• Microbiology and Biotechnology Letters
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• v.18 no.1
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• pp.103-108
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• 1990

The enzymatic studies on the methylglyoxal metabolism in yeast and bacterial cells indicated that organisms are equipped with the common and manifold systems for the detoxification of methylglyoxal. Among these systems, the glyoxalase I is the most important route for methylglyoxal detoxification. The molecular structure of glyoxalase I is apparently distinct from the enzyme sources, and zinc ion is an essential cofactor in enzyme activity. The gene for Pseudomonas putida glyoxalase I functioned as a scavenger of methylglyoxal and regulated the cell size of the bacterium. Comparison of the nucleotide sequence of the P. putida glyoxalase I gene with the N-terminal amino acid sequence of the purified enzyme revealed that the N-terminal methionine residue was removed after translation. Possible physiological role of glyoxalase I was also discussed.

• Korean journal of applied entomology
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• v.30 no.2
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• pp.106-110
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• 1991

The mode of inheritance of resistance to fenvalerate in the diamondback moth (Plutella xylostella L.) was studied using insecticide susceptibility and mixed function oxidase tests. Tehre were no differences in the concentration-mortality relationships between $F_1$ progenies $(S_{female}\timesR_{male},\;R_{female}\timesS_{male})$ obtained from reciprocal crosses with the susceptible and fenvalerate-selected strains(R), indicating the absence of sex-linked inheritance. Degree of dominance of the $F_1$ progenies $(S_{female}\timesR_{male},\;R_{female}\timesS_{male})$ were -0.50 and -0.46, respectively, in the insecticide susceptibility test and -0.85 and -0.81, respectively, in the mixed function oxidase test. These results suggest that inheritance of fenvalerate resistance is controlled by and incompletely recessive autosomal gene.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.196-206
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• 2018

Dramatic increase in global population accompanied by rapid industrialization in developing countries has led to serious environmental, food, energy, and health problems. The Food and Agriculture Organization of the United Nations has estimated world population will increase to 9.7 billion by 2050 and require approximately 1.7 times more food, and more than 3.5 times energy than that of today. Particularly, sweetpotato is easy to cultivate in unfavorable conditions such as heat, drought, high salt, and marginal lands. In this respect, sweetpotato is an industrially valuable starch crop. To replace crops associated with these food and energy problems, it is necessary to develop new crops with improved nutrients and productivity, that can be grown on marginal lands, including desertification areas using plant biotechnology. For this purpose, exploring useful genes and developing genetically modified crops are essential strategies. Currently, sweetpotato [Ipomoea batatas (L.) Lam.] have been re-evaluated as the best health food and industrial crop that produces starch and low molecular weight antioxidants, such as vitamin A, vitamin E, anthocyanins and carotenoids. This review will focus on the current status of research on sweetpotato biotechnology on omics including genome sequencing, transcriptome, proteomics and molecular breeding. In addition, prospects on molecular breeding of sweetpotato on marginal lands for sustainable development were described.

• Environmental Analysis Health and Toxicology
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• v.23 no.3
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• pp.195-200
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• 2008

Hydroxylated polychlorinated biphenyl (OH-PCBs)는 PCB의 CYP-dependent oxidation의 대사물로서 잠재적 독성이 강하고 지질친화성을 가지며 생물군에 지속적인 축적성을 나타낸다. OH-PCBs의 해독화 효능을 조사하기 위하여 channel catfish 간에서 glucuronidation을 통한 해독작용 가능성을 평가하고 biphenyl 구조에 다양한 염소치환의 구조적 차이점에 따른 영향을 비교 분석하여 보았다. Kinetic parameters에서 $K_m$과 $V_{max}$는 $192{\sim}871{\mu}M$, $869{\sim}1774$ pmo1/min/mg으로써 4'-OH-PCB35와 4'-OH-PCB69이 가장 높은 속도의 glucuronidation을 나타냈으며, 구조적 차이점에서 phenolic group에 한 개의 염소치환이 존재할 경우보다 두 개의 염소치환이 존재할 경우에 OH-PCBs(p<0.001)의 glucuronidation에 대한 $V_{max}$를 현저하게 낮추는 결과를 보였다.

• The Korean Journal of Pharmacology
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• v.26 no.2
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• pp.185-192
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• 1990

The present work was undertaken to investigate the protective effect of diallyl disulfide on the bromobenzene toxicity in mice. It was observed that the aniline hydroxylase and epoxide hydrolase activities were not changed by the treatment of diallyl disulfide for 5 days. But glutathione S-transferase activity was significantly increased. A striking enhancement of serum alanine aminotransferase activity and hepatic lipid peroxide content after bromobenzene administration was markedly decreased by diallyl disulfide pretreatment. These results indicate that the inducing effects of diallyl disulfide on the bromobenzene intermediate detoxifying enzyme such as glutathione S-transferase are believed to be a possible protective mechanism for the bromobenzene toxicity in mice.

