Intracellular calcium concentration ($[Ca^{2+}]_i$) may play a crucial role in a variety of neuronal functions. Here we report that in primary culture of mouse cerebellar granule cells nicotinic acetylcholine receptors (nAChRs) are expressed in a specific developmental stage and involved in the regulation of intracellular calcium homeostasis. Nicotine-mediated calcium responses were measured using $^{45}Ca^{2+}$ or fluorometrically using the calcium-sensitive fluorescent dye fura-2. Maximal uptake of $^{45}Ca^{2+}$ evoked by nicotine in mouse cerebellar granule cells were revealed $8{\sim}12$ days in culture. In contrast, nicotine did not alter the basal $^{45}Ca^{2+}$ uptake in cultured glial cells. In cerebellar granule cells nicotine-evoked $^{45}Ca^{2+}$ uptake was largely blocked by the NMDA receptor antagonists. Glutamate pyruvate transaminase (GPT). which removes endogenous glutamate, also prevented nicotine effects, implying the indirect involvement of glutamate in nicotine-mediated calcium responses. Fluorometric studies using fura-2 showed two phases of nicotine-evoked $[Ca^{2+}]_i$ rises: the initial rising phase and the later plateau phase. Interestingly, the NMDA receptor antagonists and GPT appeared to inhibit only the later plateau phase of nicotine-evoked $[Ca^{2+}]_i$ rises. The present results imply that nicotine mediated $^{45}Ca^{2+}$ uptake and $[Ca^{2+}]_i$ rises are attributed to the calcium fluxes through both nAchRs and NMDA receptors in a time-dependent manner. Consequently, nAChRs may play an important role in neuronal development by being expressed in a specific developmental stage and regulating the intracellular calcium homeostasis.
Journal of the Korean association of regional geographers
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v.2
no.2
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pp.219-231
/
1996
The aim here is to analyse the system of description of GIS in all of the high school textbooks passed with the official approval, to find the degree to which teachers understand about GIS, and to consider the present condition of GIS instruction. Most of the authors of textbooks generally underestimate importance of GIS, and there is difference among their awareness. In the system of description of GIS, there are only a few kinds of textbooks in which explanation of GIS is made coherently from the purpose of instruction aim through the chapter summary and to overall test in both of the Korean Geography and the World Geography. This trend is due to the degree of distribution of the GIS specialists in writing a textbook while the other texts books shows just a brief introduction of GIS concept. Although there is the limit for teachers to study how to teach GIS due to its very technological aspect as well as few previous training and teacher's guide. Thus it is evident that about a half of teachers who responded taught high school students without a knowledge on GIS, and a few of them even never referred to that concept. These facts may negatively affect the status of a geography in the society of information. For the solution of these issues, it is considered how to repair the description system and its contents. Besides, the variation among textbooks is reduced at the further revision of the 7th curriculum. And the printed matters of GIS are sufficiently provided for the teachers to use as their teaching aids. It is desirable that the GIS instruction models should be further developed for college education, and the programs for the on-the-job teachers training should be arranged. Besides, the previous training for the on-the-job teachers should be achieved more practically with enough time before the revision of curriculum.
Journal of The Korean Society of Grassland and Forage Science
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v.19
no.3
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pp.251-258
/
1999
A field experiment was carried out to determine the effects of chemical /mechanical treatments at mowing on the field drying rate and hay quality of rye (Secale cereale L.). The chemical drying agent/mower conditioner ($K_2CO_3$ 2%, conditioning, $K_2CO_3$ 2% + conditioning and control) were treated at different harvest stage (late boot, heading and bloom stage) for hastening hay-making in the spring of 1996. After field dry, square bales were made by hay baler, and the dry matter(DM) loss, visual estimation and nutritive value of rye hay were evaluated after storing two months. The field drying rate of rye was higher with delayed stage of harvest, and mechanical and chemical + mechanical treatment, but the effectiveness of chemical alone was very low. With mower conditioning, the duration of field dry was shortened by 1.5 to 2 days compared with control. The DM loss of rye hay was reduced by late harvest and mechanical, and chemical + mechanical combined treatment, but the efficiency by chemical alone was very low. The visual score (Ieafiness, green color, odor and softness) of hay after storing was high in mechanical and chemical + mechanical, but the score by chemical alone was very low. The nutritive value (ADF, NDF, digestibility, and relative feed value) of hay was also high with treatment of mechanical and chemical + mechanical, but the quality by chemical alone was similar compared with control. The quality of hay was very low when harvested at bloom stage. In conclusion, mower conditioning can enhance the field drying rate of rye, but the drying effectiveness of chemical drying agent was very low. The effect of chemical/mechanical combined treatment was very similar when compared with mechanical alone. Harvest at early heading to heading stage was recommended for high quality rye hay.
