• Korean Journal of Environmental Agriculture
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• v.11 no.3
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• pp.235-242
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• 1992

Organic ligands in the environments are expected to play an important role in regulating the biotoxicity and fate of pesticides. Influences of dissolved humic and fulvic acids on the phytotoxicity of Paraquat were investigated using a bioassay with hydroponically grown rye as indicator species. Levels of Paraquat in water culture media were ranged from 0 to $12_{{\mu}M}$ and those of humic or fulvic acid were 1.0mM as a soluble carbon. Media were prepared in a factorial combination with pHs of 4.5, 6.5 and 8.5, Standard curves of necrosis days, fresh weight, and growth rates, as Phytotoxicity Indices, versus Paraquat concentrations were employed to evaluate the effects of organic ligands on phytotoxicity of paraquat. Organic ligand itself had little effect on rye growth, but Paraquat showed a high degree of toxicity. Paraquat started to show an intensive injury to rye at $0.4{\sim}0.6{{\mu}M}$ and upper critical phytotoxic concentration was estimated to be 11.0${{\mu}M}$ In the presence of organic ligands, times required to cause necrosis due to Paraquat were delayed upto 40%. Fresh weights and growth rates were upto 20% higher in treatments of organic ligands plus Paraquat than that of Paraquat alone. Results demonstrated that complexation of organic ligand with Paraquat reduced the bioabailability of Paraquat to rye.

• The Korean Journal of Pesticide Science
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• v.11 no.1
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• pp.52-58
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• 2007

ALA (5-aminolevulinic acid) has been proposed as a tetrapyrrole-dependent photodynamic herbicide and insecticide by the action of the protoporphyrinogen IX oxidase (Protox IX). The present study was conducted to determine growth responses of plant and insects to ALA, biodegradable biopesticidal substance. In the paddy condition experiment, plant height and shoot fresh weight of barnyardgrass (Echinochloa crus-galli) was more reduced by ALA than rice plants, even though both plant species show great phytotoxicity. Hairy crabgrass (Digitaria sanguinalis), a monocot weed, was more sensitive to ALA at 5mM under upland condition when ALA applied on the foliage, compared with soybean (Glycine max) as a dicot crop. ALA solutions were tested for their insecticidal and larvicidal activities against Spodaptera exigua (Hubner) and Tetranychus urticae Koch. by foliar application and leaf-dipping method. The result showed higher insecticidal activity of ALA at 10mM and its mixture with insecticide luferon against S. exigua. Strongest insecticidal activity against T. urticae was observed from the ALA solution at 10mM 72 days after application. This results show that ALA solution had potent herbicidal and insecticidal activities against agricultural pests even though their activities were lower than those of synthetic pesticides.

• Journal of Life Science
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• v.29 no.12
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• pp.1345-1350
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• 2019

Our recent study has revealed that in vivo intestinal transit rate (ITR) in normal mice was significantly increased by the administration of an aqueous extract of dried Gentiana scabra rhizomes and roots (GS-W) in a dose-dependent manner. Following on from our previous study, the effect of GS-W on ITR was measured in mice with experimentally induced gastrointestinal motility dysfunctions (GMDs) in the present study. GS-W showed no significant acute toxicity even at an oral dose of 5 g/kg to mice. ITR was significantly retarded in the GMD mice compared with that in normal mice, and this retardation was significantly recovered by the oral administration of GS-W in a dose-dependent manner. Furthermore, the ITR value of GS-W at a dose of 1 g/kg appeared to be higher than that of cisapride, which was predominantly prescribed for human patients with various GMDs in the late 1900s but was withdrawn from the market in 2000 due to its fatal side effects. The current results suggest that GS-W is a potential substitute for cisapride to prevent or alleviate human GMDs.

