 인지되어 교차반응을 일으키는 항원성분이었으며 cysticercosis환자혈청에서 인지된 4개의 항원성분 중 2개의 항원성분이 sparganosis 환자혈청에서 인지된 것과 같았으며 hydatidosis환자의 190항체에 의해 인지된 19개의 항원성분 중 12개의 항원성분이 sparganosis환자혈청에서 인지된 항원 성분과 같았다. 4. 290 Kd, 200 Kd, 28 Kd의 항원성분은 sparganosis환자의 196항체에서만 인지되었고 228 Kd, 152 Kd, 66 Kd항원성분은 hydatidosis환자의 19G항체에서만 인지되었으며 66 Kd항원성분은 sparganosis, cysticercosis, hydatidosis, 정상인의 혈청에서 모두 인지되었다. We studied the serological reaction between the specific and nonspecific antigenic components from metacestode (plerocercoid) of spiromeko erimacei and IgG antibodies in sparganosis, cysticercosis. hydatidosis patients and normal human sera by ELISA and EITB. We prepared the crude extracts of sparganlim from snake, Matrix tigrina laterolis and used as antigenic components. By SDS-PAGE, we detected a total 25 peptide bands (fractions) with 290 Kd to 23 Kd molecular weight, and 8 bands of these detected bands developed strongly by silver stain. In serological test, ELISA, we recognized the cross-reaction of antigenic components reacting with IgG antibodies in heterogenous sera, cysticercosis and hydatidosis patients sera. The crude antigenic components of sparganum showed the high sensitivity in sparganosis, hydatidosis patients sera, but showed lower sensitivity in cysticercosis patients sera than the sparasanosis, hydatidosis patients sera. Sixteen antigenic components of these 25 separated bands were recognized by antibodies In sparsanosis patients sera,8 antigenic components in normal human sera, 4 antigenic components in cysticercosis patients sera and 19 antigenic compoenents in hydatidosis patients sera. The crude antigenic compnenets with 290 Kd, 200 Kd, 125 Kd and 28 Kd molecular weight was only recognized in sparganosis patients sera, but 64 Kd antigenic component was nonspecific antigenic components which were also cross-reacted with sparganosis, hydatidosis, cysticercosis patients sera and normal human sera.

• Journal of Embryo Transfer
• /
• v.19 no.2
• /
• pp.133-145
• /
• 2004

Serum-contain is commoly used for the production of in vitro-derived bovine embryos. However, were biological activity of serum varies from lot to lot, time consuming to choose better serum with good quality and risks of virus, bacteria and mycoplasma infection. This study established serum-free culture systems of in vitro embryo development to efficiently obtain a large number of blastocysts from ovaries of Hanwoo and oocytes maturation, cell number, tlerance of cryopreservation. Secondly, serum-contain medium is suspected of contributing to the large calf size, dystocia, cersarean sections, calf mortality and confirmed these blastocysts are high quality in terms of cyotolerance, high rates of pregancy and normal birth. For these reasons, Culture media (IVMD101 and IVD101) designed specifically for the preimplantation bovine embryo are rather simplistic, being based on salt solutions with additional energy substrates and growth factors. An improved serum-free medium (IVMD101) was developed for bovine oocytes maturation in vitro. Proportions of embryos developing to the blastocyst stage cultured in both IVD101(32.4%) and IVD101(34.5%) serum-free media were higher than in TCM199+10% FBS(12.4%) serumcontaining medium. Futhermore, the cell numbers per blastosyst obtained in the serum-free media were superior to those of blastocysts developed in serum-supplemented medium. Also, cell numbers of blastocysts obtained in the serum-free media were similar with blastocysts derived in vivo. Survival rate blastocysts after 24 hr incubation after thawing, the blastocysts cultured in both IVD101(94.5%) and IVD101(95.8%) serum-free media were higher than in TCM199+10% FBS (52.5%) serum-containing medium. After 72 hr incubation after thawing, hatching rates of blastocysts developed in IVD101(78.4%) and IVMD101(83.7%) were sighnificantly higher than that developed in the serum-supplemented medium(32.0%). The pregnancy rates almost not different between fresh blastocysts(38.2%) and frozen blastocysts(34.9%). The results suggested that the improved serum-free media(IVMD101 and IVD101) offer several advantages over culture in serum-cotaining medium, including increased rates of blastocyst formation and high cel numbers. Additionally, the survival and hatching rates of embryos product in serum-free media after post-thaw culture were superior to those of embryos produced in the serum-containg medium and useful for the production of high quality bovine embryos for cryo-preservation. These improved serum-free media are beneficial not only for the study of the mechanisms of early embryogenesis but also for mass production of good quality embryos for embryo transfer, cloning and transgenesis.