This paper investigated actual crop evapotranspiration (ETc) of tomato and paprika planted in test beds of the greenhouse. Crop water requirement (CWR) is the amount of water required to compensate ETc loss from the crop. The main objectives of the study are to assess whether the actual crop watering (ACW) was adequate CWR of tomato and paprika and which amount of ACW should be irrigated to each crop. ETc was estimated using the Penman-Monteith model (P-M) for each crop. ACW was calculated from the difference of amount of nutrient supply water and amount of nutrient drainage water. ACW and CWR of each crop were determined, compared and assessed. Results indicated CWR-tomato was around 100 to 1,200 ml/day, while CWR-paprika ranged from 100 to 500 ml/day. Comparison of ACW and CWR of each crop found that the difference of ACW and CWR are fluctuated following day of planting (DAP). However, the differences could divide into two phases, first the amount of ACWs of each crop are less than CWR in the initial phase (60 DAP) around 500 ml/day and 91 ml/day, respectively. Then, ACWs of each crop are greater than the CWR after 60 DAP until the end of cultivation approximately 400 ml/day in tomato and 178 ml/day in paprika. ETc assessment is necessary to correctly quantify crop irrigation water needs and it is an accurate short-term estimation of CWR in greenhouse for optimal irrigation scheduling. Thus, reducing ACW of tomato and paprika in the greenhouse is a recommendation. The amount of ACW of tomato should be applied from 100 to 1,200 ml/day and paprika is 100 to 500 ml/day depend on DAP.
This research aims at identifying the goshawk's possible and replaceable breeding ground by using the MaxEnt prediction model which has so far been insufficiently used in Korea, and providing evidence to expand possible protection areas for the goshawk's breeding for the future. The field research identified 10 goshawk's nests, and 23 appearance points confirmed during the 3rd round of environmental research were used for analysis. 4 geomorphic, 3 environmental, 7 distance, and 9 weather factors were used as model variables. The final environmental variables were selected through non-parametric verification between appearance and non-appearance coordinates identified by random sampling. The final predictive model (MaxEnt) was structured using 10 factors related to breeding ground and 7 factors related to appearance area selected by statistics verification. According to the results of the study, the factor that affected breeding point structure model the most was temperature seasonality, followed by distance from mixforest, density-class on the forest map and relief energy. The factor that affected appearance point structure model the most was temperature seasonality, followed by distance from rivers and ponds, distance from agricultural land and gradient. The nature of the goshawk's breeding environment and habit to breed inside forests were reflected in this modeling that targets breeding points. The northern central area which is about $189.5 km^2$(2.55 %) is expected to be suitable breeding ground. Large cities such as Cheongju and Chungju are located in the southern part of Chungcheongbuk-do whereas the northern part of Chungcheongbuk-do has evenly distributed forests and farmlands, which helps goshawks have a scope of influence and food source to breed. Appearance point modeling predicted an area of $3,071 km^2$(41.38 %) showing a wider ranging habitat than that of the breeding point modeling due to some limitations such as limited moving observation and non-consideration of seasonal changes. When targeting the breeding points, a specific predictive area can be deduced but it is difficult to check the points of nests and it is impossible to reflect the goshawk's behavioral area. On the other hand, when targeting appearance points, a wider ranging area can be covered but it is less accurate compared to predictive breeding point since simple movements and constant use status are not reflected. However, with these results, the goshawk's habitat can be predicted with reasonable accuracy. In particular, it is necessary to apply precise predictive breeding area data based on habitat modeling results when enforcing an environmental evaluation or establishing a development plan.