• Food Science of Animal Resources
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• v.23 no.2
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• pp.186-192
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• 2003

The health benefits of friendly bacteria first came to the attention of the general public in 1908, when Dr. Elie Metchnikoff, a Russian biologist, wrote The Prolongation of Life. The longevity may be, in part, due to the antioxidative ability of lactic acid bacteria. However, the antioxidative effect of lactic acid bacteria has been reported only recently. Many kinds of reactive oxygen species can be formed in the human body and in food system, oxidative stress plays a significant pathological role in human disease. Antioxidants are effective for the reduction of oxidation induced by oxygen radicals by scavenging reactive oxygen species. Various synthetic and natural antioxidants have been reported, but there are doubts about the safety and long term effects on health. Antioxidants from natural sources are likely to be found more desirable. An elevated scavenging ability of reactive oxygen species would be a good property for commercially applied lactic acid bacteria. Antioxidant supplement or food containing antioxidants would be greatly applied for the reduction of oxidative damage for human body, and lactic acid bacteria are potentiated candidates for the production of functional foods or natural antioxidant supplements.

• Korean Journal of Weed Science
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• v.15 no.3
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• pp.206-213
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• 1995

Glyphosate(N-[phosphonomethyl]glycine) applied to the assimilate-exporting leaves(i.e. third old leaf) of tomato(Lycopersicon esculentum Mil var. Moneymaker). Herbicide binding protein, QB protein(D1), has been immunoblotted using the antibodies raised against the hybrid-protein expressed by a part of spinach psb A gene cloned in frame with the 3'end of lac Z gene to allow expression of the ${\beta}$-galactosidase(EC 3.21.23) in Escherichia coli. Glyphosate has an effect on a turnover of D1 within photosystem II of thylakoid membrane. The dysfunction of D1 protein within light harvesting complex(LHC-II) seems to be a pleiotropic effect of glyphosate.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.4-4
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• 2010

식물자원은 예로부터 인류의 생활과 긴밀한 관계를 유지하면서 인류에게 삶의 터전을 제공하였으며 쾌적한 환경을 유지시켜 주는 기반이 되었음은 물론 인류의 생존을 위한 많은 재화를 공급하여 왔다. 이러한 식물자원의 가치는 인간생활의 기초적 의 식 주 뿐 만 아니라 질병치료를 위한 재료로도 활용되고 있다. 제주도는 지리적으로 아열대권 생물자원의 확보 및 관리가 용이한 위치에 있어 아열대 자원 및 동북아섬 자원의 보존과 활용을 위한 전진기지로서 최적지이며, 난대, 온대, 한대 및 아고산대에 걸쳐 2,100여종의 식물자원이 분포하는 생태계 보고로 알려져 있다. 특히, 진시황이 불로초를 찾아 신하를 보낸 곳이 제주도라고 전해지고 있는 만큼 이는 제주가 많은 자원식물이 분포하고 있음이 그 옛날에도 알려져 있었다는 증거라 할 수 있다. 제주에 분포하거나 재배되는 식물 중 약용 및 식용 식물자원으로 잠재적 가치를 갖고 있는 식물은 550여 분류군으로서, 양치식물 43종류, 나자식물 6종류, 단자엽식물 31종류, 쌍자엽식물 370종류 등이다. 이러한 자원식물 중 상록성 목본 31종류, 낙엽수 105종류, 만경류가 51종류, 초본류(1년초 또는 다년초)가 358종류이며, 뱀톱, 천남성, 박새 등 독성을 가지는 식물도 43종류가 포함되어 있다. 비록 기존 알려진 약용 및 식용 식물자원이 산업적 중요성을 가지고 있다고는 하나 그 약효나 효능의 검정이 제대로 이루어지고 있지 않은 나머지 자생식물까지 포함하면 상당수의 제주 자생식물이 자원식물로 사용될 수 있는 잠재적 가치를 가지고 있다고 할 수 있다. 최근 생활수준의 향상으로 삶의 질과 건강에 대한 관심이 집중하고 있으며, 자원식물 유래 천연물을 소재로 한 향장품 및 건강기능성 식 의약품을 포함한 기능성 상품은 안전성이 보장되며 우수한 생리활성이 있는 것으로 밝혀지면서, 자원식물을 소재로 한 기능성 상품의 개발에 대한 수요가 증가하고 있는 실정이다. 제주지역도 청정지역 제주에 자생하고 있는 자원식물의 잠재적 가치를 활용하여 미래 제주 창출을 위한 신산업육성을 위한 노력을 기울이고 있다. 특히, 제주 자원식물들의 잠재적 가치를 활용하여 부가가치를 높이기 위해 건강 뷰티 생물산업을 지역전략산업으로 추진하면서 제주 자원식물들의 새로운 잠재적 가치를 조명하고 있어 이에 대한 지역내 추진노력 및 관련 산업현황 등을 정리하여 몇가지 견해를 제기하고자 한다.