• The Korean Journal of Mycology
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• v.23 no.1 s.72
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• pp.71-79
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• 1995

The mechanism of cadmium adaptation and detoxification in Rhizopus oryzae was investigated. The lag phase was lengthened as the concentration of cadmium increased. Detoxication of cadmium were postulated to be primarily operated by the induction of two cadmium binding proteins and increment of inorganic polyphosphate pools in adaptation phase. After adaptation, inorganic polyphosphate system has been involved in turnover and compartmentalization. The secondary system for cadmium adaptation and detoxification might be derepression of ACPase activity and the synthesis of phosphatidyl serine. It has been considered that the overall changes for cadmium adaptation and detoxfication eventually influence on the morphology, resulting in the dispersed filamentous type which may be the most advantageous form.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2001.06a
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• pp.171-174
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• 2001

식물의 대사과정을 통하여 흡수된 대기오염물질은 많은 단계의 해독과정 거치게 된다. SO$_2$ 의 경우는 세포질에서 그 대부분이 SO$_{3}$$^{2-}$ 로 용해되고 일부는 HSO$_{3}$ $^{-}$의 형태로 용해된다. 용해된 SO$_{3}$$^{2-}$ 는 아황산산화효소에 의해서 엽록체에서 생성된 $O_{2}$$^{-}$와 산화 반응하여 비교적 독성이 낮은 SO$_{4}$$^{2-}$ 로 변화한다.(중략)

• Proceedings of the Korean Information Science Society Conference
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• 2003.10b
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• pp.802-804
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• 2003

2000년대 초 인간 지놈 프로젝트의 완성으로 새로운 포스트-지놈 시대를 맞이하여, 유전자에 대한 해독보다는 인간의 모든 대사와 질병에 직접관여 하고 있는 단백질의 구조와 기능에 대해 많은 관심과 연구가 이루어지고 있다. 특히, 특정 단백질들은 암과 같은 불치병에 직접관여 하고 있으므로 이러한 단백질들의 기능과 구조에 대한 예측 성능의 향상은 새로운 신약 개발에 큰 도움이 될 것이다. 본 논문은 기계학습(Machine Learning)의 한 분야인 HMM(Hidden Markov Model)을 이용하여 $\beta$-barrel 형태로 막횡단하는 단백질의 특성과 기능으로부터 막횡단하는 부위가 존재하는지 여부를 예측하는 프로그램을 구현했다.

• Food Science of Animal Resources
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• v.25 no.4
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• pp.373-382
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• 2005

This study was performed to investigate the effect of pork on the cadmium detoxification in rats. Ninety male Sprague-Dawley strains were divided into five stoups based on cadmium treatment(0, 25, 50, 100, 250 ppm) and cadmium intoxication was observed for 8 weeks. During following 8 weeks, casein was replaced by Pork and the effect of pork was compared with casein. During intoxicating, the growth mte was lessened. Feed efficiency ratio was significantly low, as cadmium intake was high Discontinuing cadmium feeding, the body weights were relieve4 Pork-fed groups seemed to have higher body weight than casein-fed groups. During intoxication, hemoglobin and hematocrit were dependent upon cadmium intake. However, they became normal at detocification stage. Cadmium accumulation of the pour-fed group was lower than that of casein. Especially, the factors which affected the cadmium content in kidney were Cd(p<0.001) and $Cd^*pork(p<0.001)$. Metallothionein(MT) was increased with cadmium, and MT was not likely to be affected by pork. This study may provide scientific background of pork for detoxification from heavy metals such as cadmium.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.702-708
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• 1993

The study was attempted to elucidate the mechanism of GE-132(100mg/kg, p.o. for 6 weeks) on the metabolism of bromobenzene (460mg/kg, i.p. bid, for 2 days), which has potent carcinogenicity, mutagenicity and hepatotoxicity. It showed that activities of cytochrome p-450, aminopyrine demethylase and aniline hydroxylase, which have epoxide generating property, were not changed by GE-132 treatment. On the other hand, epoxide hydrolase was not changed but that glutathione S-transferase was significantly increased by GE-132 treatment. And also ${\gamma}-glutamylcysteine$ synthetase was not changed following the GE-132 treatment, but the activity of glutathione reductase was significantly increased. The level of hepatic glutathione which was decreased by bromobenzene recovered markedly by GE-132 pretreatment. It is concluded that the mechanism for the observed effect of GE-132 on bromobenzene metabolism is due to the induction of glutathione S-transferase.