• Title/Summary/Keyword: 더뎅이병원균

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Characteristics of Potato Common Scab Pathogens from Continuous Cropping Fields in Korea (국내감자 연작지대에서 분리한 더뎅이병원균의 특성)

  • 김주희;이왕휴
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.109-115
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    • 1996
  • 국내 감자 연작 재배지에서 수집하여 분리동정한 더뎅이병원균인 Streptomyces scabies의 배양적, 형태적, 생리적 특성을 조사한 결과는 다음과 같다. 이병감자에서 분리된 균들은 병원성 균주와 비병원성 균주들로 구분되었고, 이들 간에는 뚜렷한 차이를 보였는데, 전형적인 병징을 나타내는 병원성균주는 비병원성균주와는 달리, 나선형의 포자사슬, 회색 포자, 멜라닌 색소를 생성하고 D-glucose, L-arabinose, D-fructose, D0mannitol, raffinose, rhamnose, sucrose, i-inositol, D-xylose 등의 탄소원을 이용하였으며, 또한 7% NaCl 및 streptomycin sulfate, crystal violet, olean domycin(10$\mu\textrm{g}$/ml, 100$\mu\textrm{g}$/ml)등의 항생물질에 감수성을 나타내었다.

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Screening of Antagonistic Actinomycetes for Potato Scab Control and Isolation of Antibiotic Compound (감자 더뎅이병원균에 대해 길항활성을 갖는 방선균 탐색 및 항균 활성물질의 분리)

  • Lee, Hyang-Burm;Cho, Jong-Wun;Lim, Chi-Hwan;Kim, Chang-Jin
    • Applied Biological Chemistry
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    • v.47 no.2
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    • pp.164-169
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    • 2004
  • In the course of our screening for biocontrol agent (BCA) against Streptomyces scabiei and S. turgidiscabies causing potato scab using 5,000 actinomtcete isolates, 9 antagonistic strains were selected as BCA candidates through in vitro and in vivo assay. An antagonistic strain, A020645 was highly resistant to some pesticides and antibiotics such as dazomet and mancozeb and showed high control value in vivo. Two bioactive compounds (compound A, B) were purified by anion exchange chromatography, solid phase (ODS) extraction, TLC and reverse phase HPLC. Their chemical structures are now thought to be nucleoside derivative as determined by $^1H-NMR$ data analysis. Their full chemical structures would be elucidated through $^{13}C-NMR$, HMQC and HMBC analyses. Further studies will be focused on fitness in soil and formulation of the BCA candidates.

Phylogenetic Differentiation of Streptomyces spp. Isolated from Potato Scab Lesions in Jeju Island of Korea on the Basis of 16S rRNA Gene Sequences (제주도 감자 더뎅이병징에서 분리된 Streptomyces spp.의 16S rRNA 유전자 염기서열 분석)

  • Lee, Soo-Hyun;Ko, Young-Hwan;Kim, Chang-Jin;Kim, Bum-Joon;Lee, Keun-Hwa
    • Microbiology and Biotechnology Letters
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    • v.35 no.4
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    • pp.347-351
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    • 2007
  • Potato scab is prevalent in all potato-growing areas of Jeju Island and causes economically significant losses. Streptomyces species are known as pathogens of potato scab. In this study, we analyzed the 16S rRNA sequences of Streptomyces spp, which are isolated from potato scab lesions in Jeju Island, and constructed 16S rRNA phylogenetic tree. All isolates were clearly differentiated into the genus Streptomyces, and the tree also showed that new scab-causing Streptomyces spp or not yet named species of Streptomyces are existed in Jeju Island, Korea.

Development Characteristics of Potato Common Scab in Jeju Inland (제주지역 감자더뎅이병 발생 특성)

  • Hong, Soon-Yeong;Kang, Yong-Kil;Hahm, Young-Il
    • Research in Plant Disease
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    • v.9 no.3
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    • pp.137-144
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    • 2003
  • This study was conducted to clarify developmental characteristics of scab in potato fields in Jeju island, Korea from 1995 to 1999. Occurrence of potato scab increased with repeated cultivaton of potato and high soil pH in the fields. Incidence of the disease was high as 54.8% in the repeatedly cultivated potato fields but relatively low as 20.8~26.3% in the non-cultivated fields and in the fields where barley and Chinese cabbage were formerly cultivated. A total of 66 isolates were obtained from the diseased patato tubers and identified as Streptomyces scabies, S.turgidiscabies and S. acidiscabies. The isolation frequency of each Streptomyces species was 37.7%, 14.8% and 18.0%, respectively. The optimum temperature for mycelial growth of the Streptomyces spp. was $28~30^{\circ}C$, and the optimum pH for that 6~7.

Study on Medium Ingredient Composition for Enhancing Biomass Productionand Anti-potato Common Scab Activity of Streptomyces sp. A020645 as a BCA Candidate (생물제제(BCA) 후보균주인 Streptomyces sp. A020645 의 대량 균체생산 및 항더뎅이병 활성증진을 위한 고체배지 조성에 관한 연구)

  • Lee, Hyang-Burm;Roh, Hyo-Young;Park, Dong-Jin;Lee, So-Keum;Ko, Young-wan;Koh, Jeong-Sam;Kim, Chang-Jin
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.66-71
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    • 2005
  • The effect of medium components such as wheat bran, rice bran, oat meal, and soybean meal as basic ingredients and KH2PO4, glucose, and molasses as additives on mass production and anti-potato common scab activ ity of a streptomycete A020645 strain as a biocontrol agent (BCA) candidate was investigated. Of basicingredients, oat meal was the best one for mass poduction and enhancement of anti-potato common scabactivity. The biomass production of the active strain was more enhanced when 0.1-0.01.% glucose or molassesas additive were added into the basic medium. These information may have important implications in applying for effective formulation of BCA.