• Food Science and Preservation
• /
• v.21 no.3
• /
• pp.412-420
• /
• 2014

This study was performed to investigate the effect of fermented Hovenia dulcis Thunb fruit water extract on biomarkers for acute (a) ethanol-induced hangover and chronic (c) ethanol-induced liver injury in rats. For acute ethanol-induced hangover, the rats were administered distilled water (D.W., 10 mL/kg body wt.), Hovenia dulcis Thunb fruit water extract (HWE, 400 mg/10 mL/kg body wt.) and fermented HWE (FHWE, 400 mg/10 mL/kg body wt.), respectively, before 40% ethanol (5 g/kg body wt.) was administered. For chronic ethanol-induced liver injury, the rats were randomly divided into the normal control (cNC), ethanol (cET), cET-HWE and cET-FHWE groups. The cNC and cET groups were administered D.W. (10 mL/kg body wt.) before 40% alcohol (5 g/kg body wt.) was administrered for 21 days. The cET-HWE and cET-FHWE groups were administered HWE (400 mg/10 mL/kg body wt.) and FHWE (400 mg/10 mL/kg body wt.), respectively before 40% ethanol (5 g/kg body wt.) administration for 21 days. For acute ethanol-induced hangover, the serum alcohol and acetaldehyde concentrations were more significantly reduced in the aHWE and aFHWE groups than in the aET group. Moreover, the effect of FHWE was greater than that of HWE. For chronic ethanol-induced liver injury, the serum ethanol, acetaldehyde, ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GTP) levels and the hepatic lipids concentration more significantly dropped in the cET-HWE and cET-FHWE groups than in the cET group. The FHWE administration showed faster recovery of the serum glucose concentration than in the cET and cET-HWE groups. The body weight reduction tended to normalize in the cET-HWE and cET-FHWE groups, which is ideal for chronic ethanol administration. These results suggest that FHWE has a protective effect against ethanol-induced liver damage, as evidenced by its ability to lower the serum ethanol and acetaldehyde concentrations after alcohol administration, and by its ability to decrease the level of ${\gamma}$-GTP and hepatic lipids. FHWE also elevated serum glucose concentration. Therefore, FHWE is effective in reducing ethanol-induced hangover and can play a beneficial role in the treatment of ethanol-induced liver damage as well as body weight reduction.

• Environmental Analysis Health and Toxicology
• /
• v.6 no.1_2
• /
• pp.71-82
• /
• 1991

Rat embryos were cultured out of the mother from the head-fold stages, 9.5 days to 11.5 days during which they start to develop the brain, eyes, ears and cardiovascular system etc. We principally did the basic experiment in order to establish the best condition of the rat whole embryo culture in our laboratory. The temperature in the culture system was maintained 37$^{\circ}C$$\pm$0.2$^{\circ}C$ for 48 hrs. The culture was carried out in humidified atmosphere of the air, intially, the bottles were equilibrated with 5% $O_2$,5% $CO_2$, and 90% $N_2$gas mixture. After 22 or 24 and 29 or 30 hrs the cultures were reequilibrated with 20% $O_2$, 5% $CO_2$,75% $N_2$and 40% $O_2$, 5% $CO_2$, 55%$N_2$respectively. Various types of sera were tested and for the purpose of minimizing the quantity of rat serum, artificial medium was also tried and it was determined that rat serum supported normal growth over a period of 48 hrs, based on total growth analysis and structural comparisons with in vivo specimens.