• Korean journal of applied entomology
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• v.43 no.1
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• pp.35-41
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• 2004

Bacillus thuringiensis produces crystal proteins toxic to medically and agriculturally important pests during sporulation. To improve the activity of insecticidal crystal protein in applying to mosquito larval control, an expression vector, pSyn4D harboring the mosquitocidal cry11Aa gene under control of psbA promoter of Amaranthus hybridus was constructed. This expression vector was transformed into Synechocystis PCC6803 and a transformant, Tr2C was selected with kanamycin. The mosquitocidal cry11Aa gene was stably integrated Into genomic DNA of Tr2C in PCR detection using cry11Aa-specific primers. The transformant expressed 72-kDa Cry11Aa protein and median lethal time (LT$\sub$50/) was approximately 2.1 days for Culex tritaeniorhynchus larvae and 0.7 day for Anopheles sinensis larvae, respectively. These results suggest this transformant can be used for mosquito larval control as a biological control agent.

• Journal of the Korean Data and Information Science Society
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• v.27 no.4
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• pp.1001-1012
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• 2016

It is well known that air pollutants exert a bad influence on human health. According to the United Nations Environment Program, 4.3 million people die from carbon monoxide and particulate matter annually from all over the world. Carbon monoxide is a toxic gas that is the most dangerous of the gas consisting of carbon and oxygen. In this paper, we used 1 hour, 6 hours, 12 hours, and 24 hours average carbon monoxide concentration data collected between 2004 and 2013 in Daegu Gyeongbuk area. Parameters of the generalized extreme value distribution were estimated by maximum likelihood estimation and L-moments estimation. An evalution of goodness of fitness also was performed. Since the number of samples were small, L-moment estimation turned out to be suitable for parameter estimation. We also calculated 5 year, 10 year, 20 year, and 40 year return level.

• Environmental Analysis Health and Toxicology
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• v.22 no.3
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• pp.211-218
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• 2007

Even though some standard developmental bioassay protocols for environmental assessment using sea urchins have already been described, there have not been many attempts to apply and modify these protocols with Korean species. Therefore, there is a strong need to establish standard bioassay protocols using sea urchins commonly found in Korea. Prior to developing a new protocol, it is essential to know the optimal conditions for the bioassay procedures. We investigated the optimal conditions (temperature, salinity, and embryo density) of the sea urchin Strongylocentrotus intermedius. The ideal temperature for developmental bioassay of S. intermedius was determined to be $15^{\circ}C$ and the time required for the embryo to become pluteus larva was 72 hr. The optimal range of salinity for the embryo toxicity using S. intermedius was between 30 to 32 psu, which is similar to the range found in the natural habitats of adult populations. The optimum density of embryos at the beginning of bioassays was 100 embryos/mL. When the assays were carried out at higher densities, the proportion of normally developed larvae decreased significantly.

• Journal of Preventive Medicine and Public Health
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• v.30 no.3 s.58
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• pp.577-584
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• 1997

Validation and Standardization of neurobehavioral instrument in Korean occupational setting has not been studied ever. This study tried to validate the newly developed computerized psychomotor tests, Neurobehavioral Tests for Occupational Screening (NTOS). Male patients with Parkinson's disease(n=12) and male workers who never exposed to occupational neurotoxic materials and didn't have neurologic disease(n=21), performed some tests from NTOS; simple reaction time, choice reaction time(2 choice), and finger tapping(both hands). In simple analysis, difference between patient group and worker group was significantly great. Adjusted for age and education years, simple reaction time and finger tapping(both hand) were statistically significantly different between two groups(p<.05). Choice reaction time was also different(p<.1) but error frequency of choice reaction time test was not. Generally, this results showed NTOS could detect impairment of psychomotor function. But insensitive results of choice reaction time was partly due to small sample size and confounding variables and so required future study and refinement at improvement of NTOS.