This study aimed to recognize characteristics of groundwater flow in fractured bedrocks based on zonal pump-ing tests, slug tests, water quality logs and borehole TV camera logs conducted on two boreholes (NJ-11 and SJ-8) in the city of Naju. Especially, the zonal pumping tests using sin91e Packer were executed to reveal groundwater flow characteristics in the fractured bedrocks with depth. On borehole NJ-11, the zonal pumping tests resulted in a flow dimension of 1.6 with a packer depth of 56.9 meters. It also resulted in lower flow dimensions as moving to shallower packer depths, reaching a flow dimension of 1 at a 24 meter packer depth. This fact indicates that uniform permissive fractures take place in deeper zones at the borehole. On borehole SJ-8, a flow dimension of 1.7 was determined at the deepest packer level (50 m). Next, a dimension of 1.8 was obtained at 32 meters of packer depth, and lastly a dimension of 1.4 at 19 meters of packer depth. The variation of flow dimension with different packer depths is interpreted by the variability of permissive fractures with depth. Zonal pumping tests led to the utilization of the Moench (1984) dual-porosity model because hydraulic characteristics in the test holes were most suitable to the fractured bedrocks. Water quality logs displayed a tendency to increase geothermal temperature, to increase pH and to decrease dissolved oxygen. In addition, there was an increasing tendency towards electrical conductance and a decreasing tendency towards dissolved oxygen at most fracture zones.
Park, Mincheol;Kwon, Oh-Kyun;Kim, Chae Min;Yun, Do Kyun;Choi, Yongkyu
Journal of the Korean Geotechnical Society
/
v.35
no.10
/
pp.47-66
/
2019
A parametric numerical analysis according to diameter, length, and N values of soil was conducted for the PHC pile socketed into weathered rock through sandy soil layers. In the numerical analysis, the Mohr-Coulomb model was applied to PHC pile and soils, and the contacted phases among the pile-soil-cement paste were modeled as interfaces with a virtual thickness. The parametric numerical analyses for 10 kinds of pile diameters were executed to obtain the load-settlement relationship and the axial load distribution according to N-values. The load-settlement curves were obtained for each load such as total load, total skin friction, skin friction of the sandy soil layer, skin friction of the weathered rock layer and end bearing resistance of the weathered rock. As a result of analysis of various load levels from the load-settlement curves, the settlements corresponding to the inflection point of each curve were appeared as about 5~7% of each pile diameter and were estimated conservatively as 5% of each pile diameter. The load at the inflection point was defined as the mobilized bearing capacity ($Q_m$) and it was used in analyses of pile bearing capacity. And SRF was appeared above average 70%, irrespective of diameter, embedment length of pile and N value of sandy soil layer. Also, skin frictional resistance of sandy soil layers was evaluated above average 80% of total skin frictional resistance. These results can be used in calculating the bearing capacity of prebored PHC pile, and also be utilized in developing the bearing capacity prediction method and chart for the prebored PHC pile socketed into weathered rock through sandy soil layers.
This study was conducted to estimate the volume growth by forest type and major species using the national forest resource inventory and to predict the final age of maturity by deriving the mean annual increment (MAI) and the current annual increment (CAI). We estimated the volume growth using the Chapman-Richards model. In the volume estimation equations by forest type, coniferous forests exhibited the highest growth. According to the estimation formula for each major species, Larix kaempferi will grow the highest among coniferous tree species and Quercus mongolica among broad-leaved tree species. And these estimation formulas showed that the fitness index was generally low, such as 0.32 for L. kaempferi and 0.21 for Quercus variabilis. In the analysis of residual amount, which indicates the applicability of the volume estimation formula, the estimates of the estimation formula tended to be underestimated in about 30 years or more, but most of the residuals were evenly distributed around zero. Therefore, these estimation formulas have no difficulty estimating the volume of actual forest species in Korea. The maximum age attained by calculating MAI was 34 years for P. densiflora, 35 years for L. kaempferi, and 31 years for P. rigida among coniferous tree species. In broad-leaved tree species, we discovered that the maximum age was 32 years for Q. variabilis, 30 years for Q. acutissima, and 29 years for Q. mongolica. We calculated MAI and CAI to detect the point at which these two curves intersected. This point was defined by the maximum volume harvesting age. These results revealed no significant difference between the current standard cutting age in public and private forests recommended by the Korea Forest Service, supporting the reliability of forestry policy data.
Ha, Gwangsu;Shin, Su-Jin;Jeong, Seong-Yeop;Yang, HoYeon;Im, Sua;Heo, JuHee;Yang, Hee-Jong;Jeong, Do-Youn
Journal of Life Science
/
v.29
no.8
/
pp.861-870
/
2019
This study was undertaken to establish optimum medium compositions for cost-effective mannitol production by Leuconostoc mesenteroides SRCM201425 isolated from kimchi. L. mesenteroides SRCM21425 from kimchi was selected for efficient mannitol production based on fructose analysis and identified by its 16S rRNA gene sequence, as well as by carbohydrate fermentation pattern analysis. To enhance mannitol production by L. mesenteroides SRCM201425, the effects of carbon, nitrogen, and mineral sources on mannitol production were first determined using Plackett-Burman design (PBD). The effects of 11 variables on mannitol production were investigated of which three variables, fructose, sucrose, and peptone, were selected. In the second step, each concentration of fructose, sucrose, and peptone was optimized using a central composite design (CCD) and response surface analysis. The predicted concentrations of fructose, sucrose, and peptone were 38.68 g/l, 30 g/l, and 39.67 g/l, respectively. The mathematical response model was reliable, with a coefficient of determination of $R^2=0.9185$. Mannitol production increased 20-fold as compared with the MRS medium, corresponding to a mannitol yield 97.46% when compared to MRS supplemented with 100 g/l of fructose in flask system. Furthermore, the production in the optimized medium was cost-effective. The findings of this study can be expected to be useful in biological production for catalytic hydrogenation causing byproduct and additional production costs.
The purpose of this study is to analyze the cause of high PM2.5 mass concentrations in Cheongju for the period of non-Asian dust days using the weather chart, the stream lines at 850 hPa, the backward trajectory, and the weather and air quality model. As a result of analyzing the time series of PM2.5 concentrations and weather charts for the episodic days in Cheongju, the weather patterns were shown in related to long-range transport of PM2.5 from China or surrounding areas. In fact, in the PM2.5 time series, 60-80 ㎍ m-3, which is more than 2-3 times higher than the concentration attributed to Cheongju activities, was observed as a background concentration related to long-range transport. The distribution of high PM2.5 concentration was typically dependent on the locations of the high and low pressures above the ground while the upper jet stream passed through the Korean Peninsula. Consequently, the high PM2.5 concentration in Cheongju is due to massive air pollutants in the form of smog originated from industrial, household and energy combustion sources of Beijing and other nearby regions of China. These air pollutants move along a fast zonal wind caused by the atmospheric pressure arrangement. high concentration of PM2.5 in Cheongju City is because the mass of air pollutants in the form of smog generated from industrial, household and energy combustion origins in Beijing or other nearby regions of China move along a fast wind speed zone according to the atmospheric pressure arrangement of long-distance transportation. Air pollutants including PM2.5 show an M-shaped pattern that passes through the topography of the Cheongju basin from north to south as a belt or band-shaped pollutant. The ground high pressure according to the above-ground high pressure expansion area and cut-off low or low pressure arrangement, or the bands in the form of river stems appear in a gradual incremental pattern that changes into a U-shape under the influence of the wind.
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